LOCUS       V008482                 7695 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008482
VERSION     V008482
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7695)
  AUTHORS   Kraft C.
  TITLE     A toolbox for the analysis of weak and low abundant molecule
            interactions in vivo by M-Track in budding yeast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7695)
  AUTHORS   Brezovich A, Schuschnig M, Ammerer G, Kraft C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2014) Department of Biochemistry and Cell Biology,
            MFPL, University of Vienna, Dr. Bohr Gasse 9, Vienna 1030, Austria
REFERENCE   3  (bases 1 to 7695)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7695)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-AUG-2014) Department of Biochemistry and Cell Biology, MFPL,
            University of Vienna, Dr. Bohr Gasse 9, Vienna 1030, Austria"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7695
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        224..328
                     /label="AmpR promoter"
     CDS             329..1186
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1360..1948
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2236..2257
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2272..2302
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2310..2326
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2334..2350
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2371..2389
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             3076..4575
                     /gene="ATG18"
                     /label="Autophagy-related protein 18"
                     /note="Autophagy-related protein 18 from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P43601"
     CDS             4942..5031
                     /label="3xHA"
                     /note="three tandem HA epitope tags"
     terminator      5053..5300
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     promoter        complement(5319..5337)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5347..5363)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      5504..5959
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(6093..6893)
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(6894..7109)
                     /label="URA3 promoter"
     misc_feature    complement(join(7379..7695,1..187))
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
ORIGIN
        1 ggcattaata cctgagagca ggaagagcaa gataaaaggt agtatttgtt ggcgatcccc
       61 ctagagtctt ttacatcttc ggaaaacaaa aactattttt tctttaattt ctttttttac
      121 tttctatttt taatttatat atttatatta aaaaatttaa attataatta tttttatagc
      181 acgtgatgaa aaggacccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      241 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      301 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      361 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      421 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      481 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      541 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      601 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      661 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      721 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      781 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      841 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
      901 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      961 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1021 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1081 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1141 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1201 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1261 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1321 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1381 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1441 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1501 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1561 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1621 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1681 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1741 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1801 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1861 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1921 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1981 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2041 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2101 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     2161 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     2221 tgagcgcaac gcaattaatg tgagttacct cactcattag gcaccccagg ctttacactt
     2281 tatgcttccg gctcctatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     2341 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     2401 ggagctccac cgcggtggcg gccgctaacg tagttatccg aatgcataaa ttagactagt
     2461 atgtgacaac gctaaacatg cgttccccag aaccacctgc ggttgttgag aggaataggt
     2521 tttgcgcttg tctatggaca ctttcttgaa ctcaatccaa ctgagcaata cttaacaata
     2581 ttgcgattac ttgttcttac ataaagagta agaaatactt gcgaaataaa cggtaatcaa
     2641 tcaaatttag ggtcataagt tgtattcgca caatatgcat aagcactgac tcaagaaaga
     2701 agatggtttt cgaatgggga gctagcaaaa agagtcaccc ctaagtataa aaactgaatt
     2761 acaaagctct tcgactactt tctgcaagta aatcattcgg aagtgcgaca atataatgac
     2821 aaaagacgat aagttacttc tattaattga actttaagta tttggctttt attttttttt
     2881 tttcattttc ttactcgcgc ttttctttat taagggcttt ctcaattaga tgttaataat
     2941 gtcataaggc atcgttattc cgctttatat tggtaactca gcagtggtca ttgccttcgt
     3001 tcacgaccct cccttattaa tcagttagta atagtgttcc agttaactct gtatcctttt
     3061 ctcttcggcc tgacaatgtc tgattcatca cctactatca actttattaa tttcaatcaa
     3121 accggaacgt gtatttccct tggaacgtcg aaaggtttca aaatattcaa ttgtgagccc
     3181 ttcggaaaat tttattcaga ggacagtggg ggctatgcta tcgtcgagat gttgttctcc
     3241 acctcgttac tagccctcgt tgggataggc gatcaacctg cgctttcacc aaggagattg
     3301 cgtataatca acacaaaaaa acattctatt atctgtgagg tgactttccc tacttctatt
     3361 ctgagtgtga aaatgaataa gtctcgattg gtggtacttt tacaagagca gatttatatt
     3421 tatgatatca acaccatgag actattgcat actatagaaa caaaccctaa cccacgtggc
     3481 cttatggcta tgtctccttc ggtagccaac agctatttag tgtatccatc accaccaaaa
     3541 gttattaact ccgaaattaa agctcatgcc accacaaaca atatcacatt gtcagttggt
     3601 ggcaacacag agaccagttt caagagagat cagcaagatg ctggccatag tgacattagc
     3661 gacttggatc agtattcgag ctttactaag agggatgatg cggatccaac aagcagtaac
     3721 ggcggtaaca gcagtataat aaagaatggt gatgtgatcg tattcaactt ggaaacatta
     3781 cagccaacca tggtcatcga agctcataag ggcgagattg ctgcaatggc aattagtttt
     3841 gatgggacac taatggctac cgcctctgat aaaggtacta tcatcagggt ctttgacatt
     3901 gaaacgggtg ataagatcta ccaattcagg agagggacgt acgcgacaag aatttactcc
     3961 atatcattca gtgaagatag ccagtacttg gcggttaccg gctcttccaa aaccgtgcat
     4021 atcttcaaat tggggcattc aatgagcaac aataaactag acagcgatga tagcaacatg
     4081 gaagaagctg cagccgatga ttcatcgctc gataccacca gtatcgatgc gctgagtgac
     4141 gaggaaaacc cgacaagact cgcaagagaa ccatatgtgg atgcatcaag aaagacaatg
     4201 ggtaggatga tacgttactc ttctcaaaag ctatcccgaa gagctgccag aacattgggt
     4261 cagattttcc ccatcaaagt tacatcgttg ttggaatcct cgcgccattt tgcgtctttg
     4321 aaacttcccg ttgaaaccaa ttcccatgta atgaccatat caagtatagg ctctccaata
     4381 gatatagaca catccgagta tccggaactc ttcgaaactg gcaattccgc aagtacagag
     4441 tcctaccatg agcctgttat gaagatggtc cccatcaggg tcgtttcctc ggatggatac
     4501 ctatacaact ttgttatgga cccggagaga ggcggcgatt gcttaatatt gtcacagtat
     4561 tccatcttga tggacctgca ggacgacctc gagtctaggg gatccccggg gatcccgaat
     4621 tcggctcgta ctaagcagac cgctcgcaag tccaccggcg gcaaggcccc gcgcaagcag
     4681 ctggcccaga tcccgaattc ggctcgtact aagcagaccg ctcgcaagtc caccggcggc
     4741 aaggccccgc gcaagcagct ggcccagatc ccgaattcgg ctcgtactaa gcagaccgct
     4801 cgcaagtcca ccggcggcaa ggccccgcgc aagcagctgg cccagatccc gaattcggct
     4861 cgtactaagc agaccgctcg caagtccacc ggcggcaagg ccccgcgcaa gcagctggcc
     4921 cagatctgcg gacgcatctt ttacccatac gatgttcctg actatgcggg ctatccctat
     4981 gacgtcccgg actatgcagg atcctatcca tatgacgttc cagattacgc tgctcagtgc
     5041 gtcgacctcg agtcatgtaa ttagttatgt cacgcttaca ttcacgccct ccccccacat
     5101 ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt
     5161 ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt
     5221 acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag gttttgggac
     5281 gctcgaaggc tttaatttgc ggccggtacc caattcgccc tatagtgagt cgtattacgc
     5341 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     5401 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     5461 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc
     5521 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5581 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5641 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5701 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5761 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5821 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     5881 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     5941 atattaacgt ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     6001 cacaccgcat agggtaataa ctgatataat taaattgaag ctctaatttg tgagtttagt
     6061 atacatgcat ttacttataa tacagttttt tagttttgct ggccgcatct tctcaaatat
     6121 gcttcccagc ctgcttttct gtaacgttca ccctctacct tagcatccct tccctttgca
     6181 aatagtcctc ttccaacaat aataatgtca gatcctgtag agaccacatc atccacggtt
     6241 ctatactgtt gacccaatgc gtctcccttg tcatctaaac ccacaccggg tgtcataatc
     6301 aaccaatcgt aaccttcatc tcttccaccc atgtctcttt gagcaataaa gccgataaca
     6361 aaatctttgt cgctcttcgc aatgtcaaca gtacccttag tatattctcc agtagatagg
     6421 gagcccttgc atgacaattc tgctaacatc aaaaggcctc taggttcctt tgttacttct
     6481 tctgccgcct gcttcaaacc gctaacaata cctgggccca ccacaccgtg tgcattcgta
     6541 atgtctgccc attctgctat tctgtataca cccgcagagt actgcaattt gactgtatta
     6601 ccaatgtcag caaattttct gtcttcgaag agtaaaaaat tgtacttggc ggataatgcc
     6661 tttagcggct taactgtgcc ctccatggaa aaatcagtca agatatccac atgtgttttt
     6721 agtaaacaaa ttttgggacc taatgcttca actaactcca gtaattcctt ggtggtacga
     6781 acatccaatg aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa tagcttggca
     6841 gcaacaggac taggatgagt agcagcacgt tccttatatg tagctttcga catgatttat
     6901 cttcgtttcc tgcatgtttt tgttctgtgc agttgggtta agaatactgg gcaatttcat
     6961 gtttcttcaa cactacatat gcgtatatat accaatctaa gtctgtgctc cttccttcgt
     7021 tcttccttct gttcggagat taccgaatca aaaaaatttc aaagaaaccg aaatcaaaaa
     7081 aaagaataaa aaaaaaatga tgaattgaat tgaaaagctg tggtatggtg cactctcagt
     7141 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     7201 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     7261 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     7321 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttaggacgga
     7381 tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat gatggaataa tttgggaatt
     7441 tactctgtgt ttatttattt ttatgttttg tatttggatt ttagaaagta aataaagaag
     7501 gtagaagagt tacggaatga agaaaaaaaa ataaacaaag gtttaaaaaa tttcaacaaa
     7561 aagcgtactt tacatatata tttattagac aagaaaagca gattaaatag atatacattc
     7621 gattaacgat aagtaaaatg taaaatcaca ggattttcgt gtgtggtctt ctacacagac
     7681 aagatgaaac aattc
//
