LOCUS       V008519                14532 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008519
VERSION     V008519
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14532)
  AUTHORS   Scholte FE, Tas A, Martina BE, Cordioli P, Narayanan K, Makino S,
            Snijder EJ, van Hemert MJ.
  TITLE     Characterization of Synthetic Chikungunya Viruses Based on the
            Consensus Sequence of Recent E1-226V Isolates
  JOURNAL   PLoS ONE 8 (8), E71047 (2013)
   PUBMED   23936484
REFERENCE   2  (bases 1 to 14532)
  AUTHORS   Scholte FEM., Tas A, Snijder EJ, van Hemert MJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2012) Medical Microbiology, Leiden University
            Medical Center, Albinusdreef 2, Leiden, Zuid-Holland 2333 ZA, The
            Netherlands
REFERENCE   3  (bases 1 to 14532)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14532)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2013"; volume: "8"; issue: "8"; pages: "E71047"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (07-OCT-2012) Medical Microbiology, Leiden University Medical
            Center, Albinusdreef 2, Leiden, Zuid-Holland 2333 ZA, The
            Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14532
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      410..430
                     /regulatory_class="promoter"
     5'UTR           430..505
     CDS             506..7930
                     /codon_start=1
                     /transl_except=(pos: 6074 .. 6076,  aa: OTHER)
                     /product="nonstructural polyprotein"
                     /label="nonstructural polyprotein"
                     /note="encodes nonstructural proteins 1 through 4"
                     /protein_id="AGN96196.1"
                     /translation="MDPVYVDIDADSAFLKALQRAYPMFEVEPRQVTPNDHANARAFSH
                     LAIKLIEQEIDPDSTILDIGSAPARRMMSDRKYHCVCPMRSAEDPERLANYARKLASAA
                     GKVLDRNISGKIGDLQAVMAVPDKETPTFCLHTDVSCRQRADVAIYQDVYAVHAPTSLY
                     HQAIKGVRVAYWVGFDTTPFMYNAMAGAYPSYSTNWADEQVLKAKNIGLCSTDLTEGRR
                     GKLSIMRGKKLKPCDRVLFSVGSTLYPESRKLLKSWHLPSVFHLKGKLSFTCRCDTVVS
                     CEGYVVKRITMSPGLYGKTTGYAVTHHADGFLMCKTTDTVDGERVSFSVCTYVPATICD
                     QMTGILATEVTPEDAQKLLVGLNQRIVVNGRTQRNMNTMKNYLLPVVAQAFSKWAKECR
                     KDMEDEKLLGVRERTLTCCCLWAFKKQKTHTVYKRPDTQSIQKVQAEFDSFVVPSLWSS
                     GLSIPLRTRIKWLLSKVPKTDLIPYSGDAREARDAEKEAEEEREAELTREALPPLQAAQ
                     EDVQVEIDVEQLEDRAGAGIIETPRGAIKVTAQPTDHVVGEYLVLSPQTVLRSQKLSLI
                     HALAEQVKTCTHNGRAGRYAVEAYDGRVLVPSGYAISPEDFQSLSESATMVYNEREFVN
                     RKLHHIAMHGPALNTDEESYELVRAERTEHEYVYDVDQRRCCKKEEAAGLVLVGDLTNP
                     PYHEFAYEGLKIRPACPYKIAVIGVFGVPGSGKSAIIKNLVTRQDLVTSGKKENCQEIT
                     TDVMRQRGLEISARTVDSLLLNGCNRPVDVLYVDEAFACHSGTLLALIALVRPRQKVVL
                     CGDPKQCGFFNMMQMKVNYNHNICTQVYHKSISRRCTLPVTAIVSSLHYEGKMRTTNEY
                     NKPIVVDTTGSTKPDPGDLVLTCFRGWVKQLQIDYRGYEVMTAAASQGLTRKGVYAVRQ
                     KVNENPLYASTSEHVNVLLTRTEGKLVWKTLSGDPWIKTLQNPPKGNFKATIKEWEVEH
                     ASIMAGICSHQMTFDTFQNKANVCWAKSLVPILETAGIKLNDRQWSQIIQAFKEDKAYS
                     PEVALNEICTRMYGVDLDSGLFSKPLVSVYYADNHWDNRPGGKMFGFNPEAASILERKY
                     PFTKGKWNINKQICVTTRRIEDFNPTTNIIPANRRLPHSLVAEHRPVKGERMEWLVNKI
                     NGHHVLLVSGYNLALPTKRVTWVAPLGVRGADYTYNLELGLPATLGRYDLVVINIHTPF
                     RIHHYQQCVDHAMKLQMLGGDSLRLLKPGGSLLIRAYGYADRTSERVICVLGRKFRSSR
                     ALKPPCVTSNTEMFFLFSNFDNGRRNFTTHVMNNQLNAAFVGQVTRAGCAPSYRVKRMD
                     IAKNDEECVVNAANPRGLPGDGVCKAVYKKWPESFKNSATPVGTAKTVMCGTYPVIHAV
                     GPNFSNYSESEGDRELAAAYREVAKEVTRLGVNSVAIPLLSTGVYSGGKDRLTQSLNHL
                     FTAMDSTDADVVIYCRDKEWEKKISEAIQMRTQVELLDEHISIDCDIVRVHPDSSLAGR
                     KGYSTTEGALYSYLEGTRFHQTAVDMAEIHTMWPKQTEANEQVCLYALGESIESIRQKC
                     PVDDADASSPPKTVPCLCRYAMTPERVTRLRMNHVTSIIVCSSFPLPKYKIEGVQKVKC
                     SKVMLFDHNVPSRVSPREYRSSQESAQEASTITSLTHSQFDLSVDGEILPVPSDLDADA
                     PALEPALDDGATHTLPSTTGNLAAVSDWVMSTVPVAPPRRRRGRNLTVTCDEREGNITP
                     MASVRFFRAELCPVVQETAETRDTAMSLQAPPSTATEPNHPPISFGASSETFPITFGDF
                     NEGEIESLSSELLTFGDFLPGEVDDLTDSDWSTCSDTDDEL*LDRAGGYIFSSDTGPGH
                     LQQKSVRQSVLPVNTLEEVHEEKCYPPKLDEAKEQLLLKKLQESASMANRSRYQSRKVE
                     NMKAAIIQRLKRGCRLYLMSETPKVPTYRTTYPAPVYSPPINVRLSNPESAVAACNEFL
                     ARNYPTVSSYQITDEYDAYLDMVDGSESCLDRATFNPSKLRSYPKQHAYHAPSIRSAVP
                     SPFQNTLQNVLAAATKRNCNVTQMRELPTLDSAVFNVECFKKFACNQEYWEEFAASPIR
                     ITTENLATYVTKLKGPKAAALFAKTHNLLPLQEVPMDRFTVDMKRDVKVTPGTKHTEER
                     PKVQVIQAAEPLATAYLCGIHRELVRRLNAVLLPNVHTLFDMSAEDFDAIIAAHFKPGD
                     TVLETDIASFDKSQDDSLALTALMLLEDLGVDHSLLDLIEAAFGEISSCHLPTGTRFKF
                     GAMMKSGMFLTLFVNTLLNITIASRVLEDRLTKSACAAFIGDDNIIHGVVSDELMAARC
                     ATWMNMEVKIIDAVVSLKAPYFCGGFILHDTVTGTACRVADPLKRLFKLGKPLAAGDEQ
                     DEDRRRALADEVIRWQRTGLIDELEKAVYSRYEVQGISVVVMSMATFASSRSNFEKLRG
                     PVITLYGGPK"
     regulatory      7909..7928
                     /regulatory_class="promoter"
     CDS             7996..11739
                     /note="Structural polyprotein from Chikungunya virus
                     (strain S27-African prototype). Accession#: Q8JUX5"
                     /label="Structural polyprotein"
     3'UTR           11743..12280
     primer_bind     complement(12311..12327)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    12335..12351
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12359..12389)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(12404..12425)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(12713..13301)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(13475..14332)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(14333..14437)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ggcgcgccac agtaatacga
      421 ctcactatta tggctgcgtg agacacacgt agcctaccag tttcttactg ctctactctg
      481 caaagcaaga gattaataac ccatcatgga tcctgtgtac gtggacatag acgctgacag
      541 cgcctttttg aaggccctgc aacgtgcgta ccccatgttt gaggtggaac caaggcaggt
      601 cacaccgaat gaccatgcta atgctagagc gttctcgcat ctagctataa aactaataga
      661 gcaggaaatt gaccccgact caaccatcct ggatatcggc agtgcgccag caaggaggat
      721 gatgtcggac aggaagtacc actgcgtctg cccgatgcgc agtgcggaag atcccgagag
      781 actcgctaat tatgcgagaa agctagcatc tgccgcagga aaagtcctgg acagaaacat
      841 ctctggaaag atcggggact tacaagcagt aatggccgtg ccagacaagg agacgccaac
      901 attctgctta cacacagacg tctcatgtag acagagagca gacgtcgcta tataccaaga
      961 cgtctatgct gtacacgcac ccacgtcgct ataccaccag gcgattaaag gggtccgagt
     1021 ggcgtactgg gttgggttcg acacaacccc gttcatgtac aatgccatgg cgggtgccta
     1081 cccctcatac tcgacaaact gggcagatga gcaggtactg aaggctaaga acataggatt
     1141 atgttcaaca gacctgacgg aaggtagacg aggcaagttg tctattatga gagggaaaaa
     1201 gctaaaaccg tgcgaccgtg tgctgttctc agtagggtca acgctctacc cggaaagccg
     1261 caagctactt aagagctggc acctgccatc ggtgttccat ttaaagggca aactcagctt
     1321 cacatgccgc tgtgatacag tggtttcgtg tgagggctac gtcgttaaga gaataacgat
     1381 gagcccaggc ctttatggaa aaaccacagg gtatgcggta acccaccacg cagacggatt
     1441 cctgatgtgc aagactaccg acacggttga cggcgaaaga gtgtcattct cggtgtgcac
     1501 atacgtgccg gcgaccattt gtgatcaaat gaccggcatc cttgctacag aagtcacgcc
     1561 ggaggatgca cagaagctgt tggtggggct gaaccagaga atagtggtta acggcagaac
     1621 gcaacggaat atgaacacca tgaaaaatta tctgcttccc gtggtcgccc aagccttcag
     1681 taagtgggca aaggagtgcc ggaaagacat ggaagatgaa aaactcctgg gggtcagaga
     1741 aagaacactg acctgctgct gtctatgggc attcaagaag cagaaaacac acacggtcta
     1801 caagaggcct gatacccagt caattcagaa ggttcaggcc gagtttgaca gctttgtggt
     1861 accgagtctg tggtcgtccg ggttgtcaat ccctttgagg actagaatca aatggttgtt
     1921 aagcaaggtg ccaaaaaccg acctgatccc atacagcgga gacgcccgag aagcccggga
     1981 cgcagaaaaa gaagcagagg aagaacgaga agcagaactg actcgcgaag ccctaccacc
     2041 tctacaggca gcacaggaag atgttcaggt cgaaatcgac gtggaacagc ttgaggacag
     2101 agcgggcgca ggaataatag agactccgag aggagctatc aaagttactg cccaaccaac
     2161 agaccacgtc gtgggagagt acctggtact ctccccgcag accgtactac gtagccagaa
     2221 gctcagtctg attcacgctt tggcggagca agtgaagacg tgcacgcaca acggacgagc
     2281 agggaggtat gcggtcgaag cgtacgacgg ccgagtccta gtgccctcag gctatgcaat
     2341 ctcgcctgaa gacttccaga gtctaagcga aagcgcaacg atggtgtata acgaaagaga
     2401 gttcgtaaac agaaagctac accatattgc gatgcacgga ccagccctga acaccgacga
     2461 agagtcgtat gagctggtga gggcagagag gacagaacac gagtacgtct acgacgtgga
     2521 tcagagaaga tgctgtaaga aggaagaagc cgcaggactg gtactggtgg gcgacttgac
     2581 taatccgccc taccacgaat tcgcatatga agggctaaaa atccgccctg cctgcccata
     2641 caaaattgca gtcataggag tcttcggagt accgggatct ggcaagtcag ctattatcaa
     2701 gaacctagtt accaggcagg acctggtgac tagcggaaag aaagaaaact gccaagaaat
     2761 caccaccgac gtgatgagac agagaggtct agagatatct gcacgtacgg ttgactcgct
     2821 gctcttgaat ggatgcaaca gaccagtcga cgtgttgtac gtagacgagg cgtttgcgtg
     2881 ccactctgga acgctacttg ctttgatcgc cttggtgaga ccaaggcaga aagttgtact
     2941 ttgtggtgac ccgaagcagt gcggcttctt caatatgatg cagatgaaag tcaactataa
     3001 tcacaacatc tgcacccaag tgtaccacaa aagtatctcc aggcggtgta cactgcctgt
     3061 gaccgccatt gtgtcatcgt tgcattacga aggcaaaatg cgcactacga atgagtacaa
     3121 caagccgatt gtagtggaca ctacaggctc aacaaaacct gaccctggag acctcgtgtt
     3181 aacgtgcttc agagggtggg ttaaacaact gcaaattgac tatcgtggat acgaggtcat
     3241 gacagcagcc gcatcccaag ggttaaccag aaaaggagtt tacgcagtta gacaaaaagt
     3301 taatgaaaac ccgctctatg catcaacgtc agagcacgtc aacgtactcc taacgcgtac
     3361 ggaaggtaaa ctggtatgga agacactttc cggcgacccg tggataaaga cgctgcagaa
     3421 cccaccgaaa ggaaacttca aagcaactat taaggagtgg gaggtggagc atgcatcaat
     3481 aatggcgggc atctgcagtc accaaatgac cttcgataca ttccaaaata aagccaacgt
     3541 ttgttgggct aagagcttgg tccctatcct cgaaacagcg gggataaaac taaatgatag
     3601 gcagtggtct cagataattc aagccttcaa agaagacaaa gcatactcac ctgaagtagc
     3661 cctgaatgaa atatgtacgc gcatgtatgg ggtggatcta gacagcgggc tattttctaa
     3721 accgttggtg tctgtgtatt acgcggataa ccactgggat aataggcctg gagggaaaat
     3781 gttcggattt aaccccgagg cagcatccat tctagaaaga aagtatccat tcacaaaagg
     3841 gaagtggaac atcaacaagc agatctgcgt gactaccagg aggatagaag actttaaccc
     3901 taccaccaac atcataccgg ccaacaggag actaccacac tcattagtgg ccgaacaccg
     3961 cccagtaaaa ggggaaagaa tggaatggct ggttaacaag ataaacggcc accacgtgct
     4021 cctggtcagt ggctataacc ttgcactgcc tactaagaga gtcacttggg tagcgccgtt
     4081 aggtgtccgc ggagcggact acacatacaa cctagagttg ggtctgccag caacgcttgg
     4141 taggtatgac cttgtggtca taaacatcca cacacctttt cgcatacacc attaccaaca
     4201 gtgcgtcgac cacgcaatga aactgcaaat gctcgggggt gactcattga gactgctcaa
     4261 accgggcggc tctctattga tcagagcata tggttacgca gatagaacca gtgaacgagt
     4321 catctgcgta ttgggacgca agtttagatc gtctagagcg ttgaaaccac catgtgtcac
     4381 cagcaacact gagatgtttt tcctattcag caactttgac aatggcagaa ggaatttcac
     4441 aactcatgtc atgaacaatc aactgaatgc agccttcgta ggacaggtca cccgagcagg
     4501 atgtgcaccg tcgtaccggg taaaacgcat ggacatcgcg aagaacgatg aagagtgcgt
     4561 agtcaacgcc gctaaccctc gcgggttacc gggtgacggt gtttgcaagg cagtatacaa
     4621 aaaatggccg gagtccttta agaacagtgc aacaccagtg ggaaccgcaa aaacagttat
     4681 gtgcggtacg tatccagtaa tccacgctgt tggaccaaac ttctctaatt attcggagtc
     4741 tgaaggggac cgggaattgg cagctgccta tcgagaagtc gcaaaggaag taactaggct
     4801 gggagtaaat agtgtagcta tacctctcct ctccacaggt gtatactcag gagggaaaga
     4861 caggctgacc cagtcactga accacctctt tacagccatg gactcgacgg atgcagacgt
     4921 ggtcatctac tgccgcgaca aagaatggga gaagaaaata tctgaggcca tacagatgcg
     4981 gacccaagta gagctgctgg atgagcacat ctccatagac tgcgatattg ttcgcgtgca
     5041 ccctgacagc agcttggcag gcagaaaagg atacagcacc acggaaggcg cactgtactc
     5101 atatctagaa gggacccgtt ttcatcagac ggctgtggat atggcggaga tacatactat
     5161 gtggccaaag caaacagagg ccaatgagca agtctgccta tatgccctgg gggaaagtat
     5221 tgaatcgatc aggcagaaat gcccggtgga tgatgcagac gcatcatctc cccccaaaac
     5281 tgtcccgtgc ctttgccgtt acgctatgac tccagaacgc gtcacccggc ttcgcatgaa
     5341 ccacgtcaca agcataattg tgtgttcttc gtttcccctc ccaaagtaca aaatagaagg
     5401 agtgcaaaaa gtcaaatgct ctaaggtaat gctatttgac cacaacgtgc catcgcgcgt
     5461 aagtccaagg gaatatagat cttcccagga gtctgcacag gaggcgagta caatcacgtc
     5521 actgacgcat agtcaattcg acctaagcgt tgatggcgag atactgcccg tcccgtcaga
     5581 cctggatgct gacgccccag ccctagaacc agcactagac gacggggcga cacacacgct
     5641 gccatccaca accggaaacc ttgcggccgt gtctgactgg gtaatgagca ccgtacctgt
     5701 cgcgccgccc agaagaaggc gagggagaaa cctgactgtg acatgtgacg agagagaagg
     5761 gaatataaca cccatggcta gcgtccgatt ctttagggca gagctgtgtc cggtcgtaca
     5821 agaaacagcg gagacgcgtg acacagcaat gtctcttcag gcaccaccga gtaccgccac
     5881 ggaaccgaat catccgccga tctccttcgg agcatcaagc gagacgttcc ccattacatt
     5941 tggggacttc aacgaaggag aaatcgaaag cttgtcttct gagctactaa ctttcggaga
     6001 cttcttacca ggagaagtgg atgacttgac agacagcgac tggtccacgt gctcagacac
     6061 ggacgacgag ttatgactag acagggcagg tgggtatata ttctcgtcgg acaccggtcc
     6121 aggtcattta caacagaagt cagtacgcca gtcagtgctg ccggtgaaca ccctggagga
     6181 agtccacgag gagaagtgtt acccacctaa gctggatgaa gcaaaggagc aactattact
     6241 taagaaactc caggagagtg catccatggc caacagaagc aggtatcagt cgcgcaaagt
     6301 agaaaacatg aaagcagcaa tcatccagag actaaagaga ggctgtagac tatacttaat
     6361 gtcagagacc ccaaaagtcc ctacttaccg gactacatat ccggcgcctg tgtactcgcc
     6421 tccgatcaac gtccgattgt ccaatcccga gtccgcagtg gcagcatgca atgagttctt
     6481 agctagaaac tatccaactg tctcatcata ccaaattacc gacgagtatg atgcatatct
     6541 agacatggtg gacgggtcgg agagttgcct ggaccgagcg acattcaatc cgtcaaaact
     6601 caggagctac ccgaaacagc acgcttacca cgcgccctcc atcagaagcg ctgtaccgtc
     6661 cccattccag aacacactac agaatgtact ggcagcagcc acgaaaagaa actgcaacgt
     6721 cacacagatg agggaattac ccactttgga ctcagcagta ttcaacgtgg agtgtttcaa
     6781 aaaattcgca tgcaaccaag aatactggga agaatttgct gccagcccta ttaggataac
     6841 aactgagaat ttagcaacct atgttactaa actaaaaggg ccaaaagcag cagcgctatt
     6901 cgcaaaaacc cataatctac tgccactaca ggaagtacca atggataggt tcacagtaga
     6961 tatgaaaagg gacgtgaagg tgactcctgg tacaaagcat acagaggaaa gacctaaggt
     7021 gcaggttata caggcggctg aacccttggc gacagcatac ctatgtggga ttcacagaga
     7081 gctggttagg aggctgaacg ccgtcctcct acccaatgta catacactat ttgacatgtc
     7141 tgccgaggat ttcgatgcca tcatagccgc acactttaag ccaggagaca ctgttttgga
     7201 aacggacata gcctcctttg ataagagcca agatgattca cttgcgctta ctgctttgat
     7261 gctgttagag gatttagggg tggatcactc cctgctggac ttgatagagg ctgctttcgg
     7321 agagatttcc agctgtcacc taccgacagg tacgcgcttc aagttcggcg ccatgatgaa
     7381 atcaggtatg ttcctaactc tgttcgtcaa cacattgtta aacatcacca tcgccagccg
     7441 agtgctggaa gatcgtctga caaaatccgc gtgcgcggcc ttcatcggcg acgacaacat
     7501 aatacatgga gtcgtctccg atgaattgat ggcagccaga tgtgccactt ggatgaacat
     7561 ggaagtgaag atcatagatg cagttgtatc cttgaaagcc ccttactttt gtggagggtt
     7621 tatactgcac gatactgtga caggaacagc ttgcagagtg gcagacccgc taaaaaggct
     7681 ttttaaactg ggcaaaccgc tagcggcagg tgacgaacaa gatgaagata gaagacgagc
     7741 gctggctgac gaagtgatca gatggcaacg aacagggcta attgatgagc tggagaaagc
     7801 ggtatactct aggtacgaag tgcagggtat atcagttgtg gtaatgtcca tggccacctt
     7861 tgcgagctcc agatccaact tcgagaagct cagaggaccc gtcataactt tgtacggcgg
     7921 tcctaaatag gtacgcacta cagctaccta ttttgcagaa gccgacagca agtatctaaa
     7981 cactaatcag ctacaatgga gttcatccca acccaaactt tttacaatag gaggtaccag
     8041 cctcgaccct ggactccgcg ctctactatc caagtcatca ggcccagacc gcgccctcag
     8101 aggcaagctg ggcaacttgc ccagctgatc tcagcagtta ataaactgac aatgcgcgcg
     8161 gtaccccaac agaagccacg caggaatcgg aagaataaga agcaaaagca aaaacaacag
     8221 gcgccacaaa acaacacaaa tcaaaagaag cagccaccta aaaagaaacc ggctcaaaag
     8281 aaaaagaagc cgggccgcag agagaggatg tgcatgaaaa tcgaaaatga ttgtattttc
     8341 gaagtcaagc acgaaggtaa ggtaacaggt tacgcgtgcc tggtggggga caaagtaatg
     8401 aaaccagcac acgtaaaggg gaccatcgat aacgcggacc tggccaaact ggcctttaag
     8461 cggtcatcta agtatgacct tgaatgcgcg cagatacccg tgcacatgaa gtccgacgct
     8521 tcgaagttca cccatgagaa accggagggg tactacaact ggcaccacgg agcagtacag
     8581 tactcaggag gccggttcac catccctaca ggtgctggca aaccagggga cagcggcaga
     8641 ccgatcttcg acaacaaggg acgcgtggtg gccatagtct taggaggagc taatgaagga
     8701 gcccgtacag ccctctcggt ggtgacctgg aataaagaca ttgtcactaa aatcaccccc
     8761 gagggggccg aagagtggag tcttgccatc ccagttatgt gcctgttggc aaacaccacg
     8821 ttcccctgct cccagccccc ttgcacgccc tgctgctacg aaaaggaacc ggaggaaacc
     8881 ctacgcatgc ttgaggacaa cgtcatgaga cctgggtact atcagctgct acaagcatcc
     8941 ttaacatgtt ctccccaccg ccagcgacgc agcaccaagg acaacttcaa tgtctataaa
     9001 gccacaagac catacttagc tcactgtccc gactgtggag aagggcactc gtgccatagt
     9061 cccgtagcac tagaacgcat cagaaatgaa gcgacagacg ggacgctgaa aatccaggtc
     9121 tccttgcaaa tcggaataaa gacggatgac agccacgatt ggaccaagct gcgttatatg
     9181 gacaaccaca tgccagcaga cgcagagagg gcggggctat ttgtaagaac atcagcaccg
     9241 tgtacgatta ctggaacaat gggacacttc atcctggccc gatgtccaaa aggggaaact
     9301 ctgacggtgg gattcactga cagtaggaag attagtcatt catgtacgca cccatttcac
     9361 cacgaccctc ctgtgatagg tcgggaaaaa ttccattccc gaccgcagca cggtaaagag
     9421 ctaccttgca gcacgtacgt gcagagcacc gccgcaacta ccgaggagat agaggtacac
     9481 atgcccccag acacccctga tcgcacatta atgtcacaac agtccggcaa cgtaaagatc
     9541 acagtcaatg gccagacggt gcggtacaag tgtaattgcg gtggctcaaa tgaaggacta
     9601 acaactacag acaaagtgat taataactgc aaggttgatc aatgtcatgc cgcggtcacc
     9661 aatcacaaaa agtggcagta taactcccct ctggtcccgc gtaatgctga acttggggac
     9721 cgaaaaggaa aaattcacat cccgtttccg ctggcaaatg taacatgcag ggtgcctaaa
     9781 gcaaggaacc ccaccgtgac gtacgggaaa aaccaagtca tcatgctact gtatcctgac
     9841 cacccaacac tcctgtccta ccggaatatg ggagaagaac caaactatca agaagagtgg
     9901 gtgatgcata agaaggaagt cgtgctaacc gtgccgactg aagggctcga ggtcacgtgg
     9961 ggcaacaacg agccgtataa gtattggccg cagttatcta caaacggtac agcccatggc
    10021 cacccgcatg agataattct gtattattat gagctgtacc ctactatgac tgtagtagtt
    10081 gtgtcagtgg ccacgttcat actcctgtcg atggtgggta tggcagcggg gatgtgcatg
    10141 tgtgcacgac gcagatgcat cacaccgtat gaactgacac caggagctac cgtccctttc
    10201 ctgcttagcc taatatgctg catcagaaca gctaaagcgg ccacatacca agaggctgcg
    10261 atatacctgt ggaacgagca gcaacctttg ttttggctac aagcccttat tccgctggca
    10321 gccctgattg ttctatgcaa ctgtctgaga ctcttaccat gctgctgtaa aacgttggct
    10381 tttttagccg taatgagcgt cggtgcccac actgtgagcg cgtacgaaca cgtaacagtg
    10441 atcccgaaca cggtgggagt accgtataag actctagtca atagacctgg ctacagcccc
    10501 atggtattgg agatggaact actgtcagtc actttggagc caacactatc gcttgattac
    10561 atcacgtgcg agtacaaaac cgtcatcccg tctccgtacg tgaagtgctg cggtacagca
    10621 gagtgcaagg acaaaaacct acctgactac agctgtaagg tcttcaccgg cgtctaccca
    10681 tttatgtggg gcggcgccta ctgcttctgc gacgctgaaa acacgcagtt gagcgaagca
    10741 catgtggaga agtccgaatc atgcaaaaca gaatttgcat cagcatacag ggctcatacc
    10801 gcatctgcat cagctaagct ccgcgtcctt taccaaggaa ataacatcac tgtaactgcc
    10861 tatgcaaacg gcgaccatgc cgtcacagtt aaggacgcca aattcattgt ggggccaatg
    10921 tcttcagcct ggacaccttt cgacaacaaa attgtggtgt acaaaggtga cgtctataac
    10981 atggactacc cgccctttgg cgcaggaaga ccaggacaat ttggcgatat ccaaagtcgc
    11041 acacctgaga gtaaagacgt ctatgctaat acacaactgg tactgcagag accggctgtg
    11101 ggtacggtac acgtgccata ctctcaggca ccatctggct ttaagtattg gctaaaagaa
    11161 cgcggggcgt cactgcagca cacagcacca tttggctgcc aaatagcaac aaacccggta
    11221 agagcggtga actgcgccgt agggaacatg cccatctcca tcgacatacc ggaagcggcc
    11281 ttcactaggg tcgtcgacgc gccctcttta acggacatgt cgtgcgaggt accagcctgc
    11341 acccattcct cagactttgg gggcgtcgcc attattaaat atgcagccag caagaaaggc
    11401 aagtgtgcgg tgcattcgat gactaacgcc gtcactattc gggaagctga gatagaagtt
    11461 gaagggaatt ctcagctgca aatctctttc tcgacggcct tagccagcgc cgaattccgc
    11521 gtacaagtct gttctacaca agtacactgt gcagctgagt gccacccccc gaaggaccac
    11581 atagtcaact acccggcgtc acataccacc ctcggggtcc aggacatctc cgctacggcg
    11641 atgtcatggg tgcagaagat cacgggaggt gtgggactgg ttgttgctgt tgccgcactg
    11701 attctaatcg tggtgctatg cgtgtcgttc agcaggcact aacttgacaa ttaagtatga
    11761 aggtatatgt gtcccctaag agacacactg tacatagcaa ataatctata gatcaaaggg
    11821 ctacgcaacc cctgaatagt aacaaaatac aaaatcacta aaaattataa aaacagaaaa
    11881 atacataaat aggtatacgt gtcccctaag agacacattg tatgtaggtg ataagtatag
    11941 atcaaagggc cgaataaccc ctgaatagta acaaaatatg aaaatcaata aaaatcataa
    12001 aatagaaaaa ccataaacag aagtagttca aagggctata aaacccctga atagtaacaa
    12061 aacataaaat taataaaaat caaatgaata ccataattgg caaacggaag agatgtaggt
    12121 acttaagctt cctaaaagca gccgaactca ctttgagaag taggcatagc ataccgaact
    12181 cttccacgat tctccgaacc cacagggacg taggagatgt tattttgttt ttaatatttc
    12241 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ctagtgcggc cgcagcttgg
    12301 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
    12361 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
    12421 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
    12481 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
    12541 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    12601 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    12661 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    12721 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    12781 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    12841 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    12901 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    12961 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    13021 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    13081 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    13141 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    13201 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    13261 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    13321 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    13381 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    13441 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    13501 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    13561 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    13621 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    13681 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    13741 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    13801 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    13861 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    13921 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    13981 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    14041 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    14101 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    14161 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    14221 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    14281 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    14341 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    14401 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    14461 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
    14521 ggccctttcg tc
//
