LOCUS       V008523                13826 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008523
VERSION     V008523
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13826)
  AUTHORS   Tsetsarkin KA, Vanlandingham DL, Higgs S.
  TITLE     Full length infectious clone of Chikungunya virus strain 37997
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13826)
  AUTHORS   Tsetsarkin KA, Vanlandingham DL, Higgs S.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2007) Pathology, University of Texas Medical
            Branch, University Blvd, Galveston, TX 77555-0609, USA
REFERENCE   3  (bases 1 to 13826)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13826)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (17-OCT-2007) Pathology, University of Texas Medical Branch,
            University Blvd, Galveston, TX 77555-0609, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     5'UTR           1..77
     CDS             78..7502
                     /codon_start=1
                     /transl_except=(pos: 5646 .. 5648,  aa: OTHER)
                     /product="nonstructural polyprotein"
                     /label="nonstructural polyprotein"
                     /note="encodes nonstructural proteins 1 through 4"
                     /protein_id="ABX40010.1"
                     /translation="MDPVYVDIDADSAFLKALQRAYPMFEVEPRQVTPNDHANARAFSH
                     LAIKLIEQEIDPDSTILDIGSAPARRMMSDRKYHCVCPMRSAEDPERLANYARKLASAA
                     GKVLDRNISEKIGDLQAVMAVPDAETPTFCLHTDVSCRQRADVAIYQDVYAVHAPTSLY
                     HQAIKGVRVAYWIGFDTTPFMYNAMAGAYPSYSTNWADEQVLKAKNIGLCSTDLTEGRR
                     GKLSIMRGKKMKPCDRVLFSVGSTLYPESRKLLKSWHLPSVFHLKGKLSFTCRCDTVVS
                     CEGYVVKRITISPGLYGKTTGYAVTHHADGFLMCKTTDTVDGERVSFSVCTYVPATICD
                     QMTGILATEVTPEDAQKLLVGLNQRIVVNGRTQRNTNTMKNYLLPVVAQALSKWAKECR
                     KDMEDEKLLGIRERTLTCCCLWAFKKQKTHTVYKRPDTQSIQKVPAEFDSFVVPSLWSS
                     GLSIPLRTRIKWLLSKVPKTDLIPYSGDAKEARDAEKEAEEEREAELTREALPPLQAAQ
                     DDVQVEIDVEQLEDRAGAGIIETPRGAIKVTAQPTDHVVGEYLVLSPQTVLRSQKLSLI
                     HALAEQVKTCTHSGRAGRYAVEAYDGRILVPSGYAISPEDFQSLSESATMVYNEREFVN
                     RKLHHIALHGPALNTDEESYELVRAERTEHEYVYDVDQRRCCKKEEAAGLVLVGDLTNP
                     PYHEFAYEGLRIRPACPYKTAVIGVFGVPGSGKSAIIKNLVTRQDLVTSGKKENCQEIS
                     TDVMRQRNLEISARTVDSLLLNGCNRPVDVLYVDEAFACHSGTLLALIALVRPRQKVVL
                     CGDPKQCGFFNMMQMKVNYNHNICTQVYHKSISRRCTLPVTAIVSSLHYEGKMRTTNEY
                     NKPIVVDTTGSTKPDPGDLVLTCFRGWVKQLQIDYRGHEVMTAAASQGLTRKGVYAVRQ
                     KVNENPLYASTSEHVNVLLTRTEGKLVWKTLSGDPWIKTLQNPPKGNFKATIKEWEVEH
                     ASIMAGICNHQVTFDTFQNKANVCWAKSLVPILETAGIKLNDRQWSQIIQAFKEDRAYS
                     PEVALNEICTRMYGVDLDSGLFSKPLVSVHHADNHWDNRPGGKMFGFNPEAASILERKY
                     PFTKGKWNTNKQICVTTRRIEDFNPNTNIIPANRRLPHSLVAEHRPVKGERMEWLVNKI
                     NGHHVLLVSGYNLVLPTKRVTWVAPLGIRGADYTYNLELGLPATLGRYDLVIINIHTPF
                     RIHHYQQCVDHAMKLQMLGGDSLRLLKPGGSLLIRAYGYADRTSERVVCVLGRKFRSSR
                     ALKPPCVTSNTEMFFLFSNFDNGRRNFTTHVMNNQLNAAFVGQATRAGCAPSYRVKRMD
                     IAKNDEECVVNAANPRGLPGDGVCKAVYKKWPESFKNSATPVGTAKTVMCGTYPVIHAV
                     GPNFSNYSESEGDRELAAAYREVAKEVTRLGVNSVAIPLLSTGVYSGGKDRLTQSLNHL
                     FTALDSTDADVVIYCRDKEWEKKIAEAIQMRTQVELLDEHISVDCDIIRVHPDSSLAGR
                     KGYSTTEGSLYSYLEGTRFHQTAVDMAEVYTMWPKQTEANEQVCLYALGESIESIRQKC
                     PVDDADASSPPKTVPCLCRYAMTPERVTRLRMNHVTSIIVCSSFPLPKYKIEGVQKVKC
                     SKVMLFDHNVPSRVSPREYKSPQETAQEVSSTTSLTHSQFDLSVDGEELPAPSDLEADA
                     PIPEPTPDDRAVLTLPPTIDNFSAVSDWVMNTAPVAPPRRRRGKNLNVTCDEREGNVLP
                     MASVRFFRADLHSIVQETAEIRDTAASLQAPLSVATEPNQLPISFGAPNETFPITFGDF
                     DEGEIESLSSELLTFGDFSPGEVDDLTDSDWSTCSDTDDEL*LDRAGGYIFSSDTGPGH
                     LQQRSVRQTVLPVNTLEEVQEEKCYPPKLDEVKEQLLLKKLQESASMANRSRYQSRKVE
                     NMKATIVQRLKGGCKLYLMSETPKVPTYRTTYPAPVYSPPINIRLSNPESAVAACNEFL
                     ARNYPTVASYQITDEYDAYLDMVDGSESCLDRATFNPSKLRSYPKQHSYHAPTIRSAVP
                     SPFQNTLQNVLAAATKRNCNVTQMRELPTLDSAVFNVECFKKFACNQEYWKEFAASPIR
                     ITTENLTTYVTKLKGPKAAALFAKTHNLLPLQEVPMDRFTVDMKRDVKVTPGTKHTEER
                     PKVQVIQAAEPLATAYLCGIHRELVRRLNAVLLPNVHTLFDMSAEDFDAIIAAHFKPGD
                     AVLETDIASFDKSQDDSLALTALMLLEDLGVDHPLLDLIEAAFGEISSCHLPTGTRFKF
                     GAMMKSGMFLTLFVNTLLNITIASRVLEDRLTRSACAAFIGDDNIIHGVVSDELMAARC
                     ATWMNMEVKIIDAVVSQKAPYFCGGFILYDTVAGTACRVADPLKRLFKLGKPLAAGDEQ
                     DDDRRRALADEVVRWQRTGLTDELEKAVHSRYEVQGISVVVMSMATFASSRSNFEKLRG
                     PVVTLYGGPK"
     RBS             4355..4363
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      7480..7500
                     /label="subgenomic promoter"
                     /note="subgenomic promoter"
                     /regulatory_class="promoter"
     CDS             7569..11312
                     /note="Structural polyprotein from Chikungunya virus
                     (strain 37997). Accession#: Q5XXP3"
                     /label="Structural polyprotein"
     3'UTR           11316..11881
     polyA_site      11882
     promoter        11997..12101
                     /label="AmpR promoter"
     CDS             12102..12959
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      13133..13721
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        13809..13826
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 atggctgcgt gagacacacg tagcctacca gtttcttact gctctactct gcttagcaag
       61 agacttgaga acccatcatg gatcccgtgt acgtggacat agacgccgac agcgcctttt
      121 taaaggccct gcagcgtgcg taccccatgt ttgaggtgga accaaggcag gtcacaccga
      181 atgaccatgc caatgctaga gcattctcgc atctagctat aaaactaata gagcaggaaa
      241 ttgatcccga ctcaaccatc ctggacatag gcagcgcgcc agcaaggagg atgatgtcgg
      301 ataggaagta ccactgcgtt tgccctatgc gcagcgcaga agaccctgag agactcgcca
      361 actacgcgag aaaactagca tctgccgcag gaaaagtctt ggacagaaac atctccgaaa
      421 aaattggaga tctacaagca gtaatggctg taccagacgc agaaacgccc acattctgct
      481 tgcacactga cgtctcatgt agacaaaggg cggacgtcgc tatataccag gatgtctacg
      541 ccgtgcatgc accaacatcg ctgtaccacc aggcgattaa aggagtccgt gtagcatact
      601 ggatagggtt tgatacaacc ccgttcatgt ataatgccat ggcaggtgca tacccctcgt
      661 actcgacaaa ctgggcagat gagcaggtgc tgaaggcaaa gaacatagga ttatgttcaa
      721 cagacctgac ggaaggtaga cgaggtaaat tgtctatcat gagaggaaaa aagatgaagc
      781 catgtgaccg cgtactgttc tcagtcgggt caacgcttta cccggagagc cgtaagcttc
      841 ttaagagttg gcacttacct tcagtgttcc atctaaaagg gaagctcagc ttcacgtgcc
      901 gctgtgatac agtggtttcg tgtgaaggct atgtcgttaa gagaataacg attagcccgg
      961 gcctctacgg taaaaccaca gggtacgcag taacccacca tgcagacgga ttcctaatgt
     1021 gcaaaacaac cgatacggta gatggcgaga gagtgtcatt ttcggtatgc acgtacgtac
     1081 ccgcaaccat ttgtgatcaa atgacaggta ttcttgccac ggaggttaca ccggaggatg
     1141 cacagaagct gctggtggga ctgaaccaga ggatagtggt caatggcaga acgcagagga
     1201 acacgaacac aatgaagaat tacttgcttc ctgtagttgc ccaagccctc agtaagtggg
     1261 caaaggaatg ccggaaagat atggaagatg aaaaactttt gggcatcaga gaaaggacac
     1321 tgacatgctg ctgcctttgg gcgttcaaga agcagaagac acacacggtc tacaagaggc
     1381 ctgacactca gtcaattcag aaagtcccag ccgaatttga cagctttgtg gtaccaagtc
     1441 tgtggtcatc tggactgtcg atcccgctac ggaccagaat caagtggctg ctaagcaaag
     1501 tgccaaagac tgatttgatc ccttacagcg gtgacgccaa agaagcccgc gacgctgaaa
     1561 aagaagcaga agaagaacga gaagcggagc taactcgcga ggcactacca ccactacagg
     1621 cggcacagga cgacgtccag gtcgaaattg acgtggaaca gctcgaagac agagctgggg
     1681 caggaataat tgaaactcca agaggagcta tcaaagtcac tgcccaacca acagaccacg
     1741 tcgtgggaga gtacttggta ctttccccgc agaccgtgtt acgaagccag aagctcagcc
     1801 tgatccacgc attggcggaa caagtgaaga catgcacaca cagcggacgg gcaggaaggt
     1861 acgcggtcga agcatatgac ggcagaatcc ttgtgccctc aggctatgca atatcacctg
     1921 aagacttcca gagcctgagc gaaagtgcga cgatggtgta caacgaaagg gagttcgtaa
     1981 ataggaaatt acaccatatc gcgttgcacg gaccagccct gaacactgac gaggagtcgt
     2041 acgagctggt aagggcagaa aggacagagc atgagtacgt ctatgatgtg gaccaaagaa
     2101 ggtgctgcaa gaaagaggag gcagccgggc tggtactggt cggcgacttg accaacccgc
     2161 cctaccatga gttcgcatat gaagggctga gaatccgccc cgcctgccca tacaagaccg
     2221 cagtaatagg ggtctttgga gtgccaggat ccggcaaatc agcaatcatt aagaacctag
     2281 ttaccaggca agacctagtg accagtggaa agaaagaaaa ctgccaagaa atctccaccg
     2341 acgtgatgcg acagaggaac ctggagatat ctgcacgcac ggtcgactca ctgctcttga
     2401 acggatgcaa tagaccagtc gacgtgttgt acgtcgacga agcttttgcg tgccattctg
     2461 gcacgctact tgctctgata gccttggtga gaccgaggca gaaagtcgtg ctatgcggtg
     2521 atccgaaaca gtgcggcttc ttcaatatga tgcagatgaa agttaactac aaccataaca
     2581 tctgcaccca agtgtaccat aaaagtattt ccaggcggtg tacactgcct gtgactgcca
     2641 ttgtgtcctc gttgcattac gaaggcaaaa tgcgcacaac aaatgagtac aacaagccaa
     2701 ttgtagtgga tactacaggc tcgacaaaac ccgaccccgg agaccttgtg ctaacatgtt
     2761 tcagagggtg ggttaagcaa ctgcaaattg actatcgtgg acacgaggtc atgacagcag
     2821 ctgcatctca ggggctaacc agaaaagggg tctatgccgt caggcaaaaa gttaatgaaa
     2881 acccccttta cgcatcaaca tcagagcacg tgaacgtgct actgacgcgt acggaaggca
     2941 aactagtatg gaagacactt tctggagacc catggataaa gacactgcag aacccgccga
     3001 aaggaaattt taaagcaaca attaaggaat gggaagtgga acatgcttca ataatggcgg
     3061 gtatctgtaa ccaccaagtg acctttgaca cgttccagaa taaagccaat gtctgctggg
     3121 cgaagagctt agtccccatc ctagaaacag cagggataaa attaaacgac aggcagtggt
     3181 cccagataat ccaggctttt aaagaagaca gagcatactc acccgaggtg gccctgaatg
     3241 agatatgcac gcgcatgtac ggggtagacc tggacagcgg actgttctct aaaccactgg
     3301 tgtccgtgca tcatgcggat aatcactggg acaacaggcc gggagggaag atgttcggat
     3361 tcaaccccga agcggcgtcc atactggaga ggaaataccc gtttacaaaa gggaagtgga
     3421 ataccaacaa gcaaatctgt gtgactacta ggaggattga agattttaac ccgaacacca
     3481 acattatacc tgccaacagg agattaccgc attcattggt ggccgaacat cgcccggtaa
     3541 aaggggagag gatggaatgg ttggtcaaca aaataaatgg ccaccatgtg ctcctggtca
     3601 gcggctacaa cctcgttctg cccactaaga gagtcacctg ggtggcgccg ctgggcattc
     3661 ggggagctga ctacacatac aacctagagt taggcctacc agcaacgctc ggtagatatg
     3721 acctagtgat tataaacatc cacacaccct ttcgcataca tcattaccaa cagtgcgtgg
     3781 atcacgcaat gaagctgcag atgctcggag gagactccct gagactgctc aagccgggtg
     3841 gttcattact gatcagggca tacggctacg cagacagaac aagcgaacga gtagtctgcg
     3901 tattgggacg caagtttcga tcatccagag cgttgaaacc gccgtgcgtc actagcaaca
     3961 ccgagatgtt tttcttgttc agcaactttg ataacggcag aaggaacttt acgacgcacg
     4021 taatgaacaa ccagctgaat gctgcttttg ttggtcaggc cacccgagca gggtgcgcac
     4081 cgtcgtaccg ggttaaacgc atggacatcg caaagaacga tgaagagtgt gtagtcaacg
     4141 ccgccaaccc tcgtgggcta ccaggcgatg gcgtctgtaa agcagtatac aaaaaatggc
     4201 cggagtcctt caagaacagt gcaacaccag tgggaaccgc aaagacagtc atgtgcggta
     4261 catacccggt aatccatgca gtaggaccta atttctcaaa ttactctgag tccgaaggag
     4321 accgggaatt ggcagctgct taccgagaag tcgctaagga ggtgactaga ctaggagtaa
     4381 acagcgtagc tataccgctc ctttccaccg gtgtgtactc tggagggaaa gacaggctga
     4441 ctcagtcact aaaccacctt tttacagcat tagactcaac tgatgcagat gtggttatct
     4501 actgccgcga caaggagtgg gagaagaaaa tagctgaggc catacaaatg aggacccaag
     4561 tggaattact agacgaacac atctctgtag actgcgatat catccgagtg caccctgaca
     4621 gcagtttggc aggtagaaaa gggtacagca ctacagaagg ttcactgtac tcctacttgg
     4681 aagggacacg gttccatcag acggcagtgg acatggcaga agtatacacc atgtggccaa
     4741 agcagacgga ggctaatgaa caagtttgct tgtacgcatt gggggaaagt atagaatcaa
     4801 tcaggcaaaa gtgcccagtg gatgacgcag atgcatcgtc gcccccaaaa accgtcccgt
     4861 gcctctgccg ttatgccatg acacccgaac gagtcaccag gcttcgtatg aaccatgtca
     4921 caagcataat agtatgctca tcattccccc ttccaaagta taaaatagaa ggagtgcaga
     4981 aagtcaagtg ttctaaagtg atgctgttcg accataacgt gccatcacgc gttagtccaa
     5041 gggaatataa atcgcctcag gagaccgcac aagaagtaag ttcgaccacg tcactgacgc
     5101 acagccaatt cgaccttagc gttgacggtg aggaactgcc cgctccgtct gacttggaag
     5161 ctgacgctcc gattccggaa ccaacaccag acgacagagc ggtacttact ttgcctccca
     5221 cgattgataa tttttcggct gtgtcagact gggtaatgaa taccgcgcca gtcgcaccac
     5281 ccagaagaag acgtgggaaa aacttgaatg tcacctgcga cgagagagaa gggaacgtac
     5341 ttcccatggc tagcgttcgg ttcttcagag cggatctgca ctccatcgta caggaaacgg
     5401 cagagatacg cgatacggcc gcgtccctcc aggcgcccct gagtgtcgct acagaaccga
     5461 atcaactgcc gatctcattt ggagcaccaa acgagacttt ccccataacg ttcggggatt
     5521 ttgatgaagg ggagattgaa agcttgtcct ctgagttact gacctttggg gacttctcgc
     5581 cgggcgaagt ggatgacctg acagacagcg actggtccac gtgttcagac acggacgacg
     5641 aattatgact agatagggca ggtgggtaca tattctcatc tgacaccggc cccggccacc
     5701 tgcaacagag gtctgtccgt cagacagtac tgccggtaaa taccttggag gaagttcagg
     5761 aggagaaatg ttacccacct aagttggatg aagtgaaaga gcagttgtta cttaagaaac
     5821 tccaggaaag tgcgtccatg gctaacagaa gcaggtacca atcccgcaaa gtagagaaca
     5881 tgaaagcaac aatagtccaa aggctgaagg gtggttgcaa actttattta atgtcggaga
     5941 ccccgaaagt tcctacctac cgaactacat atccggcacc agtgtactca cccccaatca
     6001 atatccgact gtccaacccc gagtctgctg tggcagcgtg caatgagttc ctagcaagga
     6061 actatccgac agttgcgtcg taccaaatca ccgatgagta cgatgcatac ctagacatgg
     6121 tggacgggtc ggaaagttgc cttgaccggg cgacgttcaa cccatcaaag cttagaagtt
     6181 atccaaaaca gcactcctac catgcaccca caatcagaag tgccgtacct tccccgttcc
     6241 agaacacgct gcagaacgta ctggctgctg ccacgaaaag aaattgcaac gtcacacaga
     6301 tgagagaact gcctactttg gattcagcgg tatttaatgt tgagtgcttt aaaaaatttg
     6361 cgtgcaatca agaatactgg aaggaatttg ccgccagccc tattaggata acgactgaga
     6421 acttgacaac ttatgtcaca aaactaaaag gaccaaaagc agcagcactg tttgccaaga
     6481 cacataacct gctaccactg caggaggtgc cgatggacag gtttactgta gacatgaaaa
     6541 gggacgtgaa ggtgactccg gggacgaagc acactgagga aagacctaaa gtgcaggtca
     6601 tacaggcagc cgaacctttg gcaacagcat atctgtgtgg gatccacaga gagttggtca
     6661 gaaggctgaa tgcagtcctt ctacctaatg tacacacgct gtttgacatg tctgccgagg
     6721 actttgacgc cattattgcc gcgcacttca agccggggga cgccgtattg gaaaccgata
     6781 tagcctcctt tgacaagagc caagacgact cattggcgct cactgctcta atgttgctag
     6841 aggatttggg ggtggatcat cccctgttgg acttgataga ggctgccttc ggggagatct
     6901 ccagctgcca cctaccgacg ggcacccgtt ttaagttcgg cgccatgatg aagtctggta
     6961 tgttcctaac cctgttcgtc aacacactgc taaacatcac catagccagc cgagtgctgg
     7021 aggaccgctt gacaaggtct gcgtgcgcgg ccttcatcgg cgacgacaat ataatacatg
     7081 gggttgtctc tgacgaactg atggcagcaa ggtgtgctac atggatgaac atggaagtga
     7141 agatcataga tgcggtcgtg tctcagaaag ccccgtactt ctgcggaggg tttatactgt
     7201 atgacacagt agcaggcacg gcctgcagag tggcagaccc gctaaagcgg ctgttcaagc
     7261 tgggcaaacc gctggcagcg ggagatgaac aagacgacga cagaagacgt gcactggctg
     7321 acgaagtggt tagatggcaa cgaacaggac taactgatga gctagaaaaa gcggtacact
     7381 ccaggtatga agtgcagggc atatctgtcg tggtaatgtc tatggccacc tttgcaagct
     7441 ctagatctaa ctttgagaag ctcagaggac ccgtcgtaac cctgtacggt ggtcctaaat
     7501 aggtacgcac tacagctacc tatttcgtca gaaaccaatc gcagctactt gcatacctac
     7561 cagctacaat ggagttcatc ccgacgcaaa ctttctataa cagaaggtac caaccccgac
     7621 cctgggcccc acgccctaca attcaagtaa ttagacctag accacgtcca cagaggcagg
     7681 ctgggcaact cgcccagctg atctccgcag tcaacaaatt gaccatgcgc gcggtacctc
     7741 aacagaagcc tcgcagaaat cggaaaaaca agaagcaaag gcagaagaag caggcgccgc
     7801 aaaacgaccc aaagcaaaag aagcaaccac cacaaaagaa gccggctcaa aagaagaaga
     7861 aaccaggccg tagggagaga atgtgcatga aaattgaaaa tgattgcatc ttcgaagtca
     7921 agcatgaagg caaagtgatg ggctacgcat gcctggtggg ggataaagta atgaaaccag
     7981 cacatgtgaa gggaactatc gacaatgccg atctggctaa actggccttt aagcggtcgt
     8041 ctaaatacga tcttgaatgt gcacagatac cggtgcacat gaagtctgat gcctcgaagt
     8101 ttacccacga gaaacccgag gggtactata actggcatca cggagcagtg cagtattcag
     8161 gaggccggtt cactatcccg acgggtgcag gcaagccggg agacagcggc agaccgatct
     8221 tcgacaacaa aggacgggtg gtggccatcg tcctaggagg ggccaacgaa ggtgcccgca
     8281 cggccctctc cgtggtgacg tggaacaaag acatcgtcac aaaaattacc cctgagggag
     8341 ccgaagagtg gagcctcgcc ctcccggtct tgtgcctgtt ggcaaacact acattcccct
     8401 gctctcagcc gccttgcaca ccctgctgct acgaaaagga accggaaagc accttgcgca
     8461 tgcttgagga caacgtgatg agacccggat actaccagct actaaaagca tcgctgactt
     8521 gctctcccca ccgccaaaga cgcagtacta aggacaattt taatgtctat aaagccacaa
     8581 gaccatatct agctcattgt cctgactgcg gagaagggca ttcgtgccac agccctatcg
     8641 cattggagcg catcagaaat gaagcaacgg acggaacgct gaaaatccag gtctctttgc
     8701 agatcgggat aaagacagat gacagccacg attggaccaa gctgcgctat atggatagcc
     8761 atacgccagc ggacgcggag cgagccggat tgcttgtaag gacttcagca ccgtgcacga
     8821 tcaccgggac catgggacac tttattctcg cccgatgccc gaaaggagag acgctgacag
     8881 tgggatttac ggacagcaga aagatcagcc acacatgcac acacccgttc catcatgaac
     8941 cacctgtgat aggtagggag aggttccact ctcgaccaca acatggtaaa gagttacctt
     9001 gcagcacgta cgtgcagagc accgctgcca ctgctgagga gatagaggtg catatgcccc
     9061 cagatactcc tgaccgcacg ctgatgacgc agcagtctgg caacgtgaag atcacagtta
     9121 atgggcagac ggtgcggtac aagtgcaact gcggtggctc aaacgaggga ctgacaacca
     9181 cagacaaagt gatcaataac tgcaaaattg atcagtgcca tgctgcagtc actaatcaca
     9241 agaattggca atacaactcc cctttagtcc cgcgcaacgc tgaactcggg gaccgtaaag
     9301 gaaagatcca catcccattc ccattggcaa acgtgacttg cagagtgcca aaagcaagaa
     9361 accctacagt aacttacgga aaaaaccaag tcaccatgct gctgtatcct gaccatccga
     9421 cactcttgtc ttaccgtaac atgggacagg aaccaaatta ccacgaggag tgggtgacac
     9481 acaagaagga ggttaccttg accgtgccta ctgagggtct ggaggtcact tggggcaaca
     9541 acgaaccata caagtactgg ccgcagatgt ctacgaacgg tactgctcat ggtcacccac
     9601 atgagataat cttgtactat tatgagctgt accccactat gactgtagtc attgtgtcgg
     9661 tggcctcgtt cgtgcttctg tcgatggtgg gcacagcagt gggaatgtgt gtgtgcgcac
     9721 ggcgcagatg cattacacca tatgaattaa caccaggagc cactgttccc ttcctgctca
     9781 gcctgctatg ctgcgtcaga acgaccaagg cggccacata ttacgaggct gcggcatatc
     9841 tatggaacga acagcagccc ctgttctggt tgcaggctct tatcccgctg gccgccttga
     9901 tcgtcctgtg caactgtctg aaactcttgc catgctgctg taagaccctg gcttttttag
     9961 ccgtaatgag catcggtgcc cacactgtga gcgcgtacga acacgtaaca gtgatcccga
    10021 acacggtggg agtaccgtat aagactcttg tcaacagacc gggttacagc cccatggtgt
    10081 tggagatgga gctacaatca gtcaccttgg aaccaacact gtcacttgac tacatcacgt
    10141 gcgagtacaa aactgtcatc ccctccccgt acgtgaagtg ctgtggtaca gcagagtgca
    10201 aggacaagag cctaccagac tacagctgca aggtctttac tggagtctac ccatttatgt
    10261 ggggcggcgc ctactgcttt tgcgacgccg aaaatacgca attgagcgag gcacatgtag
    10321 agaaatctga atcttgcaaa acagagtttg catcggccta cagagcccac accgcatcgg
    10381 cgtcggcgaa gctccgcgtc ctttaccaag gaaacaacat taccgtagct gcctacgcta
    10441 acggtgacca tgccgtcaca gtaaaggacg ccaagtttgt cgtgggccca atgtcctccg
    10501 cctggacacc ttttgacaac aaaatcgtgg tgtacaaagg cgacgtctac aacatggact
    10561 acccaccttt tggcgcagga agaccaggac aatttggtga cattcaaagt cgtacaccgg
    10621 aaagtaaaga cgtttatgcc aacactcagt tggtactaca gaggccagca gcaggcacgg
    10681 tacatgtacc atactctcag gcaccatctg gcttcaagta ttggctgaag gaacgaggag
    10741 catcgctaca gcacacggca ccgttcggtt gccagattgc gacaaacccg gtaagagctg
    10801 taaattgcgc tgtggggaac ataccaattt ccatcgacat accggatgcg gcctttacta
    10861 gggttgtcga tgcaccctct gtaacggaca tgtcatgcga agtaccagcc tgcactcact
    10921 cctccgactt tgggggcgtc gccatcatca aatacacagc tagcaagaaa ggtaaatgtg
    10981 cagtacattc gatgaccaac gccgttacca ttcgagaagc cgacgtagaa gtagagggga
    11041 actcccagct gcaaatatcc ttctcaacag ccctggcaag cgccgagttt cgcgtgcaag
    11101 tgtgctccac acaagtacac tgcgcagccg catgccaccc tccaaaggac cacatagtca
    11161 attacccagc atcacacacc acccttgggg tccaggatat atccacaacg gcaatgtctt
    11221 gggtgcagaa gattacggga ggagtaggat taattgttgc tgttgctgcc ttaattttaa
    11281 ttgtggtgct atgcgtgtcg tttagcaggc actaaaccga tgataaggca cgaaataact
    11341 aaatagcaaa agtagaaagt acataaccag gtatatgtgc cccttaagag gcacaatata
    11401 tatagctaag cactattaga tcaaagggct atacaacccc tgaatagtaa caaaacacaa
    11461 aaaccaataa aaatcataaa aagaaaaatc tcataaacag gtataagtgt cccctaagag
    11521 acacattgta tgtaggtagt aagtatagat caaagggcta tattaacccc tgaatagtaa
    11581 caaaacacaa aaacaataaa aactacaaaa tagaaaatct ataaacaaaa gtagttcaaa
    11641 gggctacaaa acccctgaat agtaacaaaa cataaaatgt aataaaaatt aagtgtgtac
    11701 ccaaaagagg tacagtaaga atcagtgaat atcacaattg gcaacgagaa gagacgtagg
    11761 tatttaagct tcctaaaagc agccgaactc actttgagac gtaggcatag cataccgaac
    11821 tcttccacta ttctccgaac ccacagggac gtaggagatg ttattttgtt tttaatattt
    11881 caaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agcggccgct taattaatcg
    11941 aggggaatta attcttgaag acgaaagggc caggtggcac ttttcgggga aatgtgcgcg
    12001 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
    12061 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
    12121 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
    12181 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
    12241 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
    12301 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
    12361 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
    12421 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
    12481 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
    12541 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
    12601 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
    12661 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
    12721 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
    12781 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    12841 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    12901 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    12961 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    13021 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    13081 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    13141 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    13201 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    13261 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
    13321 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    13381 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    13441 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    13501 aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg
    13561 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    13621 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    13681 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcgagct
    13741 cgtatggaca tattgtcgtt agaacgcggc tacaattaat acataacctt atgtatcata
    13801 cacaatcgat ttaggtgaca ctatag
//
