LOCUS       V013354                11625 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013354
VERSION     V013354
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11625)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11625
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..587
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             664..966
                     /gene="tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate ARV2/SF2). Accession#: P04614"
     polyA_signal    complement(976..1110)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     polyA_signal    complement(2111..2245)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2968..4757)
                     /direction=LEFT
                     /label="oriP"
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5062..6984)
                     /label="EBNA1"
                     /note="Epstein-Barr nuclear antigen 1, also known as
                     EBNA-1"
     promoter        7505..7609
                     /label="AmpR promoter"
     CDS             7610..8467
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8641..9229
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        9660..9805
                     /label="HSV TK promoter"
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9848..10882
                     /label="HygR"
                     /note="hygromycin B phosphotransferase"
     polyA_signal    10927..10975
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatctc tagaagctgg gtaccagctg ctagcaagct tgctagcggc cgctcgagcc
      661 accatggagc cagtagatcc taatctagag ccctggaagc atccaggaag tcagcctagg
      721 actgcttgta acaattgcta ttgtaaaaag tgttgctttc attgctacgc gtgtttcaca
      781 agaaaaggct taggcatctc ctatggcagg aagaagcgga gacagcgacg aagagctcct
      841 caggacagtc agactcatca agcttctcta tcaaagcaac ccgcctccca gtcccgaggg
      901 gacccgacag ggcccacgga atcgaagaag aaggtggaga gagagacaga gacagatccg
      961 ttcgattagc atgcagatcc agacatgata agatacattg atgagtttgg acaaaccaca
     1021 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     1081 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     1141 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     1201 atggctgatt atgatccggc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
     1261 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     1321 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg aggtcgactc
     1381 tagaggatcg atccccgccc cggacgaact aaacctgact acgacatctc tgccccttct
     1441 tcgcggggca gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgggccctg
     1501 ttccacatgt gacacggggg gggaccaaac acaaaggggt tctctgactg tagttgacat
     1561 ccttataaat ggatgtgcac atttgccaac actgagtggc tttcatcctg gagcagactt
     1621 tgcagtctgt ggactgcaac acaacattgc ctttatgtgt aactcttggc tgaagctctt
     1681 acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg gaggctacac
     1741 caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag agggcattag
     1801 caatagtgtt tataaggccc ccttgttaac cctaaacggg tagcatatgc ttcccgggta
     1861 gtagtatata ctatccagac taaccctaat tcaatagcat atgttaccca acgggaagca
     1921 tatgctatcg aattagggtt agtaaaaggg tcctaaggaa cagcgatatc tcccacccca
     1981 tgagctgtca cggttttatt tacatggggt caggattcca cgagggtagt gaaccatttt
     2041 agtcacaagg gcagtggctg aagatcaagg agcgggcagg gaatctcctg aatctcggcc
     2101 ggcaaggccg gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     2161 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     2221 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     2281 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     2341 tgattatgat ccggctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     2401 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     2461 caggcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgaggtcgac tctagaggat
     2521 cgatgccccg ccccggacga actaaacctg actacgacat ctctgcccct tcttcgcggg
     2581 gcagtgcatg taatcccttc agttggttgg tacaacttgc caactgggcc ctgttccaca
     2641 tgtgacacgg ggggggacca aacacaaagg ggttctctga ctgtagttga catccttata
     2701 aatggatgtg cacatttgcc aacactgagt ggctttcatc ctggagcaga ctttgcagtc
     2761 tgtggactgc aacacaacat tgcctttatg tgtaactctt ggctgaagct cttacaccaa
     2821 tgctggggga catgtacctc ccaggggccc aggaagacta cgggaggcta caccaacgtc
     2881 aatcagaggg gcctgtgtag ctaccgataa gcggaccctc aagagggcat tagcaatagt
     2941 gtttataagg cccccttgtt aaccctaaac gggtagcata tgcttcccgg gtagtagtat
     3001 atactatcca gactaaccct aattcaatag catatgttac ccaacgggaa gcatatgcta
     3061 tcgaattagg gttagtaaaa gggtcctaag gaacagcgat atctcccacc ccatgagctg
     3121 tcacggtttt atttacatgg ggtcaggatt ccacgagggt agtgaaccat tttagtcaca
     3181 agggcagtgg ctgaagatca aggagcgggc agtgaactct cctgaatctt cgcctgcttc
     3241 ttcattctcc ttcgtttagc taatagaata actgctgagt tgtgaacagt aaggtgtatg
     3301 tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga ataaaatttg gacggggggt
     3361 tcagtggtgg cattgtgcta tgacaccaat ataaccctca caaacccctt gggcaataaa
     3421 tactagtgta ggaatgaaac attctgaata tctttaacaa tagaaatcca tggggtgggg
     3481 acaagccgta aagactggat gtccatctca cacgaattta tggctatggg caacacataa
     3541 tcctagtgca atatgatact ggggttatta agatgtgtcc caggcaggga ccaagacagg
     3601 tgaaccatgt tgttacactc tatttgtaac aaggggaaag agagtggacg ccgacagcag
     3661 cggactccac tggttgtctc taacaccccc gaaaattaaa cggggctcca cgccaatggg
     3721 gcccataaac aaagacaagt ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg
     3781 tcagccccca cacgccgccc tgcggttttg gactgtaaaa taagggtgta ataacttggc
     3841 tgattgtaac cccgctaacc actgcggtca aaccacttgc ccacaaaacc actaatggca
     3901 ccccggggaa tacctgcata agtaggtggg cgggccaaga taggggcgcg attgctgcga
     3961 tctggaggac aaattacaca cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc
     4021 atattcacga ggtcgctgag agcacggtgg gctaatgttg ccatgggtag catatactac
     4081 ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata ggctatccta
     4141 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     4201 atttatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     4261 atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata tgctatccta
     4321 atagagatta gggtagtata tgctatccta atttatatct gggtagcata tactacccaa
     4381 atatctggat agcatatgct atcctaatct atatctgggt agcatatgct atcctaatct
     4441 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     4501 atatctgggt agtatatgct atcctaattt atatctgggt agcataggct atcctaatct
     4561 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct
     4621 gtatccgggt agcatatgct atcctcatgc atatacagtc agcatatgat acccagtagt
     4681 agagtgggag tgctatcctt tgcatatgcc gccacctccc aagggggcgt gaattttcgc
     4741 tgcttgtcct tttcctgctg gttgctccca ttcttaggtg aatttaagga ggccaggcta
     4801 aagccgtcgc atgtctgatt gctcaccagg taaatgtcgc taatgttttc caacgcgaga
     4861 aggtgttgag cgcggagctg agtgacgtga caacatgggt atgcccaatt gccccatgtt
     4921 gggaggacga aaatggtgac aagacagatg gccagaaata caccaacagc acgcatgatg
     4981 tctactgggg atttattctt tagtgcgggg gaatacacgg cttttaatac gattgagggc
     5041 gtctcctaac aagttacatc actcctgccc ttcctcaccc tcatctccat cacctccttc
     5101 atctccgtca tctccgtcat caccctccgc ggcagcccct tccaccatag gtggaaacca
     5161 gggaggcaaa tctactccat cgtcaaagct gcacacagtc accctgatat tgcaggtagg
     5221 agcgggcttt gtcataacaa ggtccttaat cgcatccttc aaaacctcag caaatatatg
     5281 agtttgtaaa aagaccatga aataacagac aatggactcc cttagcgggc caggttgtgg
     5341 gccgggtcca ggggccattc caaaggggag acgactcaat ggtgtaagac gacattgtgg
     5401 aatagcaagg gcagttcctc gccttaggtt gtaaagggag gtcttactac ctccatatac
     5461 gaacacaccg gcgacccaag ttccttcgtc ggtagtcctt tctacgtgac tcctagccag
     5521 gagagctctt aaaccttctg caatgttctc aaatttcggg ttggaacctc cttgaccacg
     5581 atgctttcca aaccaccctc cttttttgcg cctgcctcca tcaccctgac cccggggtcc
     5641 agtgcttggg ccttctcctg ggtcatctgc ggggccctgc tctatcgctc ccgggggcac
     5701 gtcaggctca ccatctgggc caccttcttg gtggtattca aaataatcgg cttcccctac
     5761 agggtggaaa aatggccttc tacctggagg gggcctgcgc ggtggagacc cggatgatga
     5821 tgactgacta ctgggactcc tgggcctctt ttctccacgt ccacgacctc tccccctggc
     5881 tctttcacga cttccccccc tggctctttc acgtcctcta ccccggcggc ctccactacc
     5941 tcctcgaccc cggcctccac tacctcctcg accccggcct ccactgcctc ctcgaccccg
     6001 gcctccacct cctgctcctg cccctcctgc tcctgcccct cctcctgctc ctgcccctcc
     6061 tgcccctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
     6121 tcctgcccct cctgcccctc ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc
     6181 ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc
     6241 tcctgctcct gcccctcctg ctcctgcccc tcctgctcct gcccctcctg ctcctgcccc
     6301 tcctgcccct cctgcccctc ctcctgctcc tgcccctcct gctcctgccc ctcctgcccc
     6361 tcctgcccct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc
     6421 tcctcctgct cctgcccctc ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc
     6481 ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctcctgctcc
     6541 tgcccctcct cctgctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc
     6601 tcctcctgct cctgcccctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc
     6661 ccctcctcct gctcctgccc ctcctgctcc tgcccctccc gctcctgctc ctgctcctgt
     6721 tccaccgtgg gtccctttgc agccaatgca acttggacgt ttttggggtc tccggacacc
     6781 atctctatgt cttggccctg atcctgagcc gcccggggct cctggtcttc cgcctcctcg
     6841 tcctcgtcct cttccccgtc ctcgtccatg gttatcaccc cctcttcttt gaggtccact
     6901 gccgccggag ccttctggtc cagatgtgtc tcccttctct cctaggccat ttccaggtcc
     6961 tgtacctggc ccctcgtcag acatgattca cactaaaaga gatcaataga catctttatt
     7021 agacgacgct cagtgaatac agggagtgca gactcctgcc ccctccaaca gcccccccac
     7081 cctcatcccc ttcatggtcg ctgtcagaca gatccaggtc tgaaaattcc ccatcctccg
     7141 aaccatcctc gtcctcatca ccaattactc gcagcccgga aaactcccgc tgaacatcct
     7201 caagatttgc gtcctgagcc tcaagccagg cctcaaattc ctcgtccccc tttttgctgg
     7261 acggtaggga tggggattct cgggacccct cctcttcctc ttcaaggtca ccagacagag
     7321 atgctactgg ggcaacggaa gaaaagctgg gtgcggcctg tgaggatcag cttatcgatg
     7381 ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt
     7441 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     7501 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7561 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     7621 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7681 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     7741 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     7801 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
     7861 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     7921 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     7981 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     8041 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8101 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat
     8161 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8221 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8281 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     8341 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8401 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8461 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     8521 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8581 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8641 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     8701 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     8761 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     8821 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8881 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8941 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     9001 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     9061 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     9121 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9181 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9241 cgcggccttt ttacggttcc tggccttttg ctggccttga agctgtccct gatggtcgtc
     9301 atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag
     9361 aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc
     9421 cagcgcgtcg gccccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat
     9481 atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca
     9541 attcttggag tggtgaatcc gttagcgagg tgccgccctg cttcatcccc gtggcccgtt
     9601 gctcgcgttt gctggcggtg tccccggaag aaatatattt gcatgtcttt agttctatga
     9661 tgacacaaac cccgcccagc gtcttgtcat tggcgaattc gaacacgcag atgcagtcgg
     9721 ggcggcgcgg tccgaggtcc acttcgcata ttaaggtgac gcgtgtggcc tcgaacaccg
     9781 agcgaccctg cagcgacccg cttaacagcg tcaacagcgt gccgcagatc ccggggggca
     9841 atgagatatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa
     9901 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag
     9961 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta
    10021 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct
    10081 tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt
    10141 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc
    10201 catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc
    10261 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca
    10321 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct
    10381 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga
    10441 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag
    10501 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg
    10561 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg
    10621 atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt
    10681 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg
    10741 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac
    10801 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgga
    10861 tcgggagatg ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
    10921 ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa
    10981 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
    11041 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
    11101 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag ccactggccc cgtgggttag
    11161 ggacggggtc ccccatgggg aatggtttat ggttcgtggg ggttattatt ttgggcgttg
    11221 cgtggggtca ggtccacgac tggactgagc agacagaccc atggtttttg gatggcctgg
    11281 gcatggaccg catgtactgg cgcgacacga acaccgggcg tctgtggctg ccaaacaccc
    11341 ccgaccccca aaaaccaccg cgcggatttc tggcgtgcca agctagtcga ccaattctca
    11401 tgtttgacag cttatcatcg cagatccggg caacgttgtt gccattgctg caggcgcaga
    11461 actggtaggt atggaagatc tatacattga atcaatattg gcaattagcc atattagtca
    11521 ttggttatat agcataaatc aatattggct attggccatt gcatacgttg tatctatatc
    11581 ataatatgta catttatatt ggctcatgtc caatatgacc gccat
//
