LOCUS       40924_10391       10188 bp DNA     circular SYN 28-JAN-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10188)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 10188)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10188
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..587
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     polyA_signal    complement(672..806)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1531..3320)
                     /direction=LEFT
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(3625..5547)
                     /codon_start=1
                     /label=EBNA1
                     /note="Epstein-Barr nuclear antigen 1, also known as
                     EBNA-1"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     promoter        6068..6172
                     /label=AmpR promoter
     CDS             6173..7030
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7204..7792
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        8223..8368
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             8411..9445
                     /codon_start=1
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     polyA_signal    9490..9538
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatctc tagaagctgg gtaccagctg ctagcaagct tgctagcggc cgctcgaggc
      661 cggcaaggcc ggatccagac atgataagat acattgatga gtttggacaa accacaacta
      721 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
      781 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
      841 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
      901 ctgattatga tccggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
      961 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     1021 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaggt cgactctaga
     1081 ggatcgatcc ccgccccgga cgaactaaac ctgactacga catctctgcc ccttcttcgc
     1141 ggggcagtgc atgtaatccc ttcagttggt tggtacaact tgccaactgg gccctgttcc
     1201 acatgtgaca cgggggggga ccaaacacaa aggggttctc tgactgtagt tgacatcctt
     1261 ataaatggat gtgcacattt gccaacactg agtggctttc atcctggagc agactttgca
     1321 gtctgtggac tgcaacacaa cattgccttt atgtgtaact cttggctgaa gctcttacac
     1381 caatgctggg ggacatgtac ctcccagggg cccaggaaga ctacgggagg ctacaccaac
     1441 gtcaatcaga ggggcctgtg tagctaccga taagcggacc ctcaagaggg cattagcaat
     1501 agtgtttata aggccccctt gttaacccta aacgggtagc atatgcttcc cgggtagtag
     1561 tatatactat ccagactaac cctaattcaa tagcatatgt tacccaacgg gaagcatatg
     1621 ctatcgaatt agggttagta aaagggtcct aaggaacagc gatatctccc accccatgag
     1681 ctgtcacggt tttatttaca tggggtcagg attccacgag ggtagtgaac cattttagtc
     1741 acaagggcag tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc
     1801 ttcttcattc tccttcgttt agctaataga ataactgctg agttgtgaac agtaaggtgt
     1861 atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc agaataaaat ttggacgggg
     1921 ggttcagtgg tggcattgtg ctatgacacc aatataaccc tcacaaaccc cttgggcaat
     1981 aaatactagt gtaggaatga aacattctga atatctttaa caatagaaat ccatggggtg
     2041 gggacaagcc gtaaagactg gatgtccatc tcacacgaat ttatggctat gggcaacaca
     2101 taatcctagt gcaatatgat actggggtta ttaagatgtg tcccaggcag ggaccaagac
     2161 aggtgaacca tgttgttaca ctctatttgt aacaagggga aagagagtgg acgccgacag
     2221 cagcggactc cactggttgt ctctaacacc cccgaaaatt aaacggggct ccacgccaat
     2281 ggggcccata aacaaagaca agtggccact cttttttttg aaattgtgga gtgggggcac
     2341 gcgtcagccc ccacacgccg ccctgcggtt ttggactgta aaataagggt gtaataactt
     2401 ggctgattgt aaccccgcta accactgcgg tcaaaccact tgcccacaaa accactaatg
     2461 gcaccccggg gaatacctgc ataagtaggt gggcgggcca agataggggc gcgattgctg
     2521 cgatctggag gacaaattac acacacttgc gcctgagcgc caagcacagg gttgttggtc
     2581 ctcatattca cgaggtcgct gagagcacgg tgggctaatg ttgccatggg tagcatatac
     2641 tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc ataggctatc
     2701 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     2761 ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     2821 ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc
     2881 ctaatagaga ttagggtagt atatgctatc ctaatttata tctgggtagc atatactacc
     2941 caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatat gctatcctaa
     3001 tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa
     3061 tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa
     3121 tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa
     3181 tctgtatccg ggtagcatat gctatcctca tgcatataca gtcagcatat gatacccagt
     3241 agtagagtgg gagtgctatc ctttgcatat gccgccacct cccaaggggg cgtgaatttt
     3301 cgctgcttgt ccttttcctg ctggttgctc ccattcttag gtgaatttaa ggaggccagg
     3361 ctaaagccgt cgcatgtctg attgctcacc aggtaaatgt cgctaatgtt ttccaacgcg
     3421 agaaggtgtt gagcgcggag ctgagtgacg tgacaacatg ggtatgccca attgccccat
     3481 gttgggagga cgaaaatggt gacaagacag atggccagaa atacaccaac agcacgcatg
     3541 atgtctactg gggatttatt ctttagtgcg ggggaataca cggcttttaa tacgattgag
     3601 ggcgtctcct aacaagttac atcactcctg cccttcctca ccctcatctc catcacctcc
     3661 ttcatctccg tcatctccgt catcaccctc cgcggcagcc ccttccacca taggtggaaa
     3721 ccagggaggc aaatctactc catcgtcaaa gctgcacaca gtcaccctga tattgcaggt
     3781 aggagcgggc tttgtcataa caaggtcctt aatcgcatcc ttcaaaacct cagcaaatat
     3841 atgagtttgt aaaaagacca tgaaataaca gacaatggac tcccttagcg ggccaggttg
     3901 tgggccgggt ccaggggcca ttccaaaggg gagacgactc aatggtgtaa gacgacattg
     3961 tggaatagca agggcagttc ctcgccttag gttgtaaagg gaggtcttac tacctccata
     4021 tacgaacaca ccggcgaccc aagttccttc gtcggtagtc ctttctacgt gactcctagc
     4081 caggagagct cttaaacctt ctgcaatgtt ctcaaatttc gggttggaac ctccttgacc
     4141 acgatgcttt ccaaaccacc ctcctttttt gcgcctgcct ccatcaccct gaccccgggg
     4201 tccagtgctt gggccttctc ctgggtcatc tgcggggccc tgctctatcg ctcccggggg
     4261 cacgtcaggc tcaccatctg ggccaccttc ttggtggtat tcaaaataat cggcttcccc
     4321 tacagggtgg aaaaatggcc ttctacctgg agggggcctg cgcggtggag acccggatga
     4381 tgatgactga ctactgggac tcctgggcct cttttctcca cgtccacgac ctctccccct
     4441 ggctctttca cgacttcccc ccctggctct ttcacgtcct ctaccccggc ggcctccact
     4501 acctcctcga ccccggcctc cactacctcc tcgaccccgg cctccactgc ctcctcgacc
     4561 ccggcctcca cctcctgctc ctgcccctcc tgctcctgcc cctcctcctg ctcctgcccc
     4621 tcctgcccct cctgctcctg cccctcctgc ccctcctgct cctgcccctc ctgcccctcc
     4681 tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctcctgctcc
     4741 tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc
     4801 ccctcctgct cctgcccctc ctgctcctgc ccctcctgct cctgcccctc ctgctcctgc
     4861 ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgctcctg cccctcctgc
     4921 ccctcctgcc cctcctgctc ctgcccctcc tcctgctcct gcccctcctg cccctcctgc
     4981 ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc
     5041 tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctcctgc
     5101 tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc
     5161 ccctcctcct gctcctgccc ctcctgcccc tcctgcccct cctgcccctc ctcctgctcc
     5221 tgcccctcct cctgctcctg cccctcctgc tcctgcccct cccgctcctg ctcctgctcc
     5281 tgttccaccg tgggtccctt tgcagccaat gcaacttgga cgtttttggg gtctccggac
     5341 accatctcta tgtcttggcc ctgatcctga gccgcccggg gctcctggtc ttccgcctcc
     5401 tcgtcctcgt cctcttcccc gtcctcgtcc atggttatca ccccctcttc tttgaggtcc
     5461 actgccgccg gagccttctg gtccagatgt gtctcccttc tctcctaggc catttccagg
     5521 tcctgtacct ggcccctcgt cagacatgat tcacactaaa agagatcaat agacatcttt
     5581 attagacgac gctcagtgaa tacagggagt gcagactcct gccccctcca acagcccccc
     5641 caccctcatc cccttcatgg tcgctgtcag acagatccag gtctgaaaat tccccatcct
     5701 ccgaaccatc ctcgtcctca tcaccaatta ctcgcagccc ggaaaactcc cgctgaacat
     5761 cctcaagatt tgcgtcctga gcctcaagcc aggcctcaaa ttcctcgtcc ccctttttgc
     5821 tggacggtag ggatggggat tctcgggacc cctcctcttc ctcttcaagg tcaccagaca
     5881 gagatgctac tggggcaacg gaagaaaagc tgggtgcggc ctgtgaggat cagcttatcg
     5941 atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt
     6001 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     6061 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6121 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6181 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6241 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6301 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6361 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     6421 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6481 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6541 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6601 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6661 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc
     6721 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6781 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6841 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6901 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     6961 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     7021 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7081 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7141 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7201 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7261 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7321 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     7381 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7441 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7501 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7561 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7621 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7681 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7741 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7801 caacgcggcc tttttacggt tcctggcctt ttgctggcct tgaagctgtc cctgatggtc
     7861 gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc
     7921 gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta
     7981 gcccagcgcg tcggccccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg
     8041 gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct
     8101 ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ctgcttcatc cccgtggccc
     8161 gttgctcgcg tttgctggcg gtgtccccgg aagaaatata tttgcatgtc tttagttcta
     8221 tgatgacaca aaccccgccc agcgtcttgt cattggcgaa ttcgaacacg cagatgcagt
     8281 cggggcggcg cggtccgagg tccacttcgc atattaaggt gacgcgtgtg gcctcgaaca
     8341 ccgagcgacc ctgcagcgac ccgcttaaca gcgtcaacag cgtgccgcag atcccggggg
     8401 gcaatgagat atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
     8461 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
     8521 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
     8581 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
     8641 gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
     8701 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
     8761 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
     8821 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
     8881 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
     8941 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
     9001 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
     9061 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
     9121 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
     9181 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
     9241 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
     9301 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
     9361 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
     9421 ggatcgggag atgggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
     9481 gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcat
     9541 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
     9601 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
     9661 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagccactgg ccccgtgggt
     9721 tagggacggg gtcccccatg gggaatggtt tatggttcgt gggggttatt attttgggcg
     9781 ttgcgtgggg tcaggtccac gactggactg agcagacaga cccatggttt ttggatggcc
     9841 tgggcatgga ccgcatgtac tggcgcgaca cgaacaccgg gcgtctgtgg ctgccaaaca
     9901 cccccgaccc ccaaaaacca ccgcgcggat ttctggcgtg ccaagctagt cgaccaattc
     9961 tcatgtttga cagcttatca tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc
    10021 agaactggta ggtatggaag atctatacat tgaatcaata ttggcaatta gccatattag
    10081 tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat
    10141 atcataatat gtacatttat attggctcat gtccaatatg accgccat
//
