LOCUS       V008549                 5508 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008549
VERSION     V008549
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Wang G, Jones H, Doherty A, Yu X, Ma Y, Sun Y, Du W, Wang C, Xia L.
  TITLE     Generation of marker- and backbone-free transgenic wheat plants via
            Agrobacterium-mediated transformation based on the pCLEAN dual
            binary vector system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   Wang G, Jones H, Doherty A, Yu X, Ma Y, Sun Y, Du W, Wang C, Xia L.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2015) Institute of Crop Sciences, Chinese Academy
            of Agricultural Sciences, 12 Zhongguancun Street, Beijing, Beijing
            100081, China
REFERENCE   3  (bases 1 to 5508)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5508)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (17-JUN-2015) Institute of Crop Sciences, Chinese Academy of
            Agricultural Sciences, 12 Zhongguancun Street, Beijing, Beijing
            100081, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(61..649)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(823..1635)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1926..2361
                     /label="pSa ori"
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    2508..2532
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    2533..2558
                     /note="spacer sequence (Thole et al., (2007) Plant Physiol
                     145:1211-1219)"
     misc_feature    2559..2583
                     /note="T-DNA left border No. 2 sequence (Thole et al.,
                     (2007) Plant Physiol 145:1211-1219)"
     protein_bind    2599..2723
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2767..3069)
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(3414..4070)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(4124..4154)
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(4179..4303)
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    4351..4375
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    4376..4399
                     /note="right border overdrive sequence (Thole et al.,
                     (2007) Plant Physiol 145:1211-1219)"
     CDS             4609..5409
                     /gene="virG"
                     /label="Regulatory protein VirG"
                     /note="Regulatory protein VirG from Agrobacterium
                     tumefaciens (strain 15955). Accession#: P62722"
ORIGIN
        1 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
       61 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      481 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      541 cttcggaaga agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      601 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      661 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      721 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      781 atcaatctaa agtatatatg tgtaacattg gtctagtgat tagaaaaact catcgagcat
      841 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      901 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      961 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     1021 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     1081 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     1141 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
     1201 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     1261 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     1321 tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     1381 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     1441 aacaacattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     1501 cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     1561 cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg
     1621 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
     1681 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
     1741 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
     1801 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa
     1861 caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc
     1921 gatccccatc caacagcccg ccgtcgagcg ggctttttta tccccggaag cctgtggata
     1981 gagggtagtt atccacgtga aaccgctaat gccccgcaaa gccttgattc acggggcttt
     2041 ccggcccgct ccaaaaacta tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa
     2101 tcggagtacg tgaaatcgct aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga
     2161 gaacgctaat agccctttca gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt
     2221 tacagcaagt agtatgttca attagctttt caattatgaa tatatatatc aattattggt
     2281 cgcccttggc ttgtggacaa tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag
     2341 cggttgccca ccgtcgagcg cctttgccca caacccggcg gccggccgca acagatcgtt
     2401 ttataaattt ttttttttga aaaagaaaaa gcccgaaagg cggcaacctc tcgggcttct
     2461 ggatttccga tccccggaat tagatccgtt taaactacgt aagatcttgg caggatatat
     2521 tgtggtgtaa acgttcctgc ggcggtcgag atggatcttg gcaggatata ttgtggtgta
     2581 aacgttcctg cggccatcac cactttgtac aagaaagctg aacgagaaac gtaaaatgat
     2641 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa
     2701 acacaacata tccagtcact atggtcgacc tgcagactgg ctgtgtataa gggagcctga
     2761 catttatatt ccccagaaca tcaggttaat ggcgtttttg atgtcatttt cgcggtggct
     2821 gagatcagcc acttcttccc cgataacgga gaccggcaca ctggccatat cggtggtcat
     2881 catgcgccag ctttcatccc cgatatgcac caccgggtaa agttcacggg agactttatc
     2941 tgacagcaga cgtgcactgg ccagggggat caccatccgt cgcccgggcg tgtcaataat
     3001 atcactctgt acatccacaa acagacgata acggctctct cttttatagg tgtaaacctt
     3061 aaactgcatt tcaccagccc ctgttctcgt cagcaaaaga gccgttcatt tcaataaacc
     3121 gggcgacctc agccatccct tcctgatttt ccgctttcca gcgttcggca cgcagacgac
     3181 gggcttcatt ctgcatggtt gtgcttacca gaccggagat attgacatca tatatgcctt
     3241 gagcaactga tagctgtcgc tgtcaactgt cactgtaata cgctgcttca tagcatacct
     3301 ctttttgaca tacttcgggt atacatatca gtatatattc ttataccgca aaaatcagcg
     3361 cgcaaatacg catactgtta tctggctttt agtaagccgg atccacgcgt ttacgccccg
     3421 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
     3481 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     3541 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     3601 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac
     3661 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     3721 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     3781 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     3841 attgccatac ggaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     3901 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
     3961 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
     4021 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
     4081 ttagctcctg aaaatctcga cggatcctaa ctcaaaatcc acacattata cgagccggaa
     4141 gcataaagtg taaagcctgg ggtgcctaat gcggccgcca tagtgactgg atatgttgtg
     4201 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
     4261 tatcatttta cgtttctcgt tcagcttttt tgtacaaact tgtgatggcc gcttaattaa
     4321 ggccgtacgg gccctcgagt cgacgttcct tgacaggata tattggcggg taaactaagt
     4381 cgctgtatgt gtttgtttga gatcctctag ggcatgcagg ctcgcggcgg acgcacgacg
     4441 ccggggcgag accataggcg atctcctaaa tcaatagtag ctgtaacctc gaagcgtttc
     4501 acttgtaaca acgattgaga atttttgtca taaaattgaa atacttggtt cgcatttttg
     4561 tcatccgcgg tcagccgcaa ttctgacgaa ctgcccattt agctggagat gattgtacat
     4621 ccttcacgtg aaaatttctc aagcgctgtg aacaagggtt cagattttag attgaaaggt
     4681 gagccgttga aacacgttct tcttgtcgat gacgacgtcg ctatgcggca tcttattatt
     4741 gaatacctta cgatccacgc cttcaaagtg accgcggtag ccgacagcac ccagttcaca
     4801 agagtactct cttccgcgac ggtcgatgtc gtggttgttg atctaaattt aggtcgtgaa
     4861 gatgggctcg agatcgttcg taatctggcg gcaaagtctg atattccaat cataattatc
     4921 agtggcgacc gccttgagga gacggataaa gttgttgcac tcgagctagg agcaagtgat
     4981 tttatcgcta agccgttcag tatcagagag tttctagcac gcattcgggt tgccttgcgc
     5041 gtgcgcccca acgttgtccg ctccaaagac cgacggtctt tttgttttac tgactggaca
     5101 cttaatctca ggcaacgtcg cttgatgtcc gaagctggcg gtgaggtgaa acttacggca
     5161 ggtgagttca atcttctcct cgcgttttta gagaaacccc gcgacgttct atcgcgcgag
     5221 caacttctca ttgccagtcg agtacgcgac gaggaggttt atgacaggag tatagatgtt
     5281 ctcattttga ggctgcgccg caaacttgag gcggatccgt caagccctca actgataaaa
     5341 acagcaagag gtgccggtta tttctttgac gcggacgtgc aggtttcgca cggggggacg
     5401 atggcagcct gagccaattg catttgcctc ttaattatct ggctcaaagg gtgactgagg
     5461 agtaagcgat gtgcccatca cactgcgcat gcaagctgat ctggatct
//
