LOCUS       V016085                 6606 bp    DNA     circular SYN 28-OCT-2020
DEFINITION  Exported.
ACCESSION   V016085
VERSION     V016085
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Declercq M, Biquand E, Karim M, Pietrosemoli N, Jacob Y, Demeret C,
            Barbezange C, van der Werf S.
  TITLE     Influenza A virus co-opts ERI1 exonuclease bound to histone mRNA to
            promote viral transcription
  JOURNAL   Nucleic Acids Res 48 (18), 10428-10440 (2020)
   PUBMED   32960265
REFERENCE   2  (bases 1 to 6606)
  AUTHORS   Declercq M, Biquand E, Demeret C, Karim M, Pietrosemoli N, Jacob Y,
            Demeret C, Van der Werf S, Barbezange C.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2020) National Influenza Centre, Sciensano, Rue
            Juliette Wytsman 14, Brussels 1180, Belgium
REFERENCE   3  (bases 1 to 6606)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res"; date: "2020"; volume: "48"; issue: "18"; pages:
            "10428-10440"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-SEP-2020) National Influenza Centre, Sciensano, Rue Juliette
            Wytsman 14, Brussels 1180, Belgium"
FEATURES             Location/Qualifiers
     source          1..6606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          857..989
                     /label="chimeric intron"
                     /note="chimera between introns from human beta-globin and
                     immunoglobulin heavy chain genes"
     misc_feature    1012..1027
                     /label="sequencing primer"
                     /note="sequencing primer"
     promoter        1034..1052
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1067..3343
                     /gene="PB2"
                     /label="Polymerase basic protein 2"
                     /note="Polymerase basic protein 2 from Influenza A virus
                     (strain A/Wilson-Smith/1933 H1N1). Accession#: P03427"
     misc_feature    3344..3382
                     /label="linker"
                     /note="linker"
     misc_feature    3383..3664
                     /label="Gaussia luciferase domain 1"
                     /note="Gaussia luciferase domain 1"
     polyA_signal    complement(3720..3841)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      4022..4477
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4809..4913
                     /label="AmpR promoter"
     CDS             4914..5771
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5945..6533
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagcctc gagaccatgg aaagaataaa
     1081 agaactaagg aatctaatgt cgcagtctcg cactcgcgag atactcacaa aaaccaccgt
     1141 ggaccatatg gccataatca agaagtacac atcaggaaga caggagaaga acccagcact
     1201 taggatgaaa tggatgatgg caatgaaata tccaattaca gcagacaaga ggataacgga
     1261 aatgattcct gagagaaatg agcagggaca aactttatgg agtaaaatga atgacgccgg
     1321 atcagaccga gtgatggtat cacctctggc tgtgacatgg tggaatagga atggaccagt
     1381 gacaagtaca gttcattatc caaaaatcta caaaacttat tttgaaaaag tcgaaaggtt
     1441 aaaacatgga acctttggcc ctgtccattt tagaaaccaa gtcaaaatac gtcgaagagt
     1501 tgacataaat cctggtcatg cagatctcag tgccaaagag gcacaggatg taatcatgga
     1561 agttgttttc cctaacgaag tgggagccag gatactaaca tcggaatcgc aactaacgac
     1621 aaccaaagag aagaaagaag aactccaggg ttgcaaaatt tctcctctga tggtggcata
     1681 catgttggag agagaactgg tccgcaaaac gagattcctc ccagtggctg gtggaacaag
     1741 cagtgtgtac attgaagtgt tgcatttgac ccaaggaaca tgctgggaac agatgtacac
     1801 tccaggaggg gaggcgagga atgatgatgt tgatcaaagc ttaattattg ctgctagaaa
     1861 catagtaaga agagccacag tatcagcaga tccactagca tctttattgg agatgtgcca
     1921 cagcacgcag attggtggaa taaggatggt aaacatcctt aggcagaacc caacagaaga
     1981 gcaagccgtg gatatttgca aggctgcaat gggactgaga attagctcat ccttcagttt
     2041 tggtggattc acatttaaga gaacaagcgg atcatcagtc aagagagagg aagaggtgct
     2101 tacgggcaat cttcagacat tgaagataag agtgcatgag ggatatgaag agttcacaat
     2161 ggttgggaga agagcaacag ctatactcag aaaagcaacc aggagattga ttcagctgat
     2221 agtgagtggg agagacgaac agtcgattgc cgaagcaata attgtggcca tggtattttc
     2281 acaagaggat tgtatgataa aagcagttag aggtgacctg aatttcgtca atagggcgaa
     2341 tcagcgattg aatcccatgc accaactttt gagacatttt cagaaggatg caaaggtgct
     2401 ctttcaaaat tggggaattg aatccatcga caatgtgatg ggaatgatcg ggatattgcc
     2461 cgacatgact ccaagcaccg agatgtcaat gagaggagtg agaatcagca aaatgggggt
     2521 agatgagtat tccagcgcgg agaagatagt ggtgagcatt gaccgttttt tgagagttag
     2581 ggaccaacgt gggaatgtac tactgtctcc cgaggaggtc agtgaaacac agggaacaga
     2641 gaaactgaca ataacttact catcgtcaat gatgtgggag attaatggtc ctgaatcagt
     2701 gttggtcaat acctatcagt ggattatcag aaactgggaa actgttaaaa ttcagtggtc
     2761 ccagaatcct acaatgctgt acaataaaat ggaatttgag ccatttcagt ctttagttcc
     2821 aaaggccgtt agaggccaat acagtgggtt tgtgagaact ctgttccaac aaatgaggga
     2881 tgtgcttggg acatttgata ccgctcagat aataaaactt cttcccttcg cagccgctcc
     2941 accaaagcaa agtggaatgc agttctcctc attgactata aatgtgaggg gatcaggaat
     3001 gagaatactt gtaaggggca attctccagt attcaactac aacaagacca ctaaaagact
     3061 cacagttctc ggaaaggatg ctggcccttt aactgaagac ccagatgaag gcacagctgg
     3121 agttgagtcc gcagttctga gaggattcct cattctgggc aaagaagaca ggagatatgg
     3181 accagcatta agcataaatg aactgagcaa ccttgcgaaa ggagagaagg ctaatgtgct
     3241 aattgggcaa ggagacgtgg tgttggtaat gaaacggaaa cggaactcta gcatacttac
     3301 tgacagccag acagcgacca aaagaattcg gatggccatc aatgcggccg caggaggagg
     3361 gggctccggc ggagggggat ccaagcccac cgagaacaac gaagacttca acatcgtggc
     3421 cgtggccagc aacttcgcga ccacggatct cgatgctgac cgcgggaagt tgcccggcaa
     3481 gaagctgccg ctggaggtgc tcaaagagat ggaagccaat gcccggaaag ctggctgcac
     3541 caggggctgt ctgatctgcc tgtcccacat caagtgcacg cccaagatga agaagttcat
     3601 cccaggacgc tgccacacct acgaaggcga caaagaatcc gcacagggcg gcataggcta
     3661 atgaacgcgt ggtacctcta gagtcgaccc gggcggccgg ccgcttcgag cagacatgat
     3721 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     3781 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     3841 taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt
     3901 ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccgg gctggcgtaa
     3961 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     4021 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4081 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4141 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4201 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4261 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4321 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     4381 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     4441 aacgcgaatt ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta
     4501 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     4561 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     4621 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     4681 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     4741 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     4801 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4861 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4921 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4981 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5041 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     5101 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     5161 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     5221 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5281 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5341 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5401 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5461 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5521 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5581 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5641 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5701 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5761 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5821 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5881 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5941 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6001 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6061 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     6121 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6181 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6241 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6301 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6361 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6421 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6481 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6541 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atggctcgac
     6601 agatct
//
