LOCUS       40924_10846        4867 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCK53, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Kemmer C, Fluri DA, Witschi U, Passeraub A, Gutzwiller A, 
            Fussenegger M.
  TITLE     A designer network coordinating bovine artificial insemination by 
            ovulation-triggered release of implanted sperms
  JOURNAL   J Control Release 150 (1), 23-29 (2011)
  PUBMED    21108977
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Kemmer C, Fluri DA, Witschi U, Passeraub A, Gutzwiller A, 
            Fussenegger M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2013) BSSE, ETH Zurich, Mattenstrasse 26, Basel, 
            BS 4058, Switzerland
REFERENCE   3  (bases 1 to 4867)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4867)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Control 
            Release"; date: "2011"; volume: "150"; issue: "1"; pages: "23-29"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-AUG-2013) BSSE, ETH Zurich, Mattenstrasse 26, Basel, BS 4058, 
            Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3..1505
                     /label=SEAP
                     /note="secreted alkaline phosphatase from human placenta"
     polyA_signal    complement(1563..1684)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2103..2691)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2865..3722)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3723..3827)
                     /label=AmpR promoter
     rep_origin      3854..4309
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4440..4488
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4502..4593
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     regulatory      4627..4866
                     /label=CRE promoter
                     /note="CRE promoter"
                     /regulatory_class="promoter"
ORIGIN
        1 gaatgctgct gctgctgctg ctgctgggcc tgaggctaca gctctccctg ggcatcatcc
       61 cagttgagga ggagaacccg gacttctgga accgcgaggc agccgaggcc ctgggtgccg
      121 ccaagaagct gcagcctgca cagacagccg ccaagaacct catcatcttc ctgggcgatg
      181 ggatgggggt gtctacggtg acagctgcca ggatcctaaa agggcagaag aaggacaaac
      241 tggggcctga gatacccctg gccatggacc gcttcccata tgtggctctg tccaagacat
      301 acaatgtaga caaacatgtg ccagacagtg gagccacagc cacggcctac ctgtgcgggg
      361 tcaagggcaa cttccagacc attggcttga gtgcagccgc ccgctttaac cagtgcaaca
      421 cgacacgcgg caacgaggtc atctccgtga tgaatcgggc caagaaagca gggaagtcag
      481 tgggagtggt aaccaccaca cgagtgcagc acgcctcgcc agccggcacc tacgcccaca
      541 cggtgaaccg caactggtac tcggacgccg acgtgcctgc ctcggcccgc caggaggggt
      601 gccaggacat cgctacgcag ctcatctcca acatggacat tgacgtgatc ctaggtggag
      661 gccgaaagta catgtttcgc atgggaaccc cagaccctga gtacccagat gactacagcc
      721 aaggtgggac caggctggac gggaagaatc tggtgcagga atggctggcg aagcgccagg
      781 gtgcccggta tgtgtggaac cgcactgagc tcatgcaggc ttccctggac ccgtctgtga
      841 cccatctcat gggtctcttt gagcctggag acatgaaata cgagatccac cgagactcca
      901 cactggaccc ctccctgatg gagatgacag aggctgccct gcgcctgctg agcaggaacc
      961 cccgcggctt cttcctcttc gtggagggtg gtcgcatcga ccatggtcat catgaaagca
     1021 gggcttaccg ggcactgact gagacgatca tgttcgacga cgccattgag agggcgggcc
     1081 agctcaccag cgaggaggac acgctgagcc tcgtcactgc cgaccactcc cacgtcttct
     1141 ccttcggagg ctaccccctg cgagggagct ccatcttcgg gctggcccct ggcaaggccc
     1201 gggacaggaa ggcctacacg gtcctcctat acggaaacgg tccaggctat gtgctcaagg
     1261 acggcgcccg gccggatgtt accgagagcg agagcgggag ccccgagtat cggcagcagt
     1321 cagcagtgcc cctggacgaa gagacccacg caggcgagga cgtggcggtg ttcgcgcgcg
     1381 gcccgcaggc gcacctggtt cacggcgtgc aggagcagac cttcatagcg cacgtcatgg
     1441 ccttcgccgc ctgcctggag ccctacaccg cctgcgacct ggcgcccccc gccggcacca
     1501 ccgacgccgc gcacccgggt tactctagag tcggggcggc cggccgcttc gagcagacat
     1561 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     1621 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     1681 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     1741 tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc cgtcgaccga
     1801 tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg
     1861 tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc
     1921 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     1981 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     2041 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2101 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2161 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2221 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2281 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2341 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2401 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     2461 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     2521 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     2581 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     2641 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     2701 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     2761 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     2821 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     2881 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     2941 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     3001 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     3061 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     3121 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     3181 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     3241 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     3301 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     3361 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     3421 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     3481 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     3541 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     3601 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     3661 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     3721 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     3781 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     3841 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     3901 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     3961 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4021 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4081 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4141 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4201 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4261 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc ccattcgcca
     4321 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     4381 cccaagctac catgataagt aagtaatatt aaggtacggg aggtacttgg agcggccgca
     4441 ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac
     4501 taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc
     4561 ccagtgcaag tgcaggtgcc agaacatttc tctatcgata ggtaccgagc tcttacgcgt
     4621 gctagcgcac cagacagtga cgtcagctgc cagatcccat ggccgtcata ctgtgacgtc
     4681 tttcagacac cccattgacg tcaatgggag aacagatctg ccgccccgac tgcatctgcg
     4741 tgttcgaatt cgccaatgac aagacgctgg gcggggtttg tgtcatcata gaactaaaga
     4801 catgcaaata tatttcttcc ggggacaccg ccagcaaacg cgagcaacgg gccacgggga
     4861 tgaagca
//
