LOCUS       V016066                 6600 bp    DNA     circular SYN 28-OCT-2020
DEFINITION  Exported.
ACCESSION   V016066
VERSION     V016066
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Declercq M, Biquand E, Karim M, Pietrosemoli N, Jacob Y, Demeret C,
            Barbezange C, van der Werf S.
  TITLE     Influenza A virus co-opts ERI1 exonuclease bound to histone mRNA to
            promote viral transcription
  JOURNAL   Nucleic Acids Res 48 (18), 10428-10440 (2020)
   PUBMED   32960265
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   Declercq M, Biquand E, Demeret C, Karim M, Pietrosemoli N, Jacob Y,
            Demeret C, Van der Werf S, Barbezange C.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2020) National Influenza Centre, Sciensano, Rue
            Juliette Wytsman 14, Brussels 1180, Belgium
REFERENCE   3  (bases 1 to 6600)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res"; date: "2020"; volume: "48"; issue: "18"; pages:
            "10428-10440"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-SEP-2020) National Influenza Centre, Sciensano, Rue Juliette
            Wytsman 14, Brussels 1180, Belgium"
FEATURES             Location/Qualifiers
     source          1..6600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          857..989
                     /label="chimeric intron"
                     /note="chimera between introns from human beta-globin and
                     immunoglobulin heavy chain genes"
     misc_feature    1012..1027
                     /label="sequencing primer"
                     /note="sequencing primer"
     promoter        1034..1052
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1067..3337
                     /gene="PB1"
                     /label="RNA-directed RNA polymerase catalytic subunit"
                     /note="RNA-directed RNA polymerase catalytic subunit from
                     Influenza A virus (strain A/Memphis/8/1988 H3N2).
                     Accession#: P16507"
     misc_feature    3338..3376
                     /label="linker"
                     /note="linker"
     misc_feature    3377..3658
                     /label="Gaussia luciferase domain 1"
                     /note="Gaussia luciferase domain 1"
     polyA_signal    complement(3714..3835)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      4016..4471
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4803..4907
                     /label="AmpR promoter"
     CDS             4908..5765
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5939..6527
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagcctc gagaccatgg atgtcaatcc
     1081 gactctactt ttcctaaaaa ttccagcgca aaatgccata agcaccacat tcccttatac
     1141 tggagatcct ccatacagcc atggaacagg aacaggatac accatggaca cagtaaacag
     1201 aacacaccaa tactcagaaa agggaaagtg gacgacaaac acagagactg gtgcacccca
     1261 gctcaacccg attgatggac cactacctga ggataatgaa ccaagtgggt atgcacaaac
     1321 agactgtgtt ctagaggcta tggctttcct tgaagaatcc cacccaggaa tatttgagaa
     1381 ttcatgcctt gaaacaatgg aagttgttca acaaacaagg gtagataaac taactcaagg
     1441 tcgccagact tatgattgga cattaaacag aaatcaaccg gcagcaactg cgttggccaa
     1501 caccatagaa gtctttagat cgaatggcct aacagctaat gagtcaggaa ggctaataga
     1561 tttcttaaag gatgtaatgg aatcaatgaa caaagaggaa atagagataa caacccactt
     1621 tcaaagaaaa aggagagtaa gagacaacat gaccaagaag atggtcacgc aaagaacaat
     1681 agggaagaaa aaacaaagac tgaataagag aggctatcta ataagagcac tgacattaaa
     1741 tacgatgacc aaagatgcag agagaggcaa gctaaaaaga agggctatcg caacacctgg
     1801 gatgcagatt agaggtttcg tatactttgt tgaaacttta gctaggagca tttgcgaaaa
     1861 gcttgaacag tctgggctcc cagtaggggg caatgaaaag aaggccaaac tggcaaatgt
     1921 tgtgagaaag atgatgacta attcacaaga cacagagatt tctttcacaa tcactgggga
     1981 caacactaag tggaatgaaa atcaaaatcc tcgaatgttc ctggcgatga ttacatatat
     2041 caccagaaat caacccgagt ggttcagaaa catcctgagc atggcaccca taatgttctc
     2101 aaacaaaatg gcaagactag ggaaagggta catgttcgag agtaaaagaa tgaagattcg
     2161 aacacaaata ccagcagaaa tgctagcaag cattgacctg aagtacttca atgaatcaac
     2221 aaagaagaaa attgagaaaa taaggcctct tctaatagat ggcacagcat cactgagtcc
     2281 tgggatgatg atgggcatgt tcaacatgct aagtacggtc ttgggagtct cgatactgaa
     2341 tcttggacaa aagaaataca ccaagacaat atactggtgg gatgggctcc aatcatccga
     2401 cgattttgct ctcatagtga atgcaccaaa ccatgaggga atacaagcag gagtggacag
     2461 attctacagg acctgcaagt tagtgggaat caacatgagc aaaaagaagt cctatataaa
     2521 taagacaggg acattcgaat tcacaagctt tttttatcgc tatggatttg tggctaattt
     2581 tagcatggag ctacccagct ttggagtgtc tggagtaaat gaatcagctg acatgagtat
     2641 tggagtaaca gtgataaaga acaacatgat aaacaatgac cttggacctg caacggccca
     2701 gatggctctt caactgttca tcaaagacta cagatacaca tataggtgcc ataggggaga
     2761 cacacaaatt cagacgagaa gatcatttga gttaaagaag ctgtgggatc aaacccaatc
     2821 aaaggtaggg ctattagtat cagatggagg accaaactta tacaatatac ggaatcttca
     2881 cattcctgaa gtctgcttaa aatgggagct aatggatgat gattatcggg gaagactttg
     2941 taatcccctg aatccctttg tcagtcataa agagattgat tctgtaaaca atgctgtggt
     3001 aatgccagcc catggtccag ccaaaagcat ggaatatgat gccgttgcaa ctacacattc
     3061 ctggattccc aagaggaatc gttccattct caacacaagc caaaggggaa ttcttgagga
     3121 tgaacagatg taccagaagt gctgcaatct attcgagaaa tttttcccta gcagttcata
     3181 taggagaccg gttggaattt ctagcatggt ggaggccatg gtgtctaggg cccggattga
     3241 tgccagggtc gacttcgagt ctggacggat cgggaaggaa gagttctctg agatcatgaa
     3301 gatctgttcc accattgaag aactcagacg gcaaaaagcg gccgcaggag gagggggctc
     3361 cggcggaggg ggatccaagc ccaccgagaa caacgaagac ttcaacatcg tggccgtggc
     3421 cagcaacttc gcgaccacgg atctcgatgc tgaccgcggg aagttgcccg gcaagaagct
     3481 gccgctggag gtgctcaaag agatggaagc caatgcccgg aaagctggct gcaccagggg
     3541 ctgtctgatc tgcctgtccc acatcaagtg cacgcccaag atgaagaagt tcatcccagg
     3601 acgctgccac acctacgaag gcgacaaaga atccgcacag ggcggcatag gctaatgaac
     3661 gcgtggtacc tctagagtcg acccgggcgg ccggccgctt cgagcagaca tgataagata
     3721 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     3781 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     3841 caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag
     3901 caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgggctggc gtaatagcga
     3961 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg
     4021 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4081 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4141 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4201 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     4261 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4321 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     4381 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     4441 aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc
     4501 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     4561 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     4621 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     4681 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     4741 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     4801 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4861 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4921 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4981 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5041 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5101 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5161 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5221 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5281 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5341 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5401 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5461 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5521 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5581 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5641 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5701 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5761 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     5821 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     5881 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5941 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6001 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6061 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6121 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6181 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6241 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6301 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6361 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6421 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6481 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6541 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatggct cgacagatct
//
