LOCUS       V008554                 7265 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008554
VERSION     V008554
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7265)
  AUTHORS   Bisicchia P, Botella E, Devine KM.
  TITLE     Suite of novel vectors for ectopic insertion of GFP, CFP and IYFP
            transcriptional fusions in single copy at the amyE and bglS loci in
            Bacillus subtilis
  JOURNAL   Plasmid 64 (3), 143-149 (2010)
   PUBMED   20600285
REFERENCE   2  (bases 1 to 7265)
  AUTHORS   Bisicchia P.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2010) Trinity College Dublin, 13 Meades Terrace,
            Dublin 2, Ireland
REFERENCE   3  (bases 1 to 7265)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7265)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid";
            date: "2010"; volume: "64"; issue: "3"; pages: "143-149"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-MAY-2010) Trinity College Dublin, 13 Meades Terrace, Dublin 2,
            Ireland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     115..651
                     /label="amyE front"
                     /note="amyE front"
     CDS             complement(661..1377)
                     /label="mECFP"
                     /note="enhanced CFP with monomerizing A206K mutation
                     (Zacharias et al., 2002)"
     misc_feature    complement(1378..1413)
                     /label="LIC site"
                     /note="LIC site"
     CDS             1566..2213
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_recomb     2587..3623
                     /label="amyE back"
                     /note="amyE back"
     CDS             4817..5005
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    5110..5250
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5436..6024)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6198..7055)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7056..7160)
                     /label="AmpR promoter"
ORIGIN
        1 aacaaaattc tccagtcttc acatcggttt gaaaggagga agcggaagaa tgaagtaaga
       61 gggatttttg actccgaagt aagtcttcaa aaaatcaaat aaggagtgtc aagaatgttt
      121 gcaaaacgat tcaaaacctc tttactgccg ttattcgctg gatttttatt gctgtttcat
      181 ttggttctgg caggaccggc ggctgcgagt gctgaaacgg cgaacaaatc gaatgagctt
      241 acagcaccgt cgatcaaaag cggaaccatt cttcatgcat ggaattggtc gttcaatacg
      301 ttaaaacaca atatgaagga tattcatgat gcaggatata cagccattca gacatctccg
      361 attaaccaag taaaggaagg gaatcaagga gataaaagca tgtcgaactg gtactggctg
      421 tatcagccga catcgtatca aattggcaac cgttacttag gtactgaaca agaatttaaa
      481 gaaatgtgtg cagccgctga agaatatggc ataaaggtca ttgttgacgc ggtcatcaat
      541 cataccacca gtgattatgc cgcgatttcc aatgaggtta agagtattcc aaactggaca
      601 catggaaaca cacaaattaa aaactggtct gatcgaaata gtacataatg ggaattctta
      661 cttataaagt tcgtccatgc caagtgtaat gcccgcagct gtgacaaact ctaacagaac
      721 catgtgatcg cgcttttcgt tagggtcttt tgacagctta gattgcgtgc ttaagtaatg
      781 gttgtcagga agaagaaccg gtccatctcc aatcggtgta ttctgctggt aatgatctgc
      841 taattgaacg cttccgtcct caatgttatg gcggattttg aaattagcct tgattccgtt
      901 tttttgctta tctgctgtga tataaacatt gtgagagatg taattatatt ccagtttgtg
      961 tcctaagatg ttgccatcct ccttaaagtc aattcctttc agttcgattc tattgacaag
     1021 cgtgtcgcct tcaaatttga cttccgcgcg tgttttataa tttccgtcgt ccttgaaaaa
     1081 aatcgtgcgt tcttgcacat agccttccgg catggctgat ttgaagaaat cgtgctgttt
     1141 catgtgatcc gggtatctgc tgaagcactg tactccccac gtaagtgtcg tgactaatgt
     1201 aggccatggg acaggtaact ttcctgttgt gcagatgaac ttaagcgtca gtttgccgta
     1261 cgtagcatct ccttctccct cgcctgagac agaaaattta tggccattga catcgccatc
     1321 taattcgacc agaatcggca ctactcccgt aaacagttct tcgccttttg aaaccatagt
     1381 agttcctcct tcccgggaaa gcccgcggta aaagaattcg aaaattggat aaagtgggat
     1441 atttttaaaa tatatattta tgttacagta atattgactt ttaaaaaagg attgattcta
     1501 atgaagaaag cagacaagta agcctcctaa attcacttta gataaaaatt taggaggcat
     1561 atcaaatgaa ctttaataaa attgatttag acaattggaa gagaaaagag atatttaatc
     1621 attatttgaa ccaacaaacg acttttagta taaccacaga aattgatatt agtgttttat
     1681 accgaaacat aaaacaagaa ggatataaat tttaccctgc atttattttc ttagtgacaa
     1741 gggtgataaa ctcaaataca gcttttagaa ctggttacaa tagcgacgga gagttaggtt
     1801 attgggataa gttagagcca ctttatacaa tttttgatgg tgtatctaaa acattctctg
     1861 gtatttggac tcctgtaaag aatgacttca aagagtttta tgatttatac ctttctgatg
     1921 tagagaaata taatggttcg gggaaattgt ttcccaaaac acctatacct gaaaatgctt
     1981 tttctctttc tattattcca tggacttcat ttactgggtt taacttaaat atcaataata
     2041 atagtaatta ccttctaccc attattacag caggaaaatt cattaataaa ggtaattcaa
     2101 tatatttacc gctatcttta caggtacatc attctgtttg tgatggttat catgcaggat
     2161 tgtttatgaa ctctattcag gaattgtcag ataggcctaa tgactggctt ttataatatg
     2221 agataatgcc gactgtactt tttacagtcg gttttctaat gtcactaacc tgccccgtta
     2281 gttgaagaag gtttttatat tacagctcca gatcctctac gccggacgca tcgtggccgg
     2341 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
     2401 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
     2461 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
     2521 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
     2581 agagcgtcga catggatgag cgatgatgat atccgtttag gctgggcggt gatagcttct
     2641 cgttcaggca gtacgcctct tttcttttcc agacctgagg gaggcggaaa tggtgtgagg
     2701 ttccccagat ctgggggaaa agccaaatag gcgatcgcgg gagtgcttta tttgaagatc
     2761 aggctatcac tgcggtcaat agatttcaca atgtgatggc tggacagcct gaggaactct
     2821 cgaacccgaa tggaaacaac cagatattta tgaatcagcg cggctcacat ggcgttgtgc
     2881 tggcaaatgc aggttcatcc tctgtctcta tcaatacggc aacaaaattg cctgatggca
     2941 ggtatgacaa taaagctgga gcgggttcat ttcaagtgaa cgatggtaaa ctgacaggca
     3001 cgatcaatgc caggtctgta gctgtgcttt atcctgatga tattgcaaaa gcgcctcatg
     3061 ttttccttga gaattacaaa acaggtgtaa cacattcttt caatgatcaa ctgacgatta
     3121 ccttgcgtgc agatgcgaat acaacaaaag ccgtttatca aatcaataat ggaccagacg
     3181 acaggcgttt aaggatggag atcaattcac aatcggaaaa ggagatccaa tttggcaaaa
     3241 catacaccat catgttaaaa ggaacgaaca gtgatggtgt aacgaggacc gagaaataca
     3301 gttttgttaa aagagatcca gcgtcggcca aaaccatcgg ctatcaaaat ccgaatcatt
     3361 ggagccaggt aaatgcttat atctataaac atgatgggag ccgagtaatt gaattgaccg
     3421 gatcttggcc tggaaaacca atgactaaaa atgcagacgg aatttacacg ctgacgctgc
     3481 ctgcggacac ggatacaacc aacgcaaaag tgatttttaa taatggcagc gcccaagtgc
     3541 ccggtcagaa tcagcctggc tttgattacg tgctaaatgg tttatataat gactcgggct
     3601 taagcggttc tcttccccat tgagggcaag gctagacggg acttaccgaa agaaaccatc
     3661 aatgatggtt tcttttttgt tcataaatca gacaaaactt ttctcttgca aaagtttgtg
     3721 aagtgttgca caatataaat gtgaaatact tcacaaacaa aaagacatca aagagaaaca
     3781 taccctgcaa ggatgctgat attgtctgca tttgcgccgg agcaaaccaa aaacctggtg
     3841 agacacgcct tgaatttgac ggcattttct tagtcgcgac gcgaggctgg atggccttcc
     3901 ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt
     3961 ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca
     4021 gcctaacttc gatcactgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca
     4081 catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt
     4141 tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc
     4201 taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc
     4261 gcaaaccaac ccttggcaga acatatccat cgcgtccgcc atctccagca gccgcacgcg
     4321 gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt
     4381 gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat caccgatacg
     4441 cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat
     4501 ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat
     4561 tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtggaacac ctacatctgt
     4621 attaacgaag cgctggcatt gaccctgagt gatttttctc tggtcccgcc gcatccatac
     4681 cgccagttgt ttaccctcac aacgttccag taaccgggca tgttcatcat cagtaacccg
     4741 tatcgtgagc atcctctctc gtttcatcgg tatcattacc cccatgaaca gaaatccccc
     4801 ttacacggag gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg cccgctttat
     4861 cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg atgaacaggc
     4921 agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct gcctcgcgcg
     4981 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     5041 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     5101 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
     5161 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     5221 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     5281 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     5341 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     5401 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     5461 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     5521 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     5581 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     5641 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     5701 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     5761 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     5821 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     5881 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     5941 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     6001 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     6061 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     6121 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     6181 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     6241 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     6301 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     6361 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     6421 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     6481 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     6541 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     6601 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     6661 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     6721 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     6781 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     6841 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     6901 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     6961 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     7021 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     7081 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     7141 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     7201 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
     7261 gaatt
//
