LOCUS       62056_6475        3396 bp DNA     circular SYN 12-DEC-2019
DEFINITION  Cloning vector pCJH6_pETcc2::CaNOX, complete sequence.
ACCESSION   MK910754
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Hartley CJ, Williams CC, Scoble JA, Churches QI, North A, French NG,
            Nebl T, Coia G, Warden AC, Simpson G, Frazer AR, Nixon Jensen C, 
            Turner NJ, Scott C.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Hartley CJ, Williams CC, Scoble JA, Churches QI, North A, French NG,
            Nebl T, Coia G, Warden AC, Simpson G, Frazer AR, Nixon Jensen C, 
            Turner NJ, Scott C.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 3396)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (10-MAY-2019) Biocatalysis "
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-MAY-2019) Biocatalysis "
FEATURES             Location/Qualifiers
     source          1..3396
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4..1350
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /EC_number="1.6.3.4"
                     /label=NADH oxidase
                     /note="from Clostridium aminoverlaricum (BAE53714)"
                     /protein_id="QGT40742.1"
                     /translation="MKIVVIGCTHAGTAAVKTILKENPEAEITIFERNDNISFLSCGIA
                     LYVGGVVKDPAGLFYSNPEELSKMGANVKIKHNVKSIDTKSKKVIAEDMNTGEEIEVSY
                     DKLVNTTGSWPIIPPIPGIESKNILLCKNYDQANVIIRQTKDAKKIVIVGGGYIGIELV
                     EAFQKSGKQVTLIDGLDRILNKYLDKEFTDILEDDLKKNGINLALDQCVKSFKANENGE
                     VTSVETTKGEYEADMVILCVGFRPNNELLKGKVDMLPNDAIIVDEYMRTSDPDIFAAGD
                     SCAVHYNPNGNYAYIPLATNAVRMGMLIGKNISTPKVKYRGTQSTSGLNLFGYNIGSTG
                     VTVSGAPQIGLNVRSVIVKDNYRPEFMPTNEEIIMQLVYEVGTNRIVGGQVMSKYDITQ
                     SANTLSLAIQNKMTIEDLAYVDFFFQPHFDRPWNYLNILGLAALEQEGL"
     promoter        1457..1561
                     /label=AmpR promoter
     CDS             1562..2419
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2593..3181
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3261..3279
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             3311..3333
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3352..3369
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             3379..3396
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
ORIGIN
        1 catatgaaaa tcgtggtgat tggttgtacc catgcaggca ccgcagcagt taaaaccatt
       61 ctgaaagaaa atccggaagc cgaaatcacc atttttgaac gcaatgacaa catcagcttt
      121 ctgagctgtg gtattgcact gtatgttggt ggtgttgtta aagatccggc aggtctgttt
      181 tatagcaacc cggaagaact gagcaaaatg ggtgcaaatg tgaaaatcaa acataacgtg
      241 aaaagcatcg ataccaaaag caaaaaagtg atcgccgaag atatgaacac cggtgaagaa
      301 attgaagtga gctatgataa actggtgaat accaccggta gctggccgat tattccgcct
      361 attccgggta ttgaaagtaa aaacattctg ctgtgcaaaa actatgatca ggccaatgtt
      421 attatccgcc agaccaaaga tgccaaaaaa atcgttattg tgggtggtgg ctatattggt
      481 attgaactgg ttgaagcatt tcagaaaagc ggtaaacagg ttaccctgat tgatggtctg
      541 gatcgtattc tgaacaaata cctggataaa gagttcaccg atatcctgga agatgatctg
      601 aaaaaaaacg gcattaatct ggcactggat cagtgcgtga aatcctttaa agcaaatgaa
      661 aatggcgaag tgaccagcgt tgaaaccacc aaaggtgaat atgaagccga tatggttatt
      721 ctgtgtgttg gttttcgtcc gaataacgaa ctgctgaaag gtaaagttga tatgctgccg
      781 aatgatgcca ttatcgtgga tgaatatatg cgtaccagcg atccggatat ttttgcagcc
      841 ggtgatagct gtgcagttca ttataatccg aatggcaact atgcctatat tccgctggca
      901 accaatgcag ttcgtatggg tatgctgatt ggcaaaaaca ttagcacccc gaaagttaaa
      961 tatcgtggca cccagagcac cagcggtctg aacctgtttg gctacaatat tggtagtacc
     1021 ggtgttaccg ttagtggtgc accgcagatt ggtctgaatg ttcgtagcgt gattgtgaaa
     1081 gataactacc gtccggaatt tatgccgacc aatgaagaaa tcattatgca gctggtttat
     1141 gaagttggca ccaatcgtat tgttggcggt caggttatga gcaaatatga tattacccag
     1201 agcgcaaata ccctgagcct ggcaattcag aataaaatga ccattgagga tctggcctat
     1261 gtggatttct tttttcagcc gcattttgat cgtccgtgga actatctgaa tattctgggt
     1321 ctggcagcac tggaacaaga aggtctgtaa gaattcttga agacgaaagg gcctcgtgat
     1381 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     1441 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     1501 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     1561 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     1621 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     1681 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     1741 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     1801 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     1861 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     1921 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     1981 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     2041 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     2101 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     2161 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     2221 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     2281 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     2341 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     2401 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     2461 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     2521 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     2581 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2641 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2701 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     2761 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2821 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2881 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2941 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3001 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3061 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3121 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3181 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3241 ggatctcgat cccgcgaaat taatacgact cactataggg agaccacaac ggtttccctc
     3301 tagaaataat tttgtttaac tttaagaagg agatatacca tgggcagcag ccatcatcat
     3361 catcatcaca gcagcggcct ggtgccgcgc ggcagc
//
