LOCUS       V008520                14715 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008520
VERSION     V008520
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14715)
  AUTHORS   Scholte FE, Tas A, Martina BE, Cordioli P, Narayanan K, Makino S,
            Snijder EJ, van Hemert MJ.
  TITLE     Characterization of Synthetic Chikungunya Viruses Based on the
            Consensus Sequence of Recent E1-226V Isolates
  JOURNAL   PLoS ONE 8 (8), E71047 (2013)
   PUBMED   23936484
REFERENCE   2  (bases 1 to 14715)
  AUTHORS   Scholte FEM., Tas A, Snijder EJ, van Hemert MJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2012) Medical Microbiology, Leiden University
            Medical Center, Albinusdreef 2, Leiden, Zuid-Holland 2333 ZA, The
            Netherlands
REFERENCE   3  (bases 1 to 14715)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14715)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2013"; volume: "8"; issue: "8"; pages: "E71047"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (07-OCT-2012) Medical Microbiology, Leiden University Medical
            Center, Albinusdreef 2, Leiden, Zuid-Holland 2333 ZA, The
            Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      410..430
                     /regulatory_class="promoter"
     5'UTR           430..505
     CDS             506..7930
                     /codon_start=1
                     /transl_except=(pos: 6074 .. 6076,  aa: OTHER)
                     /product="nonstructural polyprotein"
                     /label="nonstructural polyprotein"
                     /note="encodes nonstructural proteins 1 through 4"
                     /protein_id="AGN96203.1"
                     /translation="MDPVYVDIDADSAFLKALQRAYPMFEVEPRQVTPNDHANARAFSH
                     LAIKLIEQEIDPDSTILDIGSAPARRMMSDRKYHCVCPMRSAEDPERLANYARKLASAA
                     GKVLDRNISGKIGDLQAVMAVPDKETPTFCLHTDVSCRQRADVAIYQDVYAVHAPTSLY
                     HQAIKGVRVAYWVGFDTTPFMYNAMAGAYPSYSTNWADEQVLKAKNIGLCSTDLTEGRR
                     GKLSIMRGKKLKPCDRVLFSVGSTLYPESRKLLKSWHLPSVFHLKGKLSFTCRCDTVVS
                     CEGYVVKRITMSPGLYGKTTGYAVTHHADGFLMCKTTDTVDGERVSFSVCTYVPATICD
                     QMTGILATEVTPEDAQKLLVGLNQRIVVNGRTQRNMNTMKNYLLPVVAQAFSKWAKECR
                     KDMEDEKLLGVRERTLTCCCLWAFKKQKTHTVYKRPDTQSIQKVQAEFDSFVVPSLWSS
                     GLSIPLRTRIKWLLSKVPKTDLIPYSGDAREARDAEKEAEEEREAELTREALPPLQAAQ
                     EDVQVEIDVEQLEDRAGAGIIETPRGAIKVTAQPTDHVVGEYLVLSPQTVLRSQKLSLI
                     HALAEQVKTCTHNGRAGRYAVEAYDGRVLVPSGYAISPEDFQSLSESATMVYNEREFVN
                     RKLHHIAMHGPALNTDEESYELVRAERTEHEYVYDVDQRRCCKKEEAAGLVLVGDLTNP
                     PYHEFAYEGLKIRPACPYKIAVIGVFGVPGSGKSAIIKNLVTRQDLVTSGKKENCQEIT
                     TDVMRQRGLEISARTVDSLLLNGCNRPVDVLYVDEAFACHSGTLLALIALVRPRQKVVL
                     CGDPKQCGFFNMMQMKVNYNHNICTQVYHKSISRRCTLPVTAIVSSLHYEGKMRTTNEY
                     NKPIVVDTTGSTKPDPGDLVLTCFRGWVKQLQIDYRGYEVMTAAASQGLTRKGVYAVRQ
                     KVNENPLYASTSEHVNVLLTRTEGKLVWKTLSGDPWIKTLQNPPKGNFKATIKEWEVEH
                     ASIMAGICSHQMTFDTFQNKANVCWAKSLVPILETAGIKLNDRQWSQIIQAFKEDKAYS
                     PEVALNEICTRMYGVDLDSGLFSKPLVSVYYADNHWDNRPGGKMFGFNPEAASILERKY
                     PFTKGKWNINKQICVTTRRIEDFNPTTNIIPANRRLPHSLVAEHRPVKGERMEWLVNKI
                     NGHHVLLVSGYNLALPTKRVTWVAPLGVRGADYTYNLELGLPATLGRYDLVVINIHTPF
                     RIHHYQQCVDHAMKLQMLGGDSLRLLKPGGSLLIRAYGYADRTSERVICVLGRKFRSSR
                     ALKPPCVTSNTEMFFLFSNFDNGRRNFTTHVMNNQLNAAFVGQVTRAGCAPSYRVKRMD
                     IAKNDEECVVNAANPRGLPGDGVCKAVYKKWPESFKNSATPVGTAKTVMCGTYPVIHAV
                     GPNFSNYSESEGDRELAAAYREVAKEVTRLGVNSVAIPLLSTGVYSGGKDRLTQSLNHL
                     FTAMDSTDADVVIYCRDKEWEKKISEAIQMRTQVELLDEHISIDCDIVRVHPDSSLAGR
                     KGYSTTEGALYSYLEGTRFHQTAVDMAEIHTMWPKQTEANEQVCLYALGESIESIRQKC
                     PVDDADASSPPKTVPCLCRYAMTPERVTRLRMNHVTSIIVCSSFPLPKYKIEGVQKVKC
                     SKVMLFDHNVPSRVSPREYRSSQESAQEASTITSLTHSQFDLSVDGEILPVPSDLDADA
                     PALEPALDDGATHTLPSTTGNLAAVSDWVMSTVPVAPPRRRRGRNLTVTCDEREGNITP
                     MASVRFFRAELCPVVQETAETRDTAMSLQAPPSTATEPNHPPISFGASSETFPITFGDF
                     NEGEIESLSSELLTFGDFLPGEVDDLTDSDWSTCSDTDDEL*LDRAGGYIFSSDTGPGH
                     LQQKSVRQSVLPVNTLEEVHEEKCYPPKLDEAKEQLLLKKLQESASMANRSRYQSRKVE
                     NMKAAIIQRLKRGCRLYLMSETPKVPTYRTTYPAPVYSPPINVRLSNPESAVAACNEFL
                     ARNYPTVSSYQITDEYDAYLDMVDGSESCLDRATFNPSKLRSYPKQHAYHAPSIRSAVP
                     SPFQNTLQNVLAAATKRNCNVTQMRELPTLDSAVFNVECFKKFACNQEYWEEFAASPIR
                     ITTENLATYVTKLKGPKAAALFAKTHNLLPLQEVPMDRFTVDMKRDVKVTPGTKHTEER
                     PKVQVIQAAEPLATAYLCGIHRELVRRLNAVLLPNVHTLFDMSAEDFDAIIAAHFKPGD
                     TVLETDIASFDKSQDDSLALTALMLLEDLGVDHSLLDLIEAAFGEISSCHLPTGTRFKF
                     GAMMKSGMFLTLFVNTLLNITIASRVLEDRLTKSACAAFIGDDNIIHGVVSDELMAARC
                     ATWMNMEVKIIDAVVSLKAPYFCGGFILHDTVTGTACRVADPLKRLFKLGKPLAAGDEQ
                     DEDRRRALADEVIRWQRTGLIDELEKAVYSRYEVQGISVVVMSMATFASSRSNFEKLRG
                     PVITLYGGPK"
     regulatory      7909..7928
                     /regulatory_class="promoter"
     regulatory      8092..8111
                     /regulatory_class="promoter"
     CDS             8179..11922
                     /note="Structural polyprotein from Chikungunya virus
                     (strain S27-African prototype). Accession#: Q8JUX5"
                     /label="Structural polyprotein"
     3'UTR           11926..12463
     primer_bind     complement(12494..12510)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    12518..12534
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12542..12572)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(12587..12608)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(12896..13484)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(13658..14515)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(14516..14620)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ggcgcgccac agtaatacga
      421 ctcactatta tggctgcgtg agacacacgt agcctaccag tttcttactg ctctactctg
      481 caaagcaaga gattaataac ccatcatgga tcctgtgtac gtggacatag acgctgacag
      541 cgcctttttg aaggccctgc aacgtgcgta ccccatgttt gaggtggaac caaggcaggt
      601 cacaccgaat gaccatgcta atgctagagc gttctcgcat ctagctataa aactaataga
      661 gcaggaaatt gaccccgact caaccatcct ggatatcggc agtgcgccag caaggaggat
      721 gatgtcggac aggaagtacc actgcgtctg cccgatgcgc agtgcggaag atcccgagag
      781 actcgctaat tatgcgagaa agctagcatc tgccgcagga aaagtcctgg acagaaacat
      841 ctctggaaag atcggggact tacaagcagt aatggccgtg ccagacaagg agacgccaac
      901 attctgctta cacacagacg tctcatgtag acagagagca gacgtcgcta tataccaaga
      961 cgtctatgct gtacacgcac ccacgtcgct ataccaccag gcgattaaag gggtccgagt
     1021 ggcgtactgg gttgggttcg acacaacccc gttcatgtac aatgccatgg cgggtgccta
     1081 cccctcatac tcgacaaact gggcagatga gcaggtactg aaggctaaga acataggatt
     1141 atgttcaaca gacctgacgg aaggtagacg aggcaagttg tctattatga gagggaaaaa
     1201 gctaaaaccg tgcgaccgtg tgctgttctc agtagggtca acgctctacc cggaaagccg
     1261 caagctactt aagagctggc acctgccatc ggtgttccat ttaaagggca aactcagctt
     1321 cacatgccgc tgtgatacag tggtttcgtg tgagggctac gtcgttaaga gaataacgat
     1381 gagcccaggc ctttatggaa aaaccacagg gtatgcggta acccaccacg cagacggatt
     1441 cctgatgtgc aagactaccg acacggttga cggcgaaaga gtgtcattct cggtgtgcac
     1501 atacgtgccg gcgaccattt gtgatcaaat gaccggcatc cttgctacag aagtcacgcc
     1561 ggaggatgca cagaagctgt tggtggggct gaaccagaga atagtggtta acggcagaac
     1621 gcaacggaat atgaacacca tgaaaaatta tctgcttccc gtggtcgccc aagccttcag
     1681 taagtgggca aaggagtgcc ggaaagacat ggaagatgaa aaactcctgg gggtcagaga
     1741 aagaacactg acctgctgct gtctatgggc attcaagaag cagaaaacac acacggtcta
     1801 caagaggcct gatacccagt caattcagaa ggttcaggcc gagtttgaca gctttgtggt
     1861 accgagtctg tggtcgtccg ggttgtcaat ccctttgagg actagaatca aatggttgtt
     1921 aagcaaggtg ccaaaaaccg acctgatccc atacagcgga gacgcccgag aagcccggga
     1981 cgcagaaaaa gaagcagagg aagaacgaga agcagaactg actcgcgaag ccctaccacc
     2041 tctacaggca gcacaggaag atgttcaggt cgaaatcgac gtggaacagc ttgaggacag
     2101 agcgggcgca ggaataatag agactccgag aggagctatc aaagttactg cccaaccaac
     2161 agaccacgtc gtgggagagt acctggtact ctccccgcag accgtactac gtagccagaa
     2221 gctcagtctg attcacgctt tggcggagca agtgaagacg tgcacgcaca acggacgagc
     2281 agggaggtat gcggtcgaag cgtacgacgg ccgagtccta gtgccctcag gctatgcaat
     2341 ctcgcctgaa gacttccaga gtctaagcga aagcgcaacg atggtgtata acgaaagaga
     2401 gttcgtaaac agaaagctac accatattgc gatgcacgga ccagccctga acaccgacga
     2461 agagtcgtat gagctggtga gggcagagag gacagaacac gagtacgtct acgacgtgga
     2521 tcagagaaga tgctgtaaga aggaagaagc cgcaggactg gtactggtgg gcgacttgac
     2581 taatccgccc taccacgaat tcgcatatga agggctaaaa atccgccctg cctgcccata
     2641 caaaattgca gtcataggag tcttcggagt accgggatct ggcaagtcag ctattatcaa
     2701 gaacctagtt accaggcagg acctggtgac tagcggaaag aaagaaaact gccaagaaat
     2761 caccaccgac gtgatgagac agagaggtct agagatatct gcacgtacgg ttgactcgct
     2821 gctcttgaat ggatgcaaca gaccagtcga cgtgttgtac gtagacgagg cgtttgcgtg
     2881 ccactctgga acgctacttg ctttgatcgc cttggtgaga ccaaggcaga aagttgtact
     2941 ttgtggtgac ccgaagcagt gcggcttctt caatatgatg cagatgaaag tcaactataa
     3001 tcacaacatc tgcacccaag tgtaccacaa aagtatctcc aggcggtgta cactgcctgt
     3061 gaccgccatt gtgtcatcgt tgcattacga aggcaaaatg cgcactacga atgagtacaa
     3121 caagccgatt gtagtggaca ctacaggctc aacaaaacct gaccctggag acctcgtgtt
     3181 aacgtgcttc agagggtggg ttaaacaact gcaaattgac tatcgtggat acgaggtcat
     3241 gacagcagcc gcatcccaag ggttaaccag aaaaggagtt tacgcagtta gacaaaaagt
     3301 taatgaaaac ccgctctatg catcaacgtc agagcacgtc aacgtactcc taacgcgtac
     3361 ggaaggtaaa ctggtatgga agacactttc cggcgacccg tggataaaga cgctgcagaa
     3421 cccaccgaaa ggaaacttca aagcaactat taaggagtgg gaggtggagc atgcatcaat
     3481 aatggcgggc atctgcagtc accaaatgac cttcgataca ttccaaaata aagccaacgt
     3541 ttgttgggct aagagcttgg tccctatcct cgaaacagcg gggataaaac taaatgatag
     3601 gcagtggtct cagataattc aagccttcaa agaagacaaa gcatactcac ctgaagtagc
     3661 cctgaatgaa atatgtacgc gcatgtatgg ggtggatcta gacagcgggc tattttctaa
     3721 accgttggtg tctgtgtatt acgcggataa ccactgggat aataggcctg gagggaaaat
     3781 gttcggattt aaccccgagg cagcatccat tctagaaaga aagtatccat tcacaaaagg
     3841 gaagtggaac atcaacaagc agatctgcgt gactaccagg aggatagaag actttaaccc
     3901 taccaccaac atcataccgg ccaacaggag actaccacac tcattagtgg ccgaacaccg
     3961 cccagtaaaa ggggaaagaa tggaatggct ggttaacaag ataaacggcc accacgtgct
     4021 cctggtcagt ggctataacc ttgcactgcc tactaagaga gtcacttggg tagcgccgtt
     4081 aggtgtccgc ggagcggact acacatacaa cctagagttg ggtctgccag caacgcttgg
     4141 taggtatgac cttgtggtca taaacatcca cacacctttt cgcatacacc attaccaaca
     4201 gtgcgtcgac cacgcaatga aactgcaaat gctcgggggt gactcattga gactgctcaa
     4261 accgggcggc tctctattga tcagagcata tggttacgca gatagaacca gtgaacgagt
     4321 catctgcgta ttgggacgca agtttagatc gtctagagcg ttgaaaccac catgtgtcac
     4381 cagcaacact gagatgtttt tcctattcag caactttgac aatggcagaa ggaatttcac
     4441 aactcatgtc atgaacaatc aactgaatgc agccttcgta ggacaggtca cccgagcagg
     4501 atgtgcaccg tcgtaccggg taaaacgcat ggacatcgcg aagaacgatg aagagtgcgt
     4561 agtcaacgcc gctaaccctc gcgggttacc gggtgacggt gtttgcaagg cagtatacaa
     4621 aaaatggccg gagtccttta agaacagtgc aacaccagtg ggaaccgcaa aaacagttat
     4681 gtgcggtacg tatccagtaa tccacgctgt tggaccaaac ttctctaatt attcggagtc
     4741 tgaaggggac cgggaattgg cagctgccta tcgagaagtc gcaaaggaag taactaggct
     4801 gggagtaaat agtgtagcta tacctctcct ctccacaggt gtatactcag gagggaaaga
     4861 caggctgacc cagtcactga accacctctt tacagccatg gactcgacgg atgcagacgt
     4921 ggtcatctac tgccgcgaca aagaatggga gaagaaaata tctgaggcca tacagatgcg
     4981 gacccaagta gagctgctgg atgagcacat ctccatagac tgcgatattg ttcgcgtgca
     5041 ccctgacagc agcttggcag gcagaaaagg atacagcacc acggaaggcg cactgtactc
     5101 atatctagaa gggacccgtt ttcatcagac ggctgtggat atggcggaga tacatactat
     5161 gtggccaaag caaacagagg ccaatgagca agtctgccta tatgccctgg gggaaagtat
     5221 tgaatcgatc aggcagaaat gcccggtgga tgatgcagac gcatcatctc cccccaaaac
     5281 tgtcccgtgc ctttgccgtt acgctatgac tccagaacgc gtcacccggc ttcgcatgaa
     5341 ccacgtcaca agcataattg tgtgttcttc gtttcccctc ccaaagtaca aaatagaagg
     5401 agtgcaaaaa gtcaaatgct ctaaggtaat gctatttgac cacaacgtgc catcgcgcgt
     5461 aagtccaagg gaatatagat cttcccagga gtctgcacag gaggcgagta caatcacgtc
     5521 actgacgcat agtcaattcg acctaagcgt tgatggcgag atactgcccg tcccgtcaga
     5581 cctggatgct gacgccccag ccctagaacc agcactagac gacggggcga cacacacgct
     5641 gccatccaca accggaaacc ttgcggccgt gtctgactgg gtaatgagca ccgtacctgt
     5701 cgcgccgccc agaagaaggc gagggagaaa cctgactgtg acatgtgacg agagagaagg
     5761 gaatataaca cccatggcta gcgtccgatt ctttagggca gagctgtgtc cggtcgtaca
     5821 agaaacagcg gagacgcgtg acacagcaat gtctcttcag gcaccaccga gtaccgccac
     5881 ggaaccgaat catccgccga tctccttcgg agcatcaagc gagacgttcc ccattacatt
     5941 tggggacttc aacgaaggag aaatcgaaag cttgtcttct gagctactaa ctttcggaga
     6001 cttcttacca ggagaagtgg atgacttgac agacagcgac tggtccacgt gctcagacac
     6061 ggacgacgag ttatgactag acagggcagg tgggtatata ttctcgtcgg acaccggtcc
     6121 aggtcattta caacagaagt cagtacgcca gtcagtgctg ccggtgaaca ccctggagga
     6181 agtccacgag gagaagtgtt acccacctaa gctggatgaa gcaaaggagc aactattact
     6241 taagaaactc caggagagtg catccatggc caacagaagc aggtatcagt cgcgcaaagt
     6301 agaaaacatg aaagcagcaa tcatccagag actaaagaga ggctgtagac tatacttaat
     6361 gtcagagacc ccaaaagtcc ctacttaccg gactacatat ccggcgcctg tgtactcgcc
     6421 tccgatcaac gtccgattgt ccaatcccga gtccgcagtg gcagcatgca atgagttctt
     6481 agctagaaac tatccaactg tctcatcata ccaaattacc gacgagtatg atgcatatct
     6541 agacatggtg gacgggtcgg agagttgcct ggaccgagcg acattcaatc cgtcaaaact
     6601 caggagctac ccgaaacagc acgcttacca cgcgccctcc atcagaagcg ctgtaccgtc
     6661 cccattccag aacacactac agaatgtact ggcagcagcc acgaaaagaa actgcaacgt
     6721 cacacagatg agggaattac ccactttgga ctcagcagta ttcaacgtgg agtgtttcaa
     6781 aaaattcgca tgcaaccaag aatactggga agaatttgct gccagcccta ttaggataac
     6841 aactgagaat ttagcaacct atgttactaa actaaaaggg ccaaaagcag cagcgctatt
     6901 cgcaaaaacc cataatctac tgccactaca ggaagtacca atggataggt tcacagtaga
     6961 tatgaaaagg gacgtgaagg tgactcctgg tacaaagcat acagaggaaa gacctaaggt
     7021 gcaggttata caggcggctg aacccttggc gacagcatac ctatgtggga ttcacagaga
     7081 gctggttagg aggctgaacg ccgtcctcct acccaatgta catacactat ttgacatgtc
     7141 tgccgaggat ttcgatgcca tcatagccgc acactttaag ccaggagaca ctgttttgga
     7201 aacggacata gcctcctttg ataagagcca agatgattca cttgcgctta ctgctttgat
     7261 gctgttagag gatttagggg tggatcactc cctgctggac ttgatagagg ctgctttcgg
     7321 agagatttcc agctgtcacc taccgacagg tacgcgcttc aagttcggcg ccatgatgaa
     7381 atcaggtatg ttcctaactc tgttcgtcaa cacattgtta aacatcacca tcgccagccg
     7441 agtgctggaa gatcgtctga caaaatccgc gtgcgcggcc ttcatcggcg acgacaacat
     7501 aatacatgga gtcgtctccg atgaattgat ggcagccaga tgtgccactt ggatgaacat
     7561 ggaagtgaag atcatagatg cagttgtatc cttgaaagcc ccttactttt gtggagggtt
     7621 tatactgcac gatactgtga caggaacagc ttgcagagtg gcagacccgc taaaaaggct
     7681 ttttaaactg ggcaaaccgc tagcggcagg tgacgaacaa gatgaagata gaagacgagc
     7741 gctggctgac gaagtgatca gatggcaacg aacagggcta attgatgagc tggagaaagc
     7801 ggtatactct aggtacgaag tgcagggtat atcagttgtg gtaatgtcca tggccacctt
     7861 tgcgagctcc agatccaact tcgagaagct cagaggaccc gtcataactt tgtacggcgg
     7921 tcctaaatag gtacgcacta cagctaccta ttttgcagaa gccgacagca agtatctaaa
     7981 cactaatcag ctacagcgat taagggccca attgcctagg gtttaaactc cggacctgca
     8041 gggcccgagc tccagatcca acttcgagaa gctcagagga cccgtcataa ctttgtacgg
     8101 cggtcctaaa taggtacgca ctacagctac ctattttgca gaagccgaca gcaagtatct
     8161 aaacactaat cagctacaat ggagttcatc ccaacccaaa ctttttacaa taggaggtac
     8221 cagcctcgac cctggactcc gcgctctact atccaagtca tcaggcccag accgcgccct
     8281 cagaggcaag ctgggcaact tgcccagctg atctcagcag ttaataaact gacaatgcgc
     8341 gcggtacccc aacagaagcc acgcaggaat cggaagaata agaagcaaaa gcaaaaacaa
     8401 caggcgccac aaaacaacac aaatcaaaag aagcagccac ctaaaaagaa accggctcaa
     8461 aagaaaaaga agccgggccg cagagagagg atgtgcatga aaatcgaaaa tgattgtatt
     8521 ttcgaagtca agcacgaagg taaggtaaca ggttacgcgt gcctggtggg ggacaaagta
     8581 atgaaaccag cacacgtaaa ggggaccatc gataacgcgg acctggccaa actggccttt
     8641 aagcggtcat ctaagtatga ccttgaatgc gcgcagatac ccgtgcacat gaagtccgac
     8701 gcttcgaagt tcacccatga gaaaccggag gggtactaca actggcacca cggagcagta
     8761 cagtactcag gaggccggtt caccatccct acaggtgctg gcaaaccagg ggacagcggc
     8821 agaccgatct tcgacaacaa gggacgcgtg gtggccatag tcttaggagg agctaatgaa
     8881 ggagcccgta cagccctctc ggtggtgacc tggaataaag acattgtcac taaaatcacc
     8941 cccgaggggg ccgaagagtg gagtcttgcc atcccagtta tgtgcctgtt ggcaaacacc
     9001 acgttcccct gctcccagcc cccttgcacg ccctgctgct acgaaaagga accggaggaa
     9061 accctacgca tgcttgagga caacgtcatg agacctgggt actatcagct gctacaagca
     9121 tccttaacat gttctcccca ccgccagcga cgcagcacca aggacaactt caatgtctat
     9181 aaagccacaa gaccatactt agctcactgt cccgactgtg gagaagggca ctcgtgccat
     9241 agtcccgtag cactagaacg catcagaaat gaagcgacag acgggacgct gaaaatccag
     9301 gtctccttgc aaatcggaat aaagacggat gacagccacg attggaccaa gctgcgttat
     9361 atggacaacc acatgccagc agacgcagag agggcggggc tatttgtaag aacatcagca
     9421 ccgtgtacga ttactggaac aatgggacac ttcatcctgg cccgatgtcc aaaaggggaa
     9481 actctgacgg tgggattcac tgacagtagg aagattagtc attcatgtac gcacccattt
     9541 caccacgacc ctcctgtgat aggtcgggaa aaattccatt cccgaccgca gcacggtaaa
     9601 gagctacctt gcagcacgta cgtgcagagc accgccgcaa ctaccgagga gatagaggta
     9661 cacatgcccc cagacacccc tgatcgcaca ttaatgtcac aacagtccgg caacgtaaag
     9721 atcacagtca atggccagac ggtgcggtac aagtgtaatt gcggtggctc aaatgaagga
     9781 ctaacaacta cagacaaagt gattaataac tgcaaggttg atcaatgtca tgccgcggtc
     9841 accaatcaca aaaagtggca gtataactcc cctctggtcc cgcgtaatgc tgaacttggg
     9901 gaccgaaaag gaaaaattca catcccgttt ccgctggcaa atgtaacatg cagggtgcct
     9961 aaagcaagga accccaccgt gacgtacggg aaaaaccaag tcatcatgct actgtatcct
    10021 gaccacccaa cactcctgtc ctaccggaat atgggagaag aaccaaacta tcaagaagag
    10081 tgggtgatgc ataagaagga agtcgtgcta accgtgccga ctgaagggct cgaggtcacg
    10141 tggggcaaca acgagccgta taagtattgg ccgcagttat ctacaaacgg tacagcccat
    10201 ggccacccgc atgagataat tctgtattat tatgagctgt accctactat gactgtagta
    10261 gttgtgtcag tggccacgtt catactcctg tcgatggtgg gtatggcagc ggggatgtgc
    10321 atgtgtgcac gacgcagatg catcacaccg tatgaactga caccaggagc taccgtccct
    10381 ttcctgctta gcctaatatg ctgcatcaga acagctaaag cggccacata ccaagaggct
    10441 gcgatatacc tgtggaacga gcagcaacct ttgttttggc tacaagccct tattccgctg
    10501 gcagccctga ttgttctatg caactgtctg agactcttac catgctgctg taaaacgttg
    10561 gcttttttag ccgtaatgag cgtcggtgcc cacactgtga gcgcgtacga acacgtaaca
    10621 gtgatcccga acacggtggg agtaccgtat aagactctag tcaatagacc tggctacagc
    10681 cccatggtat tggagatgga actactgtca gtcactttgg agccaacact atcgcttgat
    10741 tacatcacgt gcgagtacaa aaccgtcatc ccgtctccgt acgtgaagtg ctgcggtaca
    10801 gcagagtgca aggacaaaaa cctacctgac tacagctgta aggtcttcac cggcgtctac
    10861 ccatttatgt ggggcggcgc ctactgcttc tgcgacgctg aaaacacgca gttgagcgaa
    10921 gcacatgtgg agaagtccga atcatgcaaa acagaatttg catcagcata cagggctcat
    10981 accgcatctg catcagctaa gctccgcgtc ctttaccaag gaaataacat cactgtaact
    11041 gcctatgcaa acggcgacca tgccgtcaca gttaaggacg ccaaattcat tgtggggcca
    11101 atgtcttcag cctggacacc tttcgacaac aaaattgtgg tgtacaaagg tgacgtctat
    11161 aacatggact acccgccctt tggcgcagga agaccaggac aatttggcga tatccaaagt
    11221 cgcacacctg agagtaaaga cgtctatgct aatacacaac tggtactgca gagaccggct
    11281 gtgggtacgg tacacgtgcc atactctcag gcaccatctg gctttaagta ttggctaaaa
    11341 gaacgcgggg cgtcactgca gcacacagca ccatttggct gccaaatagc aacaaacccg
    11401 gtaagagcgg tgaactgcgc cgtagggaac atgcccatct ccatcgacat accggaagcg
    11461 gccttcacta gggtcgtcga cgcgccctct ttaacggaca tgtcgtgcga ggtaccagcc
    11521 tgcacccatt cctcagactt tgggggcgtc gccattatta aatatgcagc cagcaagaaa
    11581 ggcaagtgtg cggtgcattc gatgactaac gccgtcacta ttcgggaagc tgagatagaa
    11641 gttgaaggga attctcagct gcaaatctct ttctcgacgg ccttagccag cgccgaattc
    11701 cgcgtacaag tctgttctac acaagtacac tgtgcagctg agtgccaccc cccgaaggac
    11761 cacatagtca actacccggc gtcacatacc accctcgggg tccaggacat ctccgctacg
    11821 gcgatgtcat gggtgcagaa gatcacggga ggtgtgggac tggttgttgc tgttgccgca
    11881 ctgattctaa tcgtggtgct atgcgtgtcg ttcagcaggc actaacttga caattaagta
    11941 tgaaggtata tgtgtcccct aagagacaca ctgtacatag caaataatct atagatcaaa
    12001 gggctacgca acccctgaat agtaacaaaa tacaaaatca ctaaaaatta taaaaacaga
    12061 aaaatacata aataggtata cgtgtcccct aagagacaca ttgtatgtag gtgataagta
    12121 tagatcaaag ggccgaataa cccctgaata gtaacaaaat atgaaaatca ataaaaatca
    12181 taaaatagaa aaaccataaa cagaagtagt tcaaagggct ataaaacccc tgaatagtaa
    12241 caaaacataa aattaataaa aatcaaatga ataccataat tggcaaacgg aagagatgta
    12301 ggtacttaag cttcctaaaa gcagccgaac tcactttgag aagtaggcat agcataccga
    12361 actcttccac gattctccga acccacaggg acgtaggaga tgttattttg tttttaatat
    12421 ttcaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaactagtgc ggccgcagct
    12481 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
    12541 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
    12601 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
    12661 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
    12721 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
    12781 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
    12841 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
    12901 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
    12961 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
    13021 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
    13081 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
    13141 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
    13201 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    13261 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    13321 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    13381 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    13441 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    13501 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    13561 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    13621 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    13681 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    13741 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    13801 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    13861 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    13921 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    13981 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    14041 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    14101 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    14161 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    14221 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    14281 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    14341 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    14401 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    14461 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    14521 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    14581 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    14641 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
    14701 cgaggccctt tcgtc
//
