LOCUS       V016449                 5085 bp    DNA     circular SYN 28-OCT-2020
DEFINITION  Exported.
ACCESSION   V016449
VERSION     V016449
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Declercq M, Biquand E, Karim M, Pietrosemoli N, Jacob Y, Demeret C,
            Barbezange C, van der Werf S.
  TITLE     Influenza A virus co-opts ERI1 exonuclease bound to histone mRNA to
            promote viral transcription
  JOURNAL   Nucleic Acids Res 48 (18), 10428-10440 (2020)
   PUBMED   32960265
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Declercq M, Biquand E, Demeret C, Karim M, Pietrosemoli N, Jacob Y,
            Demeret C, Van der Werf S, Barbezange C.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2020) National Influenza Centre, Sciensano, Rue
            Juliette Wytsman 14, Brussels 1180, Belgium
REFERENCE   3  (bases 1 to 5085)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res"; date: "2020"; volume: "48"; issue: "18"; pages:
            "10428-10440"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-SEP-2020) National Influenza Centre, Sciensano, Rue Juliette
            Wytsman 14, Brussels 1180, Belgium"
FEATURES             Location/Qualifiers
     source          1..5085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          857..989
                     /label="chimeric intron"
                     /note="chimera between introns from human beta-globin and
                     immunoglobulin heavy chain genes"
     misc_feature    1012..1027
                     /label="sequencing primer"
                     /note="sequencing primer"
     promoter        1034..1052
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1067..1355
                     /label="Gaussia luciferase domain 1"
                     /note="Gaussia luciferase domain 1"
     misc_feature    1356..1393
                     /label="linker"
                     /note="linker"
     CDS             1394..2149
                     /gene="M"
                     /label="Matrix protein 1"
                     /note="Matrix protein 1 from Influenza A virus (strain
                     A/Wilson-Smith/1933 H1N1). Accession#: P05777"
     polyA_signal    complement(2199..2320)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      2501..2956
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3288..3392
                     /label="AmpR promoter"
     CDS             3393..4250
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4424..5012
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagcctc gagaccatgg ggatctccaa
     1081 gcccaccgag aacaacgaag acttcaacat cgtggccgtg gccagcaact tcgcgaccac
     1141 ggatctcgat gctgaccgcg ggaagttgcc cggcaagaag ctgccgctgg aggtgctcaa
     1201 agagatggaa gccaatgccc ggaaagctgg ctgcaccagg ggctgtctga tctgcctgtc
     1261 ccacatcaag tgcacgccca agatgaagaa gttcatccca ggacgctgcc acacctacga
     1321 aggcgacaaa gagtccgcac agggcggcat aggcggagga gggggctccg gaggaggggg
     1381 cgcggccgct accatgagtc ttctaaccga ggtcgaaact tacgttctct ctatcgtccc
     1441 gtcaggcccc ctcaaagccg agatcgcaca gagacttgaa gatgtctttg cagggaagaa
     1501 caccgatctt gaggttctca tggaatggct aaagacaaga ccaatcctgt cacctctgac
     1561 taaggggatt ttaggatttg tgttcacgct caccgtgccc agtgagcggg gactgcagcg
     1621 tagacgcttt gtccaaaatg ctcttaatgg gaacggagat ccaaataaca tggacaaagc
     1681 agttaaactg tataggaagc ttaagaggga gataacattc catggggcca aagaaatagc
     1741 actcagttat tctgctggtg cacttgccag ttgtatgggc ctcatataca acaggatggg
     1801 ggctgtgacc actgaagtgg catttggcct ggtatgcgca acctgtgaac agattgctga
     1861 ctcccagcat cggtctcata ggcaaatggt gacagcaacc aatccactaa tcagacatga
     1921 gaacagaatg gttctagcca gcactacagc taaggctatg gagcaaatgg ctggatcgag
     1981 tgagcaagca gcagaggcca tggatattgc tagtcaggcc aggcaaatgg tgcaggcgat
     2041 gagaaccatt gggactcatc ctagctccag tgctggtcta aaagatgatc ttcttgaaaa
     2101 tttgcaggcc tatcagaaac gaatgggggt gcagatgcaa cgattcaagt gatagtctag
     2161 agtcgacccg ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg
     2221 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     2281 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     2341 ttttatgttt caggttcagg gggagatgtg ggaggttttt taaagcaagt aaaacctcta
     2401 caaatgtggt aaaatcgata aggatccggg ctggcgtaat agcgaagagg cccgcaccga
     2461 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca
     2521 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     2581 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     2641 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     2701 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     2761 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     2821 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     2881 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     2941 ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     3001 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     3061 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     3121 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     3181 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     3241 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     3301 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     3361 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     3421 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     3481 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     3541 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     3601 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     3661 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     3721 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     3781 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     3841 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     3901 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     3961 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     4021 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     4081 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     4141 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     4201 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     4261 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     4321 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     4381 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4441 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4501 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     4561 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4621 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4681 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4741 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4801 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4861 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4921 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4981 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5041 tcctggcctt ttgctggcct tttgctcaca tggctcgaca gatct
//
