LOCUS       40924_10876        5710 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCK902, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Kraft C.
  TITLE     A toolbox for the analysis of weak and low abundant molecule 
            interactions in vivo by M-Track in budding yeast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5710)
  AUTHORS   Brezovich A, Schuschnig M, Ammerer G, Kraft C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2014) Department of Biochemistry and Cell Biology,
            MFPL, University of Vienna, Dr. Bohr Gasse 9, Vienna 1030, Austria
REFERENCE   3  (bases 1 to 5710)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5710)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2014) Department of Biochemistry and Cell Biology, MFPL, 
            University of Vienna, Dr. Bohr Gasse 9, Vienna 1030, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    74..95
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        110..140
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    148..164
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     172..188
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        209..227
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    261..307
                     /label=GFP region
                     /note="GFP region"
     CDS             314..340
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     CDS             347..373
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     CDS             467..487
                     /codon_start=1
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
                     /translation="ENLYFQG"
     CDS             518..691
                     /codon_start=1
                     /label=ProtA
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             695..865
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="DNKFNKEQQNAFYEILHLPNLNEEQRNALIQSLKDDPSQSANLLA
                     EAKKLNDAQAPK"
     misc_feature    932..1225
                     /label=4x Histone3
                     /note="4x Histone3"
     CDS             1250..1339
                     /codon_start=1
                     /label=3xHA
                     /note="three tandem HA epitope tags"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      1392..1639
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        1706..1921
                     /label=URA3 promoter
     CDS             1922..2722
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     terminator      2855..3052
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(3105..3123)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3133..3149)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            3221..3289
                     /gene="LacZ alpha"
                     /label=LacZ alpha
     rep_origin      complement(3290..3745)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3772..3876
                     /label=AmpR promoter
     CDS             3877..4734
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4908..5496
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
       61 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
      121 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
      181 gctatgacca tgattacgcc aagctcgaaa ttaaccctca ctaaagggaa caaaagctga
      241 tcgaattcct gcagcccggg ggatccccgg gttaattaac agtaaaggag aagaactttt
      301 cactggactc gagtatccgt atgacgtccc ggactatgca ggatcctatc catatgacgt
      361 tccagattac gctctgcagg aattcgatat caagcttgcg gccgccctgc aggagaaaat
      421 ctcatcctcc ggggcacttg attatgatat tccaactact gctagcgaga atttgtattt
      481 tcagggagaa ttcggccttg cgcaacacga tgaagccgtg gacaacaaat tcaacaaaga
      541 acaacaaaac gcgttctatg agatcttaca tttacctaac ttaaacgaag aacaacgaaa
      601 cgccttcatc caaagtttaa aagatgaccc aagccaaagc gctaaccttt tagcagaagc
      661 taaaaagcta aatgatgctc aggcgccgaa agtagacaac aaattcaaca aagaacaaca
      721 aaacgcgttc tatgagatct tacatttacc taacttaaac gaagaacaac gaaacgccct
      781 catccaaagt ttaaaagatg acccaagcca aagcgctaac cttttagcag aagctaaaaa
      841 gctaaatgat gctcaggcgc cgaaagtaga cgcgaatcat cagctgcagg tcgacctcga
      901 gtctagggga tccccgggga tcccgaattc ggctcgtact aagcagaccg ctcgcaagtc
      961 caccggcggc aaggccccgc gcaagcagct ggcccagatc ccgaattcgg ctcgtactaa
     1021 gcagaccgct cgcaagtcca ccggcggcaa ggccccgcgc aagcagctgg cccagatccc
     1081 gaattcggct cgtactaagc agaccgctcg caagtccacc ggcggcaagg ccccgcgcaa
     1141 gcagctggcc cagatcccga attcggctcg tactaagcag accgctcgca agtccaccgg
     1201 cggcaaggcc ccgcgcaagc agctggccca gatctgcgga cgcatctttt acccatacga
     1261 tgttcctgac tatgcgggct atccctatga cgtcccggac tatgcaggat cctatccata
     1321 tgacgttcca gattacgctg ctcagtgcgg acgctagggt acccagcttt tgtggcgcgc
     1381 ccgacctcga gtcatgtaat tagttatgtc acgcttacat tcacgccctc cccccacatc
     1441 cgctctaacc gaaaaggaag gagttagaca acctgaagtc taggtcccta tttatttttt
     1501 tatagttatg ttagtattaa gaacgttatt tatatttcaa atttttcttt tttttctgta
     1561 cagacgcgtg tacgcatgta acattatact gaaaaccttg cttgagaagg ttttgggacg
     1621 ctcgaaggct ttaatttgcg gccggtacta gtcaggtcga cgcggcatca gagcagattg
     1681 tactgagagt gcaccatacc accttttcaa ttcatcattt tttttttatt cttttttttg
     1741 atttcggttt ccttgaaatt tttttgattc ggtaatctcc gaacagaagg aagaacgaag
     1801 gaaggagcac agacttagat tggtatatat acgcatatgt agtgttgaag aaacatgaaa
     1861 ttgcccagta ttcttaaccc aactgcacag aacaaaaaca tgcaggaaac gaagataaat
     1921 catgtcgaaa gctacatata aggaacgtgc tgctactcat cctagtcctg ttgctgccaa
     1981 gctatttaat atcatgcacg aaaagcaaac aaacttgtgt gcttcattgg atgttcgtac
     2041 caccaaggaa ttactggagt tagttgaagc attaggtccc aaaatttgtt tactaaaaac
     2101 acatgtggat atcttgactg atttttccat ggagggcaca gttaagccgc taaaggcatt
     2161 atccgccaag tacaattttt tactcttcga agacagaaaa tttgctgaca ttggtaatac
     2221 agtcaaattg cagtactctg cgggtgtata cagaatagca gaatgggcag acattacgaa
     2281 tgcacacggt gtggtgggcc caggtattgt tagcggtttg aagcaggcgg cagaagaagt
     2341 aacaaaggaa cctagaggcc ttttgatgtt agcagaattg tcatgcaagg gctccctatc
     2401 tactggagaa tatactaagg gtactgttga cattgcgaag agcgacaaag attttgttat
     2461 cggctttatt gctcaaagag acatgggtgg aagagatgaa ggttacgatt ggttgattat
     2521 gacacccggt gtgggtttag atgacaaggg agacgcattg ggtcaacagt atagaaccgt
     2581 ggatgatgtg gtctctacag gatctgacat tattattgtt ggaagaggac tatttgcaaa
     2641 gggaagggat gctaaggtag agggtgaacg ttacagaaaa gcaggctggg aagcatattt
     2701 gagaagatgc ggccagcaaa actaaaaaac tgtattataa gtaaatgcat gtatactaaa
     2761 ctcacaaatt agagcttcaa tttaattata tcagttatta ccctatgcgg tgtgaaatac
     2821 cgcacagatg cggcatgccc tgcccctaat cagtactgac aataaaaaga ttcttgtttt
     2881 caagaacttg tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt
     2941 tagcgtgatt tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc
     3001 gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat
     3061 tcgatactaa cgccgccatc cagtttaaac gagctccaat tcgccctata gtgagtcgta
     3121 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     3181 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     3241 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga aattgtaagc
     3301 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     3361 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     3421 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     3481 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     3541 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     3601 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     3661 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     3721 cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     3781 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     3841 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     3901 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     3961 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     4021 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     4081 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     4141 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     4201 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     4261 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     4321 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     4381 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     4441 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     4501 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     4561 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     4621 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     4681 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     4741 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     4801 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     4861 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4921 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4981 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     5041 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     5101 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     5161 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     5221 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     5281 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     5341 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     5401 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     5461 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     5521 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     5581 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     5641 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     5701 ctccccgcgc
//
