LOCUS       V015326                 5869 bp    DNA     circular SYN 12-DEC-2019
DEFINITION  Exported.
ACCESSION   V015326
VERSION     V015326
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Hartley CJ, Williams CC, Scoble JA, Churches QI, North A, French NG,
            Nebl T, Coia G, Warden AC, Simpson G, Frazer AR, Nixon Jensen C,
            Turner NJ, Scott C.
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   Hartley CJ, Williams CC, Scoble JA, Churches QI, North A, French NG,
            Nebl T, Coia G, Warden AC, Simpson G, Frazer AR, Nixon Jensen C,
            Turner NJ, Scott C.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5869)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (10-MAY-2019) Biocatalysis "
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-MAY-2019) Biocatalysis "
FEATURES             Location/Qualifiers
     source          1..5869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        70..88
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             120..142
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             161..178
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             188..205
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     CDS             209..1225
                     /gene="gpsA"
                     /label="Glycerol-3-phosphate dehydrogenase [NAD(P)+]"
                     /note="Glycerol-3-phosphate dehydrogenase [NAD(P)+] from
                     Escherichia coli (strain SMS-3-5 / SECEC). Accession#:
                     B1LK47"
     terminator      3581..3628
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     promoter        complement(3994..4022)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     promoter        4135..4239
                     /label="AmpR promoter"
     CDS             4240..5097
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5271..5859
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg gatctcgatc
       61 ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct agaaataatt
      121 ttgtttaact ttaagaagga gatataccat gggcagcagc catcatcatc atcatcacag
      181 cagcggcctg gtgccgcgcg gcagccatat gaatcagcgt aatgcaagca tgaccgttat
      241 tggtgcaggt agctatggca ccgcactggc aattaccctg gcacgtaatg gtcatgaagt
      301 tgttctgtgg ggtcatgatc cggaacacat tgcaaccctg gaacgtgatc gttgtaatgc
      361 agcatttctg ccggatgttc cgtttccgga taccctgcat ctggaaagcg atctggcaac
      421 agcactggca gcaagccgta atattctggt tgttgttccg agccatgttt ttggtgaagt
      481 tctgcgtcag attaaaccgc tgatgcgtcc ggatgcacgt ctggtttggg caaccaaagg
      541 tctggaagca gaaaccggtc gtctgctgca ggatgttgca cgtgaagcac tgggtgatca
      601 gattccgctg gcagttatta gcggtccgac ctttgcaaaa gaactggcag ccggtctgcc
      661 gaccgccatt agcctggcaa gcaccgatca gacatttgcc gatgatctgc agcaactgct
      721 gcattgtggt aaaagctttc gtgtttatag caacccggat tttattggtg ttcagctggg
      781 tggtgcagtg aaaaatgtta ttgcaattgg tgccggtatg agtgatggta ttggttttgg
      841 tgcaaatgca cgtaccgcac tgattacccg tggtctggcc gaaatgagcc gtctgggtgc
      901 agccctgggt gccgatccgg caacctttat gggtatggca ggtctgggcg atctggttct
      961 gacctgtacc gataatcaga gccgtaatcg tcgttttggt atgatgctgg gtcagggtat
     1021 ggatgttcag agcgcacaag aaaaaattgg tcaggttgtt gaaggttacc gcaataccaa
     1081 agaagtgcgc gaactggcac atcgttttgg cgttgaaatg ccgattaccg aagaaatcta
     1141 tcaggttctg tattgcggca aaaatgcccg tgaagcagca ctgaccctgc tgggtcgtgc
     1201 acgtaaagat gaacgtagca gtcatggtac cagcggtagc agcggttcaa gctgtggtag
     1261 cagtggtagc tcaggtagca gcatgaaaat tgttgttatt ggttgtaccc atgcaggcac
     1321 cgcagcagtt aaaaccattc tgaaagaaaa tccggaagcc gaaatcacca tttttgaacg
     1381 caatgacaac atcagctttc tgagctgcgg tattgcactg tatgttggtg gtgttgttaa
     1441 agatccggca ggcctgtttt attcaaaccc ggaagaactg agcaaaatgg gtgccaatgt
     1501 taaaatcaaa cataacgtga aaagcatcga caccaaaagc aaaaaagtga tcgccgaaga
     1561 tatgaacacc ggtgaagaaa ttgaagtgag ctatgataaa ctggtgaata ccaccggtag
     1621 ctggccgatt attccgccta ttccgggtat cgaaagtaaa aacattctgc tgtgcaaaaa
     1681 ctatgatcag gccaatgtga ttattcgcca gaccaaagat gccaaaaaaa tcgtgattgt
     1741 gggtggtggt tatattggta ttgaactggt tgaagccttt cagaaaagcg gtaaacaggt
     1801 taccctgatt gatggtctgg atcgtattct gaacaaatac ctggataaag agttcaccga
     1861 tatcctggaa gatgatctga aaaaaaacgg cattaatctg gcactggatc agtgtgtgaa
     1921 aagcttcaaa gcaaatgaaa atggcgaagt taccagcgtt gaaaccacca aaggtgaata
     1981 tgaagccgat atggttattc tgtgtgttgg ttttcgtccg aataacgaac tgctgaaagg
     2041 taaagttgat atgctgccga atgatgccat tatcgtggat gaatatatgc gtaccagcga
     2101 tccggatatt tttgcagccg gtgatagctg tgcagttcat tataatccga atggcaacta
     2161 tgcctatatc ccgctggcaa ccaatgcagt tcgcatgggt atgctgattg gcaaaaacat
     2221 tagcaccccg aaagttaaat atcgtggcac ccagagcacc agtggtctga acctgtttgg
     2281 ctataacatt ggtagcaccg gtgtgaccgt ttcaggtgca ccgcagattg gtctgaatgt
     2341 tcgtagcgtt attgtgaaag ataactaccg tccggaattt atgccgacca atgaagaaat
     2401 cattatgcag ctggtttatg aagttggcac caatcgtatt gttggcggtc aggttatgag
     2461 caaatatgat attacccaga gcgcaaatac cctgagcctg gccattcaga ataaaatgac
     2521 cattgaagat ctggcctatg tggatttctt ttttcagccg cattttgatc gtccgtggaa
     2581 ctatctgaac attctgggtc tggcagcact ggaacaagag ggtctgatgc cgctggaccc
     2641 ggttattcag caggttctgg atcagctgaa tcgtatgcct gcacctgatt ataaacatct
     2701 gagcgcacag cagtttcgta gccagcagag cctgtttccg cctgttaaaa aagaaccggt
     2761 tgccgaagtt cgtgaatttg atatggatct gcctggtcgt accctgaaag ttcgtatgta
     2821 tcgtccggaa ggtgttgaac cgccttatcc ggcactggtt tattatcatg gtggtggctg
     2881 ggttgttggt gatctggaaa cccatgatcc tgtttgtcgt gttctggcaa aagatggtcg
     2941 tgcagttgtt tttagcgttg attatcgtct ggcacctgaa cataaatttc cggcagccgt
     3001 tgaagatgcc tatgatgccc tgcagtggat tgcagaacgt gcagcagatt ttcatctgga
     3061 ccctgcacgt attgcagtgg gtggcgatag tgccggtggc aatctggcag cggttaccag
     3121 cattctggcc aaagaacgtg gtggtcctgc actggcattt cagctgctga tttatccgag
     3181 caccggttat gatccggcac atccgcctgc aagcattgaa gaaaatgcag aaggttatct
     3241 gctgaccggt ggcatgatgc tgtggtttcg tgatcagtat ctgaatagcc tggaagaact
     3301 gacccatccg tggtttagtc cggtgctgta tcctgatctg agcggtctgc ctccggcata
     3361 tattgcaacc gcacagtatg atccgctgcg tgatgttggt aaactgtatg cagaagcact
     3421 gaataaagcc ggtgttaaag tggaaatcga aaactttgag gatctgattc atggctttgc
     3481 ccagttttat agcctgagtc cgggtgccac caaagcactg gttcgtattg ccgaaaaact
     3541 gcgtgacgca ctggcctaat aaggatccgc tgagcaataa ctagcataac cccttggggc
     3601 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca
     3661 ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca
     3721 ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt
     3781 gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga
     3841 cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca
     3901 gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac
     3961 tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc
     4021 aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     4081 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     4141 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4201 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4261 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4321 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4381 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4441 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     4501 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4561 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4621 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4681 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4741 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     4801 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4861 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4921 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4981 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5041 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5101 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     5161 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5221 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5281 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5341 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5401 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     5461 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5521 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5581 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5641 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5701 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5761 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5821 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagca
//
