LOCUS       V016156                 5823 bp    DNA     circular SYN 28-OCT-2020
DEFINITION  Exported.
ACCESSION   V016156
VERSION     V016156
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Declercq M, Biquand E, Karim M, Pietrosemoli N, Jacob Y, Demeret C,
            Barbezange C, van der Werf S.
  TITLE     Influenza A virus co-opts ERI1 exonuclease bound to histone mRNA to
            promote viral transcription
  JOURNAL   Nucleic Acids Res 48 (18), 10428-10440 (2020)
   PUBMED   32960265
REFERENCE   2  (bases 1 to 5823)
  AUTHORS   Declercq M, Biquand E, Demeret C, Karim M, Pietrosemoli N, Jacob Y,
            Demeret C, Van der Werf S, Barbezange C.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2020) National Influenza Centre, Sciensano, Rue
            Juliette Wytsman 14, Brussels 1180, Belgium
REFERENCE   3  (bases 1 to 5823)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res"; date: "2020"; volume: "48"; issue: "18"; pages:
            "10428-10440"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-SEP-2020) National Influenza Centre, Sciensano, Rue Juliette
            Wytsman 14, Brussels 1180, Belgium"
FEATURES             Location/Qualifiers
     source          1..5823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          857..989
                     /label="chimeric intron"
                     /note="chimera between introns from human beta-globin and
                     immunoglobulin heavy chain genes"
     misc_feature    1012..1027
                     /label="sequencing primer"
                     /note="sequencing primer"
     promoter        1034..1052
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1067..2560
                     /gene="NP"
                     /label="Nucleoprotein"
                     /note="Nucleoprotein from Influenza A virus (strain A/New
                     Zealand:South Canterbury/35/2000 H1N1). Accession#: Q289M3"
     misc_feature    2561..2599
                     /label="linker"
                     /note="linker"
     misc_feature    2600..2881
                     /label="Gaussia luciferase domain 1"
                     /note="Gaussia luciferase domain 1"
     polyA_signal    complement(2937..3058)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      3239..3694
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4026..4130
                     /label="AmpR promoter"
     CDS             4131..4988
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5162..5750
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagcctc gagaccatgg cgtcccaagg
     1081 caccaaacgg tcttacgaac agatggagac tgatggggaa cgccagaatg caactgaaat
     1141 cagagcatcc gtcggaagaa tgattggtgg aattgggcga ttctacatcc aaatgtgcac
     1201 cgagcttaag ctcaatgatt atgagggacg actgatccag aatagcttaa caatagagag
     1261 aatggtgctc tctgcttttg atgagaggag aaataaatat ctggaagaac atcccagcgc
     1321 ggggaaagat cctaagaaaa ctggaggacc catatacaag agagtagatg gaaagtgggt
     1381 gagggaactc gtcctttatg acaaagaaga aataaggcgg atttggcgcc aagccaacaa
     1441 tggtgatgat gcaacggctg gtttgactca cattatgatc tggcattcta atttgaatga
     1501 tacaacttac cagaggacaa gagctcttgt ccgcaccgga atggatccca ggatgtgctc
     1561 tttgatgcaa ggttcaactc tccctagaag atctggagca gcaggcgctg cagtcaaagg
     1621 agttgggaca atggtattgg agttaatcag gatgatcaaa cgtgggatca atgaccgaaa
     1681 cttctggagg ggtgagaatg gaagaaaaac aaggattgct tatgagagaa tgtgcaacat
     1741 tctcaaagga aaatttcaaa cagctgcaca aaaagcaatg atggatcaag tgagagaaag
     1801 ccggaaccca ggaaatgctg agatcgaaga tctcactttt ctggcacggt ctgcactcat
     1861 attgagagga tcagttgctc acaagtcttg cctgcctgcc tgtgtgtatg gaccagccgt
     1921 agccagtggg tacgacttcg aaaaagaggg atactctttg gtaggagtag accctttcaa
     1981 actgcttcaa accagtcagg tatacagcct aattagacca aacgagaatc ccgcacacaa
     2041 gagccagttg gtgtggatgg catgcaattc tgctgcattt gaagatctaa gagtgtcaag
     2101 cttcatcaga ggaacaagag tacttccaag ggggaagctc tccactagag gagtacaaat
     2161 tgcttcaaat gaaaacatgg atgctattgt atcaagtact cttgaactga gaagcagata
     2221 ctgggccata agaaccagaa gtggagggaa cactaatcaa caaagggcct ctgcgggcca
     2281 aatcagcaca caacctacat tttctgtgca gagaaacctc ccatttgaca aaacaaccat
     2341 catggcagca ttcactggga atacagaggg aagaacatca gacatgaggg cagaaatcat
     2401 aaagatgatg gaaagtgcaa gaccagaaga agtgtccttc caggggcggg gagtctttga
     2461 gctctcggac gaaagggcaa cgaacccgat cgtgccctcc tttgacatga gtaatgaagg
     2521 atcttatttc ttcggagaca atgcagagga gtacgacaat gcggccgcag gaggaggggg
     2581 ctccggcgga gggggatcca agcccaccga gaacaacgaa gacttcaaca tcgtggccgt
     2641 ggccagcaac ttcgcgacca cggatctcga tgctgaccgc gggaagttgc ccggcaagaa
     2701 gctgccgctg gaggtgctca aagagatgga agccaatgcc cggaaagctg gctgcaccag
     2761 gggctgtctg atctgcctgt cccacatcaa gtgcacgccc aagatgaaga agttcatccc
     2821 aggacgctgc cacacctacg aaggcgacaa agaatccgca cagggcggca taggctaatg
     2881 aacgcgtggt acctctagag tcgacccggg cggccggccg cttcgagcag acatgataag
     2941 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     3001 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     3061 caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta
     3121 aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgggct ggcgtaatag
     3181 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggac
     3241 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3301 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     3361 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     3421 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     3481 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     3541 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     3601 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     3661 gcgaatttta acaaaatatt aacgcttaca atttcctgat gcggtatttt ctccttacgc
     3721 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     3781 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     3841 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     3901 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     3961 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     4021 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4081 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4141 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4201 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4261 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     4321 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     4381 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     4441 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     4501 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4561 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4621 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     4681 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4741 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4801 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4861 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4921 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4981 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5041 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5101 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5161 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5221 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5281 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     5341 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5401 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5461 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5521 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5581 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5641 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5701 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5761 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg gctcgacaga
     5821 tct
//
