LOCUS       40924_10871        5233 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCK901, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Kraft C.
  TITLE     A toolbox for the analysis of weak and low abundant molecule 
            interactions in vivo by M-Track in budding yeast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5233)
  AUTHORS   Brezovich A, Schuschnig M, Ammerer G, Kraft C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2014) Department of Biochemistry and Cell Biology,
            MFPL, University of Vienna, Dr. Bohr Gasse 9, Vienna 1030, Austria
REFERENCE   3  (bases 1 to 5233)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5233)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2014) Department of Biochemistry and Cell Biology, MFPL, 
            University of Vienna, Dr. Bohr Gasse 9, Vienna 1030, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    78..99
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        114..144
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    152..168
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     176..192
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        213..231
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    265..311
                     /label=GFP region
                     /note="GFP region"
     CDS             318..344
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     CDS             351..377
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     misc_feature    459..752
                     /label=4x Histone3
                     /note="4x Histone3"
     CDS             777..866
                     /codon_start=1
                     /label=3xHA
                     /note="three tandem HA epitope tags"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      919..1166
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        1233..1448
                     /label=URA3 promoter
     CDS             1449..2249
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     terminator      2382..2579
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(2632..2650)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2660..2676)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            2748..2816
                     /gene="LacZ alpha"
                     /label=LacZ alpha
     rep_origin      complement(2817..3272)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3299..3403
                     /label=AmpR promoter
     CDS             3404..4261
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4435..5023
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
       61 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
      121 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
      181 aacagctatg accatgatta cgccaagctc gaaattaacc ctcactaaag ggaacaaaag
      241 ctgatcgaat tcctgcagcc cgggggatcc ccgggttaat taacagtaaa ggagaagaac
      301 ttttcactgg actcgagtat ccgtatgacg tcccggacta tgcaggatcc tatccatatg
      361 acgttccaga ttacgctctg caggaattcg atatcaagct tgcggccgcc ctgcaggtcg
      421 acctcgagtc taggggatcc ccggggatcc cgaattcggc tcgtactaag cagaccgctc
      481 gcaagtccac cggcggcaag gccccgcgca agcagctggc ccagatcccg aattcggctc
      541 gtactaagca gaccgctcgc aagtccaccg gcggcaaggc cccgcgcaag cagctggccc
      601 agatcccgaa ttcggctcgt actaagcaga ccgctcgcaa gtccaccggc ggcaaggccc
      661 cgcgcaagca gctggcccag atcccgaatt cggctcgtac taagcagacc gctcgcaagt
      721 ccaccggcgg caaggccccg cgcaagcagc tggcccagat ctgcggacgc atcttttacc
      781 catacgatgt tcctgactat gcgggctatc cctatgacgt cccggactat gcaggatcct
      841 atccatatga cgttccagat tacgctgctc agtgcggacg ctagggtacc cagcttttgt
      901 ggcgcgcccg acctcgagtc atgtaattag ttatgtcacg cttacattca cgccctcccc
      961 ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt
     1021 atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt
     1081 ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt
     1141 tgggacgctc gaaggcttta atttgcggcc ggtactagtc aggtcgacgc ggcatcagag
     1201 cagattgtac tgagagtgca ccataccacc ttttcaattc atcatttttt ttttattctt
     1261 ttttttgatt tcggtttcct tgaaattttt ttgattcggt aatctccgaa cagaaggaag
     1321 aacgaaggaa ggagcacaga cttagattgg tatatatacg catatgtagt gttgaagaaa
     1381 catgaaattg cccagtattc ttaacccaac tgcacagaac aaaaacatgc aggaaacgaa
     1441 gataaatcat gtcgaaagct acatataagg aacgtgctgc tactcatcct agtcctgttg
     1501 ctgccaagct atttaatatc atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg
     1561 ttcgtaccac caaggaatta ctggagttag ttgaagcatt aggtcccaaa atttgtttac
     1621 taaaaacaca tgtggatatc ttgactgatt tttccatgga gggcacagtt aagccgctaa
     1681 aggcattatc cgccaagtac aattttttac tcttcgaaga cagaaaattt gctgacattg
     1741 gtaatacagt caaattgcag tactctgcgg gtgtatacag aatagcagaa tgggcagaca
     1801 ttacgaatgc acacggtgtg gtgggcccag gtattgttag cggtttgaag caggcggcag
     1861 aagaagtaac aaaggaacct agaggccttt tgatgttagc agaattgtca tgcaagggct
     1921 ccctatctac tggagaatat actaagggta ctgttgacat tgcgaagagc gacaaagatt
     1981 ttgttatcgg ctttattgct caaagagaca tgggtggaag agatgaaggt tacgattggt
     2041 tgattatgac acccggtgtg ggtttagatg acaagggaga cgcattgggt caacagtata
     2101 gaaccgtgga tgatgtggtc tctacaggat ctgacattat tattgttgga agaggactat
     2161 ttgcaaaggg aagggatgct aaggtagagg gtgaacgtta cagaaaagca ggctgggaag
     2221 catatttgag aagatgcggc cagcaaaact aaaaaactgt attataagta aatgcatgta
     2281 tactaaactc acaaattaga gcttcaattt aattatatca gttattaccc tatgcggtgt
     2341 gaaataccgc acagatgcgg catgccctgc ccctaatcag tactgacaat aaaaagattc
     2401 ttgttttcaa gaacttgtca tttgtatagt ttttttatat tgtagttgtt ctattttaat
     2461 caaatgttag cgtgatttat attttttttc gcctcgacat catctgccca gatgcgaagt
     2521 taagtgcgca gaaagtaata tcatgcgtca atcgtatgtg aatgctggtc gctatactgc
     2581 tgtcgattcg atactaacgc cgccatccag tttaaacgag ctccaattcg ccctatagtg
     2641 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     2701 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     2761 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggaaat
     2821 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     2881 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     2941 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     3001 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     3061 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3121 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3181 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3241 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     3301 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3361 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     3421 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     3481 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     3541 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     3601 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     3661 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     3721 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     3781 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     3841 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     3901 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     3961 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     4021 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     4081 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     4141 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     4201 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     4261 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     4321 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     4381 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4441 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4501 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4561 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     4621 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4681 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4741 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4801 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4861 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4921 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4981 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5041 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     5101 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     5161 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     5221 accgcctctc ccc
//
