LOCUS       40924_10621        7125 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCH110, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7125)
  AUTHORS   Kitts PA.
  TITLE     CLONTECH Vectors On Disc version 1.3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7125)
  AUTHORS   Hall CV, Jacob PE, Ringold GM, Lee F.
  TITLE     Expression and regulation of Escherichia coli lacZ gene fusions in 
            mammalian cells
  JOURNAL   J. Mol. Appl. Genet. 2 (1), 101-109 (1983)
  PUBMED    6302193
REFERENCE   3  (bases 1 to 7125)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 
            1020 East Meadow Circle, Palo Alto, CA 94303, USA
REFERENCE   4  (bases 1 to 7125)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7125)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "J. Mol. 
            Appl. Genet."; date: "1983"; volume: "2"; issue: "1"; pages: 
            "101-109"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East 
            Meadow Circle, Palo Alto, CA 94303, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     This sequence has been compiled from information in the sequence 
            databases, published literature and other sources. This vector is no
            longer available from CLONTECH and CLONTECH will not update or 
            revise this sequence.
FEATURES             Location/Qualifiers
     source          1..7125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             297..3341
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     polyA_signal    3608..3742
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4595..4699
                     /label=AmpR promoter
     CDS             4700..5557
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5731..6319
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(6505..6645)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     promoter        6796..7125
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 aagcttggga tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg
       61 tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt
      121 tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca
      181 ttattgccgt aagccgtggc ggtctggtac cggtgggtga agaccagaaa cagcacctcg
      241 aactgagccg cgatattgcc cagcgtttca acgcgctgta tggcgagatc gatcccgtcg
      301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
      361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
      421 agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc
      481 cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact
      541 ggcagatgca cggttacgat gcgcccatct acaccaacgt aacctatccc attacggtca
      601 atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg
      661 atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt aactcggcgt
      721 ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg
      781 aatttgacct gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc
      841 gttggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc ggcattttcc
      901 gtgacgtctc gttgctgcat aaaccgacta cacaaatcag cgatttccat gttgccactc
      961 gctttaatga tgatttcagc cgcgctgtac tggaggctga agttcagatg tgcggcgagt
     1021 tgcgtgacta cctacgggta acagtttctt tatggcaggg tgaaacgcag gtcgccagcg
     1081 gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca
     1141 cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg aatctctatc
     1201 gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa gcctgcgatg
     1261 tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc aagccgttgc
     1321 tgattcgagg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc
     1381 agacgatggt gcaggatatc ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt
     1441 cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg
     1501 tggatgaagc caatattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc
     1561 cgcgctggct accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc
     1621 acccgagtgt gatcatctgg tcgctgggga atgaatcagg ccacggcgct aatcacgacg
     1681 cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg
     1741 gagccgacac cacggccacc gatattattt gcccgatgta cgcgcgcgtg gatgaagacc
     1801 agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta cctggagaga
     1861 cgcgcccgct gatcctttgc gaatacgccc acgcgatggg taacagtctt ggcggtttcg
     1921 ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc gtctgggact
     1981 gggtggatca gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg
     2041 gtgattttgg cgatacgccg aacgatcgcc agttctgtat gaacggtctg gtctttgccg
     2101 accgcacgcc gcatccagcg ctgacggaag caaaacacca gcagcagttt ttccagttcc
     2161 gtttatccgg gcaaaccatc gaagtgacca gcgaatacct gttccgtcat agcgataacg
     2221 agctcctgca ctggatggtg gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc
     2281 tggatgtcgc tccacaaggt aaacagttga ttgaactgcc tgaactaccg cagccggaga
     2341 gcgccgggca actctggctc acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag
     2401 aagccgggca catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc
     2461 tccccgccgc gtcccacgcc atcccgcatc tgaccaccag cgaaatggat ttttgcatcg
     2521 agctgggtaa taagcgttgg caatttaacc gccagtcagg ctttctttca cagatgtgga
     2581 ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg
     2641 ataacgacat tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct
     2701 ggaaggcggc gggccattac caggccgaag cagcgttgtt gcagtgcacg gcagatacac
     2761 ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca gcatcagggg aaaaccttat
     2821 ttatcagccg gaaaacctac cggattgatg gtagtggtca aatggcgatt accgttgatg
     2881 ttgaagtggc gagcgataca ccgcatccgg cgcggattgg cctgaactgc cagctggcgc
     2941 aggtagcaga gcgggtaaac tggctcggat tagggccgca agaaaactat cccgaccgcc
     3001 ttactgccgc ctgttttgac cgctgggatc tgccattgtc agacatgtat accccgtacg
     3061 tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat ggcccacacc
     3121 agtggcgcgg cgacttccag ttcaacatca gccgctacag tcaacagcaa ctgatggaaa
     3181 ccagccatcg ccatctgctg cacgcggaag aaggcacatg gctgaatatc gacggtttcc
     3241 atatggggat tggtggcgac gactcctgga gcccgtcagt atcggcggaa ttccagctga
     3301 gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa ccgggcaggc
     3361 catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatttaaaa aacacaaact
     3421 tttggatgtt cggtttattc tttttctttt acttttttat catgggagcc tacttcccgt
     3481 ttttcccgat ttggctacat gacatcaacc atatcagcaa aagtgatacg ggtattattt
     3541 ttgccgctat ttctctgttc tcgctattat tccaaccgct gtttggtctg ctttctgaca
     3601 aactcggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3661 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3721 tgtatcttat catgtctgga tccccaggaa gctcctctgt gtcctcataa accctaacct
     3781 cctctacttg agaggacatt ccaatcatag gctgcccatc caccctctgt gtcctcctgt
     3841 taattaggtc acttaacaaa aaggaaattg ggtaggggtt tttcacagac cgctttctaa
     3901 gggtaatttt aaaatatctg ggaagtccct tccactgctg tgttccagaa gtgttggtaa
     3961 acagcccaca aatgtcaaca gcagaaacat acaagctgtc agctttgcac aagggcccaa
     4021 caccctgctc atcaagaagc actgtggttg ctgtgttagt aatgtgcaaa acaggaggca
     4081 cattttcccc acctgtgtag gttccaaaat atctagtgtt ttcattttta cttggatcag
     4141 gaacccagca ctccactgga taagcattat ccttatccaa aacagccttg tggtcagtgt
     4201 tcatctgctg actgtcaact gtagcatttt ttggggttac agtttgagca ggatatttgg
     4261 tcctgtagtt tgctaacaca ccctgcagct ccaaaggttc cccaccaaca gcaaaaaaat
     4321 gaaaatttga cccttgaatg ggttttccag caccattttc atgagttttt tgtgtccctg
     4381 aatgcaagtt taacatagca gttaccccaa taacctcagt tttaacagta acagcttccc
     4441 acatcaaaat atttccacag gttaagtcct catttaaatt aggcaaagga attcttgaag
     4501 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4561 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4621 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4681 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4741 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4801 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4861 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4921 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     4981 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5041 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5101 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5161 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5221 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5281 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5341 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5401 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5461 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5521 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5581 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5641 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5701 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5761 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5821 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     5881 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5941 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6001 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6061 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6481 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     6541 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     6601 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     6661 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     6721 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     6781 aggcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     6841 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     6901 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     6961 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7021 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7081 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaa
//
