LOCUS       40924_10451        8353 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Chloroplast expression vector pCEV2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Chen P-Y., To K-Y.
  TITLE     Construction of chloroplast expression vectors for higher plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8353)
  AUTHORS   Chen P-Y., To K-Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2017) Agricultural Biotechnology Research Center, 
            Academia Sinica, No. 128, Academia Road, Section 2, Nankang, Taipei 
            11529, Taiwan
REFERENCE   3  (bases 1 to 8353)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8353)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-APR-2017) Agricultural Biotechnology Research Center, Academia 
            Sinica, No. 128, Academia Road, Section 2, Nankang, Taipei 11529, 
            Taiwan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(26..42)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(50..66)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(74..104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(119..140)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(428..1016)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1190..2047)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2048..2152)
                     /label=AmpR promoter
     primer_bind     2626..2642
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2655..4648
                     /note="tomato left targeting region; contains 16S rRNA and
                     trnI"
     rep_origin      4072..4153
                     /note="chloroplast replication origin; oriA"
     regulatory      4655..4902
                     /note="atpI promoter; PatpI"
                     /regulatory_class="promoter"
     misc_feature    4903..4918
                     /label=cloning site
                     /note="cloning site"
     regulatory      4919..5087
                     /note="rps16 terminator; Trps16"
                     /regulatory_class="terminator"
     regulatory      5094..5312
                     /note="rrn promoter; Prrn"
                     /regulatory_class="promoter"
     CDS             5319..6107
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     regulatory      6117..6338
                     /note="psbA terminator; TpsbA"
                     /regulatory_class="terminator"
     misc_feature    6345..8348
                     /note="tomato right targeting region; contains trnA and 23S
                     rRNA"
ORIGIN
        1 ggcatgcaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
       61 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
      121 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
      181 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
      241 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      301 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      361 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      421 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      481 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      541 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      601 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      661 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      721 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      781 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
      841 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
      901 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
      961 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1021 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1081 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     1141 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     1201 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     1261 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     1321 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     1381 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     1441 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     1501 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     1561 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     1621 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     1681 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     1741 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     1801 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     1861 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     1921 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     1981 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     2041 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     2101 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     2161 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     2221 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     2281 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     2341 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     2401 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     2461 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
     2521 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     2581 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     2641 gtgaattcga gctctaccaa gctggagtac ggtaggggca gagggaattt ccggtggagc
     2701 ggtgaaatgc gtagagatcg gaaagaacac caacggcgaa agcactctgc tgggccgaca
     2761 ctgacactga gagacgaaag ctaggggagc gaatgggatt agatacccca gtagtcctag
     2821 ccgtaaacga tggatactag gcgctgtgcg tatcgacccg tgcagtgctg tagctaacgc
     2881 gttaagtatc ccgcctgggg agtacgttcg caagaatgaa actcaaagga attgacgggg
     2941 gcccgcacaa gcggtggagc atgtggttta attcgatgca aagcgaagaa ccttaccagg
     3001 gcttgacatg ccgcgaatcc tcttgaaaga gaggggtgcc ttcgggaacg cggacacagg
     3061 tggtgcatgg ctgtcgtcag ctcgtgccgt aaggtgttgg gttaagtccc gcaacgagcg
     3121 caaccctcgt gtttagttgc catcgttgag tttggaaccc tgaacagact gccggtgata
     3181 agccggagga aggtgaggat gacgtcaagt catcatgccc cttatgccct gggcgacaca
     3241 cgtgctacaa tggccgggac aaagggtcgc gatcccgcga gggtgagcta accccaaaaa
     3301 cccgtcctca gttcggattg caggctgcaa ctcgcctgca tgaagccgga atcgctagta
     3361 atcgccggtc agccatacgg cggtgaattc gttcccgggc cttgtacaca ccgcccgtca
     3421 cactatggga gctggccatg cccgaagtcg ttaccttaac cgcaaggagg gggatgccga
     3481 aggcagggct agtgactgga gtgaagtcgt aacaaggtag ccgtactgga aggtgcggct
     3541 ggatcacctc cttttcaggg agagctaatg cttgttgggt attttggttt gacactgctt
     3601 cacaccccca aaaaaaagaa gggagctacg tctgagttaa acttggagat ggaagtcttc
     3661 tttcctttct cgacggtgaa gtaagaccaa gctcatgagc ttattatcct aggtcggaac
     3721 aagttgatag gacccccttt tttacgtccc tatgttcccc ccgtgtggcg acatgggggc
     3781 gaaaaaagga aagagaggga tggggtttct ctcgcttttg gcatagcggg cccccagtgg
     3841 gaggctcgca cgacgggcta ttagctcagt ggtagagcgc gcccctgata attgcgtcgt
     3901 tgtgcctggg ctgtgagggc tctcagccac atggatagtt caatgtgctc atcggcgcct
     3961 gaccctgaga tgtggatcat ccaaggcaca ttagcatggc gtactcctcc tgttcgaacc
     4021 ggggtttgaa accaaactcc tcctcaggag gatagatggg gcgattcggg tgagatccaa
     4081 tgtagatcca actttcgatt cactcgtggg atccgggcgg tccggggggg accaccacgg
     4141 ctcctctctt ctcgagaatc catacatccc ttatcagtgt atggacagct atctctcgag
     4201 cacaggttta ggttcggcct caatgggaaa ataaaatgga gcacctaaca acgcatcttc
     4261 acagaccaag aactacgaga tcaccccttt cattctgggg tgacggaggg atcgtaccat
     4321 tcgagccgtt tttttcttga ctcgaaatcg aaatgggagc aggtttgaaa aaggatctta
     4381 gagtgtctag ggttgggcca ggagggtctc ttaacgcctt cttttttctt ctcatcggag
     4441 ttatttcaca aagacttgcc agggtaagga agaagggggg aacaagcaca cttggagagc
     4501 gcagtacaac ggagagttgt atgctgcgtt cgggaaggat gaatcgctcc cgaaaaggaa
     4561 tctattgatt ctctcccaat tggttggacc gtaggtgcga tgatttactt cacgggcgag
     4621 gtctctggtt caagtccagg atggcccaga gctctctagc tatataagaa atccttgatt
     4681 aataataaca taataagata aataacttac ttcagaaatc ccttatggaa tcgcttacta
     4741 tttctgaatt tcaaaaaaga gataaaaata gctggggata ttatgtgatt tattagtatt
     4801 ctaaatctta gttggtattc aaaatatccg attcaagtag acaaagagat ggttgaatca
     4861 aaaaattttg tttaaagttc aattttttca gagggcaagg cagcggccgc ggcgcgcctc
     4921 aaccgaaatt caattaagga aataaattaa ggaaatacaa aaaggggggt agtcatttgt
     4981 atataacttt gtatgacttt tctcttctat ttttttgtat ttcctccctt tccttttcta
     5041 tttgtatttt tttatcattg cttccattga attccgtgtt ctataacggt accatcagtt
     5101 cgagcctgat tatccctaag cccaatgtga gtttttctag ttggatttgc tcccccgccg
     5161 tcgttcaatg agaatggata agaggctcgt gggattgacg tgagggggca gggatggcta
     5221 tatttctggg agcgaactcc gggcgaatat gaagcgcatg gatacaagtt atgccttgga
     5281 atgaaagaca attccgaatc cgctttgtct acggatccat gagggaagcg gtgatcgccg
     5341 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     5401 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
     5461 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     5521 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     5581 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     5641 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     5701 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     5761 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     5821 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     5881 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     5941 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     6001 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     6061 aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tctagagatc
     6121 ctggcctagt ctataggagg ttttgaaaag aaaggagcaa taatcatttt cttgttctat
     6181 caagagggtg ctattgctcc tttctttttt tctttttatt tatttactag tattttactt
     6241 acatagactt ttttgtttac attatagaaa aagaaggaga ggttattttc ttgcatttat
     6301 tcatgattga gtattctatt ttgattttgt atttgtttct gcagctgcgc cagggaaaag
     6361 aatagaagaa gcatctgact acttcatgca tgctccactt ggctcggggg gatatagctc
     6421 agttggtaga gctccgctct tgcaattggg tcgttgcgat tacgggttgg atgtctaatt
     6481 gtccaggcgg taatgatagt atcttgtacc tgaaccggtg gctcactttt tctaagtaat
     6541 ggggaagagg accgaaacgt gccactgaaa gactctactg agacaaagat gggctgtcaa
     6601 gaacgtagag gaggtaggat gggcagttgg tcagatctag tatggatcgt acatggacgg
     6661 tagttggagt cggcggctct cccagggttc cctcatctga gatctctggg gaagaggatc
     6721 aagttggccc ttgcgaacag cttgatgcac tatctccctt caaccctttg agcgaaatgc
     6781 ggcaaaagaa aaggaaggaa aatccatgga ccgaccccat catctccacc ccgtaggaac
     6841 tacgagatca ccccaaggac gccttcggca tccaggggtc acggaccgac catagaaccc
     6901 tgttcaataa gtggaacgca ttagctgtcc gctctcaggt tgggcagtca gggtcggaga
     6961 agggcaatga ctcattctta aaaccagcgt tcttaagacc aaagagtcgg gcggaagggg
     7021 gggaaagccc tccgttcctg gttctcctgt agttggatcc tccggaacca caagaatcct
     7081 tagttagaat gggattccaa ctcagcacct tttgagtgag attttgagaa gagttgctct
     7141 ttggagagca cagtacgatg aaagttgtaa gctgtgttcg ggggggagtt attgtctatc
     7201 gttggcctct atggtagaat cagtcggggg acctgagagg cggtggttta ccctgcggcg
     7261 gatgtcagcg gttcgagtcc gcttatctcc aactcgtgaa cttagccgat acaaagcttt
     7321 atgatagcac ccaatttttc cgattcggcg gttcgatcta tgatttatca ttcatggacg
     7381 ttgataagat ccatccattt agcagcacct taggatggca tagccttaaa agtgaagggc
     7441 gaggttcaaa cgaggaaagg cttacggtgg atacctaggc acccagagac gaggaagggc
     7501 gtagtaatcg acgaaatgct tcggggagtt gaaaataagc atagatccgg agattcccga
     7561 atagggcaac ctttcgaact gctgctgaat ccatgggcag gcaagagaca acctggcgaa
     7621 ctgaaacatc ttagtagcca gaggaaaaga aagcaaaagc gattcccgta gtagcggcga
     7681 gcgaaatggg agcagcctaa accgtgaaaa cggggttgtg ggagagcaat acaagcgtcg
     7741 tgctgctagg cgaagcagcc tgaatgctgc accctagatg gcgaaagtcc agtagccgaa
     7801 agcatcacta gcttacgctc tgacccgagt agcatggggc acgtggaatc ccgtgtgaat
     7861 cagcaaggac caccttgcaa ggctaaatac tcctgggtga ccgatagcga agtagtaccg
     7921 tgagggaagg gtgaaaagaa cccccatcgg ggagtgaaat agaacatgaa accgtaagct
     7981 cccaagcagt gggaggagcc agggctctga ccgcgtgcct gttgaagaat gagccggcga
     8041 ctcataggca gtggcttggt taagggaacc caccggagcc gtagcgaaag cgagtcttca
     8101 tagggcaatt gtcactgctt atggacccga acctgggtga tctatccatg accaggatga
     8161 agcttgggtg aaactaagtg gaggtccgaa ccgactgatg ttgaagaatc agcggatgag
     8221 ttgtggttag gggtgaaatg ccactcgaac ccagagctag ctggttctcc ccgaaatgcg
     8281 ttgaggcgca gcagttgact ggacatctag gggtaaagca ctgtttcggt gcgggccgcg
     8341 agagcggtct gca
//
