LOCUS       V015916                 6606 bp    DNA     circular SYN 28-OCT-2020
DEFINITION  Exported.
ACCESSION   V015916
VERSION     V015916
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Declercq M, Biquand E, Karim M, Pietrosemoli N, Jacob Y, Demeret C,
            Barbezange C, van der Werf S.
  TITLE     Influenza A virus co-opts ERI1 exonuclease bound to histone mRNA to
            promote viral transcription
  JOURNAL   Nucleic Acids Res 48 (18), 10428-10440 (2020)
   PUBMED   32960265
REFERENCE   2  (bases 1 to 6606)
  AUTHORS   Declercq M, Biquand E, Demeret C, Karim M, Pietrosemoli N, Jacob Y,
            Demeret C, Van der Werf S, Barbezange C.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2020) National Influenza Centre, Sciensano, Rue
            Juliette Wytsman 14, Brussels 1180, Belgium
REFERENCE   3  (bases 1 to 6606)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res"; date: "2020"; volume: "48"; issue: "18"; pages:
            "10428-10440"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-SEP-2020) National Influenza Centre, Sciensano, Rue Juliette
            Wytsman 14, Brussels 1180, Belgium"
FEATURES             Location/Qualifiers
     source          1..6606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          857..989
                     /label="chimeric intron"
                     /note="chimera between introns from human beta-globin and
                     immunoglobulin heavy chain genes"
     misc_feature    1012..1027
                     /label="sequencing primer"
                     /note="sequencing primer"
     promoter        1034..1052
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1067..3343
                     /gene="PB2"
                     /label="Polymerase basic protein 2"
                     /note="Polymerase basic protein 2 from Influenza A virus
                     (strain A/Duck/England/1/1956 H11N6). Accession#: Q0A427"
     misc_feature    3344..3382
                     /label="linker"
                     /note="linker"
     misc_feature    3383..3664
                     /label="Gaussia luciferase domain 1"
                     /note="Gaussia luciferase domain 1"
     polyA_signal    complement(3720..3841)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      4022..4477
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4809..4913
                     /label="AmpR promoter"
     CDS             4914..5771
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5945..6533
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagcctc gagaccatgg agagaataaa
     1081 agaactgaga gatctaatgt cgcagtcccg cactcgcgag atactcacta agaccactgt
     1141 ggaccatatg gccataatca aaaagtacac atcaggaagg caagagaaga accccgcact
     1201 cagaatgaag tggatgatgg caatgagata cccaattaca gcagacaaga gaataatgga
     1261 catgattcca gagaggaatg aacaaggaca aaccctctgg agcaaaacaa acgatgctgg
     1321 atcagaccga gtgatggtat cacctctggc cgtaacatgg tggaatagga atggcccaac
     1381 aacaagtaca gttcattacc ctaaggtata taaaacttat ttcgaaaagg tcgaaaggtt
     1441 gaaacatggt accttcggcc ctgtccactt cagaaatcaa gttaaaataa ggaggagagt
     1501 tgatacaaac cctggccatg cagatctcag tgccaaggag gcacaggatg tgattatgga
     1561 agttgttttc ccaaatgaag tgggggcaag aatactgaca tcagagtcac agctggcaat
     1621 aacaaaagag aagaaagaag agctccagga ttgtaaaatt gctcccttga tggtggcgta
     1681 catgctagaa agagaattgg tccgtaaaac aaggttcctc ccagtagccg gcggaacagg
     1741 cagtgtttat attgaagtgt tgcacttaac ccaagggacg tgctgggagc agatgtacac
     1801 tccaggagga gaagtgagaa atgatgatgt tgaccaaagt ttgattatcg ctgctagaaa
     1861 catagtaaga agagcagcag tgtcagcaga cccattagca tctctcttgg aaatgtgcca
     1921 cagcacgcag attggaggag taaggatggt ggacatcctt agacagaatc caactgagga
     1981 acaagccgta gacatatgca aggcagcaat agggttgagg attagctcat ctttcagttt
     2041 tggtgggttc actttcaaaa ggacaagcgg atcatcagtc aagaaagaag aagaagtgct
     2101 aacaggcaac ctccaaactc tgaaaataag agtacatgaa gggtatgaag aattcacaat
     2161 ggttgggaga agagcaacag ctattctcag aaaggcaacc aggagattga tccagttgat
     2221 agtaagcggg agagacgagc agtcaattgc tgaggcaata attgtggcca tggtattctc
     2281 acaagaggat tgcatgatca aggcagttag gggcgatctg aactttgtca atagggcaaa
     2341 ccagcgacta aaccccatgc accaactctt gaggcatttc caaaaagatg caaaagtgct
     2401 tttccagaac tggggaattg aatccatcga caatgtgatg ggaatgatcg gaatactgcc
     2461 cgacatgacc ccaagcacgg agatgtcgct gagagggata agagtcagca aaatgggagt
     2521 agatgaatac tccagcacgg agagagtggt agtgagtatt gaccgatttt taagggttag
     2581 agatcaaaga gggaacgtac tattgtctcc cgaagaagtc agtgaaacgc aaggaactga
     2641 gaagttgaca ataacttatt cgtcatcaat gatgtgggag atcaatggcc ctgagtcagt
     2701 gctagtcaac acttatcaat ggataatcag gaactgggaa attgtgaaaa ttcaatggtc
     2761 acaagatccc acaatgttat acaacaaaat ggaatttgaa ccatttcagt ctcttgtccc
     2821 taaggcaacc agaagccggt acagtggatt cgtaaggaca ctgttccagc aaatgcggga
     2881 tgtgcttggg acatttgaca ctgtccaaat aataaaactt ctcccctttg ctgctgctcc
     2941 accagaacag agtaggatgc aattttcctc attgactgtg aatgtaagag gatcagggtt
     3001 gaggatactg gtaagaggca attctccagt attcaattac aacaaggcaa ccaaacgact
     3061 tacagttctt ggaaaggatg caggtgcatt gactgaagat ccagatgaag gcacatctgg
     3121 ggtggagtct gctgtcctga gaggatttct cattttgggc aaagaagaca agagatatgg
     3181 cccagcatta ggcatcaatg aactgagcaa tcttgcaaaa ggagagaaag ctaatgtgct
     3241 aattgggcaa ggggacgtag tgttggtaat gaaacgaaaa cgggactcta gcatacttac
     3301 tgacagccag acagcgacca aaagaattcg gatggccatc aatgcggccg caggaggagg
     3361 gggctccggc ggagggggat ccaagcccac cgagaacaac gaagacttca acatcgtggc
     3421 cgtggccagc aacttcgcga ccacggatct cgatgctgac cgcgggaagt tgcccggcaa
     3481 gaagctgccg ctggaggtgc tcaaagagat ggaagccaat gcccggaaag ctggctgcac
     3541 caggggctgt ctgatctgcc tgtcccacat caagtgcacg cccaagatga agaagttcat
     3601 cccaggacgc tgccacacct acgaaggcga caaagaatcc gcacagggcg gcataggcta
     3661 atgaacgcgt ggtacctcta gagtcgaccc gggcggccgg ccgcttcgag cagacatgat
     3721 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     3781 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     3841 taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt
     3901 ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccgg gctggcgtaa
     3961 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     4021 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4081 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4141 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4201 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4261 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4321 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     4381 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     4441 aacgcgaatt ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta
     4501 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     4561 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     4621 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     4681 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     4741 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     4801 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4861 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4921 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4981 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5041 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     5101 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     5161 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     5221 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5281 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5341 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5401 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5461 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5521 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5581 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5641 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5701 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5761 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5821 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5881 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5941 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6001 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6061 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     6121 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6181 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6241 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6301 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6361 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6421 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6481 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6541 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atggctcgac
     6601 agatct
//
