LOCUS       62056_6365        4986 bp DNA     circular SYN 28-OCT-2020
DEFINITION  Cloning vector pCI-7608NS1-Gluc1, complete sequence.
ACCESSION   MT966974
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Declercq M, Biquand E, Karim M, Pietrosemoli N, Jacob Y, Demeret C, 
            Barbezange C, van der Werf S.
  TITLE     Influenza A virus co-opts ERI1 exonuclease bound to histone mRNA to 
            promote viral transcription
  JOURNAL   Nucleic Acids Res 48 (18), 10428-10440 (2020)
  PUBMED    32960265
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Declercq M, Biquand E, Demeret C, Karim M, Pietrosemoli N, Jacob Y, 
            Demeret C, Van der Werf S, Barbezange C.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2020) National Influenza Centre, Sciensano, Rue 
            Juliette Wytsman 14, Brussels 1180, Belgium
REFERENCE   3  (bases 1 to 4986)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"; date: "2020"; volume: "48"; issue: "18"; pages: 
            "10428-10440"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-SEP-2020) National Influenza Centre, Sciensano, Rue Juliette 
            Wytsman 14, Brussels 1180, Belgium"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    1012..1027
                     /label=sequencing primer
                     /note="sequencing primer"
     promoter        1034..1052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1067..2041
                     /codon_start=1
                     /transl_table=11
                     /product="7608NS1-Gluc1"
                     /label=7608NS1-Gluc1
                     /note="Influenza virus A/Bretagne/7608/2009 NS1 protein
                     fused to Gaussia luciferase domain 1"
                     /protein_id="QOQ50703.1"
                     /translation="MDSNTMSSFQVDCFLWHIRKRFADNGLGDAPFLDRLRRDQKSLKG
                     RGNTLGLDIETATLVGKQIVEWILKEESSETLRMTIASVPTSRYLSDMTLEEMSRDWFM
                     LMPRQKIIGPLCVRLDQAVMEKNIVLKANFSVIFNRLETLILLRAFTEEGAIVGEISPL
                     PSLPGHTYEDVKNAVGVLIGGLEWNGNTVRVSENIQRFAWRNCDENGRPSLPPEQKAAA
                     GGGGSGGGGSKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARK
                     AGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIG"
     misc_feature    1067..1723
                     /label=Influenza virus A/Bretagne/7608/2009 NS1 protein
                     /note="Influenza virus A/Bretagne/7608/2009 NS1 protein"
     misc_feature    1567
                     /label=mutation A>C in splicing site
                     /note="mutation A>C in splicing site"
     misc_feature    1724..1762
                     /label=linker
                     /note="linker"
     misc_feature    1763..2044
                     /label=Gaussia luciferase domain 1
                     /note="Gaussia luciferase domain 1"
     polyA_signal    complement(2100..2221)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      2402..2857
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3189..3293
                     /label=AmpR promoter
     CDS             3294..4151
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4325..4913
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagcctc gagaccatgg actccaacac
     1081 catgtcaagc tttcaggtag actgtttcct ttggcatatc cgcaagcgat ttgcagacaa
     1141 tggattgggt gatgccccat tccttgatcg gctccgccga gatcaaaagt ccttaaaagg
     1201 aagaggcaac acccttggcc tcgatatcga aacagccact cttgttggga aacaaatcgt
     1261 ggaatggatc ttgaaagagg aatccagcga gacacttaga atgacaattg catctgtacc
     1321 tacttcgcgc tacctttctg acatgaccct cgaggaaatg tcacgagact ggttcatgct
     1381 catgcctagg caaaagataa taggccctct ttgcgtgcga ttggaccagg cggtcatgga
     1441 aaagaacata gtactgaaag cgaacttcag tgtaatcttt aaccgattag agaccttgat
     1501 actactaagg gctttcactg aggagggagc aatagttgga gaaatttcac cattaccttc
     1561 tcttcccgga catacttatg aggatgtcaa aaatgcagtt ggggtcctca tcggaggact
     1621 tgaatggaat ggtaacacgg ttcgagtctc tgaaaatata cagagattcg cttggagaaa
     1681 ctgtgatgag aatgggagac cttcactacc tccagagcag aaagcggccg caggaggagg
     1741 gggctccggc ggagggggat ccaagcccac cgagaacaac gaagacttca acatcgtggc
     1801 cgtggccagc aacttcgcga ccacggatct cgatgctgac cgcgggaagt tgcccggcaa
     1861 gaagctgccg ctggaggtgc tcaaagagat ggaagccaat gcccggaaag ctggctgcac
     1921 caggggctgt ctgatctgcc tgtcccacat caagtgcacg cccaagatga agaagttcat
     1981 cccaggacgc tgccacacct acgaaggcga caaagaatcc gcacagggcg gcataggcta
     2041 atgaacgcgt ggtacctcta gagtcgaccc gggcggccgg ccgcttcgag cagacatgat
     2101 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     2161 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     2221 taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt
     2281 ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccgg gctggcgtaa
     2341 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     2401 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     2461 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     2521 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     2581 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     2641 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     2701 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     2761 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     2821 aacgcgaatt ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta
     2881 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     2941 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     3001 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     3061 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     3121 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     3181 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     3241 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     3301 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     3361 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     3421 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     3481 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     3541 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     3601 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     3661 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     3721 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     3781 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     3841 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     3901 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     3961 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     4021 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     4081 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     4141 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     4201 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     4261 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4321 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4381 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4441 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     4501 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     4561 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     4621 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4681 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     4741 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4801 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4861 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4921 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atggctcgac
     4981 agatct
//
