LOCUS       40924_10446        8375 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Chloroplast expression vector pCEV1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8375)
  AUTHORS   Chen P-Y., To K-Y.
  TITLE     Construction of chloroplast expression vectors for higher plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8375)
  AUTHORS   Chen P-Y., To K-Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2017) Agricultural Biotechnology Research Center, 
            Academia Sinica, No. 128, Academia Road, Section 2, Nankang, Taipei 
            11529, Taiwan
REFERENCE   3  (bases 1 to 8375)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8375)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-APR-2017) Agricultural Biotechnology Research Center, Academia 
            Sinica, No. 128, Academia Road, Section 2, Nankang, Taipei 11529, 
            Taiwan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      2..249
                     /note="atpI promoter; PatpI"
                     /regulatory_class="promoter"
     misc_feature    250..265
                     /label=cloning site
                     /note="cloning site"
     regulatory      266..434
                     /note="rps16 terminator; Trps16"
                     /regulatory_class="terminator"
     regulatory      441..660
                     /note="rrn promoter; Prrn"
                     /regulatory_class="promoter"
     CDS             666..1454
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     regulatory      1464..1685
                     /note="psbA terminator; TpsbA"
                     /regulatory_class="terminator"
     misc_feature    1692..3707
                     /note="spinach right targeting region; contains trnA and
                     23S rRNA"
     primer_bind     complement(3738..3754)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3762..3778)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3786..3816)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3831..3852)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4140..4728)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4902..5759)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5760..5864)
                     /label=AmpR promoter
     primer_bind     6338..6354
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6367..8370
                     /note="spinach left target region; contains 16S rRNA and
                     trnI"
     rep_origin      7783..7864
                     /note="chloroplast replication origin; oriA"
ORIGIN
        1 ctctagctat ataagaaatc cttgattaat aataacataa taagataaat aacttacttc
       61 agaaatccct tatggaatcg cttactattt ctgaatttca aaaaagagat aaaaatagct
      121 ggggatatta tgtgatttat tagtattcta aatcttagtt ggtattcaaa atatccgatt
      181 caagtagaca aagagatggt tgaatcaaaa aattttgttt aaagttcaat tttttcagag
      241 ggcaaggcag cggccgcggc gcgcctcaac cgaaattcaa ttaaggaaat aaattaagga
      301 aatacaaaaa ggggggtagt catttgtata taactttgta tgacttttct cttctatttt
      361 tttgtatttc ctccctttcc ttttctattt gtattttttt atcattgctt ccattgaatt
      421 ccgtgttcta taacggtacc atcagttcga gcctgattat ccctaagccc aatgtgagtt
      481 tttctagttg gatttgctcc cccgccgtcg ttcaatgaga atggataaga ggctcgtggg
      541 attgacgtga gggggcaggg atggctatat ttctgggagc gaactccggg cgaatatgaa
      601 gcgcatggat acaagttatg ccttggaatg aaagacaatt ccgaatccgc tttgtctacg
      661 gatccatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
      721 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
      781 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
      841 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
      901 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
      961 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     1021 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     1081 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     1141 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     1201 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     1261 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     1321 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     1381 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     1441 aggtagtcgg caaataatct agagatcctg gcctagtcta taggaggttt tgaaaagaaa
     1501 ggagcaataa tcattttctt gttctatcaa gagggtgcta ttgctccttt ctttttttct
     1561 ttttatttat ttactagtat tttacttaca tagacttttt tgtttacatt atagaaaaag
     1621 aaggagaggt tattttcttg catttattca tgattgagta ttctattttg attttgtatt
     1681 tgtttctgca gctgcgccag ggaaaagaat aagaatcgaa gaagcgtctg actccttcat
     1741 gcatgctcca cttggctcgg ggggatatag ctcagttggt agagcgccgc tcttgcaatt
     1801 gggtcgttgc gattacgggt tggatgtcta attgtccagg cggtaatgat agtatcttgt
     1861 acctgaacca gtggctcact ttttctaagt aatggggaag aggaccaaaa catgccactg
     1921 aaagactcta ctgagacaaa acaaagatgg gctgtcaaga acgtagagga ggtaggatgg
     1981 gcagttggtc agatctagta tggatcgtac atggatagta gttggagtcg gcggctctcc
     2041 tggggttccc taatcttgga tcctggggaa gaggatcaag ttggcccttg cgaacaactt
     2101 gatgcactat ctcccttcaa ccctttgagc gaaatgcggc aaaaggaagg aaaatccatg
     2161 gaccgacccc atcgtctcca ccccgtagga actacgagat caccccaagg acgccttcgg
     2221 catccagggg tcacgggccg accatagaac cctgttcaat tcaataagtg gaacgcatta
     2281 gctgtccgct cccaggttgg gcagtaaggg tcggagaagg gcaatcactc attcttaaaa
     2341 ccagtattct taagaccaag gggtcgagcg gaaaaggggg gaaagctctc tgttcctggt
     2401 tctcctgtaa ctggatcctc cggaaccaca agaatcctta gttcgaattg gattccaact
     2461 tatcaccctt tttttgagat tttgagaaga gttgctcttt ggagagcaca gtacgatgaa
     2521 agttgtaagc tgtgttcggg ggggagttat tgtctatcgt tggcctctat ggtagaatca
     2581 tacagtcggg ggcctgagag acggtggttt accctgtggc ggatgtcagc ggttcgagtc
     2641 cgcttatctc caactcgtga acttagccag ttatatgata gcacccaatt tttccgattc
     2701 ggtcgttcga tctatgattt atcattcatg gacgttgata agatccttcc atttatattt
     2761 agcagcacct taggatggca taaccttaaa gttaagggcg aggttcaaac gaggaaaggc
     2821 ttacggtgga tacctaggca cccagagacg aggaagggcg tattaatcga cgaaatgctt
     2881 cggggagttg aaaataagca gagatccgga gattcccgaa taggtcaacc tttcgaactt
     2941 ctgctgaatc catgggcagg caagagacaa cctggcgaac tgaaacatct tagtagccag
     3001 aggaaaagaa agcaaaagcg attcccgtag tagcggcgag cgaaatggga gcagcctaaa
     3061 ccgtgaaaac ggggttgtgg gagagcaata caagcgtcgt gctgctaggc gaatcagtgg
     3121 agtgcggaac cctagatggt gaaagtccag tagccgaaag catcactagc ttatgctctg
     3181 acccgagtag catggggcac gtggaatccc gtgtgaatca gcaaggacca ccttgcaagg
     3241 ctaaatactc ctgggtgacc gatagcgaag tagtaccgtg agggaagggt gaaaagaacc
     3301 cccatcgggg agtgaaatag aacatgaaac cgtaagctct caagcagtgg gagggggacc
     3361 agaccctgac cgcgtgcctg ttgaagaatg agccggcgac tcataggcag tggcttggtt
     3421 aagggaaccc accggagccg tagcgaaagc gagtcttcat agggcaattg tcactgctta
     3481 tggacccgaa cctgggtgat ctatccatga ccaggatgaa gcttgggtga aactaagtgg
     3541 aggtccgaac cgactgatgt tgaagaatca gcggatgagt tgtggttagg ggtgaaatgc
     3601 cactcgaacc cagagctagc tggttctccc cgaaatgcgt tgaggcgcag cagttgactg
     3661 gacatctagg ggtaaagcac tgtttcggtg cgggccgcga gagcggtctg caggcatgca
     3721 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     3781 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     3841 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     3901 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     3961 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     4021 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     4081 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     4141 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     4201 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4261 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4321 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4381 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4441 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4501 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4561 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4621 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4681 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4741 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4801 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     4861 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     4921 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     4981 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     5041 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     5101 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     5161 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     5221 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     5281 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     5341 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     5401 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     5461 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     5521 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     5581 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     5641 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     5701 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     5761 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     5821 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     5881 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     5941 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     6001 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     6061 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     6121 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     6181 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     6241 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     6301 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattc
     6361 gagctctacc aagctggagt acggtagggg cagagggaat ttccggtgga gcggtgaaat
     6421 gcgtagagat cggaaagaac accaacggcg aaagcactct gctgggccga cactgacact
     6481 gagagacgaa agctagggga gcgaatggga ttagataccc cagtagtcct agccgtaaac
     6541 gatggatact aggcgctgtg cgtatcgacc cgtgcagtgt tgtagctaac gcgttaagta
     6601 tcccgcctgg ggagtacgtt cgcaagaatg aaactcaaag gaattgacgg gggcccgcac
     6661 aagcggtgga gcatgtggtt taattcgatg caaagcgaag aaccttacca gggcttgaca
     6721 tgccgcgaat cctcttgaaa gagaggggtg ccttcgggaa cgcggacaca ggtggtgcat
     6781 ggctgtcgtc agctcgtgcc gtaaggtgtt gggttaagtc ccgcaacgag cgcaaccctc
     6841 gtgtttagtt gccaacgttg agtttggaac cctgaacaga ctgccggtga taagccggag
     6901 gaaggtgagg atgacgtcaa gtcatcatgc cccttatgcc ctgggcgaca cacgtgctac
     6961 aatggccggg acaaagggtc gcgatcccgc gagggtgagc taaccccaaa aacccgtcct
     7021 cagttcggat tgcaggctgc aactcgcctg catgaagccg gaatcgctag taatcgccgg
     7081 tcagccatac ggcggtgaat tcgttcccgg gccttgtaca caccgcccgt cacactatgg
     7141 gagctggcca tgcccgaagt cgttacctta accgcaagga gggggatgcc gaaggcaggg
     7201 ctagtgactg gagtgaagtc gtaacaaggt agccgtactg gaaggtgcgg ctggatcacc
     7261 tccttttcag ggagagctaa tgcttgttgg gtattttggt ttgacactgc ttcacaccca
     7321 aaaagaggcg agcgacgtct gagttaaact taaacttaaa cttggagttg gaagtcttct
     7381 ttcgtttctc ggcggtgaag taagaccaag ctcatgggct tattatccta ggtcggaaca
     7441 ataggatccc cttttttgcc tccatgtcgc cacacagggg gacacgggga cgtaaaaagg
     7501 aaagagaggg atggggtttc cctcgctttt ggcatagtgg gcctcccgct gggggcccgc
     7561 acgacgggct attagctcag tggtagagcg cgcccctgat aattgcgtcg ttgtgcctgg
     7621 gctgtgaggg ctctcaacca catggatagt tcaatgtgcc catcagcgcc tgaccttgag
     7681 atgtggatca tccaaggcac attagcatgg cgtactcctc ctgttcgaac cggggtttga
     7741 aaccaaactt ctcctcagga ggatagatgg ggcgattcag gtgagatcca atgtagatcc
     7801 aactttcgat tcactcgtgg gatccgggcg gtccgggggg gaccactacg gctcctctct
     7861 tatcgagaat ccatacatcc cttatcagtg tatggacagc tatctctcga gcacaggttt
     7921 aggttcggcc tcaatggaat tggagcacct aacaacgcat cttcacagac caagaactac
     7981 gagatcaccc ctttcattca ttttggggtg acggaaggat cgtaccattc gagccttttt
     8041 tcatgctttc ccagctttct cttgactcga aatgggagca ggtttgaaaa aggatcttag
     8101 agtgtctagg gttgggccag gagggtctct taatgccttc ttttttcttc tcatcggagt
     8161 tatttcacaa agacttgcca tggtaatatg gaagaagggg ggaacaagca cacttggaga
     8221 gcgcagtaca acggagagtt gtatgctgcg ttcgggaagg atgaatcgct cccaaaaagg
     8281 aatctattga ttctctccaa attggttgga ccgtaggtgc gatgatttac ttcacgggcg
     8341 aggtctctgg ttcaagtcca ggatggccca ggtac
//
