LOCUS       40924_10731        8922 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCIB4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8922)
  AUTHORS   Dequard-Chablat M, Nguyen T-T., Contamine V, Hermann-Le Denmat S, 
            Malagnac F.
  TITLE     Efficient tools to target DNA to Podospora anserina
  JOURNAL   Fungal Genet Rep 59, 21-25 (2012)
REFERENCE   2  (bases 1 to 8922)
  AUTHORS   Dequard-Chablat M, Nguyen T-T., Contamine V, Hermann-Le Denmat S, 
            Malagnac F.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2012) IGM, UPS XI, CNRS-UMR 8621, 15 rue Georges 
            Clemenceau, Orsay, Ile de France 91405, France
REFERENCE   3  (bases 1 to 8922)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8922)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Rep 59, 21-25 (2012)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-JUN-2012) IGM, UPS XI, CNRS-UMR 8621, 15 rue Georges Clemenceau,
            Orsay, Ile de France 91405, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        196..416
                     /label=URA3 promoter
     CDS             417..1217
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     1951..1967
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1977..1995
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1995..2002
                     /label=modified AscI restriction site
                     /note="modified AscI restriction site"
     5'UTR           2003..2963
                     /gene="Pa_4_5450"
     gene            2003..2963
                     /gene="Pa_4_5450"
                     /label=Pa_4_5450
                     /note="from Podospora anserina chromosome 4"
     misc_feature    2963..2970
                     /label=modified SwaI restriction site
                     /note="modified SwaI restriction site"
     regulatory      2971..3306
                     /gene="GPD"
                     /gene_synonym="Pa_3_5110"
                     /regulatory_class="promoter"
     gene            2971..3306
                     /gene="GPD"
                     /gene_synonym="Pa_3_5110"
                     /label=GPD
     CDS             3313..3876
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     regulatory      3929..4318
                     /gene="AS1"
                     /gene_synonym="Pa_1_16650"
                     /regulatory_class="terminator"
     gene            3929..4318
                     /gene="AS1"
                     /gene_synonym="Pa_1_16650"
                     /label=AS1
     misc_feature    4319..4326
                     /label=modified PacI restriction site
                     /note="modified PacI restriction site"
     3'UTR           4327..5285
                     /gene="Pa_4_5450"
     gene            4327..5285
                     /gene="Pa_4_5450"
                     /label=Pa_4_5450
                     /note="from Podospora anserina chromosome 4"
     misc_feature    5285..5292
                     /label=modified AscI restriction site
                     /note="modified AscI restriction site"
     promoter        complement(5320..5338)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5359..5375)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5383..5399)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5407..5437)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5452..5473)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5761..6349)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6523..7380)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7381..7485)
                     /label=AmpR promoter
     rep_origin      complement(7512..8854)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     1981 acgactcact atagggcgcg ccgtgtgcgc taaacgttgg gtaatgtttc accgaaaagc
     2041 agaagaaaac gtcgttgatc actgacggcc cggtccgaga ctttaccaca acatctcttt
     2101 tttcgtccga cttgctctca gctcagctga ctttgatcac tgttgctgtt gcgggagtgc
     2161 gtgcgtcatc ggatgtcccg cgtcaccccg accacaattt ccgtcacgca aagggtttcg
     2221 gagaagagcg gtatggcctc gcggggctgg caaggagaag aaaagagttt gcgtgtctgt
     2281 tgagacagag agaggcaagg gactacggga ctgtgcggta gcgatcaaga taaagttttt
     2341 tttccttgtg ttgctgggca tgggcgggag gcagagagac gggaggaatg gagggggtgg
     2401 gaagtggtta gtttaaccct tacccagggc gctagcaggc atgaagggaa ccctgaggaa
     2461 aaatcatcaa ctgcggccag ttcaacattg ccattcagag aagggctggc tcaggctgag
     2521 tttggtgtaa gatgagttgt cactggaata gaagggcaat tattgtcttc tcaaaagttc
     2581 tcaggtgtgc aaaagcgaca tgtggcggcc aggtttcgca gccatttcct cttttgggga
     2641 gtggagggct ggctgcctct cagctggggc ctgagagagt gttacagcag ggtagagctg
     2701 agccatgtta atggctcggc gagcgtgatc ggtgcccaaa accaaccgcc gaacgacgtg
     2761 tcccagcgct ttggtagctg tgcgccaagt ctaattattg gcgggtagtg tgtggctttg
     2821 gacatgtcga acaatggcgg aatttaatac tccggcaaga aggaagggat gcacgatgct
     2881 gatcgagggg tatatacctg agagattgcc ctcctcttcc agggcagtgt ctgttcggaa
     2941 aggacaggcc agataaggta ccatttaaat cttcccttcc acttcttcac acagaccaca
     3001 cacacatcag gtaagtccca tcctcgagta agatcaactg gaaccccgca cctcctgcct
     3061 caccccgcct gcatcggacc ttctcgagcg acaccagctc ccacccaatg gctgcccggt
     3121 cccggactag gtggccagcc tgaggactgg aaaaacttta ttatctcgcg tctctccctc
     3181 aaaagctctc tctccatctc ctcgtctctt cttctcttcg acatctcatc ttcctgcaat
     3241 tccctggaaa cgaaacagct tactcaccat ctctctctct ctctctacta acagcaaagg
     3301 atccatatgg gtaccactct tgacgacacg gcttaccggt accgcaccag tgtcccgggg
     3361 gacgccgagg ccatcgaggc actggatggg tccttcacca ccgacaccgt cttccgcgtc
     3421 accgccaccg gggacggctt caccctgcgg gaggtgccgg tggacccgcc cctgaccaag
     3481 gtgttccccg acgacgaatc ggacgacgaa tcggacgacg gggaggacgg cgacccggac
     3541 tcccggacgt tcgtcgcgta cggggacgac ggcgacctgg cgggcttcgt ggtcgtctcg
     3601 tactccggct ggaaccgccg gctgaccgtc gaggacatcg aggtcgcccc ggagcaccgg
     3661 gggcacgggg tcgggcgcgc gttgatgggg ctcgcgacgg agttcgcccg cgagcggggc
     3721 gccgggcacc tctggctgga ggtcaccaac gtcaacgcac cggcgatcca cgcgtaccgg
     3781 cggatggggt tcaccctctg cggcctggac accgccctgt acgacggcac cgcctcggac
     3841 ggcgagcagg cgctctacat gagcatgccc tgcccctaac tgcagacgcg ttacgtatcg
     3901 gatccagaat tcgtgattag ggcccggggg gctggatatt gtggttcttg cattcagtct
     3961 ctcatgggtt cggcgaaacg ggacaacggt ctgctcacgg gcatgatgcg ctatgcgtgc
     4021 ggtgcctggg ctatcttggt tttagggaaa agatagtaca caaaagagca attcgagtgc
     4081 tggttccgac aacaacaaac gcacaacgct tgatggtgcg ctctgaagtg atgaacagag
     4141 agctcctgga gggacctctt catgatgatt catgccaaca gaaaagggac agacgtctag
     4201 gccgtagtta tcagtctgaa attagaccaa taacacattc ctgattcgag caattcttga
     4261 tgccagcatt tgcgtgtatt gtgtcccggt tctgtttgcc ttcttgatgc tttgtgcctt
     4321 aattaaggtc acaccgaact gagaaggggt tggctttgca tgctgatctt acggttgtgg
     4381 tagacaaact tggctaatac ttttcattgg ggctgtcata tcaggcttga tctccacagg
     4441 catattcggg cgttttgggg gacatttgaa cagcagggtg gaacatcatg atacctactt
     4501 tattcttctt gtcagaattc agattcgttt gttgctgtat ccggttcaca ttcaactggt
     4561 gaaatctcgc gtgcttggaa gcatgctgct gacttgcagc atgcgggcag ttgtgggctg
     4621 gtgagtaggt ttccagcaaa caacaacact gtacctaggt attttaccct gaggtagtaa
     4681 gccaatatat ggtatgcgag agtgtttgaa ggagatgggt ggtcaagtgc ttaggtggga
     4741 cagtataaag atcttgcgaa agtcgaaaga gtttccagaa agaaacctag cttgtgtgaa
     4801 ctttggtggt ataggtaaaa gccagacagt ccgatccgtc ccagaggtca tggaacatga
     4861 gccctgcatc aagagcggct cggcgccgga gctcaacttc cgatcatccc cgccacgttc
     4921 tcgcggccgg cagcaccaat gctacgatgc aggggacaat gcggccgtcg atttgttgca
     4981 gtgtcatccc aatcgtcatg aggtaagcaa tctggtatgc tgaccgctac ttgtgacaga
     5041 gaccggagag agagaagcca gagtaaacgt tcgattgttt ggtacatcaa atcggagctc
     5101 atggcgttca gcccggtttc atggtgttgt tgggccaagt gctctcattc tgacgtattg
     5161 tagcgttttt ccaagatgaa aagctgttgg ttttgtgata catcgttata ggaaactcgt
     5221 gatttgtcta tcaagtatga gtacctaccg ggtagaatgc tgatagctag gtctgcgaag
     5281 gtgtggcgcg ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc
     5341 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     5401 tccacacaac ataggagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     5461 gtaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     5521 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     5581 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     5641 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     5701 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     5761 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     5821 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     5881 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     5941 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6001 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6061 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6121 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6181 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     6241 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6301 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6361 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6421 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     6481 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     6541 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     6601 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     6661 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     6721 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     6781 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     6841 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     6901 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     6961 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7021 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7081 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7141 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7201 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7261 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7321 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     7381 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7441 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     7501 agtgccacct gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag
     7561 cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga
     7621 aagcgctatt ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc
     7681 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
     7741 cgcgagagcg ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc
     7801 atcccgagag cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc
     7861 gctctataat gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa
     7921 ggctactttg gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt
     7981 actgattact agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat
     8041 tctataccga tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct
     8101 tcattggtca gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg
     8161 aaatgtttac attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt
     8221 ttttgtctaa agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg
     8281 caagttcaag gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata
     8341 tagcaaagag atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc
     8401 tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt
     8461 ttcaaaagcg ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact
     8521 tcaaagcgtt tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc
     8581 tcactgttca cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga
     8641 acggcatagt gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg
     8701 aaaggtagtc tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc
     8761 cttcagcact accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat
     8821 ccttcaatgc tatcatttcc tttgatattg gatcatacta agaaaccatt attatcatga
     8881 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
