LOCUS       V016292                 6490 bp    DNA     circular SYN 01-FEB-2022
DEFINITION  Exported.
ACCESSION   V016292
VERSION     V016292
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Ruf S, Forner J, Hasse C, Kroop X, Seeger S, Schollbach L, Schadach
            A, Bock R.
  TITLE     High-efficiency generation of fertile transplastomic Arabidopsis
            plants
  JOURNAL   Nat Plants 5 (3), 282-289 (2019)
   PUBMED   30778165
REFERENCE   2  (bases 1 to 6490)
  AUTHORS   Forner J, Hasse C, Ruf S, Bock R.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2018) Organelle Biology, Biotechnology and
            Molecular Ecophysiology, Max Planck Institute of Molecular Plant
            Physiology, Am Muehlenberg 1, Potsdam, Brandenburg D-14476, Germany
REFERENCE   3  (bases 1 to 6490)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat
            Plants"; date: "2019"; volume: "5"; issue: "3"; pages: "282-289"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-JUL-2018) Organelle Biology, Biotechnology and Molecular
            Ecophysiology, Max Planck Institute of Molecular Plant Physiology,
            Am Muehlenberg 1, Potsdam, Brandenburg D-14476, Germany"
FEATURES             Location/Qualifiers
     source          1..6490
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            1..521
                     /gene="psaB"
                     /label="psaB"
     CDS             1..521
                     /codon_start=3
                     /transl_table=11
                     /gene="psaB"
                     /product="PSAB"
                     /label="psaB"
                     /protein_id="QBG49732.1"
                     /translation="GRGGTCDISAWDAFYLAVFWMLNTIGWVTFYWHWKHITLWQGNVS
                     QFNESSTYLMGWLRDYLWLNSSQLINGYNPFGMNSLSVWAWMFLFGHLVWATGFMFLIS
                     WRGYWQELIETLAWAHERTPLANLIRWKDKPVALSIVQARLVGLAHFSVGYIFTYAAFL
                     IASTSGKFG"
     CDS             656..955
                     /gene="rps14"
                     /label="Small ribosomal subunit protein uS14c"
                     /note="Small ribosomal subunit protein uS14c from
                     Arabidopsis thaliana. Accession#: P56804"
     tRNA            1119..1192
                     /product="tRNA-Met"
                     /label="trnfM"
                     /note="trnfM"
     regulatory      1302..1451
                     /label="Zea mays clpP promoter"
                     /note="Zea mays clpP promoter"
                     /regulatory_class="promoter"
     CDS             1453..2241
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     regulatory      2252..2401
                     /label="Nicotiana tabacum rps16 terminator"
                     /note="Nicotiana tabacum rps16 terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(2417..2433)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     tRNA            complement(2493..2563)
                     /product="tRNA-Gly"
     CDS             complement(3117..3302)
                     /gene="psbZ"
                     /label="Photosystem II reaction center protein Z"
                     /note="Photosystem II reaction center protein Z from
                     Nicotiana tabacum. Accession#: P09974"
     promoter        complement(3641..3659)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3669..3685)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      3827..4282
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4308..4412
                     /label="AmpR promoter"
     CDS             4413..5270
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5444..6032
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    6320..6341
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6356..6386
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6394..6410
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6418..6434
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6455..6473
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 cgggacgagg tggtacttgt gatatttcgg cttgggacgc attttatttg gcagttttct
       61 ggatgttaaa tactattgga tgggttactt tttattggca ttggaaacac atcacattat
      121 ggcagggtaa cgtttcacag tttaatgaat cttccactta tttgatggga tggttaagag
      181 attatctatg gttaaactct tcacaactta tcaatggata taacccgttt ggtatgaata
      241 gtttatcagt ctgggcatgg atgttcttat ttgggcatct tgtttgggct acaggattta
      301 tgttcttaat ttcctggcgt ggttattggc aggaattgat tgaaacttta gcgtgggctc
      361 atgaacgcac acctttggca aatttgattc gctggaaaga taaaccagta gctctttcaa
      421 tcgtgcaagc aagattggtt ggattagctc acttttctgt aggttatata ttcacttatg
      481 cggctttctt gattgcctcc acatcgggca aattcggtta attatttatg tattctatcc
      541 gcaatcatat atttttttta ataaaaaaaa tataggtatg cactcttata tttatttcta
      601 catctaggat ccgatttgta tcattatcat tgataataac aggaactgaa gcattatggc
      661 aaagaaaagt ttgatttata gggagaagaa gaggcaaaaa ttggaaaaaa aatatcattt
      721 gattcgtcga tcctcaaaaa aggaaataag taagattccg tcgctaagtg agaaatggaa
      781 aattcatgga aaattacaat ccccaccgcg taatagtgca cctacacgtc ttcatcgacg
      841 ttgcttttcg accggaagac cgagagctaa ctatcgagac tttggactat ctggacacat
      901 ccttcgggaa atggttcagg catgtttgtt gccaggggca acaagatcaa gctggtaagg
      961 atttaagtat cccttaattt ttctattttt ttctatgatc gatgaggccc ctttaccatt
     1021 ctgtctaaat aggctattct atttgtacag atatggaaga ggggctcctt caattattct
     1081 ttgtttatta gagtttaatc caagtttttt tgtttcagcg cggggtagag cagtttggta
     1141 gctcgcaagg ctcataacct tgaggtcacg ggttcaaatc ctgtctccgc aacttttttt
     1201 tttcaagaaa tgtaagtttg attctactaa caagtcataa attaatacgt gtcttttggg
     1261 acgcgaggaa aaaattacaa tatttcccgg agctcggatc ctctatgtat taatagaatc
     1321 tatagtattc ttatagaata agaaaaaaaa aatgaagata ataaactgcg gattctttct
     1381 ttctcttcca ttcttacgtt tccatattaa agtgtagttt ttttacttaa atttaaagaa
     1441 ggagatatac ccatggggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     1501 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     1561 gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta
     1621 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     1681 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     1741 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     1801 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     1861 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     1921 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     1981 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     2041 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     2101 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     2161 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag
     2221 atcaccaagg tagtcggcaa ataatgtcta gagaaattca attaaggaaa taaattaagg
     2281 aaatacaaaa aggggggtag tcatttgtat ataactttgt atgacttttc tcttctattt
     2341 ttttgtattt cctccctttc cttttctatt tgtatttttt tatcattgct tccattgaat
     2401 tcgatatcaa gcttatcgat accgtcgacc tcgagggggg gcccggtaca actataccga
     2461 atattttctc tttttgaatc catttatatt tgggcggata gcgggaatcg aacccgcgtc
     2521 ttctccttgg caaggagaaa ttttaccatt cgactatatc cgcatttttt gcccttcttt
     2581 ataagaaatt tatggataat atttgttcaa agctagacga gatacactct ttggtttggg
     2641 gtcatttcat aaaattctac caccaaataa attcaattaa caattctatt aatatatata
     2701 tatatattat aaatatatat attaataata tatattatat ttattctata tattaatata
     2761 ttaatattaa ttcttgaata ttaatttaat ttaatattga attccagatt caagaatata
     2821 ttattcttat atttccataa aatattatat tctatattga cttgatatca gatctcaatt
     2881 ttttattcgt tttttttatt tatttttttt tattactatt tcatatttag gaactatttc
     2941 atatttagta actatttatt aatcttttat tcatatttca ttcaagttcc ataagttcga
     3001 ccccccctct aattttttct ttatttgcat tttatggact tatattgaat ttgaatgtgt
     3061 aattcaagga atgggagggg gtcatctcat ttttggatct gcaacgaatg aattcaagag
     3121 ataagagaat taaggatacc caccaagaag actaatccaa tccataaaga tgtaccagaa
     3181 aatacaacat ttttgttact cgaccaacca tcaggagacg caaatacaac gggtacacta
     3241 atcagtaaga ttgatgaagt aataattaat gcaaaaactg ccaattggaa agcaatagtc
     3301 atgtttttaa tcctccaagc tattaacaaa agaatataca ccatttaatc ctcttaccca
     3361 ctaaaaattt ttaataaata aagggatttt atcatgaatc cgttgattcg agatgactta
     3421 gttccaattt ctttttacca cttttagtct attcggacat gaagttaaag gttctttttt
     3481 acttcacaaa cgggtatact ggatcgcgta tctcatttat ttatatagcc agatttatag
     3541 acctattaaa gaaagtcaca ccctatatct ttctctacat agtgatcgta ttaactcata
     3601 gggctatgtt ctatttggcg aaggtacgta cccaattcgc cctatagtga gtcgtattac
     3661 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     3721 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     3781 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     3841 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     3901 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     3961 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     4021 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     4081 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     4141 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     4201 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     4261 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4321 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4381 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     4441 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4501 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4561 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4621 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     4681 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     4741 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     4801 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     4861 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     4921 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     4981 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     5041 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     5101 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     5161 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     5221 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     5281 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5341 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5401 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5461 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5521 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5581 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5641 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5701 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5761 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5821 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5881 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5941 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6001 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6061 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     6121 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     6181 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     6241 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     6301 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     6361 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     6421 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     6481 agctggagat
//
