LOCUS       40924_10861        5383 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCK9, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Kemmer C, Gitzinger M, Daoud-El Baba M, Djonov V, Stelling J, 
            Fussenegger M.
  TITLE     Self-sufficient control of urate homeostasis in mice by a synthetic 
            circuit
  JOURNAL   Nat. Biotechnol. 28 (4), 355-360 (2010)
  PUBMED    20351688
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Kemmer C, Gitzinger M, Daoud-El Baba M, Djonov V, Stelling J, 
            Fussenegger M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2010) Department of Biosystems Science and 
            Engineering, D-BSSE, ETH Zurich, Mattenstrasse 20, Basel 4058, 
            Switzerland
REFERENCE   3  (bases 1 to 5383)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5383)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Biotechnol."; date: "2010"; volume: "28"; issue: "4"; pages: 
            "355-360"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-NOV-2010) Department of Biosystems Science and Engineering, 
            D-BSSE, ETH Zurich, Mattenstrasse 20, Basel 4058, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        48..244
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     repeat_region   258..519
                     /label=synthetic octameric hucO repeat (hucO8)
                     /note="synthetic octameric hucO repeat (hucO8)"
     CDS             534..2042
                     /label=SEAP
                     /note="secreted alkaline phosphatase from human placenta"
     polyA_signal    complement(2100..2221)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      2324..2569
                     /label=SV40 enhancer
                     /note="SV40 enhancer"
                     /regulatory_class="enhancer"
     rep_origin      complement(2886..3474)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3648..4505)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4506..4610)
                     /label=AmpR promoter
     rep_origin      4637..5092
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5223..5271
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5285..5376
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta
       61 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
      121 cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc
      181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
      241 caaaaagctt agacgtcgcc ctcagtaggt agacatctaa gtatctggcg ccctcagtag
      301 gtagacatct aagtatctgg cgccctcagt aggtagacat ctaagtatct ggcgccctca
      361 gtaggtagac atctaagtat ctggcgatat cgccctcagt aggtagacat ctaagtatct
      421 ggcgccctca gtaggtagac atctaagtat ctggcgccct cagtaggtag acatctaagt
      481 atctggcgcc ctcagtaggt agacatctaa gtatctggcc ctgcaggaat tcgcccacca
      541 tgctgctgct gctgctgctg ctgggcctga ggctacagct ctccctgggc atcatcccag
      601 ttgaggagga gaacccggac ttctggaacc gcgaggcagc cgaggccctg ggtgccgcca
      661 agaagctgca gcctgcacag acagccgcca agaacctcat catcttcctg ggcgatggga
      721 tgggggtgtc tacggtgaca gctgccagga tcctaaaagg gcagaagaag gacaaactgg
      781 ggcctgagat acccctggcc atggaccgct tcccatatgt ggctctgtcc aagacataca
      841 atgtagacaa acatgtgcca gacagtggag ccacagccac ggcctacctg tgcggggtca
      901 agggcaactt ccagaccatt ggcttgagtg cagccgcccg ctttaaccag tgcaacacga
      961 cacgcggcaa cgaggtcatc tccgtgatga atcgggccaa gaaagcaggg aagtcagtgg
     1021 gagtggtaac caccacacga gtgcagcacg cctcgccagc cggcacctac gcccacacgg
     1081 tgaaccgcaa ctggtactcg gacgccgacg tgcctgcctc ggcccgccag gaggggtgcc
     1141 aggacatcgc tacgcagctc atctccaaca tggacattga cgtgatccta ggtggaggcc
     1201 gaaagtacat gtttcgcatg ggaaccccag accctgagta cccagatgac tacagccaag
     1261 gtgggaccag gctggacggg aagaatctgg tgcaggaatg gctggcgaag cgccagggtg
     1321 cccggtatgt gtggaaccgc actgagctca tgcaggcttc cctggacccg tctgtgaccc
     1381 atctcatggg tctctttgag cctggagaca tgaaatacga gatccaccga gactccacac
     1441 tggacccctc cctgatggag atgacagagg ctgccctgcg cctgctgagc aggaaccccc
     1501 gcggcttctt cctcttcgtg gagggtggtc gcatcgacca tggtcatcat gaaagcaggg
     1561 cttaccgggc actgactgag acgatcatgt tcgacgacgc cattgagagg gcgggccagc
     1621 tcaccagcga ggaggacacg ctgagcctcg tcactgccga ccactcccac gtcttctcct
     1681 tcggaggcta ccccctgcga gggagctcca tcttcgggct ggcccctggc aaggcccggg
     1741 acaggaaggc ctacacggtc ctcctatacg gaaacggtcc aggctatgtg ctcaaggacg
     1801 gcgcccggcc ggatgttacc gagagcgaga gcgggagccc cgagtatcgg cagcagtcag
     1861 cagtgcccct ggacgaagag acccacgcag gcgaggacgt ggcggtgttc gcgcgcggcc
     1921 cgcaggcgca cctggttcac ggcgtgcagg agcagacctt catagcgcac gtcatggcct
     1981 tcgccgcctg cctggagccc tacaccgcct gcgacctggc gccccccgcc ggcaccaccg
     2041 acgccgcgca cccgggttac tctagagtcg gggcggccgg ccgcttcgag cagacatgat
     2101 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     2161 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     2221 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     2281 ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatctga acgatggagc
     2341 ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact
     2401 atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
     2461 gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     2521 ggggagcctg gggactttcc acaccctaac tgacacacat tccacagcgg atccgtcgac
     2581 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
     2641 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
     2701 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     2761 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     2821 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2881 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2941 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3001 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3061 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3121 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3181 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3241 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3301 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     3361 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3421 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3481 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3541 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3601 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     3661 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     3721 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     3781 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     3841 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3901 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3961 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     4021 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4081 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     4141 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     4201 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4261 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4321 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4381 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4441 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4501 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     4561 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4621 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4681 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4741 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4801 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     4861 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4921 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     4981 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     5041 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcccattcg
     5101 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     5161 cagcccaagc taccatgata agtaagtaat attaaggtac gggaggtact tggagcggcc
     5221 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag
     5281 tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg
     5341 tccccagtgc aagtgcaggt gccagaacat ttctctatcg ata
//
