LOCUS       V008511                 7548 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008511
VERSION     V008511
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7548)
  AUTHORS   Weaver KE, Chen Y, Miiller EM, Johnson JN, Dangler AA, Manias DA,
            Clem AM, Schjodt DJ, Dunny GM.
  TITLE     Examination of E. faecalis toxin-antitoxin (TA) toxin Fst function
            utilizing a pheromone-inducible expression vector with tight
            repression and large dynamic range
  JOURNAL   J. Bacteriol. (2017) In press
   PUBMED   28348028
REFERENCE   2  (bases 1 to 7548)
  AUTHORS   Weaver K, Chen Y, Miiller E, Johnson J, Dangler A, Manias D, Clem A,
            Schjodt D, Dunny G.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7548)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7548)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol. (2017) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-MAR-2017) Department of Microbiology "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7548
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(74..1027)
                     /codon_start=1
                     /gene="prgX"
                     /product="PrgX"
                     /label="prgX"
                     /protein_id="ARF06186.1"
                     /translation="MFKIGSVLKQIRQELNYHQIDLYSGIMSKSVYIKVEADSRPISVE
                     ELSKFSERLGVNFFEILNRAGMNTKSVNETGKEKLLISKIFTNPDLFDKNFQRIEPKRL
                     TSLQYFSIYLGYISIAHHYNIEVPTFNKTITSDLKHLYDKRTTFFGIDYEIVSNLLNVL
                     PYEEVSSIIKPMYPIVDSFGKDYDLTIQTVLKNALTISIMNRNLKEAQYYINQFEHLKT
                     IKNISINGYYDLEINYLKQIYQFLTDKNIDSYLNAVNIINIFKIIGKEDIHRSLVEELT
                     KISAKEKFTPPKEVTMYYENYVAIENNPIPEIKEQS"
     gene            complement(74..1027)
                     /gene="prgX"
                     /label="prgX"
     regulatory      1206..1214
                     /regulatory_class="promoter"
     CDS             1236..1307
                     /codon_start=1
                     /gene="prgQ"
                     /product="PrgQ"
                     /label="prgQ"
                     /protein_id="ARF06187.1"
                     /translation="MKTTLKKLSRYIAVVIAITLIFI"
     gene            1236..1307
                     /gene="prgQ"
                     /label="prgQ"
     regulatory      1556..1600
                     /regulatory_class="terminator"
     regulatory      1699..1749
                     /regulatory_class="terminator"
     CDS             complement(2152..2163)
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             2625..3272
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(3619..4779)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B"
                     /label="repB"
                     /protein_id="ARF06189.1"
                     /translation="MSSISKNEPNQKQVQTLNELSKRKVVEHNSLITSIAKMDKTPLKM
                     FELAVSCINTEEPPKDHTVYLSKEELFAFFKVSDNDKHSRFKQAVENMQKQAFFQIKEE
                     VGKGFKFRSIVPIPYVEWTDYHDDVKIEFHREIMPYLINLKQNFTQHALSDIAELNSKY
                     SIILYRWLSMNYNQYEHYSYKGGRREEQVEAYRNPTISMRELREMTDTVDEYPRFDRLE
                     HRVLKEPIEEINENTSFNVTYDKIKKGRSIDSIVFHITKKRRADDNSYKLEDKDYQSDK
                     EEKSRNEADLLKQAMESKYTRLLIENFLLSPLEMTDTALMAGLQKNVYPLYDELKELRG
                     LNGVKDHLSYISSKKEAYSKRNVAKYLKKAIEQYLPTVKRQDLNHE"
     gene            complement(3619..4779)
                     /gene="repB"
                     /label="repB"
     misc_feature    6144..6284
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6470..7058)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 attttgttgg tggcttggaa atgtttaggc accttatata ataaaaaata aaagagcagt
       61 catgaaaata ttttcatgac tgctctttta tttcaggaat aggattattt tctattgcaa
      121 cgtaattttc ataatacatg gttacttctt tagggggcgt aaatttttct tttgcagata
      181 tttttgtaag ttcctcaact aaagatctgt gaatatcttc ttttccaatg attttaaaga
      241 tgttgataat atttaccgca tttaaataag aatcaatatt tttgtcagtt aaaaattgat
      301 atatttgttt taaatagttt atttctaagt cgtaatatcc atttatgcta atatttttaa
      361 ttgtttttaa gtgttcaaat tgattaatat aatactgcgc ttcttttaga tttctattca
      421 taattgatat tgttaaagca ttttttagaa cagtctgaat agttaaatcg taatctttac
      481 caaaggaatc cacgattgga tacataggct taatgatact tgaaacttct tcatacggta
      541 agacatttaa aagatttgaa acaatttcat aatcaattcc aaaaaatgtt gttcttttat
      601 catataaatg ttttaaatct gaagtaattg ttttattaaa agtaggaact tcaatgttat
      661 aatgatgtgc aatagaaata tatccaagat aaatactaaa gtactgaaga ctagttaagc
      721 gctttggctc aatcctttga aaatttttgt caaataagtc agggtttgta aatatttttg
      781 aaataagaag tttttctttt cctgtttcat ttacgctttt tgtattcatg ccagctctat
      841 ttaaaatttc aaaaaaattt acacctaaac gttctgagaa cttgcttaac tcttctacag
      901 aaatcggtcg agagtcagct tcaactttta tgtaaacaga tttggacatt attccagaat
      961 ataaatcaat ctgatggtaa tttaactctt gccttatttg tttcaggaca gaacctatct
     1021 taaacatttt cttcctccta atatctcgag tatcaaaaag tagacctaaa attcgataaa
     1081 ctacaaaaat ttgttaatat tttaatttta ggtattgaat acgacactcg aagatgtgtt
     1141 tattaagcta tatccctttt ttttaaaaaa aaatacatat tttagttgaa aatataatac
     1201 ttagatgtta agatgttttt ataggagggg tgtaaatgaa aaccactcta aaaaaactat
     1261 caagatatat agctgttgta attgcaataa ccttaatatt tatctgatag aaaaaatcat
     1321 agtaacaatt aaacaaatta agaaccgact gccataggac ggcaatccta gggggcagtt
     1381 aaacaattca tggtatgaca tgaactctac tcggttctcg tttgttgcaa cattagttac
     1441 aacgtatagt ataacaattt tttatgtaaa attctagact tttttaaact cctttatttg
     1501 tctaggaaaa gtttttacag tgaattgttt ttattagttg tataaatgtt ggagcagcgg
     1561 ggaatgtata cagttcatgt atatgttccc cgcttttttt gttgtctgtt gggggatccg
     1621 tcgacgcatg caagcttcct aaagaagtaa ccatgtatta tgaaaattac gttgcaatag
     1681 aaaataatcc tattcctgaa ataaaagagc agtcatgaaa atattttcat gactgctctt
     1741 ttatttttta ttatataagg tgcctaaaca tttccaagcc accaacaaaa tgaattcctt
     1801 aaggaacgta cagacggctt aaaagccttt aaaaacgttt ttaaggggtt tgtagacaag
     1861 gtaaaggata aaacagcaca attccaagaa aaacacgatt tagaacctaa aaagaacgaa
     1921 tttgaactaa ctcataaccg agaggtaaaa aaagaacgaa gtcgattttt tattaaaacg
     1981 tctcaaaatc gtttctgaga cgttttagcg tttatttcgt ttagttatcg gcataatcgt
     2041 taaaacaggc gttatcgtag cgtaaaagcc cttgagcgta gcgtggcttt gcagcgaaga
     2101 tgttgtctgt tagattatga aagccgatga ctgaatgaaa taataagcgc agcgtccttc
     2161 tatttcggtt ggaggaggct caagggagtt tgagggaatg aaattccctc atgggtttga
     2221 ttttaaaaat tgcttgcaat tttgccgagc ggtagcgctg gaaaattttt gaaaaaaatt
     2281 tggaatttgg aaaaaaatgg ggggaaagga agcgaatttt gcttccgtac tacgaccccc
     2341 cattaagtgc cgagtgccaa tttttgtgcc aaaaacgctc tatcccaact ggctcaaggg
     2401 tttgaggggt ttttcaatcg ccaacgaatc gccaacgttt tcgccaacgt tttttataaa
     2461 tctatattta agtagcttta ttgttgtttt atgattacaa agtgatacac taattttata
     2521 aaattatttg attggagttt tttaaatggt gatttcagaa tcgaaaaaaa gagttatgat
     2581 ttctctgaca aaagagcaag ataaaaattt aggaggcata tcaaatgaac tttaataaaa
     2641 ttgatttaga caattggaag agaaaagaga tatttaatca ttatttgaac caacaaacga
     2701 cttttagtat aaccacagaa attgatatta gtgttttata ccgaaacata aaacaagaag
     2761 gatataaatt ttaccctgca tttattttct tagtgacaag ggtgataaac tcaaatacag
     2821 cttttagaac tggttacaat agcgacggag agttaggtta ttgggataag ttagagccac
     2881 tttatacaat ttttgatggt gtatctaaaa cattctctgg tatttggact cctgtaaaga
     2941 atgacttcaa agagttttat gatttatacc tttctgatgt agagaaatat aatggttcgg
     3001 ggaaattgtt tcccaaaaca cctatacctg aaaatgcttt ttctctttct attattccat
     3061 ggacttcatt tactgggttt aacttaaata tcaataataa tagtaattac cttctaccca
     3121 ttattacagc aggaaaattc attaataaag gtaattcaat atatttaccg ctatctttac
     3181 aggtacatca ttctgtttgt gatggttatc atgcaggatt gtttatgaac tctattcagg
     3241 aattgtcaga taggcctaat gactggcttt tataatatga gataatgccg actgtacttt
     3301 ttacagtcgg ttttctaacg atacattaat aggtacgaaa aagcaacttt ttttgcgctt
     3361 aaaaccagtc ataccaataa cttaagggta actagcctcg ccggaaagag cgaaaatgcc
     3421 tcacatttgt gccacctaaa aaggagcgat ttacatatga gttatgcagt ttgtagaatg
     3481 caaaaagtga aatcagctag atatatagtg ccgtctaatt tttgtatcaa atcactatct
     3541 aatttagata cttgataatg aattttcttt tttgatacgc ccaactcatc agccaactct
     3601 ttaatcgttt ttaagttctc actcatggtt taagtcctgc cttttaaccg taggtagata
     3661 ttgctcgatt gcttttttca gatacttcgc tacattgcgt ttagaatagg cttctttttt
     3721 gctagatata taagacaagt ggtctttgac cccattcaat cctcttaatt cctttaactc
     3781 gtcatacaac ggatagacgt tcttttgcaa acctgccata agtgccgtgt ccgtcatttc
     3841 aagaggggat aagagaaagt tttcaatcaa taatcgtgtg tacttgcttt ccattgcctg
     3901 ttttaataag tcagcttcat ttcttgattt ttcctctttg tcggattgat aatctttatc
     3961 ttctaacttg tagctgttat catctgctcg acgttttttc gtgatatgaa agacaataga
     4021 atcaatgctt cgtccttttt ttatcttgtc atacgtcacg ttaaaagagg tgttttcgtt
     4081 aatttcttct attggttctt ttaaaactct atgttctaat ctatcaaagc gggggtattc
     4141 atcaactgta tccgtcattt ctcgtaattc tcgcattgaa atggtaggat tgcggtaggc
     4201 ttccacttgt tcttctctcc gtccgccctt ataactataa tgctcgtatt ggttataatt
     4261 catggataac caacggtaca agataataga gtatttgcta ttcagctctg caatatcaga
     4321 caaagcatgt tgcgtgaaat tttgttttag attaattaag tagggcatga tttcacgatg
     4381 aaattcaatt tttacgtcat catgataatc tgtccactcg acatatggaa tgggaacaat
     4441 actcctaaat ttaaatcctt tacctacttc ttctttaatt tgaaaaaagg cttgtttttg
     4501 catattctct actgcttgtt taaaacgact atgtttgtca ttatcagata ccttaaaaaa
     4561 ggcaaacaat tcttcttttg agagataaac cgtatgatct ttgggtggtt cttcggtatt
     4621 aatacaagac acggctaatt caaacatttt cagtggcgtt ttatccattt tcgcaatact
     4681 ggtaattaaa gaattatgtt ccactacttt tcgttttgac aattcgttca aggtttgcac
     4741 ctgcttttga ttaggttcat tttttgatat actagacata gaaaactgct cctttgtgag
     4801 tgagtttaga taagcatgaa gaaacacttt tcgtagagag attcttcatg cttttattat
     4861 atcattatat accaacgaaa caaacaaaga agttggtata tgcacagttt tttgttggtg
     4921 tatgcacagt tttttgttgg tatatacaca gttttttgtt ggtatataat tctataaagc
     4981 cttgaaaata ttgatttttt tttgacgcaa aagaataaaa gattaaaata tataagataa
     5041 atatataata ggcttcgcct attttttatt tttttcaaaa atttaaaacc aaaggtcaaa
     5101 gtcatcaaaa aaaggagtga gcgagtgaac gctcacattt tacttgcagc gttcactcgc
     5161 taaaagaaaa gatcaaaacc gcttttagga atttattgga ttccctctaa ttgctcatat
     5221 aagctgtttt aactgtttga ctagctaaat actcatatca tgcaatcaag cgtcttacag
     5281 acggtcaggg caagccctaa caacccttaa atggaaaact gatactttgt ttaacttgtt
     5341 ttttttacaa cttacaacaa gctattcaat tcaaaatcaa tttaaacccc cttaaaagct
     5401 cgtttaagca ttcttaatgc tccctttgaa caaatagaca gtttcgaatt aaagtcgctt
     5461 aaatcgagtt ttagaacgtt ttaaaataga atgatgaaaa actaaaaagt ttcaaacgct
     5521 ggtcggcttg tttcgtaaaa aaagagtgca atgcgcactt acacatcact ctaaaaacga
     5581 gtgatgtaat ggctaaaaat cgtgtattag aagttgctta tcacaacaac aaaatttcat
     5641 gatagtagct tctcttaaat gagtgagtct ttgtttcaat ttgtgataca tttttctgat
     5701 ccatcaataa cttactcaaa gataaaatgc ctcagcgctc attcctcaat cgccctttgt
     5761 cattgatttt gtaataagca ttgcgcccta ttcggttaca atcctcacaa caaaatagac
     5821 catataaaac cataaatact gcaagctatc acattgtaat acaaaacact tcgttcttgt
     5881 attacaatgt ggcttgaaaa gggggaagtt tcactttccc cttttatccc ctttggattg
     5941 tgtcgatttg ctttacacaa acgccacctg cccattttat aaaaataaaa tgggcaaaag
     6001 atctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     6061 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     6121 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     6181 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     6241 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     6301 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6361 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6421 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6481 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6541 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6601 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6661 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6721 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6781 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6841 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6901 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6961 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7021 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7081 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7141 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7201 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7261 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7321 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7381 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7441 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7501 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcag
//
