LOCUS       40924_10576        6241 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pCGL0482, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Reyes O, Guyonvarch A, Bonamy C, Salti V, David F, Leblon G.
  TITLE     'Integron'-bearing vectors: a method suitable for stable chromosomal
            integration in highly restrictive corynebacteria
  JOURNAL   Gene 107 (1), 61-68 (1991)
  PUBMED    1660430
REFERENCE   2  (bases 1 to 6241)
  AUTHORS   Ankri S, Reyes O, Leblon G.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-1998) Laboratoire de Biologie Moleculaire des 
            Corynebacteries, Institut de Genetique et Microbiologie URA 2225, 
            Universite de Paris XI, Orsay F-91405 Orsay cedex, France
REFERENCE   3  (bases 1 to 6241)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6241)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1991"; volume: "107"; issue: "1"; pages: "61-68"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-SEP-1998) Laboratoire de Biologie Moleculaire des 
            Corynebacteries, Institut de Genetique et Microbiologie URA 2225, 
            Universite de Paris XI, Orsay F-91405 Orsay cedex, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     72..88
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(450..1106)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(1107..1209)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             complement(2338..3543)
                     /codon_start=1
                     /gene="repBl1"
                     /product="replicase"
                     /label=repBl1
                     /note="minimally known pBl1 region able to promote
                     replication"
                     /protein_id="AAD08689.1"
                     /translation="MYKITNSKALAGCHRWRRDEAVAVSWSSNGASQFEGLQNSHSRWG
                     SPLAELEVMGERRIELAIATKNHLAAGGALMMFVGTVRHNRSQSFAQVEAGIKTAYSSM
                     VKTSQWKKERARYGVEHTYSDYEVTDSWANGWHLHRNMLLFLDRPLSDDELKAFEDSMF
                     SRWSAGVVKAGMDAPLREHGVKLDQVSTWGGDAAKMATYLAKGMSQELTGSATKTASKG
                     SYTPFQMLDMLADQSDAGEDMDAVLVARWREYEVGSKNLRSSWSRGAKRALGIDYIDAD
                     VRREMEEELYKLAGLEAPERVESTRVAVALVKPDDWKLIQSDFAVRQYVLDCVDKAKDV
                     AAAQRVANEVLASLGVDSTPCMIVMDDVDLDAVLPTHGDATKRDLNAAVFAGNEQTILR
                     TH"
     gene            complement(2338..3543)
                     /gene="repBl1"
                     /label=repBl1
     rep_origin      complement(5130..5664)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     rep_origin      5732..6160
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gctaaccgtt tttatcaggc tctgggaggc agaataaatg atccgtcgag ctccaccgcg
       61 gtggcggccg ctctagaact agtggatccc ccgggctgca ggcctcgaga tctccatgga
      121 cgcgtgacgt cgacggtatc gtagcgctaa ccgtttttat caggctctgg gaggcagaat
      181 aaatgatcat atcgtcaatt attacctcca cggggagagc ctgagcaaac tggcctcagg
      241 catttgagaa gcacacggtc acactgcttc cggtagtcaa taaaccggta aaccagcaat
      301 agacataagc ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt
      361 cgaatttctg ccattcatcc gcttattatc acttattcag gcgtagcacc aggcgtttaa
      421 gggcaccaat aactgcctta aaaaaattac gccccgccct gccactcatc gcagtactgt
      481 tgtaattcat taagcattct gccgacatgg aagccatcac agacggcatg atgaacctga
      541 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
      601 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
      661 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
      721 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
      781 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
      841 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa ttccggatga
      901 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
      961 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     1021 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     1081 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
     1141 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     1201 tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg
     1261 acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta ttcggcgcaa
     1321 agtgcgtcgg gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc
     1381 tccagtggct tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg
     1441 taacggcaaa agcaccgccg gacatcagcg atgataagct gtcaaacatg agaattacaa
     1501 cttatatcgt atggggctga cttcaggtgc tacatttgaa gagataaatt gcactgaaat
     1561 ctagagcggc cgccaccgcg gtggagctcg acggatcaga tcatatcgtc aattattacc
     1621 tccacgggga gagcctgagc aaactggcct caggcatttg agaagcacac ggtcacactg
     1681 cttccggtag tcaataaacc ggtaaaccag caatagacat aagcggctat ttaacgaccc
     1741 tgccctgaac cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc atccgcttat
     1801 tatcacttat tcaggcgtag caccaggcgt ttaagggcac caataactgc cttaaaaaaa
     1861 ttacgccccg ccctgccact catcgcagta acgtcaacaa ccacccccgc aggcgttaag
     1921 ttgccccgcc aacagaaagg aaaccaacac gaaacaacaa cacaaaaagg tttcacagaa
     1981 aaaagcgtat gcgctaacgt atgccccgca gcacggcaaa acgcgcgtta agccctagcc
     2041 cacgcgcgcg taggtattac tcatgcccac tatggtgtgc acactgccca ctacggtgtg
     2101 caatctattc acgatgccac ccccagatac agtgaagccc cgccaatccg aactagatca
     2161 gatcaacggg gcaacccatt gtccccagct ttgattagga gccaggcaca taacagcatg
     2221 acagttccat tcctgatgaa atcagccatt gtcaacaaca agacccatca tagtttgccc
     2281 ccgcgacatt gaccataaat tcatcgcaca aaatatcgaa cggggtttat gccgctttta
     2341 gtgggtgcga agaatagtct gctcattacc cgcgaacacc gccgcattca gatcacgctt
     2401 agtagcgtcc ccatgagtag gcagaaccgc gtccaagtcc acatcatcca taacgatcat
     2461 gcacggggtg gaatccacac ccagacttgc cagcacctca ttagcgacac gttgcgcagc
     2521 ggccacgtcc ttagccttat ccacgcaatc tagaacgtac tgcctaaccg cgaaatcaga
     2581 ctgaatcagt ttccaatcat cgggcttcac caaagcaaca gcaacgcggg ttgattcgac
     2641 ccgttccggt gcttccagac cggcgagctt gtacagttct tcttccattt cacgacgtac
     2701 atcagcgtct atgtaatcaa tgcccaaagc acgcttagcc ccacgtgacc aggacgaacg
     2761 caggttttta gaaccaacct catactcacg ccaccgagcc accaaaacag cgtccatatc
     2821 ctcgccggcg tcgctttgat cggccaacat atccaacatc tgaaacggcg tgtacgaccc
     2881 cttagacgcg gttttagtag cggagccagt cagttcctga gacatgccct tagcgaggta
     2941 ggttgccatt ttcgcagcgt ctccacccca ggtagacacc tgatcaagtt tgaccccgtg
     3001 ctcacgcagt ggcgcgtcca taccggcctt aaccacacca gcagaccagc gggaaaacat
     3061 ggaatcctca aacgccttga gttcatcgtc agacagtgga cgatccaaga acaacagcat
     3121 gttgcggtgc aagtgccaac cgttcgccca agagtctgtg acctcatagt cactataggt
     3181 gtgctccacc ccgtaccgtg cacgttcttt cttccactga gatgttttca ccatcgaaga
     3241 gtacgcagtc ttaatacccg cttcaacctg cgcaaatgac tgtgagcggt tgtgtcgaac
     3301 agtgcccaca aacatcatga gcgcgccacc cgccgccaag tgattcttag tagcaatagc
     3361 cagctcaatg cggcgttcgc ccatgacttc caattcagcc agaggtgacc cccagcgaga
     3421 gtgagagttt tgcagaccct caaactgcga agcaccgtta gacgaccagg acaccgcaac
     3481 agcttcgtcc ctgcgccacc tatggcaccc cgccagagcc ttactattgg tgatcttgta
     3541 catgacgttt tgcctacgcc acgccctagc gcgagtgacc ttagaaccct cattgacctg
     3601 cggttcctta gaggtgttca cttctatttc agtgttacct agacccgatg ttgtgcgggg
     3661 ttgcgcagtg cgagtttgtg cgggtgttgt gcccgttgtc ttagctagtg ctatggttgt
     3721 caattgaaac cccttcgggt tatgtggccc ccgtgcatat gagttggtag ctcgcacggg
     3781 ggtttgtctt gtctagggac tattaatttt tagtggtgtt tggtggccgc ctagcttggc
     3841 tatgcgtgcc agcttacccg tactcaatgt taaagatttg catcgacatg ggagggttac
     3901 gtgtccgata cctagggggg gtatccgcga ctaggtgccc cggtgctcac tgtctgtacc
     3961 ggcggggcaa gccccacacc ccgcatggac agggtggctc cgccccctgc acccccagca
     4021 atctgcatgt acatgtttta cacattagca cgacatgact gcatgtgcat gcactgcatg
     4081 cagactaggt aaatatgagt atgtacgact agtaacagga gcactgcaca taatgaatga
     4141 gttgcaggac aatgtttgct acgcatgcgc atgacatatc gcaggaaagc tactagagtc
     4201 ttaaagcatg gcaaccaagg cacagctaga acagcaacta caagaagctc aacaggcact
     4261 acaggcgcag caagcgcagg cacaagccac catcgaagca ctagaagcgc aggcaaaggc
     4321 taagcccgtc gtggtcaccg cacgcgttcc tttggcacta cgtgaggaca tgaagcgcgc
     4381 aggcatgcag aacggtgaaa acctccaaga gttcatgatc gccgcgttta ccgagcggct
     4441 agaaaagctc accaccaccg acaacgagga aaacaatgtc taacccacta gttctctttg
     4501 cccaccgtga cccggtaaat gacgtgacgt tcgagtgcat tgagcacgcc acctacgaca
     4561 cactttcaca cgctaaagac cagatcaccg cccaaatgca agccctagac gaagaagccg
     4621 ccctactgcc ctaatgggtg tttcatgggt gtttccctag tgtttcatgg tgttttcacc
     4681 taagctaggg aattgcgcga gaagtctcgc aaaaatcagc aacccccgga accacacagt
     4741 tcacgggggt tcttctatgc cagaaatcag aaaggggaac cagtgaacga ccccgaatct
     4801 gtccctcctg ttcagctact gacggggtgg tgcgtaacgg caaaagcacc gccggacatc
     4861 agcgctagcg gagtgtatac tggcttacta tgttggcact gatgagggtg tcagtgaagt
     4921 gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat gtgatacagg
     4981 atatatttcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc
     5041 ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag
     5101 ggaagtgaga gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat
     5161 cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag
     5221 gcgtttcccc tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg
     5281 tcattccgct gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag
     5341 gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc
     5401 ttatccggta actatcgtct tgagtccaac ccggaaagac atgcaaaagc accactggca
     5461 gcagccactg gtaattgatt tagaggagtc ttgaagtcat gcgccggtta aggctaaagg
     5521 acaagttttg gtgactgcgc tcctccaagc cagttacctc ggttcaaaga gttggtagct
     5581 cagagaacct tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga gcaagagatt
     5641 acgcgcagac caaaacgatc tcaagaagat catcttatta atcagataaa atattaacgt
     5701 ttacaatttc ccattcgcca tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     5761 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5821 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5881 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5941 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6001 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6061 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     6121 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat ctgtccctcc
     6181 tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca tcagcgctag
     6241 c
//
