LOCUS       V008489                 4828 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008489
VERSION     V008489
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Kemmer C, Gitzinger M, Daoud-El Baba M, Djonov V, Stelling J,
            Fussenegger M.
  TITLE     Self-sufficient control of urate homeostasis in mice by a synthetic
            circuit
  JOURNAL   Nat. Biotechnol. 28 (4), 355-360 (2010)
   PUBMED   20351688
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Kemmer C, Gitzinger M, Daoud-El Baba M, Djonov V, Stelling J,
            Fussenegger M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2010) Department of Biosystems Science and
            Engineering, D-BSSE, ETH Zurich, Mattenstrasse 20, Basel 4058,
            Switzerland
REFERENCE   3  (bases 1 to 4828)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4828)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat.
            Biotechnol."; date: "2010"; volume: "28"; issue: "4"; pages:
            "355-360"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-NOV-2010) Department of Biosystems Science and Engineering,
            D-BSSE, ETH Zurich, Mattenstrasse 20, Basel 4058, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        48..244
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     repeat_region   258..519
                     /label="synthetic octameric hucO repeat (hucO8)"
                     /note="synthetic octameric hucO repeat (hucO8)"
     sig_peptide     537..599
                     /label="Ig-kappa leader"
                     /note="leader sequence from mouse immunoglobulin kappa
                     light chain"
     CDS             600..1502
                     /gene="uaZ"
                     /label="Uricase"
                     /note="Uricase from Aspergillus flavus. Accession#: Q00511"
     polyA_signal    complement(1545..1666)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     regulatory      1769..2014
                     /label="simian virus 40 enhancer"
                     /note="simian virus 40 enhancer"
                     /regulatory_class="enhancer"
     rep_origin      complement(2331..2919)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3093..3950)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3951..4055)
                     /label="AmpR promoter"
     rep_origin      4082..4537
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4668..4716
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    4730..4821
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta
       61 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
      121 cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc
      181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
      241 caaaaagctt agacgtcgcc ctcagtaggt agacatctaa gtatctggcg ccctcagtag
      301 gtagacatct aagtatctgg cgccctcagt aggtagacat ctaagtatct ggcgccctca
      361 gtaggtagac atctaagtat ctggcgatat cgccctcagt aggtagacat ctaagtatct
      421 ggcgccctca gtaggtagac atctaagtat ctggcgccct cagtaggtag acatctaagt
      481 atctggcgcc ctcagtaggt agacatctaa gtatctggcc ctgcaggaat tccaccatgg
      541 agaccgatac actgctgctg tgggtgctgc tcctgtgggt gccaggaagt acaggagatt
      601 ctgctgtgaa ggccgcaaga tatggcaagg ataatgtgag ggtgtacaag gtgcataagg
      661 acgaaaagac tggcgtgcag acagtgtacg agatgaccgt gtgcgtcctg ctggagggcg
      721 aaatcgagac ttcttatacc aaagccgaca actccgtgat tgtggccaca gattctatca
      781 agaacactat ctatatcacc gccaaacaga acccagtgac accacctgaa ctgttcggca
      841 gcattctcgg cacacacttt attgagaagt acaaccacat ccatgctgca cacgtgaata
      901 tcgtgtgtca tcgctggacc cgcatggaca tcgacggaaa gccacacccc cactctttta
      961 tcagagactc tgaagaaaag agaaacgtgc aggtcgacgt ggtggaggga aaaggtatcg
     1021 acatcaagag ctcactctcc ggcctgaccg tgctgaagag taccaattca cagttttggg
     1081 ggtttctgag agacgaatac actacactga aggagacttg ggatagaatc ctgagtaccg
     1141 acgtggatgc aacctggcag tggaagaatt tttccgggct gcaggaagtg cggtcccacg
     1201 tgcccaagtt tgatgcaacc tgggcaaccg caagggaggt gacactgaaa acctttgccg
     1261 aggacaactc cgctagcgtg caggccacaa tgtacaagat ggccgaacag atcctggcca
     1321 gacagcagct gattgagact gtggagtact ctctgcctaa caagcactat ttcgaaatcg
     1381 acctgtcctg gcacaaggga ctgcagaata ctggtaaaaa cgcagaggtg ttcgcccctc
     1441 agagtgatcc caatggtctg atcaaatgca cagtggggag atcctctctg aagagcaagc
     1501 tgtaatctag agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga
     1561 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     1621 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     1681 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     1741 cctctacaaa tgtggtaaaa tcgataagga tctgaacgat ggagcggaga atgggcggaa
     1801 ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa
     1861 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg
     1921 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     1981 tttccacacc ctaactgaca cacattccac agcggatccg tcgaccgatg cccttgagag
     2041 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     2101 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc
     2161 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2221 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2281 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2341 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2401 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2461 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2521 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2581 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2641 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2701 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2761 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2821 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2881 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2941 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3001 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3061 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3121 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3181 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3241 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3301 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3361 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3421 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3481 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3541 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3601 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3661 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     3721 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     3781 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     3841 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     3901 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     3961 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4021 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     4081 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4141 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4201 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4261 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4321 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4381 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     4441 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     4501 aacgcgaatt ttaacaaaat attaacgttt acaatttccc attcgccatt caggctgcgc
     4561 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc caagctacca
     4621 tgataagtaa gtaatattaa ggtacgggag gtacttggag cggccgcaat aaaatatctt
     4681 tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc
     4741 tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg
     4801 caggtgccag aacatttctc tatcgata
//
