LOCUS       40924_10856        5768 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCK73, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Kemmer C, Gitzinger M, Daoud-El Baba M, Djonov V, Stelling J, 
            Fussenegger M.
  TITLE     Self-sufficient control of urate homeostasis in mice by a synthetic 
            circuit
  JOURNAL   Nat. Biotechnol. 28 (4), 355-360 (2010)
  PUBMED    20351688
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Kemmer C, Gitzinger M, Daoud-El Baba M, Djonov V, Stelling J, 
            Fussenegger M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2010) Department of Biosystems Science and 
            Engineering, D-BSSE, ETH Zurich, Mattenstrasse 20, Basel 4058, 
            Switzerland
REFERENCE   3  (bases 1 to 5768)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5768)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Biotechnol."; date: "2010"; volume: "28"; issue: "4"; pages: 
            "355-360"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-NOV-2010) Department of Biosystems Science and Engineering, 
            D-BSSE, ETH Zurich, Mattenstrasse 20, Basel 4058, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        52..431
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        432..634
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     repeat_region   643..904
                     /label=synthetic octameric hucO repeat (hucO8)
                     /note="synthetic octameric hucO repeat (hucO8)"
     CDS             919..2427
                     /label=SEAP
                     /note="secreted alkaline phosphatase from human placenta"
     polyA_signal    complement(2485..2606)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      2709..2954
                     /label=simian virus 40 enhancer
                     /note="simian virus 40 enhancer"
                     /regulatory_class="enhancer"
     rep_origin      complement(3271..3859)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4033..4890)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4891..4995)
                     /label=AmpR promoter
     rep_origin      5022..5477
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5608..5656
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5670..5761
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgaggaa gatctcgcgt tgacattgat
       61 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
      121 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      181 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      241 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      301 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      361 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      421 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      481 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      541 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      601 ggcgtgtacg gtgggaggtc tatataagca agcttagacg tcgccctcag taggtagaca
      661 tctaagtatc tggcgccctc agtaggtaga catctaagta tctggcgccc tcagtaggta
      721 gacatctaag tatctggcgc cctcagtagg tagacatcta agtatctggc gatatcgccc
      781 tcagtaggta gacatctaag tatctggcgc cctcagtagg tagacatcta agtatctggc
      841 gccctcagta ggtagacatc taagtatctg gcgccctcag taggtagaca tctaagtatc
      901 tggccctgca ggaattcgcc caccatgctg ctgctgctgc tgctgctggg cctgaggcta
      961 cagctctccc tgggcatcat cccagttgag gaggagaacc cggacttctg gaaccgcgag
     1021 gcagccgagg ccctgggtgc cgccaagaag ctgcagcctg cacagacagc cgccaagaac
     1081 ctcatcatct tcctgggcga tgggatgggg gtgtctacgg tgacagctgc caggatccta
     1141 aaagggcaga agaaggacaa actggggcct gagatacccc tggccatgga ccgcttccca
     1201 tatgtggctc tgtccaagac atacaatgta gacaaacatg tgccagacag tggagccaca
     1261 gccacggcct acctgtgcgg ggtcaagggc aacttccaga ccattggctt gagtgcagcc
     1321 gcccgcttta accagtgcaa cacgacacgc ggcaacgagg tcatctccgt gatgaatcgg
     1381 gccaagaaag cagggaagtc agtgggagtg gtaaccacca cacgagtgca gcacgcctcg
     1441 ccagccggca cctacgccca cacggtgaac cgcaactggt actcggacgc cgacgtgcct
     1501 gcctcggccc gccaggaggg gtgccaggac atcgctacgc agctcatctc caacatggac
     1561 attgacgtga tcctaggtgg aggccgaaag tacatgtttc gcatgggaac cccagaccct
     1621 gagtacccag atgactacag ccaaggtggg accaggctgg acgggaagaa tctggtgcag
     1681 gaatggctgg cgaagcgcca gggtgcccgg tatgtgtgga accgcactga gctcatgcag
     1741 gcttccctgg acccgtctgt gacccatctc atgggtctct ttgagcctgg agacatgaaa
     1801 tacgagatcc accgagactc cacactggac ccctccctga tggagatgac agaggctgcc
     1861 ctgcgcctgc tgagcaggaa cccccgcggc ttcttcctct tcgtggaggg tggtcgcatc
     1921 gaccatggtc atcatgaaag cagggcttac cgggcactga ctgagacgat catgttcgac
     1981 gacgccattg agagggcggg ccagctcacc agcgaggagg acacgctgag cctcgtcact
     2041 gccgaccact cccacgtctt ctccttcgga ggctaccccc tgcgagggag ctccatcttc
     2101 gggctggccc ctggcaaggc ccgggacagg aaggcctaca cggtcctcct atacggaaac
     2161 ggtccaggct atgtgctcaa ggacggcgcc cggccggatg ttaccgagag cgagagcggg
     2221 agccccgagt atcggcagca gtcagcagtg cccctggacg aagagaccca cgcaggcgag
     2281 gacgtggcgg tgttcgcgcg cggcccgcag gcgcacctgg ttcacggcgt gcaggagcag
     2341 accttcatag cgcacgtcat ggccttcgcc gcctgcctgg agccctacac cgcctgcgac
     2401 ctggcgcccc ccgccggcac caccgacgcc gcgcacccgg gttactctag agtcggggcg
     2461 gccggccgct tcgagcagac atgataagat acattgatga gtttggacaa accacaacta
     2521 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     2581 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     2641 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa
     2701 tcgataagga tctgaacgat ggagcggaga atgggcggaa ctgggcggag ttaggggcgg
     2761 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
     2821 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
     2881 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
     2941 cacattccac agcggatccg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc
     3001 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg
     3061 caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg actcgctgcg
     3121 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3181 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3241 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3301 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3361 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3421 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3481 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3541 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3601 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3661 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     3721 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3781 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3841 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3901 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3961 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     4021 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     4081 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     4141 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     4201 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     4261 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     4321 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     4381 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     4441 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     4501 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     4561 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     4621 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     4681 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     4741 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     4801 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     4861 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     4921 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     4981 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc
     5041 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     5101 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     5161 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5221 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     5281 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     5341 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     5401 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     5461 attaacgttt acaatttccc attcgccatt caggctgcgc aactgttggg aagggcgatc
     5521 ggtgcgggcc tcttcgctat tacgccagcc caagctacca tgataagtaa gtaatattaa
     5581 ggtacgggag gtacttggag cggccgcaat aaaatatctt tattttcatt acatctgtgt
     5641 gttggttttt tgtgtgaatc gatagtacta acatacgctc tccatcaaaa caaaacgaaa
     5701 caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg caggtgccag aacatttctc
     5761 tatcgata
//
