LOCUS       40924_10456        8356 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Chloroplast expression vector pCEV3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8356)
  AUTHORS   Chen P-Y., To K-Y.
  TITLE     Construction of chloroplast expression vectors for higher plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8356)
  AUTHORS   Chen P-Y., To K-Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2017) Agricultural Biotechnology Research Center, 
            Academia Sinica, No. 128, Academia Road, Section 2, Nankang, Taipei 
            11529, Taiwan
REFERENCE   3  (bases 1 to 8356)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8356)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-APR-2017) Agricultural Biotechnology Research Center, Academia 
            Sinica, No. 128, Academia Road, Section 2, Nankang, Taipei 11529, 
            Taiwan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(26..42)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(50..66)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(74..104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(119..140)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(428..1016)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1190..2047)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2048..2152)
                     /label=AmpR promoter
     primer_bind     2626..2642
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2655..4648
                     /note="tomato left targeting region; contains 16S rRNA and
                     trnI"
     rep_origin      4072..4153
                     /note="chloroplast replication origin; oriA"
     regulatory      4655..4902
                     /note="atpI promoter; PatpI"
                     /regulatory_class="promoter"
     misc_feature    4903..4918
                     /label=cloning site
                     /note="cloning site"
     regulatory      4919..5087
                     /note="psbA terminator; TpsbA"
                     /regulatory_class="terminator"
     regulatory      5094..5312
                     /note="rrn promoter; Prrn"
                     /regulatory_class="promoter"
     CDS             5319..6110
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      6120..6341
                     /note="psbA terminator; TpsbA"
                     /regulatory_class="terminator"
     misc_feature    6348..8351
                     /note="tomato right targeting region; contains trnA and 23S
                     rRNA"
ORIGIN
        1 ggcatgcaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
       61 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
      121 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
      181 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
      241 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      301 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      361 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      421 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      481 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      541 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      601 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      661 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      721 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      781 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
      841 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
      901 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
      961 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1021 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1081 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     1141 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     1201 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     1261 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     1321 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     1381 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     1441 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     1501 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     1561 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     1621 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     1681 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     1741 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     1801 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     1861 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     1921 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     1981 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     2041 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     2101 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     2161 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     2221 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     2281 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     2341 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     2401 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     2461 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
     2521 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     2581 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     2641 gtgaattcga gctctaccaa gctggagtac ggtaggggca gagggaattt ccggtggagc
     2701 ggtgaaatgc gtagagatcg gaaagaacac caacggcgaa agcactctgc tgggccgaca
     2761 ctgacactga gagacgaaag ctaggggagc gaatgggatt agatacccca gtagtcctag
     2821 ccgtaaacga tggatactag gcgctgtgcg tatcgacccg tgcagtgctg tagctaacgc
     2881 gttaagtatc ccgcctgggg agtacgttcg caagaatgaa actcaaagga attgacgggg
     2941 gcccgcacaa gcggtggagc atgtggttta attcgatgca aagcgaagaa ccttaccagg
     3001 gcttgacatg ccgcgaatcc tcttgaaaga gaggggtgcc ttcgggaacg cggacacagg
     3061 tggtgcatgg ctgtcgtcag ctcgtgccgt aaggtgttgg gttaagtccc gcaacgagcg
     3121 caaccctcgt gtttagttgc catcgttgag tttggaaccc tgaacagact gccggtgata
     3181 agccggagga aggtgaggat gacgtcaagt catcatgccc cttatgccct gggcgacaca
     3241 cgtgctacaa tggccgggac aaagggtcgc gatcccgcga gggtgagcta accccaaaaa
     3301 cccgtcctca gttcggattg caggctgcaa ctcgcctgca tgaagccgga atcgctagta
     3361 atcgccggtc agccatacgg cggtgaattc gttcccgggc cttgtacaca ccgcccgtca
     3421 cactatggga gctggccatg cccgaagtcg ttaccttaac cgcaaggagg gggatgccga
     3481 aggcagggct agtgactgga gtgaagtcgt aacaaggtag ccgtactgga aggtgcggct
     3541 ggatcacctc cttttcaggg agagctaatg cttgttgggt attttggttt gacactgctt
     3601 cacaccccca aaaaaaagaa gggagctacg tctgagttaa acttggagat ggaagtcttc
     3661 tttcctttct cgacggtgaa gtaagaccaa gctcatgagc ttattatcct aggtcggaac
     3721 aagttgatag gacccccttt tttacgtccc tatgttcccc ccgtgtggcg acatgggggc
     3781 gaaaaaagga aagagaggga tggggtttct ctcgcttttg gcatagcggg cccccagtgg
     3841 gaggctcgca cgacgggcta ttagctcagt ggtagagcgc gcccctgata attgcgtcgt
     3901 tgtgcctggg ctgtgagggc tctcagccac atggatagtt caatgtgctc atcggcgcct
     3961 gaccctgaga tgtggatcat ccaaggcaca ttagcatggc gtactcctcc tgttcgaacc
     4021 ggggtttgaa accaaactcc tcctcaggag gatagatggg gcgattcggg tgagatccaa
     4081 tgtagatcca actttcgatt cactcgtggg atccgggcgg tccggggggg accaccacgg
     4141 ctcctctctt ctcgagaatc catacatccc ttatcagtgt atggacagct atctctcgag
     4201 cacaggttta ggttcggcct caatgggaaa ataaaatgga gcacctaaca acgcatcttc
     4261 acagaccaag aactacgaga tcaccccttt cattctgggg tgacggaggg atcgtaccat
     4321 tcgagccgtt tttttcttga ctcgaaatcg aaatgggagc aggtttgaaa aaggatctta
     4381 gagtgtctag ggttgggcca ggagggtctc ttaacgcctt cttttttctt ctcatcggag
     4441 ttatttcaca aagacttgcc agggtaagga agaagggggg aacaagcaca cttggagagc
     4501 gcagtacaac ggagagttgt atgctgcgtt cgggaaggat gaatcgctcc cgaaaaggaa
     4561 tctattgatt ctctcccaat tggttggacc gtaggtgcga tgatttactt cacgggcgag
     4621 gtctctggtt caagtccagg atggcccaga gctctctagc tatataagaa atccttgatt
     4681 aataataaca taataagata aataacttac ttcagaaatc ccttatggaa tcgcttacta
     4741 tttctgaatt tcaaaaaaga gataaaaata gctggggata ttatgtgatt tattagtatt
     4801 ctaaatctta gttggtattc aaaatatccg attcaagtag acaaagagat ggttgaatca
     4861 aaaaattttg tttaaagttc aattttttca gagggcaagg cagcggccgc ggcgcgcctc
     4921 aaccgaaatt caattaagga aataaattaa ggaaatacaa aaaggggggt agtcatttgt
     4981 atataacttt gtatgacttt tctcttctat ttttttgtat ttcctccctt tccttttcta
     5041 tttgtatttt tttatcattg cttccattga attccgtgtt ctataacggt accatcagtt
     5101 cgagcctgat tatccctaag cccaatgtga gtttttctag ttggatttgc tcccccgccg
     5161 tcgttcaatg agaatggata agaggctcgt gggattgacg tgagggggca gggatggcta
     5221 tatttctggg agcgaactcc gggcgaatat gaagcgcatg gatacaagtt atgccttgga
     5281 atgaaagaca attccgaatc cgctttgtct acggatccat gattgaacaa gatggattgc
     5341 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     5401 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     5461 ttgtcaagac cgacctgtcc ggtgccctga atgaactcca agacgaggca gcgcggctat
     5521 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     5581 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     5641 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     5701 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     5761 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     5821 ccgaactgtt cgccaggctc aaggcgcgga tgcccgacgg cgaggatctc gtcgtgaccc
     5881 acggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     5941 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     6001 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     6061 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgatctagag
     6121 atcctggcct agtctatagg aggttttgaa aagaaaggag caataatcat tttcttgttc
     6181 tatcaagagg gtgctattgc tcctttcttt ttttcttttt atttatttac tagtatttta
     6241 cttacataga cttttttgtt tacattatag aaaaagaagg agaggttatt ttcttgcatt
     6301 tattcatgat tgagtattct attttgattt tgtatttgtt tctgcagctg cgccagggaa
     6361 aagaatagaa gaagcatctg actacttcat gcatgctcca cttggctcgg ggggatatag
     6421 ctcagttggt agagctccgc tcttgcaatt gggtcgttgc gattacgggt tggatgtcta
     6481 attgtccagg cggtaatgat agtatcttgt acctgaaccg gtggctcact ttttctaagt
     6541 aatggggaag aggaccgaaa cgtgccactg aaagactcta ctgagacaaa gatgggctgt
     6601 caagaacgta gaggaggtag gatgggcagt tggtcagatc tagtatggat cgtacatgga
     6661 cggtagttgg agtcggcggc tctcccaggg ttccctcatc tgagatctct ggggaagagg
     6721 atcaagttgg cccttgcgaa cagcttgatg cactatctcc cttcaaccct ttgagcgaaa
     6781 tgcggcaaaa gaaaaggaag gaaaatccat ggaccgaccc catcatctcc accccgtagg
     6841 aactacgaga tcaccccaag gacgccttcg gcatccaggg gtcacggacc gaccatagaa
     6901 ccctgttcaa taagtggaac gcattagctg tccgctctca ggttgggcag tcagggtcgg
     6961 agaagggcaa tgactcattc ttaaaaccag cgttcttaag accaaagagt cgggcggaag
     7021 ggggggaaag ccctccgttc ctggttctcc tgtagttgga tcctccggaa ccacaagaat
     7081 ccttagttag aatgggattc caactcagca ccttttgagt gagattttga gaagagttgc
     7141 tctttggaga gcacagtacg atgaaagttg taagctgtgt tcggggggga gttattgtct
     7201 atcgttggcc tctatggtag aatcagtcgg gggacctgag aggcggtggt ttaccctgcg
     7261 gcggatgtca gcggttcgag tccgcttatc tccaactcgt gaacttagcc gatacaaagc
     7321 tttatgatag cacccaattt ttccgattcg gcggttcgat ctatgattta tcattcatgg
     7381 acgttgataa gatccatcca tttagcagca ccttaggatg gcatagcctt aaaagtgaag
     7441 ggcgaggttc aaacgaggaa aggcttacgg tggataccta ggcacccaga gacgaggaag
     7501 ggcgtagtaa tcgacgaaat gcttcgggga gttgaaaata agcatagatc cggagattcc
     7561 cgaatagggc aacctttcga actgctgctg aatccatggg caggcaagag acaacctggc
     7621 gaactgaaac atcttagtag ccagaggaaa agaaagcaaa agcgattccc gtagtagcgg
     7681 cgagcgaaat gggagcagcc taaaccgtga aaacggggtt gtgggagagc aatacaagcg
     7741 tcgtgctgct aggcgaagca gcctgaatgc tgcaccctag atggcgaaag tccagtagcc
     7801 gaaagcatca ctagcttacg ctctgacccg agtagcatgg ggcacgtgga atcccgtgtg
     7861 aatcagcaag gaccaccttg caaggctaaa tactcctggg tgaccgatag cgaagtagta
     7921 ccgtgaggga agggtgaaaa gaacccccat cggggagtga aatagaacat gaaaccgtaa
     7981 gctcccaagc agtgggagga gccagggctc tgaccgcgtg cctgttgaag aatgagccgg
     8041 cgactcatag gcagtggctt ggttaaggga acccaccgga gccgtagcga aagcgagtct
     8101 tcatagggca attgtcactg cttatggacc cgaacctggg tgatctatcc atgaccagga
     8161 tgaagcttgg gtgaaactaa gtggaggtcc gaaccgactg atgttgaaga atcagcggat
     8221 gagttgtggt taggggtgaa atgccactcg aacccagagc tagctggttc tccccgaaat
     8281 gcgttgaggc gcagcagttg actggacatc taggggtaaa gcactgtttc ggtgcgggcc
     8341 gcgagagcgg tctgca
//
