LOCUS       40924_10716        3685 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCI-YFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Anderson JC, Voigt CA, Arkin AP.
  TITLE     Environmental signal integration by a modular AND gate
  JOURNAL   Mol. Syst. Biol. 3, 133 (2007)
  PUBMED    17700541
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Salis HM, Mirsky EA, Voigt CA.
  TITLE     Automated design of synthetic ribosome binding sites to control 
            protein expression
  JOURNAL   Nat. Biotechnol. 27 (10), 946-950 (2009)
  PUBMED    19801975
REFERENCE   3  (bases 1 to 3685)
  AUTHORS   Tamsir A, Tabor JJ, Voigt CA.
  TITLE     Robust multicellular computing using genetically encoded NOR gates 
            and chemical 'wires'
  JOURNAL   Nature 469 (7329), 212-215 (2011)
  PUBMED    21150903
REFERENCE   4  (bases 1 to 3685)
  AUTHORS   Moser F, Voigt CA.
  TITLE     Genetic Circuit Performance under Conditions Relevant for Industrial
            Bioreactors
  JOURNAL   Unpublished
REFERENCE   5  (bases 1 to 3685)
  AUTHORS   Anderson JC, Moser F, Tamsir A, Salis H.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JAN-2012) Biological Engineering, MIT, 500 Tech 
            Square, Rm 209D, Cambridge, MA 02139, USA
REFERENCE   6  (bases 1 to 3685)
  TITLE     Direct Submission
REFERENCE   7  (bases 1 to 3685)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Syst. 
            Biol. 3, 133 (2007)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Nat. 
            Biotechnol."; date: "2009"; volume: "27"; issue: "10"; pages: 
            "946-950"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Nature"; 
            date: "2011"; volume: "469"; issue: "7329"; pages: "212-215"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 5; type: "Journal Article"; journalName: "Submitted 
            (06-JAN-2012) Biological Engineering, MIT, 500 Tech Square, Rm 209D,
            Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 6; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..21
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      22..81
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     CDS             complement(242..1054)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(1649..2193)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(2683..2726)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2729..2750
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     regulatory      2751..2799
                     /label=PCI BBa_R0051
                     /note="PCI BBa_R0051"
                     /regulatory_class="promoter"
     regulatory      2808..2819
                     /label=BBa_B0034
                     /note="BBa_B0034"
                     /regulatory_class="ribosome_binding_site"
     RBS             2808..2819
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2826..3542
                     /label=EYFP
                     /note="enhanced YFP"
     terminator      3565..3636
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3652..3679
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct
       61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
      121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatctcg
      181 agtcccgtca agtcagcgta atgctctgcc agtgttacaa ccaattaacc aattctgatt
      241 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
      301 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
      361 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
      421 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
      481 aatccggtga gaatggcaaa agcttatgca tttctttcca gacttgttca acaggccagc
      541 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg
      601 cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat
      661 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt
      721 cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat
      781 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta
      841 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca
      901 actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat
      961 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc
     1021 tcgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt
     1081 aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga
     1141 gattttgaga cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc
     1201 agatcacgca tcttcccgac aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca
     1261 ccaactggtc cacctacaac aaagctctca tcaaccgtgg ctccctcact ttctggctgg
     1321 atgatggggc gattcaggcc tggtatgagt cagcaacacc ttcttcacga ggcagacctc
     1381 agcgctagcg gagtgtatac tggcttacta tgttggcact gatgagggtg tcagtgaagt
     1441 gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat gtgatacagg
     1501 atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg
     1561 gaaatggctt acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg
     1621 gaagtgagag ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc
     1681 acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg
     1741 cgtttcccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt
     1801 cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg
     1861 cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct
     1921 tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag
     1981 cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa
     2041 actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag
     2101 ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag
     2161 caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa ggggtctgac
     2221 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2281 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2341 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2401 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2461 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     2521 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2581 ttatccgcct ccatccagtc tattccatgg tgccacctga cgtctaagaa accattatta
     2641 tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat
     2701 ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag taacaccgtg
     2761 cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa gaggagaaat
     2821 actagatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     2881 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     2941 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     3001 ccaccctcgt gaccaccttc ggctacggcc tgcaatgctt cgcccgctac cccgaccaca
     3061 tgaagctgca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     3121 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     3181 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     3241 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     3301 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     3361 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     3421 accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     3481 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     3541 agtaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     3601 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     3661 tcgggtgggc ctttctgcgt ttata
//
