LOCUS       62056_6265        5982 bp DNA     circular SYN 02-MAY-2020
DEFINITION  Cloning vector pCG403, complete sequence.
ACCESSION   MN744967
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Gao C, Fernandez VI, Lee KS, Fenizia S, Pohnert G, Seymour JR, Raina
            J-B., Stocker R.
  TITLE     Single-cell bacterial transcription measurements reveal the 
            importance of dimethylsulfoniopropionate (DMSP) hotspots in ocean 
            sulfur cycling
  JOURNAL   Nat Commun 11 (1) (2020) In press
REFERENCE   2  (bases 1 to 5982)
  AUTHORS   Gao C.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-NOV-2019) Biological Engineering, Massachusetts 
            Institute of Technology, 15 Vassar Street, Cambridge, MA 20139, USA
REFERENCE   3  (bases 1 to 5982)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            11 (1) (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-NOV-2019) Biological Engineering, Massachusetts Institute of 
            Technology, 15 Vassar Street, Cambridge, MA 20139, USA"
FEATURES             Location/Qualifiers
     source          1..5982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1023..1792
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1793..2452
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     regulatory      2887..3386
                     /label=putative PdddW
                     /note="putative PdddW"
                     /regulatory_class="promoter"
     CDS             3387..4094
                     /label=mTFP1
                     /note="monomeric teal (cyan) fluorescent protein (Ai et
                     al., 2006)"
     regulatory      4119..4241
                     /label=TR2-17
                     /note="TR2-17"
                     /regulatory_class="terminator"
     regulatory      4242..4318
                     /label=TL17
                     /note="TL17"
                     /regulatory_class="terminator"
     regulatory      4331..4383
                     /label=BS7
                     /note="BS7"
                     /regulatory_class="terminator"
     terminator      complement(4408..4435)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(4451..4522)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(4734..5525)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
     2641 ggcagaataa atgatgtcga caaaaataat gagaatcaat agaacttccg agaagttcag
     2701 ccgctaataa tcgccctgct ccattgtgcg ccgcaataaa agtaccggca ttacgggtgc
     2761 attggcgcgc caaatgcggc catctgtggg caacctgtgc ggtaagaccc aaacttagtg
     2821 taataggtat cctatgatta ttttttcatt tgatgccaaa aagacaatga acccccgctc
     2881 gagcgggggt cgcctcacca tactcctgac ttatgatccg gatggtcaca tcctcgaaag
     2941 cagcctccag ttcccccagc tgcggaatca gccaaaaggc gacaaagccc gacggcgcag
     3001 tcacggtcac caccttgccc tgcgccgctt gccggatttg ggcaaccgcc gcctcgatct
     3061 ggccgaaccc accttgcaaa gcccgcagca ggtcccggcc tgactgggtc atacgcaccg
     3121 gtttgtgact gcgatcgaac agcggactgc ccaaatccgc ctccagcatc gcgatccggc
     3181 gcgagaccgc gggttgcgac acgttcagct ctgccgctgc cgcggtcagc gtgccgcgac
     3241 gcgcggccgc gtagaatgca gtcaagccgg agaagcttgg caaacgctcc atatcataac
     3301 ctcatcgtat aatctgatgc agttttacgc gattgcgccc cccctgcaag agcgactaac
     3361 tgaccccaag cacaaaggag cccatcatgg tgagcaaggg cgaggagacc acaatgggcg
     3421 taatcaagcc cgacatgaag atcaagctga agatggaggg caacgtgaat ggccacgcct
     3481 tcgtgatcga gggcgagggc gagggcaagc cctacgacgg caccaacacc atcaacctgg
     3541 aggtgaagga gggagccccc ctgcccttct cctacgacat tctgaccacc gcgttcgcct
     3601 acggcaacag ggccttcacc aagtaccccg acgacatccc caactacttc aagcagtcct
     3661 tccccgaggg ctactcttgg gagcgcacca tgaccttcga ggacaagggc atcgtgaagg
     3721 tgaagtccga catctccatg gaggaggact ccttcatcta cgagatacac ctcaagggcg
     3781 agaacttccc ccccaacggc cccgtgatgc agaagaagac caccggctgg gacgcctcca
     3841 ccgagaggat gtacgtgcgc gacggcgtgc tgaagggcga cgtcaagcac aagctgctgc
     3901 tggagggcgg cggccaccac cgcgttgact tcaagaccat ctacagggcc aagaaggcgg
     3961 tgaagctgcc cgactatcac tttgtggacc accgcatcga gatcctgaac cacgacaagg
     4021 actacaacaa ggtgaccgtt tacgagagcg ccgtggcccg caactccacc gacggcatgg
     4081 acgagctgta caagtaataa taatgcaggt cgtctcggat cgagaaggac acggttaata
     4141 ctaggcctgc tggctggtaa tcgccagcag gcctttttat ttgggggaga gggaagtcat
     4201 gaaaaaacta acctttgaaa ttcgatctcc accacatcag ctctgaagca acgtaaaaaa
     4261 acccgccccg gcgggttttt ttatacccgt agtatcccca cttatctaca atagctgtcc
     4321 ttaattaagg ttgaataaat aaaaacagcc gttgccagaa agaggcacgg ctgtttttat
     4381 tttctagtga gaccgggagc agttaaacgc agaaaggccc acccgaaggt gagccagtgt
     4441 gactctagta gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg
     4501 actgagcctt tcgttttatt tgatgcctgg tcgccttgcg tataatattt gcccatgggg
     4561 gtgggcgaag aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg
     4621 gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga
     4681 tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac
     4741 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
     4801 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
     4861 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag
     4921 cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc
     4981 tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
     5041 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc
     5101 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
     5161 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
     5221 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
     5281 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
     5341 tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
     5401 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
     5461 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
     5521 atcatgcgaa acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag
     5581 atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag
     5641 ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat
     5701 cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt ttcccttgtc
     5761 cagatagccc agtagctgac attcatccca ggtggcactt ttcggggaaa tgtgcgcgcc
     5821 cgcgttcctg ctggcgctgg gcctgtttct ggcgctggac ttcccgctgt tccgtcagca
     5881 gcttttcgcc cacggccttg atgatcgcgg cggccttggc ctgcatatcc cgattcaacg
     5941 gccccagggc gtccagaacg ggcttcaggc gctcccgaag gt
//
