LOCUS       62056_6275        6412 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6412)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1075..1432
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1497..1913
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     misc_feature    1923..2296
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     CDS             2431..3096
                     /codon_start=1
                     /label=mKeima-Red
                     /note="monomeric Keima-Red fluorescent protein, also known
                     as mKeima"
                     /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV
                     TKGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTV
                     SNDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK
                     LEGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG
                     "
     misc_feature    3123..3699
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     LTR             3819..4412
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     rep_origin      complement(4714..5302)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5479..6336)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atcacgaggc cctttcgtct tcaagaacag ctttgctctt aggagtttcc taatacatcc
       61 caaactcaaa tatataaagc atttgacttg ttctatgccc tagggggcgg ggggaagcta
      121 agccagcttt ttttaacatt taaaatgtta attccatttt aaatgcacag atgtttttat
      181 ttcataaggg tttcaatgtg catgaatgct gcaatattcc tgttaccaaa gctagtataa
      241 ataaaaatag ataaacgtgg aaattactta gagtttctgt cattaacgtt tccttcctca
      301 gttgacaaca taaatgcgct gctgagaagc cagtttgcat ctgtcaggat caatttccca
      361 ttatgccagt catattaatt actagtcaat tagttgattt ttatttttga catatacatg
      421 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
      481 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca gatggaacag
      541 ctgaatatgg gccaaagcgg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      601 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      661 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      721 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      781 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      841 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
      901 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      961 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
     1021 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
     1081 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
     1141 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
     1201 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
     1261 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag
     1321 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
     1381 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg
     1441 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatatg ggcccgggcc
     1501 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     1561 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     1621 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     1681 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     1741 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     1801 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     1861 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
     1921 ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgaca
     1981 tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc
     2041 agcacgaagt ctggagacct ctggcggcac gtaccaagaa caactggacc gaccggtggt
     2101 acctcaccct taccgagtcg gcgacacagt gtgggtccgc cgacaccaga ctaagaacct
     2161 agaacctcgc tggaaaggac cttacacagt cctgctgacc acccccaccg ccctcaaagt
     2221 agacggcatc gcagcttgga tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg
     2281 accatcctct agactgccgg atccacgcgt atgcatctcg aggccaccat ggacagcaaa
     2341 ggttcgtcgc agaaagcagg gtcccgcctg ctcctgctgc tggtggtgtc aaatctactc
     2401 ttgtgccagg gtgtggtctc cggatccgcc atggtgagcg tgatcgccaa gcagatgacc
     2461 tacaaggtgt acatgagcgg caccgtgaac ggccactact tcgaggtgga gggcgacggc
     2521 aagggcaagc cctacgaggg cgagcagacc gtgaagctga ccgtgaccaa gggtggcccc
     2581 ctgcccttcg cctgggacat cctgagcccc cagctccagt acggcagcat ccccttcacc
     2641 aagtaccccg aggacatccc cgactacttc aagcagagct tccccgaggg ctacacctgg
     2701 gagcgcagca tgaacttcga ggacggcgcc gtgtgcaccg tgagcaacga cagcagcatc
     2761 cagggcaact gcttcatcta caacgtgaag atcagcggcg agaacttccc ccccaacggc
     2821 cccgtgatgc agaagaagac ccagggctgg gagcccagca ccgagcgcct gttcgcccgc
     2881 gacggaatgc tgatcggcaa cgactacatg gccctgaagc tggagggcgg cggccactac
     2941 ctgtgcgagt tcaagagcac ctacaaggcc aagaagcccg tgaggatgcc cggccgccac
     3001 gagatcgacc gcaagctgga cgtgaccagc cacaaccgcg actacaccag cgtggagcag
     3061 tgcgagatcg ccatcgcccg ccactccctg ctgggcaaag atgagctcta aggatccgtc
     3121 gacccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     3181 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     3241 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     3301 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     3361 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     3421 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     3481 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     3541 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     3601 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     3661 ttttcctttg aaaaacacga tgataatatg gccacaacca tggggcgcgc cgtatactga
     3721 tcaatcgatt caattgcacc ggtgcctgca ggccatcccc ttggcgtctc ctcgacgata
     3781 aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg
     3841 tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga
     3901 atagagaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg gccaaacagg
     3961 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg aacagctgaa
     4021 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
     4081 gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca gatgtttcca
     4141 gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct
     4201 tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc
     4261 actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac
     4321 cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt
     4381 gattgactac ccgtcagcgg gggtctttca ttcacatgca gcatgtatca aaattaattt
     4441 ggtttttttt cttaagtatt tacattaaat ggccatagtt gcattaatga atcggccaac
     4501 gcgcggggag aggcggtttg cgtattggcg ctcttccgct tcctcgctca ctgactcgct
     4561 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     4621 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     4681 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4741 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4801 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4861 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4921 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4981 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     5041 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     5101 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     5161 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     5221 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     5281 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     5341 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     5401 ctagatcctt ttaaattaaa aatgaagttt gcgcaaatca atctaaagta tatatgagta
     5461 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     5521 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     5581 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     5641 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     5701 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     5761 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     5821 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     5881 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     5941 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     6001 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     6061 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
     6121 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     6181 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     6241 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     6301 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     6361 aagcatttat cagggttatt gtctcatgac attaacctat aaaaataggc gt
//
