LOCUS       40924_10726        8888 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCIB2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8888)
  AUTHORS   Dequard-Chablat M, Nguyen T-T., Contamine V, Hermann-Le Denmat S, 
            Malagnac F.
  TITLE     Efficient tools to target DNA to Podospora anserina
  JOURNAL   Fungal Genet Rep 59, 21-25 (2012)
REFERENCE   2  (bases 1 to 8888)
  AUTHORS   Dequard-Chablat M, Nguyen T-T., Contamine V, Hermann-Le Denmat S, 
            Malagnac F.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2012) IGM, UPS XI, CNRS-UMR 8621, 15 rue Georges 
            Clemenceau, Orsay, Ile de France 91405, France
REFERENCE   3  (bases 1 to 8888)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8888)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Rep 59, 21-25 (2012)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-JUN-2012) IGM, UPS XI, CNRS-UMR 8621, 15 rue Georges Clemenceau,
            Orsay, Ile de France 91405, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        196..416
                     /label=URA3 promoter
     CDS             417..1217
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     1951..1967
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1977..1995
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1995..2002
                     /label=modified AscI restriction site
                     /note="modified AscI restriction site"
     5'UTR           2003..2919
                     /gene="Pa_2_3690"
     gene            2003..2919
                     /gene="Pa_2_3690"
                     /label=Pa_2_3690
                     /note="from Podospora anserina chromosome 2"
     misc_feature    2920..2927
                     /label=modified SwaI restriction site
                     /note="modified SwaI restriction site"
     regulatory      2928..3263
                     /gene="GPD"
                     /gene_synonym="Pa_3_5110"
                     /regulatory_class="promoter"
     gene            2928..3263
                     /gene="GPD"
                     /gene_synonym="Pa_3_5110"
                     /label=GPD
     CDS             3270..3833
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     regulatory      3886..4275
                     /gene="AS1"
                     /gene_synonym="Pa_1_16650"
                     /regulatory_class="terminator"
     gene            3886..4275
                     /gene="AS1"
                     /gene_synonym="Pa_1_16650"
                     /label=AS1
     misc_feature    4276..4283
                     /label=modified PacI restriction site
                     /note="modified PacI restriction site"
     3'UTR           4283..5250
                     /gene="Pa_2_3690"
     gene            4283..5250
                     /gene="Pa_2_3690"
                     /label=Pa_2_3690
                     /note="from Podospora anserina chromosome 2"
     misc_feature    5251..5258
                     /label=modified AscI restriction site
                     /note="modified AscI restriction site"
     promoter        complement(5286..5304)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5325..5341)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5349..5365)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5373..5403)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5418..5439)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5727..6315)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6489..7346)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7347..7451)
                     /label=AmpR promoter
     rep_origin      complement(7478..8820)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     1981 acgactcact atagggcgcg ccaccggtgt tctcggcatc tttctgatat ctccgggagg
     2041 gtgtgctcgc tggacaatca gatatcgaat cccactacta acctggtcgg tgtctcagct
     2101 cagccggctg agttgtatgt cacaagccaa tctatgacaa ccttcacgaa agtaattccc
     2161 cgcctgctcg taatctactt tggcatttcc aaggcccaaa tcccatacat gccggtttcg
     2221 agtcaccatt cacgccattc tcgaaatgtt ccaagtttaa tcaacccaca tggtgatccc
     2281 cgtgatggtc cgcatagtct tggtgtctct gaacctctca ccaaaatcgg caatcttctt
     2341 cagactagcc atggttaatt caccctaggc cgcgaaaggc gcagccacac cttagagcga
     2401 atgggcgaat gaccccactc acacacggcc atcggccgag ttcgaagttg gtgtgtatgg
     2461 catcctgtgg gtggccgtca agaaaacaga caggtcactt ttcactccac aaggcagcat
     2521 ctaagcggta atcatcacta gggaactttg accggtgact ctgcgtatac ccgaccatcc
     2581 atggcttttt tttgttatta tgtctctcat ggaagatggt gggcatgtaa ggaacaccag
     2641 ctgcggtcat ttggactttt gggatagtat atatcaactg cggcgttgcc gccttttggg
     2701 gacccatcca ctcatctctc tcttgtttcg ttcactctct gttgatcgag ttccacggca
     2761 gttggggaca gacacacaga ccgtctgtgt gcggcctctc tcgtttcggg aagtctatta
     2821 tttgatagga catcgactcg tgtctttcta agccagtcga cacacaaggg cggctcgacg
     2881 gcaaaaacga agaatctcat gctttgatcg ggtcagacga tttaaatctt cccttccact
     2941 tcttcacaca gaccacacac acatcaggta agtcccatcc tcgagtaaga tcaactggaa
     3001 ccccgcacct cctgcctcac cccgcctgca tcggaccttc tcgagcgaca ccagctccca
     3061 cccaatggct gcccggtccc ggactaggtg gccagcctga ggactggaaa aactttatta
     3121 tctcgcgtct ctccctcaaa agctctctct ccatctcctc gtctcttctt ctcttcgaca
     3181 tctcatcttc ctgcaattcc ctggaaacga aacagcttac tcaccatctc tctctctctc
     3241 tctactaaca gcaaaggatc catatgggta ccactcttga cgacacggct taccggtacc
     3301 gcaccagtgt cccgggggac gccgaggcca tcgaggcact ggatgggtcc ttcaccaccg
     3361 acaccgtctt ccgcgtcacc gccaccgggg acggcttcac cctgcgggag gtgccggtgg
     3421 acccgcccct gaccaaggtg ttccccgacg acgaatcgga cgacgaatcg gacgacgggg
     3481 aggacggcga cccggactcc cggacgttcg tcgcgtacgg ggacgacggc gacctggcgg
     3541 gcttcgtggt cgtctcgtac tccggctgga accgccggct gaccgtcgag gacatcgagg
     3601 tcgccccgga gcaccggggg cacggggtcg ggcgcgcgtt gatggggctc gcgacggagt
     3661 tcgcccgcga gcggggcgcc gggcacctct ggctggaggt caccaacgtc aacgcaccgg
     3721 cgatccacgc gtaccggcgg atggggttca ccctctgcgg cctggacacc gccctgtacg
     3781 acggcaccgc ctcggacggc gagcaggcgc tctacatgag catgccctgc ccctaactgc
     3841 agacgcgtta cgtatcggat ccagaattcg tgattagggc ccggggggct ggatattgtg
     3901 gttcttgcat tcagtctctc atgggttcgg cgaaacggga caacggtctg ctcacgggca
     3961 tgatgcgcta tgcgtgcggt gcctgggcta tcttggtttt agggaaaaga tagtacacaa
     4021 aagagcaatt cgagtgctgg ttccgacaac aacaaacgca caacgcttga tggtgcgctc
     4081 tgaagtgatg aacagagagc tcctggaggg acctcttcat gatgattcat gccaacagaa
     4141 aagggacaga cgtctaggcc gtagttatca gtctgaaatt agaccaataa cacattcctg
     4201 attcgagcaa ttcttgatgc cagcatttgc gtgtattgtg tcccggttct gtttgccttc
     4261 ttgatgcttt gtgccttaat taaccgcatg tttggtgttg gctggctctt tggctcttcg
     4321 gggtaaggga ttctcatcat catccccgtt gtaattgcat tgatcatcat cataggagga
     4381 tacattcata caggttgata ccaggttggc attttctgat ctccatacct cccgtggttt
     4441 atttcttggg ggttcttaca tctatctaca tgtttgcaag atactttttg cttcgcagca
     4501 caaaatacga gattctgcag tttttccccc ttaagaacat ggatgtctgt cgtatgaatt
     4561 atatgtctca gccctctgag ggaattatat gcccgtgaag aggtcaccga gtgttacctt
     4621 tgcacctggc tttttgtaca aaaccatcga gatccccccc gcccccccct atatcttccg
     4681 aaaagccacc cggaatgatg agttgtgcag tgctacatgg gctgagaaca agtgtgtgta
     4741 tgtgatacag aggtccggga tcagatgaaa tcttgaatgt cgagcttgga tcttgatttt
     4801 tatcagaatt tcagatgaac gcggtggtgc tgtgtgtttc cttggtttta cgagagtgat
     4861 tgtaacatat aactcggcca tgctatgatt acgaagacat cctttttctc tcctggtggg
     4921 ggtgggtctg tgtggtgttg tgtggtcacc accaacttgt tttgagtgtg taccaaggtg
     4981 agatggggcg gttcaccgcg tgaaagatgc ttacgacttg cgatgacttg tgacctttga
     5041 atgtgatacg ggattggaat gggtgttagt aaggggaggt gagagagaga gggaggctta
     5101 tcgttatcat cgggacttgt tggtcgtcgg cagtggttgc atttattttt atcgcggcag
     5161 gccggcggcg gcatttggct gatcccggca tccgatggtg agtttgctgg ccccactgcc
     5221 cgctgtcctt gtccttggtt ccctgtctgc ggcgcgccac cgcggtggag ctccagcttt
     5281 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
     5341 gtgtgaaatt gttatccgct cacaattcca cacaacatag gagccggaag cataaagtgt
     5401 aaagcctggg gtgcctaatg agtgaggtaa ctcacattaa ttgcgttgcg ctcactgccc
     5461 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     5521 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5581 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5641 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5701 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5761 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5821 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5881 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5941 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6001 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6061 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6121 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     6181 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6241 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6301 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6361 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6421 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6481 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6541 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6601 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6661 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6721 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6781 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6841 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     6901 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6961 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7021 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7081 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7141 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7201 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7261 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7321 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7381 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7441 ggggttccgc gcacatttcc ccgaaaagtg ccacctgaac gaagcatctg tgcttcattt
     7501 tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat
     7561 ttttacagaa cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc
     7621 atttttgtaa aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc
     7681 tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat
     7741 acttcttttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct
     7801 tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca
     7861 ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa
     7921 aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt
     7981 caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa
     8041 cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta
     8101 ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac
     8161 tctatgaata gttcttacta caattttttt gtctaaagag taatactaga gataaacata
     8221 aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt
     8281 atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga
     8341 agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa
     8401 agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag
     8461 agaataggaa cttcggaata ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa
     8521 tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc gcacctatat ctgcgtgttg
     8581 cctgtatata tatatacatg agaagaacgg catagtgcgt gtttatgctt aaatgcgtac
     8641 ttatatgcgt ctatttatgt aggatgaaag gtagtctagt acctcctgtg atattatccc
     8701 attccatgcg gggtatcgta tgcttccttc agcactaccc tttagctgtt ctatatgctg
     8761 ccactcctca attggattag tctcatcctt caatgctatc atttcctttg atattggatc
     8821 atactaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     8881 ctttcgtc
//
