LOCUS       V015417                 6600 bp    DNA     circular SYN 28-OCT-2020
DEFINITION  Exported.
ACCESSION   V015417
VERSION     V015417
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Declercq M, Biquand E, Karim M, Pietrosemoli N, Jacob Y, Demeret C,
            Barbezange C, van der Werf S.
  TITLE     Influenza A virus co-opts ERI1 exonuclease bound to histone mRNA to
            promote viral transcription
  JOURNAL   Nucleic Acids Res 48 (18), 10428-10440 (2020)
   PUBMED   32960265
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   Declercq M, Biquand E, Demeret C, Karim M, Pietrosemoli N, Jacob Y,
            Demeret C, Van der Werf S, Barbezange C.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2020) National Influenza Centre, Sciensano, Rue
            Juliette Wytsman 14, Brussels 1180, Belgium
REFERENCE   3  (bases 1 to 6600)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res"; date: "2020"; volume: "48"; issue: "18"; pages:
            "10428-10440"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-SEP-2020) National Influenza Centre, Sciensano, Rue Juliette
            Wytsman 14, Brussels 1180, Belgium"
FEATURES             Location/Qualifiers
     source          1..6600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          857..989
                     /label="chimeric intron"
                     /note="chimera between introns from human beta-globin and
                     immunoglobulin heavy chain genes"
     misc_feature    1012..1027
                     /label="sequencing primer"
                     /note="sequencing primer"
     promoter        1034..1052
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1067..3337
                     /gene="PB1"
                     /label="RNA-directed RNA polymerase catalytic subunit"
                     /note="RNA-directed RNA polymerase catalytic subunit from
                     Influenza A virus (strain A/Wilson-Smith/1933 H1N1).
                     Accession#: P03430"
     misc_feature    3338..3376
                     /label="linker"
                     /note="linker"
     misc_feature    3377..3658
                     /label="Gaussia luciferase domain 1"
                     /note="Gaussia luciferase domain 1"
     polyA_signal    complement(3714..3835)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      4016..4471
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4803..4907
                     /label="AmpR promoter"
     CDS             4908..5765
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5939..6527
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagcctc gagaccatgg atgtcaatcc
     1081 gactttactt ttcttaaaag tgccagcaca aaatgctata agcacaactt tcccttatac
     1141 tggagaccct ccttacagcc atgggacagg aacaggatac accatggata ctgtcaacag
     1201 gacacatcag tactcagaaa ggggaagatg gacaacaaac accgaaactg gagcaccgca
     1261 actcaacccg attgatgggc cactgccaga agacaatgaa ccaagtggtt atgcccaaac
     1321 agattgtgta ttggaagcaa tggccttcct tgaggaatcc catcctggta tctttgagac
     1381 ctcgtgtctt gaaacgatgg aggttgttca gcaaacacga gtggacaagc tgacacaagg
     1441 ccgacagacc tatgactgga ctctaaatag gaaccagcct gctgcaacag cattggccaa
     1501 cacaatagaa gtgttcagat caaatggcct cacggccaat gaatctggaa ggctcataga
     1561 cttccttaag gatgtaatgg agtcaatgaa caaagaagaa atggagatca caactcattt
     1621 tcagagaaag agacgagtga gagacaatat gactaagaaa atggtgacac agagaacaat
     1681 aggtaaaagg aagcagagat tgaacaaaag gagttatcta attagggcat taaccctgaa
     1741 cacaatgacc aaagatgctg agagagggaa gctaaaacgg agagcaattg caaccccagg
     1801 gatgcaaata agggggtttg tatactttgt tgagacacta gcaaggagta tatgtgagaa
     1861 acttgaacaa tcaggattgc cagttggagg caatgagaag aaagcaaagt tggcaaatgt
     1921 tgtaaggaag atgatgacca attctcagga cactgaaatt tctttcacca tcactggaga
     1981 taacaccaaa tggaacgaaa atcagaaccc tcggatgttt ttggccatga tcacatatat
     2041 aaccagaaat cagcccgaat ggttcagaaa tgttctaagt attgctccaa taatgttctc
     2101 aaacaaaatg gcgagactgg gaaaggggta catgtttgag agcaagagta tgaaacttag
     2161 aactcaaata cctgcagaaa tgctagcaag catcgatttg aaatacttca atgattcaac
     2221 tagaaagaag attgaaaaaa tccggccgct cttaatagat gggactgcat cattgagccc
     2281 tggaatgatg atgggcatgt tcaatatgtt aagtactgta ttaggcgtct ccatcctgaa
     2341 tcttggacaa aagagacaca ccaagactac ttactggtgg gatggtcttc aatcttctga
     2401 tgattttgct ctgattgtga atgcacccaa tcatgaaggg attcaagccg gagtcaacag
     2461 gttttatcga acctgtaagc tacttggaat taatatgagc aagaaaaagt cttacataaa
     2521 cagaacaggt acatttgaat tcacaagttt tttctatcgt tatgggtttg ttgccaattt
     2581 cagcatggag cttcccagct ttggggtgtc tgggatcaac gagtctgcgg acatgagtat
     2641 tggagttact gtcatcaaaa acaatatgat aaacaatgat cttggtccag caaccgctca
     2701 aatggccctt cagctgttca tcaaagatta caggtacacg taccggtgcc atagaggtga
     2761 cacacaaata caaacccgaa gatcatttga aataaagaaa ctgtgggagc aaacccattc
     2821 caaagctgga ctgctggtct ccgacggagg cccaaattta tacaacatta gaaatctcca
     2881 cattcctgaa gtctgcttga aatgggaatt aatggatgag gattaccagg ggcgtttatg
     2941 caacccactg aacccatttg tcaaccataa agacattgaa tcagtgaaca atgcagtgat
     3001 aatgccagca catggtccag ccaaaaacat ggagtatgat gctgttgcaa caacacattc
     3061 ctggatcccc aaaagaaatc gatccatctt gaatacaagc caaagaggaa tacttgaaga
     3121 tgaacaaatg taccaaaagt gctgcaactt atttgaaaaa ttcttcccca gcagttcata
     3181 cagaagacca gtcgggatat ccagtatggt ggaggctatg gtttccagag cccgaattga
     3241 tgcacgaatt gatttcgaat ctggaaggat aaagaaagag gagttcactg agatcatgaa
     3301 gatctgttcc accattgaag agctcagacg gcaaaaagcg gccgcaggag gagggggctc
     3361 cggcggaggg ggatccaagc ccaccgagaa caacgaagac ttcaacatcg tggccgtggc
     3421 cagcaacttc gcgaccacgg atctcgatgc tgaccgcggg aagttgcccg gcaagaagct
     3481 gccgctggag gtgctcaaag agatggaagc caatgcccgg aaagctggct gcaccagggg
     3541 ctgtctgatc tgcctgtccc acatcaagtg cacgcccaag atgaagaagt tcatcccagg
     3601 acgctgccac acctacgaag gcgacaaaga atccgcacag ggcggcatag gctaatgaac
     3661 gcgtggtacc tctagagtcg acccgggcgg ccggccgctt cgagcagaca tgataagata
     3721 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     3781 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     3841 caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag
     3901 caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgggctggc gtaatagcga
     3961 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg
     4021 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4081 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4141 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4201 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     4261 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4321 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     4381 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     4441 aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc
     4501 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     4561 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     4621 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     4681 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     4741 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     4801 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4861 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4921 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4981 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5041 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5101 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5161 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5221 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5281 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5341 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5401 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5461 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5521 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5581 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5641 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5701 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5761 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     5821 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     5881 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5941 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6001 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6061 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6121 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6181 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6241 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6301 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6361 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6421 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6481 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6541 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatggct cgacagatct
//
