LOCUS       V015299                 6477 bp    DNA     circular SYN 28-OCT-2020
DEFINITION  Exported.
ACCESSION   V015299
VERSION     V015299
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Declercq M, Biquand E, Karim M, Pietrosemoli N, Jacob Y, Demeret C,
            Barbezange C, van der Werf S.
  TITLE     Influenza A virus co-opts ERI1 exonuclease bound to histone mRNA to
            promote viral transcription
  JOURNAL   Nucleic Acids Res 48 (18), 10428-10440 (2020)
   PUBMED   32960265
REFERENCE   2  (bases 1 to 6477)
  AUTHORS   Declercq M, Biquand E, Demeret C, Karim M, Pietrosemoli N, Jacob Y,
            Demeret C, Van der Werf S, Barbezange C.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2020) National Influenza Centre, Sciensano, Rue
            Juliette Wytsman 14, Brussels 1180, Belgium
REFERENCE   3  (bases 1 to 6477)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res"; date: "2020"; volume: "48"; issue: "18"; pages:
            "10428-10440"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-SEP-2020) National Influenza Centre, Sciensano, Rue Juliette
            Wytsman 14, Brussels 1180, Belgium"
FEATURES             Location/Qualifiers
     source          1..6477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          857..989
                     /label="chimeric intron"
                     /note="chimera between introns from human beta-globin and
                     immunoglobulin heavy chain genes"
     misc_feature    1012..1027
                     /label="sequencing primer"
                     /note="sequencing primer"
     promoter        1034..1052
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1067..3214
                     /gene="PA"
                     /label="Polymerase acidic protein"
                     /note="Polymerase acidic protein from Influenza A virus
                     (strain A/Wilson-Smith/1933 H1N1). Accession#: P15659"
     misc_feature    3215..3253
                     /label="linker"
                     /note="linker"
     misc_feature    3254..3535
                     /label="Gaussia luciferase domain 1"
                     /note="Gaussia luciferase domain 1"
     polyA_signal    complement(3591..3712)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      3893..4348
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4680..4784
                     /label="AmpR promoter"
     CDS             4785..5642
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5816..6404
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagcctc gagaccatgg aagattttgt
     1081 gcgacaatgc ttcaatccga tgattgtcga gcttgcggaa aaggcaatga aagagtatgg
     1141 agaggacctg aaaatcgaaa caaacaaatt tgcagcaata tgcactcact tggaagtgtg
     1201 cttcatgtat tcagattttc acttcatcga tgagcaaggc gagtcaatag tcgtagaact
     1261 tggcgatcca aatgcacttt tgaagcacag atttgaaata atcgagggaa gagatcgcac
     1321 aatagcctgg acagtaataa acagtatttg caacactaca ggggctgaga aaccaaagtt
     1381 tctaccagat ttgtatgatt acaagaagaa tagattcatc gaaattggag taacaaggag
     1441 agaagttcac atatactatc tggaaaaggc caataaaatt aaatctgaga agacacacat
     1501 ccacattttc tcattcactg gggaggaaat ggccacaaag gccgactaca ctctcgatga
     1561 agaaagcagg gctaggatca aaaccaggct attcaccata agacaagaaa tggctagcag
     1621 aggcctctgg gattcctttc gtcagtccga gagaggcgaa gagacaattg aagaaagatt
     1681 tgaaatcaca ggaacaatgc gcaagcttgc cgaccaaagt ctcccgccaa acttctccag
     1741 ccttgaaaat tttagagcct atgtggatgg attcgaaccg aacggctaca ttgagggcaa
     1801 gctttctcaa atgtccaaag aagtaaatgc tagaattgaa ccttttttga aatcaacacc
     1861 acgaccactt agacttccgg atgggcctcc ctgttctcag cggtccaaat tcctgctgat
     1921 ggatgcctta aaattaagca ttgaggaccc aagtcatgag ggagagggga taccgctata
     1981 tgatgcaatc aaatgcatga gaacattctt tggatggaag gaacccaatg ttgttaaacc
     2041 acacgaaaag ggaataaatc caaattatct tctgtcatgg aagcaagtac tggcagaact
     2101 gcaggacatt gagaatgagg agaaaattcc aaggactaaa aatatgaaga aaacgagtca
     2161 gttaaagtgg gcacttggtg agaacatggc accagaaaag gtagactttg acgattgtaa
     2221 agatgtaggc gatttgaagc aatatgatag tgatgaacca gaattgaggt cgcttgcaag
     2281 ttggattcag aatgagttca acaaggcatg tgaactgacc gattcaagct ggatagagct
     2341 cgatgagatt ggagaagatg cggctccaat tgaacacatt gcaagcatga gaaggaatta
     2401 tttcacagca gaggtgtctc attgcagagc cacagaatac ataatgaagg gggtgtacat
     2461 caatactgcc ttgcttaatg catcctgtgc agcaatggat gatttccaat tgattccaat
     2521 gataagcaag tgtagaacta aggagggaag gcgaaagacc aatttgtacg gtttcatcat
     2581 aaaaggaaga tcccacttaa ggaatgacac cgatgtggta aactttgtga gcatggagtt
     2641 ttccctcact gacccaagac ttgaaccaca caaatgggag aagtactgtg ttcttgaggt
     2701 aggagatatg cttctaagaa gtgccatagg ccatgtgtca aggcctatgt tcttgtatgt
     2761 gaggacaaat ggaacctcaa aaattaaaat gaaatggggg atggaaatga ggcgttgcct
     2821 ccttcagtca cttcaacaaa tcgagagtat gattgaagct gagtcctctg tcaaggagaa
     2881 agacatgacc aaagagttct ttgaaaacaa atcagaaaca tggcccgttg gagagtcccc
     2941 caaaggagtg gaggaaggtt ccattgggaa ggtctgcaga actttattgg caaagtcggt
     3001 attcaacagc ttgtatgcat ctccacaact ggaaggattt tcagctgaat caagaaaact
     3061 gcttcttatc gttcaggctc ttagggacaa cctggaacct gggacctttg atcttggggg
     3121 gctatatgaa gcaattgagg agtgcctgat taatgatccc tgggttttgc ttaatgcttc
     3181 ttggttcaac tccttcctca cacatgcatt gagagcggcc gcaggaggag ggggctccgg
     3241 cggaggggga tccaagccca ccgagaacaa cgaagacttc aacatcgtgg ccgtggccag
     3301 caacttcgcg accacggatc tcgatgctga ccgcgggaag ttgcccggca agaagctgcc
     3361 gctggaggtg ctcaaagaga tggaagccaa tgcccggaaa gctggctgca ccaggggctg
     3421 tctgatctgc ctgtcccaca tcaagtgcac gcccaagatg aagaagttca tcccaggacg
     3481 ctgccacacc tacgaaggcg acaaagaatc cgcacagggc ggcataggct aatgaacgcg
     3541 tggtacctct agagtcgacc cgggcggccg gccgcttcga gcagacatga taagatacat
     3601 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     3661 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     3721 caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt tttaaagcaa
     3781 gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg ggctggcgta atagcgaaga
     3841 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc
     3901 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3961 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4021 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4081 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     4141 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4201 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     4261 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4321 tttaacaaaa tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt
     4381 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     4441 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     4501 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     4561 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     4621 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     4681 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     4741 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     4801 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     4861 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     4921 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     4981 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5041 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5101 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5161 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5221 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5281 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5341 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5401 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     5461 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     5521 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     5581 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     5641 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     5701 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5761 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5821 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5881 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5941 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     6001 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6061 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6121 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6181 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6241 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6301 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6361 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6421 cctttttacg gttcctggcc ttttgctggc cttttgctca catggctcga cagatct
//
