LOCUS       40924_10691        4956 bp DNA     circular SYN 17-DEC-2018
DEFINITION  BiFC vector pCHYC155, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Gong Z, Lu H.
  TITLE     A new combination of fluorescent protein fragments suitable for 
            green-colored bimolecular fluorescence complementation (BiFC) assays
            in Schizosaccharomyces pombe
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Gong Z, Lu H.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2012) School of Life Science, Fudan University, 
            Room 618, Science Building, Handan Road 220, Shanghai 200433, China
REFERENCE   3  (bases 1 to 4956)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4956)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-DEC-2012) School of Life Science, Fudan University, Room 618, 
            Science Building, Handan Road 220, Shanghai 200433, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        235..253
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    294..344
                     /label=linker
                     /note="linker"
     CDS             345..596
                     /label=VC155
                     /note="C-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
     CDS             597..614
                     /label=6xHis
                     /note="6xHis affinity tag"
     regulatory      624..1116
                     /label=Saccharomyces cerevisiae LEU2 terminator
                     /note="Saccharomyces cerevisiae LEU2 terminator"
                     /regulatory_class="terminator"
     gene            1162..2737
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin
                     resistance"
     promoter        complement(2845..2863)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3121..3709)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3883..4740)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4741..4845)
                     /label=AmpR promoter
ORIGIN
        1 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
       61 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
      121 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tggacatatt
      181 gtcgttagaa cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag
      241 gtgacactat agaacgcggc cgccagctga agcttcccgg tacccccctc gagcgtcctg
      301 cttgcaagat tcctaacgac cttaagcaaa aggtcatgaa ccacgacaaa caaaagaatg
      361 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
      421 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
      481 acctgtccta tcaatctaag ctttcgaaag atcccaacga aaagagagac cacatggtcc
      541 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaacacc
      601 atcaccatca ccattaatct agagaaaatc cttgcttaaa aagattctct ttttttatga
      661 tatttgtaca taaactttat aaatgaaatt cataatagaa acgacacgaa attacaaaat
      721 ggaatatgtt catagggtag acgaaactat atacgcaatc tacatacatt tatcaagaag
      781 gagaaaaagg aggatgtaaa ggaatacagg taagcaaatt gatactaatg gctcaacgtg
      841 ataaggaaaa agaattgcac tttaacatta atattgacaa ggaggagggc accacacaaa
      901 aagttaggtg taacagaaaa tcatgaaact atgattccta atttatatat tggaggattt
      961 tctctaaaaa aaaaaaaata caacaaataa aaaacactca atgacctgac catttgatgg
     1021 agtttaagtc aataccttct tgaaccattt cccataatgg tgaaagttcc ctcaagaatt
     1081 ttactctgtc agaaacggcc ttaacgacgt agtcgaagat ctgtttagct tgccttgtcc
     1141 ccgccgggtc acccggccag cgacatggag gcccagaata ccctccttga cagtcttgac
     1201 gtgcgcagct caggggcatg atgtgactgt cgcccgtaca tttagcccat acatccccat
     1261 gtataatcat ttgcatccat acattttgat ggccgcacgg cgcgaagcaa aaattacggc
     1321 tcctcgctgc agacctgcga gcagggaaac gctcccctca cagacgcgtt gaattgtccc
     1381 cacgccgcgc ccctgtagag aaatataaaa ggttaggatt tgccactgag gttcttcttt
     1441 catatacttc cttttaaaat cttgctagga tacagttctc acatcacatc cgaacataaa
     1501 caaccatggg taaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
     1561 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
     1621 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     1681 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
     1741 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
     1801 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
     1861 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
     1921 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
     1981 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
     2041 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
     2101 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
     2161 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
     2221 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
     2281 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
     2341 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
     2401 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
     2461 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
     2521 gggcaaagga ataatcagta ctgacaataa aaagattctt gttttcaaga acttgtcatt
     2581 tgtatagttt ttttatattg tagttgttct attttaatca aatgttagcg tgatttatat
     2641 tttttttcgc ctcgacatca tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc
     2701 atgcgtcaat cgtatgtgaa tgctggtcgc tatactgctg tcgattcgat actaacgccg
     2761 ccatccagtg tcgaaaacga gctcgaattc atcgatgata tcagatccac tagtggccta
     2821 tgcggccgcg gatctgccgg tctccctata gtgagtcgta ttaatttcga taagccaggt
     2881 taacctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     2941 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3001 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3061 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3121 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3181 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3241 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3301 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3361 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3421 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3481 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3541 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     3601 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3661 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3721 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3781 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3841 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3901 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3961 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4021 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     4081 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     4141 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     4201 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     4261 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     4321 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     4381 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     4441 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     4501 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     4561 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     4621 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     4681 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     4741 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     4801 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     4861 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     4921 tatcacgagg ccctttcgtc tcgcgcgttt cggtga
//
