LOCUS       40924_10526        9113 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Promoter-probe vector pCG1408, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 5465 to 8413)
  AUTHORS   David M, Bartz SL, Weaver JD, Kelley JL.
  TITLE     Isolation and Characterization of Promoter Sequences from 
            Clavibacter xyli subsp. cynodontis
  JOURNAL   Unpublished
REFERENCE   2  (bases 5465 to 8413)
  AUTHORS   Taylor J, Stearman RS, Uratani BB.
  TITLE     Development of a native plasmid as a cloning vector in Clavibacter 
            xyli subsp. cynodontis
  JOURNAL   Plasmid 29 (3), 241-244 (1993)
  PUBMED    7689234
REFERENCE   3  (bases 1 to 9113)
  AUTHORS   David M.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-FEB-1995) Michael David, Crop Genetics, 10150 Old 
            Columbia Rd, Columbia, MD 21046, USA, 21046
REFERENCE   4  (bases 1 to 9113)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 9113)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Plasmid"; 
            date: "1993"; volume: "29"; issue: "3"; pages: "241-244"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (17-FEB-1995) Michael David, Crop Genetics, 10150 Old Columbia Rd, 
            Columbia, MD 21046, USA, 21046"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             88..744
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1182..1268
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1360..1387
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      1680..1766
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     regulatory      1684..1726
                     /label=T1
                     /note="T1"
                     /regulatory_class="terminator"
     regulatory      1858..1885
                     /label=T2
                     /note="T2"
                     /regulatory_class="terminator"
     terminator      1858..1885
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2597..2701
                     /label=AmpR promoter
     CDS             2702..3559
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3733..4321
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(4507..4647)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(4752..4940)
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     rep_origin      5465..8413
                     /label=Clavibacter origin of replication
                     /note="Clavibacter origin of replication"
                     /citation=[1]
                     /citation=[2]
     promoter        complement(8725..8829)
                     /label=AmpR promoter
     terminator      complement(8947..8990)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 cccggggatc cgtcgacctg cagccaagct tgagtaggac aaatccgccg agcttcgacg
       61 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      121 gatatatccc aatcgcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      181 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      241 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      301 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      361 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      421 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      481 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
      541 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
      601 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
      661 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
      721 tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca gttattggtg cccttaaacg
      781 cctggtgcta cgcctgaata agtgataata agcggatgaa tggcagaaat tcgtcgaggc
      841 ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa
      901 ctgtgaatgc gcaaaccaac ccttggcaga acatatccat cgcgtccgcc atctccagca
      961 gccgcacgcg gcgcatctcg gctgttttgg cggatgagag aagattttca gcctgataca
     1021 gattaaatca gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc
     1081 ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag
     1141 tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc
     1201 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
     1261 ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg
     1321 caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg
     1381 gcctttttgc gtttctacaa actcttcctg tcgtcatatc tacaagccat ccccccacag
     1441 atacggtaaa ctagcctcgt ttttgcatca ggaaagcagc tgttttggcg gatgagagaa
     1501 gattttcagc ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt
     1561 gcctggcggc agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg
     1621 ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc
     1681 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     1741 tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac
     1801 ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga
     1861 aggccatcct gacggatggc ctttttgcgt ttctacaaac tcttcctgtc gtcatatcta
     1921 caagccatcc ccccacagat acggtaaact agcctcgttt ttgcatcagg aaagcagtcg
     1981 ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg
     2041 ctaggctggc ggggttgcct tactggttag cagaatgaat caccgatacg cgagcgaacg
     2101 tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat ggtcttcggt
     2161 ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg
     2221 atctgcatcg caggatgctg ctggctaccc tgtggaacac ctacatctgt attaacgaag
     2281 cgctggcatt gaccctgagt gatttttctc tggtcccgcc gcatccatac cgccagttgt
     2341 ttaccctcac aacgttccag taaccgggca tgttcatcat cagtaacccg tatcgtgagc
     2401 atcctctctc gtttcatcgg tatcattacc cccatgaaca gaaatccccc ttacacggag
     2461 gcatcagtga ccaaacagga aaaaaccgcc caattcttga agacgaaagg gcctcgtgat
     2521 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     2581 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     2641 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     2701 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     2761 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     2821 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     2881 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     2941 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     3001 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     3061 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     3121 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     3181 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     3241 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     3301 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     3361 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     3421 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     3481 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     3541 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     3601 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     3661 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     3721 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3781 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3841 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     3901 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3961 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4021 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4081 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4141 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4201 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4261 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4321 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4381 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     4441 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     4501 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     4561 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
     4621 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
     4681 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     4741 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc
     4801 tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg
     4861 ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg
     4921 gttttttcct gtttggtcac ttgatgcctc cgtgtaaggg ggaatttctg ttcatggggg
     4981 taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg
     5041 cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga
     5101 gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg
     5161 gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc
     5221 gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg
     5281 cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct
     5341 aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc
     5401 gcacccgtgg ccaggaccca acgactgccc gagatcgatg gcgggcccgg cgagctccca
     5461 tgggagcatc accagcgacg gcccggcccc ttcttcacac gacgagacga agacaaaaca
     5521 agtcccgaaa ctactcaggt ggtgcaacgg ccgcttgaac ccacccaatt gaggactcgg
     5581 cagggtgtta cttgtcgctg gtcgattcgt tcgggtgacg aggtcgcgac atacccaatg
     5641 ccgtcgatgc tattatcagg gggtgattat ggtggcctcg gatctgaagc atcttggctt
     5701 gtctcggcag cctgccgtgt gccttaaggg atgtccgggg ttgtcgggtt gcatgccaaa
     5761 ggcgcacttc ccggactttc gatcatcatg cgccgacgag atgcgttctg ggcggccaac
     5821 tcgacacgcc cgtcgcttag cgttaacggg ccatttaacc cgcaatactc gccctatgac
     5881 cacgggaaac agtgccggga ttaagcggaa tcgccgccct aaaggcgtca aagtagttga
     5941 gagcaaggtc tggctcgaag aagcgaccga tcgacgactc agagaggcgg gcctggcgtc
     6001 cggctcgctt tcgctttcgc tctacttaga gcgcctcgtc tcgcaactcg aagccgagaa
     6061 tggcggactc cccgtactgt cccccacact cggcggcgtg gaggtgacgg ccacgacagc
     6121 ggcttaacca gaaagggccc atcacctagg tgacgggcct ctcccaaatt ctggagctca
     6181 aacccttcaa atcttggcgg gttcgttggt tcgagcttca tctcgaaagt taagtccccc
     6241 tggaagggag gcttttccgt actctctgag aggagttggc aacatcgtaa cccagatttc
     6301 aggttcttgc acgtgcgcac gcggtccgcg tgtcggcgtg ctggccgcag accgctccgt
     6361 cgcccgcctt gtcgacgtcg cgccgcagcg gtctctctct ctccccccca ttgcttcccc
     6421 gtccggccct gtcacaacgt cgtgccaggc cgctgacggg tcgcgtcctg cacgcccgat
     6481 cgacctggtc gacggcgcgt gcgtccagct cctcacggta aggaggacgg tccacctgat
     6541 ctagcgcaga gaccgcggcc ggcgcctggc gccggccctc acccgtaccg gtcggacccg
     6601 catccagggt ccgatgcatc gcatcgaaag aacgccgagt cacagtcggc gatctttgtg
     6661 tatccgaaaa cagggccgag cacataccgg cagctttgct gtacccattt tcaggccgtg
     6721 gaggtcccca gagccatgag aggcaccagc gcgaagaaac gtcgccggca gctggcggcc
     6781 gcgtccgcgt cgcggcggcc gcgcgagctg ccagccacag ctgtgcgctc ggccggcgag
     6841 ctgttcgatc agctgcagca gttcgctcag gccgcgcccc cagcgccgga gggccgggtg
     6901 cgggagacga gctcctggtc gatcccgatc gaccccggcg cgtgggcggc cgtgccgtgc
     6961 tggcgatcgc gccaacactg gctggacacc ctcctcgacc tccaaaccgc gcatgacccc
     7021 cgctggctgg aagcccggac ccggtggaag atggccgccc ggaccgtgtt cgccatcgcc
     7081 tacgtcctcg cccacgccgc cgaccgtgaa accggccgac acgtcaccgt ctcctacgac
     7141 accgtccgcg cccgcgtcct ggccaacacg accgccgcgc ccggccggcc gatccgcctc
     7201 accagcaaca gcgccatcgc caaggccatc aaaatgctcg aacacatcgg cctcatccgc
     7261 ttcatggcca ccggccgcaa cttcctctcc cgcgaggaga aagccgaggc gttccacacc
     7321 cacggcggcc agcaggccgc cgctgcgaac gttttcgcct gcaccctccc ccagcacctc
     7381 gccgcaccgg cggacagcgc tcagacgacc gctgacggcg ctgcgccggc gcccgctcag
     7441 gctgtggaaa actctgggcc caccagtccg atttccgccc tatccccgct acgctctcca
     7501 aaccttccat cttccgaaaa cctacatcac ccatcccaag ggggattgga gcgccctacg
     7561 ggcgcacaac ctggacagaa gagggttttt cgtgaggatg catcagctaa cgtcccagag
     7621 tcaggcagga gcctgcatcg acaggtcctg ctcgcccgcc tagaccagtg gtcgagcgga
     7681 aaacccctcg ttcttcatcc tcacgctgcc aggcccgcag atggcgacct accgcgcgga
     7741 agcctgagcg ccgagaaacc cggcaagaag gtcccgtggc ggcgcaacca catcggccgc
     7801 gtcgagaaag ccctcgaagc cgccggcgtc gacctcgacc actggcagtg gtggcagatc
     7861 gtcgaacagc tcgaacgcgc ctacgccacc aacaaactca ccgccgccca gcgcatcgcc
     7921 tcctccggtg accccctcgc ctacttcacc tggatgatcc gcaacaccat cgcccccggc
     7981 gacgaccccc gcaccatcgt cccctacacc ccctggaacc ccgcctatca accggccact
     8041 cccccggccg accaggcgcc ggcaccctcc cccgcgccaa ccctggccga gcgccaagcg
     8101 atcgacacca ccatccagac catgcgcgac gacgccgcgg ctgcgcgcga cgcccgccgt
     8161 caccagctgc acatcgaccg acgtcagctc accgacgtcc tcctcggcga aggcaaacac
     8221 cccagcgact tccccaccat cgtccgcgac ctccacaacg aaatccaaac ccttcacgaa
     8281 cgcctcacca accacggctg gaccctcgac cgcgacgaac tccaccgcgg cgagatccgc
     8341 tggaccaacg aaaccacccg caccacccgg ccacagccct cgggcgccga ggccgtcacg
     8401 agcatctgaa tttgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8461 ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt
     8521 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     8581 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     8641 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8701 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8761 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8821 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     8881 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattcccagg
     8941 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     9001 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag
     9061 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggga att
//
