LOCUS       40924_10646       12806 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Plant binary expression vector pCHF3301, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12806)
  AUTHORS   Li JT, Yu G, Sun XH, Jia CG, Du Q, Li QY, Pan HY.
  TITLE     Modification of vectors for functional genomic analysis in plants
  JOURNAL   Genet. Mol. Res. 13 (3), 7815-7825 (2014)
  PUBMED    25299096
REFERENCE   2  (bases 1 to 12806)
  AUTHORS   Yu G, Sun X, Li J, Pan H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2013) Plant Pathology, College of Plant Science, 
            Jilin University, Xi'an Road 5333, Changchun, Jilin 130062, China
REFERENCE   3  (bases 1 to 12806)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12806)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genet. Mol.
            Res."; date: "2014"; volume: "13"; issue: "3"; pages: "7815-7825"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-JUN-2013) Plant Pathology, College of Plant Science, Jilin 
            University, Xi'an Road 5333, Changchun, Jilin 130062, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Lasergene v. 7.1
            Sequencing Technology :: 454
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          17..206
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             201..2024
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="LQNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQ
                     ESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWV
                     NNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQ
                     SYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELR
                     DADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELEVTAKSQTESDIYPLRVGIRSVAV
                     KGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAE
                     EMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKE
                     LIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHT
                     DTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAG
                     LHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIF
                     TRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     CDS             2031..2048
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      2083..2335
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2357..2381
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3681..4307
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4744..5808
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      5877..6071
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6415..6555
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6741..7329)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7419..8210)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8635..8659
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(8737..8911)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8921..9469)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(9514..10191)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    10382..10403
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10418..10448
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10456..10472
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10480..10496
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10519..11353
                     /label=CaMV35S(long)
                     /note="Cauliflower mosaic virus 35S promoter (long)"
     terminator      11399..12040
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(12053..12069)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        12446..12791
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct
       61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga
      121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt
      181 gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaaaccccaa
      241 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg
      301 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag
      361 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt
      421 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg
      481 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg
      541 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac
      601 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga
      661 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg
      721 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg
      781 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca
      841 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag
      901 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc
      961 tctatgaact cgaagtcaca gccaaaagcc agacagagtc tgatatctac ccgcttcgcg
     1021 tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct
     1081 actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg
     1141 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct
     1201 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga
     1261 ttgatgaaac tgctgctgtc ggctttcagc tgtctttagg cattggtttc gaagcgggca
     1321 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact
     1381 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga
     1441 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg
     1501 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct
     1561 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt
     1621 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac
     1681 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata
     1741 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat
     1801 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat
     1861 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag
     1921 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga
     1981 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaagctagc caccaccacc
     2041 accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg
     2101 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
     2161 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
     2221 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
     2281 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
     2341 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
     2401 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
     2461 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg
     2521 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca
     2581 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc
     2641 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa
     2701 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt
     2761 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat
     2821 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg
     2881 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga
     2941 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc
     3001 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt
     3061 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg
     3121 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct
     3181 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc
     3241 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg
     3301 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga
     3361 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt
     3421 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac
     3481 gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc
     3541 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta
     3601 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa
     3661 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag
     3721 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc
     3781 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc
     3841 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg
     3901 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg
     3961 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg
     4021 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac
     4081 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg
     4141 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct
     4201 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg
     4261 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg
     4321 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag
     4381 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt
     4441 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac
     4501 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat
     4561 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag
     4621 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg
     4681 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt
     4741 gacggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg
     4801 gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc
     4861 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca
     4921 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt
     4981 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc
     5041 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg
     5101 cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta
     5161 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac
     5221 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc
     5281 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac
     5341 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt
     5401 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc
     5461 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg
     5521 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc
     5581 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa
     5641 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg
     5701 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta
     5761 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag
     5821 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg
     5881 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg
     5941 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa
     6001 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg
     6061 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt
     6121 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc
     6181 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg
     6241 ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg
     6301 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     6361 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     6421 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
     6481 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     6541 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     6601 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     6661 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     6721 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6781 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6841 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6901 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6961 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7021 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7081 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7141 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     7201 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7261 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7321 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7381 tcacgttaag ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata
     7441 taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca
     7501 tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac
     7561 ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca
     7621 aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc
     7681 gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt
     7741 tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg
     7801 tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac
     7861 tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa
     7921 aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc
     7981 aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc
     8041 acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt
     8101 tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg
     8161 tatttttcga tcagtttttt caattccggt gatattctca ttttagccat ttattatttc
     8221 cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact
     8281 ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt
     8341 tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat
     8401 cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc
     8461 gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa
     8521 agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt
     8581 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag
     8641 gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt
     8701 tttaatgtac tgaattaacg ccgaattaat tcgggggatc tggattttag tactggattt
     8761 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
     8821 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc
     8881 tgggaactac tcacacatta ttatggagaa actcgagtca aatctcggtg acgggcagga
     8941 ccggacgggg cggtaccggc aggctgaagt ccagctgcca gaaacccacg tcatgccagt
     9001 tcccgtgctt gaagccggcc gcccgcagca tgccgcgggg ggcatatccg agcgcctcgt
     9061 gcatgcgcac gctcgggtcg ttgggcagcc cgatgacagc gaccacgctc ttgaagccct
     9121 gtgcctccag ggacttcagc aggtgggtgt agagcgtgga gcccagtccc gtccgctggt
     9181 ggcgggggga gacgtacacg gtcgactcgg ccgtccagtc gtaggcgttg cgtgccttcc
     9241 aggggcccgc gtaggcgatg ccggcgacct cgccgtccac ctcggcgacg agccagggat
     9301 agcgctcccg cagacggacg aggtcgtccg tccactcctg cggttcctgc ggctcggtac
     9361 ggaagttgac cgtgcttgtc tcgatgtagt ggttgacgat ggtgcagacc gccggcatgt
     9421 ccgcctcggt ggcacggcgg atgtcggccg ggcgtcgttc tgggctcatg gtagactcga
     9481 gagagataga tttgtagaga gagactggtg atttcagcgt gtcctctcca aatgaaatga
     9541 acttccttat atagaggaag gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg
     9601 tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt
     9661 tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct
     9721 tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct
     9781 actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat
     9841 attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata
     9901 ttcttggagt agacgagagt gtcgtgctcc accatgttat cacatcaatc cacttgcttt
     9961 gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct
    10021 ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg
    10081 catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg
    10141 caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt
    10201 tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc
    10261 atgttggcaa gctgctctag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    10321 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    10381 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    10441 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    10501 attacgaatt cggtccccag attagccttt tcaatttcag aaagaatgct aacccacaga
    10561 tggttagaga ggcttacgca gcaggtctca tcaagacgat ctacccgagc aataatctcc
    10621 aggaaatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact
    10681 gcatcaagaa cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga
    10741 cgattcaagg cttgcttcac aaaccaaggc aagtaataga gattggagtc tctaaaaagg
    10801 tagttcccac tgaatcaaag gccatggagt caaagattca aatagaggac ctaacagaac
    10861 tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga
    10921 aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
    10981 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
    11041 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
    11101 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
    11161 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
    11221 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
    11281 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
    11341 tggagagaac acgggggacg agctcggtac ccggggatcc tctagagtcg acctgcagag
    11401 ctttcgttcg tatcatcggt ttcgacaacg ttcgtcaagt tcaatgcatc agtttcattg
    11461 cgcacacacc agaatcctac tgagttcgag tattatggca ttgggaaaca tgtttttctt
    11521 gtaccatttg ttgtgcttgt aatttactgt gttttttatt cggttttcgc tatcgaactg
    11581 tgaaatggaa atggatggag aagagttaat gaatgatatg gtccttttgt tcattctcaa
    11641 attaatatta tttgtttttt ctcttatttg ttgtgtgttg aatttgaaaa tataagagat
    11701 atgcaaacat tttgttttga gtaaaaatgt gtcaaatcgt ggcctctaat gaccgaagtt
    11761 aatatgagga gtaaaacact tgtagttgta ccattatgct tattcactag gcaacaaata
    11821 tattttcaga cctagaaaag ctgcaaatgt tactgaatac aagtatgtcc tcttgtgttt
    11881 tagacattta tgaactttcc tttatgtaat tttccagaat ccttgtcaga ttctaatcat
    11941 tgctttataa ttatagttat actcatggat ttgtagttga gtatgaaaat attttttaat
    12001 gcattttatg acttgccaat tgattgacaa catgcatcaa tcgaagcttg gcactggccg
    12061 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
    12121 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
    12181 aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat cagattgtcg
    12241 tttcccgcct tcagtttagc ttcatggagt caaagattca aatagaggac ctaacagaac
    12301 tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga
    12361 aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
    12421 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
    12481 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
    12541 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
    12601 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
    12661 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
    12721 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
    12781 tggagagaac acgggggact cttgac
//
