LOCUS       40924_10866        6653 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCK900, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   Kraft C.
  TITLE     A toolbox for the analysis of weak and low abundant molecule 
            interactions in vivo by M-Track in budding yeast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6653)
  AUTHORS   Brezovich A, Schuschnig M, Ammerer G, Kraft C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2014) Department of Biochemistry and Cell Biology,
            MFPL, University of Vienna, Dr. Bohr Gasse 9, Vienna 1030, Austria
REFERENCE   3  (bases 1 to 6653)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6653)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2014) Department of Biochemistry and Cell Biology, MFPL, 
            University of Vienna, Dr. Bohr Gasse 9, Vienna 1030, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    31..52
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        67..97
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..121
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     129..145
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        166..184
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    218..264
                     /label=GFP region
                     /note="GFP region"
     CDS             304..333
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             340..369
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             376..405
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             424..453
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             460..489
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             496..525
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             544..573
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             580..609
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             616..645
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    679..1671
                     /label=HKMT
                     /note="HKMT"
     terminator      1718..1965
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        2221..2625
                     /label=LEU2 promoter
     CDS             2638..3729
                     /codon_start=1
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     terminator      3755..3952
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(4005..4023)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4033..4049)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            4121..4189
                     /gene="LacZ alpha"
                     /label=LacZ alpha
     rep_origin      complement(4190..4645)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4672..4776
                     /label=AmpR promoter
     CDS             4777..5634
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5808..6396
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
       61 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
      121 atttcacaca ggaaacagct atgaccatga ttacgccaag ctcgaaatta accctcacta
      181 aagggaacaa aagctgatcg aattcctgca gcccggggga tccccgggtt aattaacagt
      241 aaaggagaag aacttttcac tggactcgag gcggccgccc tgcagggttc tgctgctagt
      301 ggtgaacaaa agttgatttc tgaagaagat ttgaacggtg aacaaaagct aatctccgag
      361 gaagacttga acggtgaaca aaaattaatc tcagaagaag acttgaacgg atcctctaga
      421 ggtgaacaaa agttgatttc tgaagaagat ttgaacggtg aacaaaagct aatctccgag
      481 gaagacttga acggtgaaca aaaattaatc tcagaagaag acttgaacgg atcctctaga
      541 ggtgaacaaa agttgatttc tgaagaagat ttgaacggtg aacaaaagct aatctccgag
      601 gaagacttga acggtgaaca aaaattaatc tcagaagaag acttgaacgg atccactctg
      661 caggagtcta ggggatcctg tgtgcgtatc ctcaagcagt tccacaagga cttagaaagg
      721 gagctgctcc ggcggcacca ccggtcaaag accccccggc acctggaccc aagcttggcc
      781 aactacctgg tgcagaaggc caagcagagg cgggcgctcc gtcgctggga gcaggagctc
      841 aatgccaagc gcagccatct gggacgcatc actgtagaga atgaggtgga cctggacggc
      901 cctcctcggg ccttcgtgta catcaatgag taccgtgttg gtgagggcat caccctcaac
      961 caggtggctg tgggctgcga gtgccaggac tgtctgtggg cacccactgg aggctgctgc
     1021 ccgggggcgt cactgcacaa gtttgcctac aatgaccagg gccaggtgcg gcttcgagcc
     1081 gggctgccca tctacgagtg caactcccgc tgccgctgcg gctatgactg cccaaatcgt
     1141 gtggtacaga agggtatccg atatgacctc tgcatcttcc gcacggatga tgggcgtggc
     1201 tggggcgtcc gcaccctgga gaagattcgc aagaacagct tcgtcatgga gtacgtggga
     1261 gagatcatta cctcagagga ggcagagcgg cggggccaga tctacgaccg tcagggcgcc
     1321 acctacctct ttgacctgga ctacgtggag gacgtgtaca ccgtggatgc cgcctactat
     1381 ggcaacatct cccgctttgt caaccacagt tgtgacccca acctacaggt gtacaacgtc
     1441 ttcatagaca accttgacga gcggctgccc cgcatcgctt tctttgccac aagaaccatc
     1501 cgggcaggcg aggagctcac ctttgattac aacatgcaag tggaccccgt ggacatggag
     1561 agcacccgca tggactccaa ctttggcctg gctgggctcc ctggctcccc taagaagcgg
     1621 gtccgtattg aatgcaagtg tgggactgag tcctgccgca aatacctctt ctaggtcgac
     1681 tagggtaccc agcttttgtg gcgcgcccga cctcgagtca tgtaattagt tatgtcacgc
     1741 ttacattcac gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct
     1801 gaagtctagg tccctattta tttttttata gttatgttag tattaagaac gttatttata
     1861 tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa
     1921 accttgcttg agaaggtttt gggacgctcg aaggctttaa tttgcggccg gtactagtcc
     1981 tcgaggagaa cttctagtat atctacatac ctaatattat tgccttatta aaaatggaat
     2041 cccaacaatt acatcaaaat ccacattctc ttcaaaatca attgtcctgt acttccttgt
     2101 tcatgtgtgt tcaaaaacgt tatatttata ggataattat actctatttc tcaacaagta
     2161 attggttgtt tggccgagcg gtctaaggcg cctgattcaa gaaatatctt gaccgcagtt
     2221 aactgtggga atactcaggt atcgtaagat gcaagagttc gaatctctta gcaaccatta
     2281 tttttttcct caacataacg agaacacaca ggggcgctat cgcacagaat caaattcgat
     2341 gactggaaat tttttgttaa tttcagaggt cgcctgacgc atataccttt ttcaactgaa
     2401 aaattgggag aaaaaggaaa ggtgagaggc cggaaccggc ttttcatata gaatagagaa
     2461 gcgttcatga ctaaatgctt gcatcacaat acttgaagtt gacaatatta tttaaggacc
     2521 tattgttttt tccaataggt ggttagcaat cgtcttactt tctaactttt cttacctttt
     2581 acatttcagc aatatatata tatatttcaa ggatatacca ttctaatgtc tgcccctatg
     2641 tctgccccta agaagatcgt cgttttgcca ggtgaccacg ttggtcaaga aatcacagcc
     2701 gaagccatta aggttcttaa agctatttct gatgttcgtt ccaatgtcaa gttcgatttc
     2761 gaaaatcatt taattggtgg tgctgctatc gatgctacag gtgtcccact tccagatgag
     2821 gcgctggaag cctccaagaa ggctgatgcc gttttgttag gtgctgtggg tggtcctaaa
     2881 tggggtaccg gtagtgttag acctgaacaa ggtttactaa aaatccgtaa agaacttcaa
     2941 ttgtacgcca acttaagacc atgtaacttt gcatccgact ctcttttaga cttatctcca
     3001 atcaagccac aatttgctaa aggtactgac ttcgttgttg tcagagaatt agtgggaggt
     3061 atttactttg gtaagagaaa ggaagacgat ggtgatggtg tcgcttggga tagtgaacaa
     3121 tacaccgttc cagaagtgca aagaatcaca agaatggccg ctttcatggc cctacaacat
     3181 gagccaccat tgcctatttg gtccttggat aaagctaatg ttttggcctc ttcaagatta
     3241 tggagaaaaa ctgtggagga aaccatcaag aacgaattcc ctacattgaa ggttcaacat
     3301 caattgattg attctgccgc catgatccta gttaagaacc caacccacct aaatggtatt
     3361 ataatcacca gcaacatgtt tggtgatatc atctccgatg aagcctccgt tatcccaggt
     3421 tccttgggtt tgttgccatc tgcgtccttg gcctctttgc cagacaagaa caccgcattt
     3481 ggtttgtacg aaccatgcca cggttctgct ccagatttgc caaagaataa ggttgaccct
     3541 atcgccacta tcttgtctgc tgcaatgatg ttgaaattgt cattgaactt gcctgaagaa
     3601 ggtaaggcca ttgaagatgc agttaaaaag gttttggatg caggtatcag aactggtgat
     3661 ttaggtggtt ccaacagtac caccgaagtc ggtgatgctg tcgccgaaga agttaagaaa
     3721 atccttgctt aagcatgccc tgcccctaat cagtactgac aataaaaaga ttcttgtttt
     3781 caagaacttg tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt
     3841 tagcgtgatt tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc
     3901 gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat
     3961 tcgatactaa cgccgccatc cagtttaaac gagctccaat tcgccctata gtgagtcgta
     4021 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     4081 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     4141 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga aattgtaagc
     4201 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     4261 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     4321 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     4381 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     4441 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     4501 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     4561 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     4621 cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4681 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4741 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     4801 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     4861 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     4921 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     4981 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5041 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5101 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5161 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5221 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5281 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5341 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5401 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5461 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5521 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5581 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5641 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     5701 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     5761 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     5821 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     5881 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     5941 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6001 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6061 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6121 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6181 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6241 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6301 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6361 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6421 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     6481 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     6541 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     6601 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cga
//
