LOCUS       V008483                 8907 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008483
VERSION     V008483
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8907)
  AUTHORS   Kraft C.
  TITLE     A toolbox for the analysis of weak and low abundant molecule
            interactions in vivo by M-Track in budding yeast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8907)
  AUTHORS   Brezovich A, Schuschnig M, Ammerer G, Kraft C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2014) Department of Biochemistry and Cell Biology,
            MFPL, University of Vienna, Dr. Bohr Gasse 9, Vienna 1030, Austria
REFERENCE   3  (bases 1 to 8907)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8907)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-AUG-2014) Department of Biochemistry and Cell Biology, MFPL,
            University of Vienna, Dr. Bohr Gasse 9, Vienna 1030, Austria"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    821..842
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        857..887
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    895..911
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     919..935
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        956..974
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             1647..2315
                     /gene="FSH2"
                     /label="Family of serine hydrolases 2"
                     /note="Family of serine hydrolases 2 from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     Q05015"
     CDS             2340..2369
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             2376..2405
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     CDS             2412..2441
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     CDS             2460..2489
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     CDS             2496..2525
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     CDS             2532..2561
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     CDS             2580..2609
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     CDS             2616..2645
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     CDS             2652..2681
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     misc_feature    2715..3707
                     /label="HKMT"
                     /note="HKMT"
     terminator      3723..3970
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     promoter        complement(3989..4007)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4017..4033)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      4178..4633
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4933..5337
                     /label="LEU2 promoter"
     CDS             5350..6441
                     /label="LEU2"
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     misc_feature    complement(7176..7679)
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        7716..7820
                     /label="AmpR promoter"
     CDS             7821..8678
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8852..8907
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
       61 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
      121 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
      181 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
      241 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      301 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      361 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      421 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      481 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      541 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      601 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      661 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
      721 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
      781 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tacctcactc
      841 attaggcacc ccaggcttta cactttatgc ttccggctcc tatgttgtgt ggaattgtga
      901 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
      961 accctcacta aagggaacaa aagctggagc tccaccgcgg tggcggccgc gtgagagaga
     1021 aaatgcgaat ggttccaaca tccacaaaat ccacattgta gacacatgaa agttgcacca
     1081 gagtttattt cgtggcatgt accacatttc atggtgttta agaatttttc cttgggtacg
     1141 gaatgattta atatatacct ggcagcattg cacgttttta gaaccccatc tttagacttt
     1201 tcattctgaa atacttgctg tatatgtgga caaatgctca taattattaa taacaaataa
     1261 aaaaagcagg gtttcaagta ggacgacgag gaccgaagct gtagttttcc cttttgatcc
     1321 gatcaataaa tagtattcaa aattatgaca gccagttgaa agaaatggac taactggtct
     1381 caaaagaaaa cctttattat tatccgtaaa atatcttttt agaattaatc aggtccacta
     1441 atataatccc tttgctatta cgacagcttt ctagcccata tacaatataa attattgatg
     1501 ctttttacac actataatcg aggatttttt ttcacttgaa aaattcaaag aaaagcaaaa
     1561 aaaggaagaa tagagaagaa aaaaaaggta taagtgaaga aagaatgtgc aatactacaa
     1621 gactataaga tttaatagct gtaaccatga cgaagaatgt attgatgtta catgggcttg
     1681 cgcagtcagg tgactacttt gcctctaaga caaaggggtt tcgcgccgaa atggaaaagt
     1741 taggatataa actctactat ccaacagcac ccaatgaatt tcccccagct gatgttcctg
     1801 atttcttggg tgaggtaatt gctgatgcac ctggggatgg tgagaatacc ggtgtgcttg
     1861 cgtggctaga gaatgaccct tccaccggtg gctatttcat accgcagacg acaatcgact
     1921 atttacacaa ttatgtcctt gaaaatggtc catttgcagg tattgtagga tttagtcaag
     1981 gagccggtgt agctggatac ctagcaacgg atttcaatgg tttgttgggc cttacaacag
     2041 aagaacagcc gccgctagaa tttttcatgg cggtcagtgg tttcaggttc caaccacaac
     2101 agtatcaaga acagtacgac ctgcatccaa tatcggtccc atctctacat gttcaaggag
     2161 aacttgacac tatcacagag ccggccaaag tgcaaggcct atataactct tgtacggaag
     2221 actctcgcac tttactaatg cactctgggg gccactttgt gccgaactca agagggtttg
     2281 tgagaaaagt ggctcaatgg ctccagcagt tgaccctgca gggttctgct gctagtggtg
     2341 aacaaaagtt gatttctgaa gaagatttga acggtgaaca aaagctaatc tccgaggaag
     2401 acttgaacgg tgaacaaaaa ttaatctcag aagaagactt gaacggatcc tctagaggtg
     2461 aacaaaagtt gatttctgaa gaagatttga acggtgaaca aaagctaatc tccgaggaag
     2521 acttgaacgg tgaacaaaaa ttaatctcag aagaagactt gaacggatcc tctagaggtg
     2581 aacaaaagtt gatttctgaa gaagatttga acggtgaaca aaagctaatc tccgaggaag
     2641 acttgaacgg tgaacaaaaa ttaatctcag aagaagactt gaacggatcc actctgcagg
     2701 agtctagggg atcctgtgtg cgtatcctca agcagttcca caaggactta gaaagggagc
     2761 tgctccggcg gcaccaccgg tcaaagaccc cccggcacct ggacccaagc ttggccaact
     2821 acctggtgca gaaggccaag cagaggcggg cgctccgtcg ctgggagcag gagctcaatg
     2881 ccaagcgcag ccatctggga cgcatcactg tagagaatga ggtggacctg gacggccctc
     2941 ctcgggcctt cgtgtacatc aatgagtacc gtgttggtga gggcatcacc ctcaaccagg
     3001 tggctgtggg ctgcgagtgc caggactgtc tgtgggcacc cactggaggc tgctgcccgg
     3061 gggcgtcact gcacaagttt gcctacaatg accagggcca ggtgcggctt cgagccgggc
     3121 tgcccatcta cgagtgcaac tcccgctgcc gctgcggcta tgactgccca aatcgtgtgg
     3181 tacagaaggg tatccgatat gacctctgca tcttccgcac ggatgatggg cgtggctggg
     3241 gcgtccgcac cctggagaag attcgcaaga acagcttcgt catggagtac gtgggagaga
     3301 tcattacctc agaggaggca gagcggcggg gccagatcta cgaccgtcag ggcgccacct
     3361 acctctttga cctggactac gtggaggacg tgtacaccgt ggatgccgcc tactatggca
     3421 acatctcccg ctttgtcaac cacagttgtg accccaacct acaggtgtac aacgtcttca
     3481 tagacaacct tgacgagcgg ctgccccgca tcgctttctt tgccacaaga accatccggg
     3541 caggcgagga gctcaccttt gattacaaca tgcaagtgga ccccgtggac atggagagca
     3601 cccgcatgga ctccaacttt ggcctggctg ggctccctgg ctcccctaag aagcgggtcc
     3661 gtattgaatg caagtgtggg actgagtcct gccgcaaata cctcttctag gtcgacctcg
     3721 agtcatgtaa ttagttatgt cacgcttaca ttcacgccct ccccccacat ccgctctaac
     3781 cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt ttatagttat
     3841 gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt acagacgcgt
     3901 gtacgcatgt aacattatac tgaaaacctt gcttgagaag gttttgggac gctcgaaggc
     3961 tttaatttgc ggccggtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     4021 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     4081 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     4141 tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag cggcgcatta
     4201 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4261 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4321 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4381 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4441 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4501 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     4561 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     4621 acgtttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     4681 gcatatcgac ggtcgaggag aacttctagt atatccacat acctaatatt attgccttat
     4741 taaaaatgga atcccaacaa ttacatcaaa atccacattc tcttcaaaat caattgtcct
     4801 gtacttcctt gttcatgtgt gttcaaaaac gttatattta taggataatt atactctatt
     4861 tctcaacaag taattggttg tttggccgag cggtctaagg cgcctgattc aagaaatatc
     4921 ttgaccgcag ttaactgtgg gaatactcag gtatcgtaag atgcaagagt tcgaatctct
     4981 tagcaaccat tatttttttc ctcaacataa cgagaacaca caggggcgct atcgcacaga
     5041 atcaaattcg atgactggaa attttttgtt aatttcagag gtcgcctgac gcatatacct
     5101 ttttcaactg aaaaattggg agaaaaagga aaggtgagag gccggaaccg gcttttcata
     5161 tagaatagag aagcgttcat gactaaatgc ttgcatcaca atacttgaag ttgacaatat
     5221 tatttaagga cctattgttt tttccaatag gtggttagca atcgtcttac tttctaactt
     5281 ttcttacctt ttacatttca gcaatatata tatatatttc aaggatatac cattctaatg
     5341 tctgccccta tgtctgcccc taagaagatc gtcgttttgc caggtgacca cgttggtcaa
     5401 gaaatcacag ccgaagccat taaggttctt aaagctattt ctgatgttcg ttccaatgtc
     5461 aagttcgatt tcgaaaatca tttaattggt ggtgctgcta tcgatgctac aggtgtccca
     5521 cttccagatg aggcgctgga agcctccaag aaggttgatg ccgttttgtt aggtgctgtg
     5581 ggtggtccta aatggggtac cggtagtgtt agacctgaac aaggtttact aaaaatccgt
     5641 aaagaacttc aattgtacgc caacttaaga ccatgtaact ttgcatccga ctctctttta
     5701 gacttatctc caatcaagcc acaatttgct aaaggtactg acttcgttgt tgtcagagaa
     5761 ttagtgggag gtatttactt tggtaagaga aaggaagacg atggtgatgg tgtcgcttgg
     5821 gatagtgaac aatacaccgt tccagaagtg caaagaatca caagaatggc cgctttcatg
     5881 gccctacaac atgagccacc attgcctatt tggtccttgg ataaagctaa tgttttggcc
     5941 tcttcaagat tatggagaaa aactgtggag gaaaccatca agaacgaatt ccctacattg
     6001 aaggttcaac atcaattgat tgattctgcc gccatgatcc tagttaagaa cccaacccac
     6061 ctaaatggta ttataatcac cagcaacatg tttggtgata tcatctccga tgaagcctcc
     6121 gttatcccag gttccttggg tttgttgcca tctgcgtcct tggcctcttt gccagacaag
     6181 aacaccgcat ttggtttgta cgaaccatgc cacggttctg ctccagattt gccaaagaat
     6241 aaggttgacc ctatcgccac tatcttgtct gctgcaatga tgttgaaatt gtcattgaac
     6301 ttgcctgaag aaggtaaggc cattgaagat gcagttaaaa aggttttgga tgcaggtatc
     6361 agaactggtg atttaggtgg ttccaacagt accaccgaag tcggtgatgc tgtcgccgaa
     6421 gaagttaaga aaatccttgc ttaaaaagat tctctttttt tatgatattt gtacataaac
     6481 tttataaatg aaattcataa tagaaacgac acgaaattac aaaatggaat atgttcatag
     6541 ggtagacgaa actatatacg caatctacat acatttatca agaaggagaa aaaggaggat
     6601 agtaaaggaa tacaggtaag caaattgata ctaatggctc aacgtgataa ggaaaaagaa
     6661 ttgcacttta acattaatat tgacaaggag gagggcacca cacaaaaagt taggtgtaac
     6721 agaaaatcat gaaactacga ttcctaattt gatattggag gattttctct aaaaaaaaaa
     6781 aaatacaaca aataaaaaac actcaatgac ctgaccattt gatggagttt aagtcaatac
     6841 cttcttgaac catttcccat aatggtgaaa gttccctcaa gaattttact ctgtcagaaa
     6901 cggccttacg acgtagtcga tatggtgcac tctcagtaca atctgctctg atgccgcata
     6961 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     7021 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     7081 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     7141 ggttaatgtc atgataataa tggtttctta ggacggatcg cttgcctgta acttacacgc
     7201 gcctcgtatc ttttaatgat ggaataattt gggaatttac tctgtgttta tttattttta
     7261 tgttttgtat ttggatttta gaaagtaaat aaagaaggta gaagagttac ggaatgaaga
     7321 aaaaaaaata aacaaaggtt taaaaaattt caacaaaaag cgtactttac atatatattt
     7381 attagacaag aaaagcagat taaatagata tacattcgat taacgataag taaaatgtaa
     7441 aatcacagga ttttcgtgtg tggtcttcta cacagacaag atgaaacaat tcggcattaa
     7501 tacctgagag caggaagagc aagataaaag gtagtatttg ttggcgatcc ccctagagtc
     7561 ttttacatct tcggaaaaca aaaactattt tttctttaat ttcttttttt actttctatt
     7621 tttaatttat atatttatat taaaaaattt aaattataat tatttttata gcacgtgatg
     7681 aaaaggaccc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     7861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     7921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     7981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     8041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     8101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     8161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     8221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     8281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     8341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     8401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     8461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     8521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     8581 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     8641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     8701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     8761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     8881 gctgcttgca aacaaaaaaa ccaccgc
//
