LOCUS       40924_10471        5291 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCFB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Felts K, Vaillancourt P, Grafsky AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2000) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 5291)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5291)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (05-OCT-2000) Technical Services, Stratagene, 11011 N. Torrey Pines 
            Rd., La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        268..571
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        572..773
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    791
                     /label=transcription initiation start site
                     /note="transcription initiation start site"
     regulatory      993..997
                     /label=SD
                     /note="SD"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    998..1355
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1420..1836
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     misc_feature    1846..2219
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     misc_feature    2232..2254
                     /label=multiple cloning site
                     /note="multiple cloning site"
     LTR             2338..2931
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     rep_origin      3044
                     /label=ColE1 replication origin
                     /note="ColE1 replication origin"
     misc_feature    3142..3282
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3467..4055)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4229..5086)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5087..5191)
                     /label=AmpR promoter
ORIGIN
        1 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc
       61 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta
      121 ccctattccc cacactcacc ggagccaaag ccgcgggaca tatacatgtg aaagacccca
      181 cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat
      241 aacgcatgcc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      301 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      361 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      421 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      481 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      541 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      601 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      661 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      721 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagatgcatc
      781 ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata
      841 aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg
      901 agtgattgac tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga
      961 cccctgccca gggaccaccg acccaccacc gggaggtaag ctggccagca acttatctgt
     1021 gtctgtccga ttgtctagtg tctatgactg attttatgcg cctgcgtcgg tactagttag
     1081 ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttcgga acacccggcc
     1141 gcaaccctgg gagacgtccc agggacttcg ggggccgttt ttgtggcccg acctgagtcc
     1201 aaaaatcccg atcgttttgg actctttggt gcacccccct tagaggaggg atatgtggtt
     1261 ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt
     1321 tgggaccgaa gccgcgccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
     1381 ctgactgtgt ttctgtattt gtctgaaaat atgggcccgg gccagactgt taccactccc
     1441 ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta
     1501 gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc
     1561 ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc
     1621 ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc
     1681 ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct
     1741 cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc
     1801 tccctttatc cagccctcac tccttctcta ggcgccccca tatggccata tgagatctta
     1861 tatggggcac ccccgcccct tgtaaacttc cctgaccctg acatgacaag agttactaac
     1921 agcccctctc tccaagctca cttacaggct ctctacttag tccagcacga agtctggaga
     1981 cctctggcgg cacgtaccaa gaacaactgg accgaccggt ggtacctcac ccttaccgag
     2041 tcggcgacac agtgtgggtc cgccgacacc agactaagaa cctagaacct cgctggaaag
     2101 gaccttacac agtcctgctg accaccccca ccgccctcaa agtagacggc atcgcagctt
     2161 ggatacacgc cgcccacgtg aaggctgccg accccggggg tggaccatcc tctagactgc
     2221 cggatcgaat tgtcgacgaa ttcggatcct cgagcggccg cgatccgagt tcttctgagc
     2281 gggactctgg ggttcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat
     2341 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg
     2401 gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc
     2461 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
     2521 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
     2581 cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga
     2641 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga
     2701 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata
     2761 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt
     2821 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt
     2881 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat ttgggggctc
     2941 gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg
     3001 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     3061 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     3121 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     3181 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     3241 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     3301 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     3361 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     3421 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt cataggctcc
     3481 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     3541 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3601 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     3661 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     3721 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3781 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3841 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3901 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3961 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4021 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4081 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     4141 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     4201 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     4261 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     4321 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     4381 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     4441 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     4501 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac
     4561 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     4621 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     4681 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     4741 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4801 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
     4861 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4921 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4981 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     5041 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     5101 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     5161 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     5221 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     5281 ttcgtcttca a
//
