LOCUS       40924_10571        6272 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pCGL0243, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Reyes O, Guyonvarch A, Bonamy C, Salti V, David F, Leblon G.
  TITLE     'Integron'-bearing vectors: a method suitable for stable chromosomal
            integration in highly restrictive corynebacteria
  JOURNAL   Gene 107 (1), 61-68 (1991)
  PUBMED    1660430
REFERENCE   2  (bases 1 to 6272)
  AUTHORS   Ankri S, Reyes O, Leblon G.
  TITLE     Electrotransformation of highly DNA-restrictive corynebacteria with 
            synthetic DNA
  JOURNAL   Plasmid 35 (1), 62-66 (1996)
  PUBMED    8693028
REFERENCE   3  (bases 1 to 6272)
  AUTHORS   Ankri S, Bouvier I, Reyes O, Predali F, Leblon G.
  TITLE     A Brevibacterium linens pRBL1 replicon functional in Corynebacterium
            glutamicum
  JOURNAL   Plasmid 36 (1), 36-41 (1996)
  PUBMED    8938050
REFERENCE   4  (bases 1 to 6272)
  AUTHORS   Reyes O.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-1998) Laboratoire de Biologie Moleculaire des 
            Corynebacteries, Institut de Genetique et Microbiologie, Universite 
            de Paris-Sud, Orsay 91405, France
REFERENCE   5  (bases 1 to 6272)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 6272)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1991"; volume: "107"; issue: "1"; pages: "61-68"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Plasmid"; 
            date: "1996"; volume: "35"; issue: "1"; pages: "62-66"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Plasmid"; 
            date: "1996"; volume: "36"; issue: "1"; pages: "36-41"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Submitted 
            (09-SEP-1998) Laboratoire de Biologie Moleculaire des 
            Corynebacteries, Institut de Genetique et Microbiologie, Universite 
            de Paris-Sud, Orsay 91405, France"
COMMENT     SGRef: number: 5; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     72..88
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(175..966)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    1624..1920
                     /note="partial sequence of pACYC184 Tn9 (cat) gene from
                     GenBank Accession Number X06403; chloramphenicol sensitive;
                     BclI-ScaI fragment"
     misc_feature    1921..4829
                     /note="cryptic plasmid pBL1 (ATCC accession 21086)
                     replication region; similar to Brevibacterium 
                     lactofermentum plasmid in GenBank Accession Number X03987 
                     (ATCC accession 13869)"
     CDS             complement(2369..3652)
                     /codon_start=1
                     /product="replicase"
                     /label=replicase
                     /protein_id="AAD03697.1"
                     /translation="MNTSKEPQVNEGSKVTRARAWRRQNVMYKITNSKALAGCHRWRRD
                     EAVAVSWSSNGASQFEGLQNSHSRWGSPLAELEVMGERRIELAIATKNHLAAGGALMMF
                     VGTVRHNRSQSFAQVEAGIKTAYSSMVKTSQWKKERARYGVEHTYSDYEVTDSWANGWH
                     LHRNMLLFLDRPLSDDELKAFEDSMFSRWSAGVVKAGMDAPLREHGVKLDQVSTWGGDA
                     AKMATYLAKGMSQELTGSATKTASKGSYTPFQMLDMLADQSDAGEDMDAVLVARWREYE
                     VGSKNLRSSWSRGAKRALGIDYIDADVRREMEEELYKLAGLEAPERVESTRVAVALVKP
                     DDWKLIQSDFAVRQYVLDCVDKAKDVAAAQRVANEVLASLGVDSTPCMIVMDDVDLDAV
                     LPTHGDATKRDLNAAVFAGNEQTILRTH"
     rep_origin      complement(5161..5695)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     rep_origin      5763..6191
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gctaaccgtt tttatcaggc tctgggaggc agaataaatg atccgtcgag ctccaccgcg
       61 gtggcggccg ctctagaact agtggatccc ccgggctgca ggcctcgaga tctccatgga
      121 cgcgtgacgt cgacggtatc gataagcttt ttagacatct aaatctaggt actaaaacaa
      181 ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc
      241 aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa
      301 ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac
      361 tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc
      421 ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt
      481 gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa
      541 ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg
      601 aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc
      661 atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc
      721 atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat
      781 gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa
      841 atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt
      901 atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt
      961 agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat
     1021 tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa
     1081 acagcttttt caaagttgtt ttgaaagttg gcgtataaca tagtatcgac ggagccgatt
     1141 ttgaaaccac aattatgata gaatttacaa gctataaggt tattgtcctg ggtttcaagc
     1201 attagtccat gcaagttttt atgctttgcc cattctatag atatattgat aagcgcgctg
     1261 cctatgcctt gccccctgaa atccttacat acggcgatat cttctatata aaagatatat
     1321 tatcttatca gtattgtcaa tatattcaag gcaatctgcc tcctcatcct cttcatcctc
     1381 ttcgtcttgg tagcttttta aatatggcgc ttcatagagt aattctgtaa aggtccaatt
     1441 ctcgttttca tacctcggta taatcttacc tatcacctca aatggttcgc tgggtttatc
     1501 gatgataagc tgtcaaacat gagaattaca acttatatcg tatggggctg acttcaggtg
     1561 ctacatttga agagataaat tgcactgaaa tctagagcgg ccgccaccgc ggtggagctc
     1621 gacggatcag atcatatcgt caattattac ctccacgggg agagcctgag caaactggcc
     1681 tcaggcattt gagaagcaca cggtcacact gcttccggta gtcaataaac cggtaaacca
     1741 gcaatagaca taagcggcta tttaacgacc ctgccctgaa ccgacgaccg ggtcgaattt
     1801 gctttcgaat ttctgccatt catccgctta ttatcactta ttcaggcgta gcaccaggcg
     1861 tttaagggca ccaataactg ccttaaaaaa attacgcccc gccctgccac tcatcgcagt
     1921 aacgtcaaca accacccccg caggcgttaa gttgccccgc caacagaaag gaaaccaaca
     1981 cgaaacaaca acacaaaaag gtttcacaga aaaaagcgta tgcgctaacg tatgccccgc
     2041 agcacggcaa aacgcgcgtt aagccctagc ccacgcgcgc gtaggtatta ctcatgccca
     2101 ctatggtgtg cacactgccc actacggtgt gcaatctatt cacgatgcca cccccagata
     2161 cagtgaagcc ccgccaatcc gaactagatc agatcaacgg ggcaacccat tgtccccagc
     2221 tttgattagg agccaggcac ataacagcat gacagttcca ttcctgatga aatcagccat
     2281 tgtcaacaac aagacccatc atagtttgcc cccgcgacat tgaccataaa ttcatcgcac
     2341 aaaatatcga acggggttta tgccgctttt agtgggtgcg aagaatagtc tgctcattac
     2401 ccgcgaacac cgccgcattc agatcacgct tagtagcgtc cccatgagta ggcagaaccg
     2461 cgtccaagtc cacatcatcc ataacgatca tgcacggggt ggaatccaca cccagacttg
     2521 ccagcacctc attagcgaca cgttgcgcag cggccacgtc cttagcctta tccacgcaat
     2581 ctagaacgta ctgcctaacc gcgaaatcag actgaatcag tttccaatca tcgggcttca
     2641 ccaaagcaac agcaacgcgg gttgattcga cccgttccgg tgcttccaga ccggcgagct
     2701 tgtacagttc ttcttccatt tcacgacgta catcagcgtc tatgtaatca atgcccaaag
     2761 cacgcttagc cccacgtgac caggacgaac gcaggttttt agaaccaacc tcatactcac
     2821 gccaccgagc caccaaaaca gcgtccatat cctcgccggc gtcgctttga tcggccaaca
     2881 tatccaacat ctgaaacggc gtgtacgacc ccttagacgc ggttttagta gcggagccag
     2941 tcagttcctg agacatgccc ttagcgaggt aggttgccat tttcgcagcg tctccacccc
     3001 aggtagacac ctgatcaagt ttgaccccgt gctcacgcag tggcgcgtcc ataccggcct
     3061 taaccacacc agcagaccag cgggaaaaca tggaatcctc aaacgccttg agttcatcgt
     3121 cagacagtgg acgatccaag aacaacagca tgttgcggtg caagtgccaa ccgttcgccc
     3181 aagagtctgt gacctcatag tcactatagg tgtgctccac cccgtaccgt gcacgttctt
     3241 tcttccactg agatgttttc accatcgaag agtacgcagt cttaataccc gcttcaacct
     3301 gcgcaaatga ctgtgagcgg ttgtgtcgaa cagtgcccac aaacatcatg agcgcgccac
     3361 ccgccgccaa gtgattctta gtagcaatag ccagctcaat gcggcgttcg cccatgactt
     3421 ccaattcagc cagaggtgac ccccagcgag agtgagagtt ttgcagaccc tcaaactgcg
     3481 aagcaccgtt agacgaccag gacaccgcaa cagcttcgtc cctgcgccac ctatggcacc
     3541 ccgccagagc cttactattg gtgatcttgt acatgacgtt ttgcctacgc cacgccctag
     3601 cgcgagtgac cttagaaccc tcattgacct gcggttcctt agaggtgttc acttctattt
     3661 cagtgttacc tagacccgat gttgtgcggg gttgcgcagt gcgagtttgt gcgggtgttg
     3721 tgcccgttgt cttagctagt gctatggttg tcaattgaaa ccccttcggg ttatgtggcc
     3781 cccgtgcata tgagttggta gctcgcacgg gggtttgtct tgtctaggga ctattaattt
     3841 ttagtggtgt ttggtggccg cctagcttgg ctatgcgtgc cagcttaccc gtactcaatg
     3901 ttaaagattt gcatcgacat gggagggtta cgtgtccgat acctaggggg ggtatccgcg
     3961 actaggtgcc ccggtgctca ctgtctgtac cggcggggca agccccacac cccgcatgga
     4021 cagggtggct ccgccccctg cacccccagc aatctgcatg tacatgtttt acacattagc
     4081 acgacatgac tgcatgtgca tgcactgcat gcagactagg taaatatgag tatgtacgac
     4141 tagtaacagg agcactgcac ataatgaatg agttgcagga caatgtttgc tacgcatgcg
     4201 catgacatat cgcaggaaag ctactagagt cttaaagcat ggcaaccaag gcacagctag
     4261 aacagcaact acaagaagct caacaggcac tacaggcgca gcaagcgcag gcacaagcca
     4321 ccatcgaagc actagaagcg caggcaaagg ctaagcccgt cgtggtcacc gcacgcgttc
     4381 ctttggcact acgtgaggac atgaagcgcg caggcatgca gaacggtgaa aacctccaag
     4441 agttcatgat cgccgcgttt accgagcggc tagaaaagct caccaccacc gacaacgagg
     4501 aaaacaatgt ctaacccact agttctcttt gcccaccgtg acccggtaaa tgacgtgacg
     4561 ttcgagtgca ttgagcacgc cacctacgac acactttcac acgctaaaga ccagatcacc
     4621 gcccaaatgc aagccctaga cgaagaagcc gccctactgc cctaatgggt gtttcatggg
     4681 tgtttcccta gtgtttcatg gtgttttcac ctaagctagg gaattgcgcg agaagtctcg
     4741 caaaaatcag caacccccgg aaccacacag ttcacggggg ttcttctatg ccagaaatca
     4801 gaaaggggaa ccagtgaacg accccgaatc tgtccctcct gttcagctac tgacggggtg
     4861 gtgcgtaacg gcaaaagcac cgccggacat cagcgctagc ggagtgtata ctggcttact
     4921 atgttggcac tgatgagggt gtcagtgaag tgcttcatgt ggcaggagaa aaaaggctgc
     4981 accggtgcgt cagcagaata tgtgatacag gatatatttc cgcttcctcg ctcactgact
     5041 cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt
     5101 tcctggaaga tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt
     5161 tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg
     5221 gcgaaacccg acaggactat aaagatacca ggcgtttccc ctggcggctc cctcgtgcgc
     5281 tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct
     5341 cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca
     5401 cgaacccccc gttcagtccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5461 cccggaaaga catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt
     5521 cttgaagtca tgcgccggtt aaggctaaag gacaagtttt ggtgactgcg ctcctccaag
     5581 ccagttacct cggttcaaag agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa
     5641 ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga
     5701 tcatcttatt aatcagataa aatattaacg tttacaattt cccattcgcc atggcgaatg
     5761 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     5821 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     5881 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     5941 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     6001 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     6061 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     6121 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     6181 taacgcgaat tttaacaaaa tctgtccctc ctgttcagct actgacgggg tggtgcgtaa
     6241 cggcaaaagc accgccggac atcagcgcta gc
//
