LOCUS       62056_6245        5281 bp DNA     circular SYN 02-MAY-2020
DEFINITION  Cloning vector pCG302, complete sequence.
ACCESSION   MN744963
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Gao C, Fernandez VI, Lee KS, Fenizia S, Pohnert G, Seymour JR, Raina
            J-B., Stocker R.
  TITLE     Single-cell bacterial transcription measurements reveal the 
            importance of dimethylsulfoniopropionate (DMSP) hotspots in ocean 
            sulfur cycling
  JOURNAL   Nat Commun 11 (1) (2020) In press
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Gao C.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-NOV-2019) Biological Engineering, Massachusetts 
            Institute of Technology, 15 Vassar Street, Cambridge, MA 20139, USA
REFERENCE   3  (bases 1 to 5281)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            11 (1) (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-NOV-2019) Biological Engineering, Massachusetts Institute of 
            Technology, 15 Vassar Street, Cambridge, MA 20139, USA"
FEATURES             Location/Qualifiers
     source          1..5281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1023..1792
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1793..2452
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     regulatory      2656..2877
                     /label=putative PdmdA
                     /note="putative PdmdA"
                     /regulatory_class="promoter"
     CDS             2878..3585
                     /label=mTFP1
                     /note="monomeric teal (cyan) fluorescent protein (Ai et
                     al., 2006)"
     regulatory      3610..3783
                     /label=TSAL2
                     /note="TSAL2"
                     /regulatory_class="terminator"
     regulatory      complement(3784..3829)
                     /label=RNAI
                     /note="RNAI"
                     /regulatory_class="terminator"
     CDS             complement(4033..4824)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
     2641 ggcagaataa atgatggctg tggtcctgac gccgggtcgc accggcgcgg cgccggcaag
     2701 aattcatttg tctgacattt aaaagtctga catttaagac cctatgacgt gagtcgtccg
     2761 agggcaagag ccgcaatgac ggtcgcagcg aatgccggaa gggcgattgc accgccgcat
     2821 caccgccggg cctctcgttc ggcccgcata ccataacgct cgtctggaga tccgatcatg
     2881 gtgagcaagg gcgaggagac cacaatgggc gtaatcaagc ccgacatgaa gatcaagctg
     2941 aagatggagg gcaacgtgaa tggccacgcc ttcgtgatcg agggcgaggg cgagggcaag
     3001 ccctacgacg gcaccaacac catcaacctg gaggtgaagg agggagcccc cctgcccttc
     3061 tcctacgaca ttctgaccac cgcgttcgcc tacggcaaca gggccttcac caagtacccc
     3121 gacgacatcc ccaactactt caagcagtcc ttccccgagg gctactcttg ggagcgcacc
     3181 atgaccttcg aggacaaggg catcgtgaag gtgaagtccg acatctccat ggaggaggac
     3241 tccttcatct acgagataca cctcaagggc gagaacttcc cccccaacgg ccccgtgatg
     3301 cagaagaaga ccaccggctg ggacgcctcc accgagagga tgtacgtgcg cgacggcgtg
     3361 ctgaagggcg acgtcaagca caagctgctg ctggagggcg gcggccacca ccgcgttgac
     3421 ttcaagacca tctacagggc caagaaggcg gtgaagctgc ccgactatca ctttgtggac
     3481 caccgcatcg agatcctgaa ccacgacaag gactacaaca aggtgaccgt ttacgagagc
     3541 gccgtggccc gcaactccac cgacggcatg gacgagctgt acaagtaata ataatcatcg
     3601 cgaagacttg atcggtgccg ggattgtgct gcgacgcaat gcgctacgta gctataagcc
     3661 aaaagccttg tatgcttctt tgccggatgg tggcggctac gacaggtaaa atttctcatt
     3721 aaaaaaccgg gcattgcccg gtttttttac gcttattgat gacgcttacg aagattgtcg
     3781 atccaaacaa aaaaaccacc gctaccaacg gtggtttgtt tgccggatct cgccttgcgt
     3841 ataatatttg cccatggggg tgggcgaaga actccagcat gagatccccg cgctggagga
     3901 tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg
     3961 tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca
     4021 gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg
     4081 agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc
     4141 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca
     4201 gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc
     4261 atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc
     4321 ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc
     4381 catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc
     4441 cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg
     4501 agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct
     4561 tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca
     4621 cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac
     4681 aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat
     4741 tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc
     4801 gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcagatct
     4861 tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg
     4921 cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctgtccataa
     4981 aaccgcccag tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc
     5041 gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatcccag gtggcacttt
     5101 tcggggaaat gtgcgcgccc gcgttcctgc tggcgctggg cctgtttctg gcgctggact
     5161 tcccgctgtt ccgtcagcag cttttcgccc acggccttga tgatcgcggc ggccttggcc
     5221 tgcatatccc gattcaacgg ccccagggcg tccagaacgg gcttcaggcg ctcccgaagg
     5281 t
//
