LOCUS       V008508                 5213 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008508
VERSION     V008508
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Kemmer C, Gitzinger M, Daoud-El Baba M, Djonov V, Stelling J,
            Fussenegger M.
  TITLE     Self-sufficient control of urate homeostasis in mice by a synthetic
            circuit
  JOURNAL   Nat. Biotechnol. 28 (4), 355-360 (2010)
   PUBMED   20351688
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Kemmer C, Gitzinger M, Daoud-El Baba M, Djonov V, Stelling J,
            Fussenegger M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2010) Department of Biosystems Science and
            Engineering, D-BSSE, ETH Zurich, Mattenstrasse 20, Basel 4058,
            Switzerland
REFERENCE   3  (bases 1 to 5213)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5213)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat.
            Biotechnol."; date: "2010"; volume: "28"; issue: "4"; pages:
            "355-360"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-NOV-2010) Department of Biosystems Science and Engineering,
            D-BSSE, ETH Zurich, Mattenstrasse 20, Basel 4058, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        52..431
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        432..634
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     repeat_region   643..904
                     /label="synthetic octameric hucO repeat (hucO8)"
                     /note="synthetic octameric hucO repeat (hucO8)"
     sig_peptide     922..984
                     /label="Ig-kappa leader"
                     /note="leader sequence from mouse immunoglobulin kappa
                     light chain"
     CDS             985..1887
                     /gene="uaZ"
                     /label="Uricase"
                     /note="Uricase from Aspergillus flavus. Accession#: Q00511"
     polyA_signal    complement(1930..2051)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     regulatory      2154..2399
                     /label="simian virus 40 enhancer"
                     /note="simian virus 40 enhancer"
                     /regulatory_class="enhancer"
     rep_origin      complement(2716..3304)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3478..4335)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4336..4440)
                     /label="AmpR promoter"
     rep_origin      4467..4922
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5053..5101
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    5115..5206
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgaggaa gatctcgcgt tgacattgat
       61 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
      121 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      181 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      241 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      301 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      361 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      421 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      481 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      541 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      601 ggcgtgtacg gtgggaggtc tatataagca agcttagacg tcgccctcag taggtagaca
      661 tctaagtatc tggcgccctc agtaggtaga catctaagta tctggcgccc tcagtaggta
      721 gacatctaag tatctggcgc cctcagtagg tagacatcta agtatctggc gatatcgccc
      781 tcagtaggta gacatctaag tatctggcgc cctcagtagg tagacatcta agtatctggc
      841 gccctcagta ggtagacatc taagtatctg gcgccctcag taggtagaca tctaagtatc
      901 tggccctgca ggaattccac catggagacc gatacactgc tgctgtgggt gctgctcctg
      961 tgggtgccag gaagtacagg agattctgct gtgaaggccg caagatatgg caaggataat
     1021 gtgagggtgt acaaggtgca taaggacgaa aagactggcg tgcagacagt gtacgagatg
     1081 accgtgtgcg tcctgctgga gggcgaaatc gagacttctt ataccaaagc cgacaactcc
     1141 gtgattgtgg ccacagattc tatcaagaac actatctata tcaccgccaa acagaaccca
     1201 gtgacaccac ctgaactgtt cggcagcatt ctcggcacac actttattga gaagtacaac
     1261 cacatccatg ctgcacacgt gaatatcgtg tgtcatcgct ggacccgcat ggacatcgac
     1321 ggaaagccac acccccactc ttttatcaga gactctgaag aaaagagaaa cgtgcaggtc
     1381 gacgtggtgg agggaaaagg tatcgacatc aagagctcac tctccggcct gaccgtgctg
     1441 aagagtacca attcacagtt ttgggggttt ctgagagacg aatacactac actgaaggag
     1501 acttgggata gaatcctgag taccgacgtg gatgcaacct ggcagtggaa gaatttttcc
     1561 gggctgcagg aagtgcggtc ccacgtgccc aagtttgatg caacctgggc aaccgcaagg
     1621 gaggtgacac tgaaaacctt tgccgaggac aactccgcta gcgtgcaggc cacaatgtac
     1681 aagatggccg aacagatcct ggccagacag cagctgattg agactgtgga gtactctctg
     1741 cctaacaagc actatttcga aatcgacctg tcctggcaca agggactgca gaatactggt
     1801 aaaaacgcag aggtgttcgc ccctcagagt gatcccaatg gtctgatcaa atgcacagtg
     1861 gggagatcct ctctgaagag caagctgtaa tctagagtcg gggcggccgg ccgcttcgag
     1921 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     1981 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     2041 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     2101 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatctga
     2161 acgatggagc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     2221 gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     2281 ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact
     2341 tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat tccacagcgg
     2401 atccgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg
     2461 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag
     2521 gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     2581 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     2641 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     2701 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     2761 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2821 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2881 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     2941 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3001 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3061 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3121 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     3181 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3241 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3301 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3361 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3421 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     3481 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     3541 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     3601 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     3661 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     3721 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     3781 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     3841 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     3901 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     3961 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4021 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4081 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     4141 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     4201 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     4261 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     4321 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     4381 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     4441 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg
     4501 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     4561 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     4621 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4681 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     4741 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     4801 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     4861 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     4921 ttcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     4981 gctattacgc cagcccaagc taccatgata agtaagtaat attaaggtac gggaggtact
     5041 tggagcggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt
     5101 gaatcgatag tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca
     5161 aaataggctg tccccagtgc aagtgcaggt gccagaacat ttctctatcg ata
//
