LOCUS       40924_10396       10382 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCEP4FLAG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10382)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10382)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 10382)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10382)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             418..2340
                     /codon_start=1
                     /label=EBNA1
                     /note="Epstein-Barr nuclear antigen 1, also known as
                     EBNA-1"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     rep_origin      2649..4438
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     polyA_signal    5158..5279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5296..5319)
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     promoter        complement(5379..5582)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(5583..5962)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    complement(6615..6663)
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(6731..7753)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(7793..7938)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     rep_origin      complement(8555..9143)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9317..10174)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10175..10279)
                     /label=AmpR promoter
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctgatcctc acaggccgca cccagctttt
       61 cttccgttgc cccagtagca tctctgtctg gtgaccttga agaggaagag gaggggtccc
      121 gagaatcccc atccctaccg tccagcaaaa agggggacga ggaatttgag gcctggcttg
      181 aggctcagga cgcaaatctt gaggatgttc agcgggagtt ttccgggctg cgagtaattg
      241 gtgatgagga cgaggatggt tcggaggatg gggaattttc agacctggat ctgtctgaca
      301 gcgaccatga aggggatgag ggtggggggg ctgttggagg gggcaggagt ctgcactccc
      361 tgtattcact gagcgtcgtc taataaagat gtctattgat ctcttttagt gtgaatcatg
      421 tctgacgagg ggccaggtac aggacctgga aatggcctag gagagaaggg agacacatct
      481 ggaccagaag gctccggcgg cagtggacct caaagaagag ggggtgataa ccatggacga
      541 ggacggggaa gaggacgagg acgaggaggc ggaagaccag gagccccggg cggctcagga
      601 tcagggccaa gacatagaga tggtgtccgg agaccccaaa aacgtccaag ttgcattggc
      661 tgcaaaggga cccacggtgg aacaggagca ggagcaggag cgggaggggc aggagcagga
      721 ggggcaggag caggaggagg ggcaggagca ggaggagggg caggaggggc aggaggggca
      781 ggaggggcag gagcaggagg aggggcagga gcaggaggag gggcaggagg ggcaggaggg
      841 gcaggagcag gaggaggggc aggagcagga ggaggggcag gaggggcagg agcaggagga
      901 ggggcaggag gggcaggagg ggcaggagca ggaggagggg caggagcagg aggaggggca
      961 ggaggggcag gagcaggagg aggggcagga ggggcaggag gggcaggagc aggaggaggg
     1021 gcaggagcag gaggggcagg aggggcagga ggggcaggag caggaggggc aggagcagga
     1081 ggaggggcag gaggggcagg aggggcagga gcaggagggg caggagcagg aggggcagga
     1141 gcaggagggg caggagcagg aggggcagga ggggcaggag caggaggggc aggaggggca
     1201 ggagcaggag gggcaggagg ggcaggagca ggaggagggg caggaggggc aggagcagga
     1261 ggaggggcag gaggggcagg agcaggaggg gcaggagggg caggagcagg aggggcagga
     1321 ggggcaggag caggaggggc aggaggggca ggagcaggag gaggggcagg agcaggaggg
     1381 gcaggagcag gaggtggagg ccggggtcga ggaggcagtg gaggccgggg tcgaggaggt
     1441 agtggaggcc ggggtcgagg aggtagtgga ggccgccggg gtagaggacg tgaaagagcc
     1501 agggggggaa gtcgtgaaag agccaggggg agaggtcgtg gacgtggaga aaagaggccc
     1561 aggagtccca gtagtcagtc atcatcatcc gggtctccac cgcgcaggcc ccctccaggt
     1621 agaaggccat ttttccaccc tgtaggggaa gccgattatt ttgaatacca ccaagaaggt
     1681 ggcccagatg gtgagcctga cgtgcccccg ggagcgatag agcagggccc cgcagatgac
     1741 ccaggagaag gcccaagcac tggaccccgg ggtcagggtg atggaggcag gcgcaaaaaa
     1801 ggagggtggt ttggaaagca tcgtggtcaa ggaggttcca acccgaaatt tgagaacatt
     1861 gcagaaggtt taagagctct cctggctagg agtcacgtag aaaggactac cgacgaagga
     1921 acttgggtcg ccggtgtgtt cgtatatgga ggtagtaaga cctcccttta caacctaagg
     1981 cgaggaactg cccttgctat tccacaatgt cgtcttacac cattgagtcg tctccccttt
     2041 ggaatggccc ctggacccgg cccacaacct ggcccgctaa gggagtccat tgtctgttat
     2101 ttcatggtct ttttacaaac tcatatattt gctgaggttt tgaaggatgc gattaaggac
     2161 cttgttatga caaagcccgc tcctacctgc aatatcaggg tgactgtgtg cagctttgac
     2221 gatggagtag atttgcctcc ctggtttcca cctatggtgg aaggggctgc cgcggagggt
     2281 gatgacggag atgacggaga tgaaggaggt gatggagatg agggtgagga agggcaggag
     2341 tgatgtaact tgttaggaga cgccctcaat cgtattaaaa gccgtgtatt cccccgcact
     2401 aaagaataaa tccccagtag acatcatgcg tgctgttggt gtatttctgg ccatctgtct
     2461 tgtcaccatt ttcgtcctcc caacatgggg caattgggca tacccatgtt gtcacgtcac
     2521 tcagctccgc gctcaacacc ttctcgcgtt ggaaaacatt agcgacattt acctggtgag
     2581 caatcagaca tgcgacggct ttagcctggc ctccttaaat tcacctaaga atgggagcaa
     2641 ccagcatgca ggaaaaggac aagcagcgaa aattcacgcc cccttgggag gtggcggcat
     2701 atgcaaagga tagcactccc actctactac tgggtatcat atgctgactg tatatgcatg
     2761 aggatagcat atgctacccg gatacagatt aggatagcat atactaccca gatatagatt
     2821 aggatagcat atgctaccca gatatagatt aggatagcct atgctaccca gatataaatt
     2881 aggatagcat atactaccca gatatagatt aggatagcat atgctaccca gatatagatt
     2941 aggatagcct atgctaccca gatatagatt aggatagcat atgctaccca gatatagatt
     3001 aggatagcat atgctatcca gatatttggg tagtatatgc tacccagata taaattagga
     3061 tagcatatac taccctaatc tctattagga tagcatatgc tacccggata cagattagga
     3121 tagcatatac tacccagata tagattagga tagcatatgc tacccagata tagattagga
     3181 tagcctatgc tacccagata taaattagga tagcatatac tacccagata tagattagga
     3241 tagcatatgc tacccagata tagattagga tagcctatgc tacccagata tagattagga
     3301 tagcatatgc tatccagata tttgggtagt atatgctacc catggcaaca ttagcccacc
     3361 gtgctctcag cgacctcgtg aatatgagga ccaacaaccc tgtgcttggc gctcaggcgc
     3421 aagtgtgtgt aatttgtcct ccagatcgca gcaatcgcgc ccctatcttg gcccgcccac
     3481 ctacttatgc aggtattccc cggggtgcca ttagtggttt tgtgggcaag tggtttgacc
     3541 gcagtggtta gcggggttac aatcagccaa gttattacac ccttatttta cagtccaaaa
     3601 ccgcagggcg gcgtgtgggg gctgacgcgt gcccccactc cacaatttca aaaaaaagag
     3661 tggccacttg tctttgttta tgggccccat tggcgtggag ccccgtttaa ttttcggggg
     3721 tgttagagac aaccagtgga gtccgctgct gtcggcgtcc actctctttc cccttgttac
     3781 aaatagagtg taacaacatg gttcacctgt cttggtccct gcctgggaca catcttaata
     3841 accccagtat catattgcac taggattatg tgttgcccat agccataaat tcgtgtgaga
     3901 tggacatcca gtctttacgg cttgtcccca ccccatggat ttctattgtt aaagatattc
     3961 agaatgtttc attcctacac tagtatttat tgcccaaggg gtttgtgagg gttatattgg
     4021 tgtcatagca caatgccacc actgaacccc ccgtccaaat tttattctgg gggcgtcacc
     4081 tgaaaccttg ttttcgagca cctcacatac accttactgt tcacaactca gcagttattc
     4141 tattagctaa acgaaggaga atgaagaagc aggcgaagat tcaggagagt tcactgcccg
     4201 ctccttgatc ttcagccact gcccttgtga ctaaaatggt tcactaccct cgtggaatcc
     4261 tgaccccatg taaataaaac cgtgacagct catggggtgg gagatatcgc tgttccttag
     4321 gaccctttta ctaaccctaa ttcgatagca tatgcttccc gttgggtaac atatgctatt
     4381 gaattagggt tagtctggat agtatatact actacccggg aagcatatgc tacccgttta
     4441 gggttaacaa gggggcctta taaacactat tgctaatgcc ctcttgaggg tccgcttatc
     4501 ggtagctaca caggcccctc tgattgacgt tggtgtagcc tcccgtagtc ttcctgggcc
     4561 cctgggaggt acatgtcccc cagcattggt gtaagagctt cagccaagag ttacacataa
     4621 aggcaatgtt gtgttgcagt ccacagactg caaagtctgc tccaggatga aagccactca
     4681 gtgttggcaa atgtgcacat ccatttataa ggatgtcaac tacagtcaga gaaccccttt
     4741 gtgtttggtc cccccccgtg tcacatgtgg aacagggccc agttggcaag ttgtaccaac
     4801 caactgaagg gattacatgc actgccccgc gaagaagggg cagagatgcc gtagtcaggt
     4861 ttagttcgtc cggcggcggg gctctagagt cgaccggtca tggctgcgcc ccgacacccg
     4921 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     4981 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     5041 gcgaggcagc cggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     5101 aaacctcccc acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     5161 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     5221 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     5281 catgtctggg atcctcttgt catcgtcgtc cttgtagtcc atggtagctt gctagcagct
     5341 ggtacccagc ttctagagat ctgacggttc actaaacgag ctctgcttat atagacctcc
     5401 caccgtacac gcctaccgcc catttgcgtc aacggggcgg ggttattacg acattttgga
     5461 aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt
     5521 ggaaatcccc gtgagtcaaa ccgctatcca cgcccattgg tgtactgcca aaaccgcatc
     5581 accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccgtaag
     5641 gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc
     5701 ggacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc
     5761 acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt
     5821 gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat
     5881 gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga ttactattaa
     5941 taactagtca ataatcaatg tcaacatggc ggtcatattg gacatgagcc aatataaatg
     6001 tacatattat gatatagata caacgtatgc aatggccaat agccaatatt gatttatgct
     6061 atataaccaa tgactaatat ggctaattgc caatattgat tcaatgtata gatcttccat
     6121 acctaccagt tctgcgcctg cagcaatgca acaacgttgc ccggatctgc gatgataagc
     6181 tgtcaaacat gagaattggt cgactagctt ggcacgccag aaatccgcgc ggtggttttt
     6241 gggggtcggg ggtgtttggc agccacagac gcccggtgtt cgtgtcgcgc cagtacatgc
     6301 ggtccatgcc caggccatcc aaaaaccatg ggtctgtctg ctcagtccag tcgtggacca
     6361 gaccccacgc aacgcccaaa ataataaccc ccacgaacca taaaccattc cccatggggg
     6421 accccgtccc taacccacgg ggccagtggc tatggcaggg cctgccgccc cgacgttggc
     6481 tgcgagccct gggccttcac ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg
     6541 ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg
     6601 aaccccgcgt ttatgaacaa acgacccaac acccgtgcgt tttattctgt ctttttattg
     6661 ccgtcatagc gcgggttcct tccggtattg tctccttccg tgtttcagtt agcctccccc
     6721 atctccccta ttcctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga
     6781 gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc
     6841 cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat
     6901 catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat
     6961 acgcccggag ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct
     7021 gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg
     7081 aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca
     7141 ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg
     7201 cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca
     7261 cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag
     7321 tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg
     7381 ccgcagcgat cgcatccatg gcctccgcga ccggctgcag aacagcgggc agttcggttt
     7441 caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct
     7501 cgctgaattc cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc
     7561 gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc
     7621 cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca
     7681 ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt
     7741 caggcttttt catatctcat tgcccgggat ctgcggcacg ctgttgacgc tgttaagcgg
     7801 gtcgctgcag ggtcgctcgg tgttcgaggc cacacgcgtc accttaatat gcgaagtgga
     7861 cctgggaccg cgccgccccg actgcatctg cgtgttcgaa ttcgccaatg acaagacgct
     7921 gggcggggtt tgtgtcatca tagaactaaa gacatgcaaa tatatttctt ccggggacac
     7981 cgccagcaaa cgcgagcaac gggccacggg gatgaagcag ggcatggcgg ccgacgcgct
     8041 gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct
     8101 tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga
     8161 tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat
     8221 cactggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt
     8281 ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc
     8341 atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc
     8401 actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct
     8461 tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg cagcaaaagg
     8521 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     8581 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8641 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8701 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8761 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8821 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8881 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8941 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     9001 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     9061 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     9121 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9181 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     9241 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9301 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9361 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9421 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9481 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9541 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9601 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     9661 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     9721 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9781 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9841 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     9901 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     9961 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    10021 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    10081 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    10141 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    10201 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    10261 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
    10321 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag
    10381 aa
//
