LOCUS       40924_10381       10209 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 10209)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..587
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             635..658
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     polyA_signal    complement(695..829)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1552..3341)
                     /direction=LEFT
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(3646..5568)
                     /codon_start=1
                     /label=EBNA1
                     /note="Epstein-Barr nuclear antigen 1, also known as
                     EBNA-1"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     promoter        6089..6193
                     /label=AmpR promoter
     CDS             6194..7051
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7225..7813
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        8244..8389
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             8432..9466
                     /codon_start=1
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     polyA_signal    9511..9559
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatctc tagaagctgg gtaccgccac catggattac aaggatgacg acgataagaa
      661 gcttgctagc ggccgctcga ggccggcaag gccggatcca gacatgataa gatacattga
      721 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
      781 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
      841 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
      901 aaacctctac aaatgtggta tggctgatta tgatccggct gcctcgcgcg tttcggtgat
      961 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     1021 gatgccggga gcagacaagc ccgtcaggcg tcagcgggtg ttggcgggtg tcggggcgca
     1081 gccatgaggt cgactctaga ggatcgatgc cccgccccgg acgaactaaa cctgactacg
     1141 acatctctgc cccttcttcg cggggcagtg catgtaatcc cttcagttgg ttggtacaac
     1201 ttgccaactg ggccctgttc cacatgtgac acgggggggg accaaacaca aaggggttct
     1261 ctgactgtag ttgacatcct tataaatgga tgtgcacatt tgccaacact gagtggcttt
     1321 catcctggag cagactttgc agtctgtgga ctgcaacaca acattgcctt tatgtgtaac
     1381 tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg gcccaggaag
     1441 actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg ataagcggac
     1501 cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct aaacgggtag
     1561 catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca atagcatatg
     1621 ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc taaggaacag
     1681 cgatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag gattccacga
     1741 gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc gggcagtgaa
     1801 ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag aataactgct
     1861 gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc
     1921 cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac caatataacc
     1981 ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg aatatcttta
     2041 acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat ctcacacgaa
     2101 tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt attaagatgt
     2161 gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg taacaagggg
     2221 aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac ccccgaaaat
     2281 taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac tctttttttt
     2341 gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt tttggactgt
     2401 aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg gtcaaaccac
     2461 ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg tgggcgggcc
     2521 aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg cgcctgagcg
     2581 ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg gtgggctaat
     2641 gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
     2701 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     2761 atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat
     2821 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt
     2881 atccgggtag catatgctat cctaatagag attagggtag tatatgctat cctaatttat
     2941 atctgggtag catatactac ccaaatatct ggatagcata tgctatccta atctatatct
     3001 gggtagcata tgctatccta atctatatct gggtagcata ggctatccta atctatatct
     3061 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atttatatct
     3121 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     3181 gggtagtata tgctatccta atctgtatcc gggtagcata tgctatcctc atgcatatac
     3241 agtcagcata tgatacccag tagtagagtg ggagtgctat cctttgcata tgccgccacc
     3301 tcccaagggg gcgtgaattt tcgctgcttg tccttttcct gctggttgct cccattctta
     3361 ggtgaattta aggaggccag gctaaagccg tcgcatgtct gattgctcac caggtaaatg
     3421 tcgctaatgt tttccaacgc gagaaggtgt tgagcgcgga gctgagtgac gtgacaacat
     3481 gggtatgccc aattgcccca tgttgggagg acgaaaatgg tgacaagaca gatggccaga
     3541 aatacaccaa cagcacgcat gatgtctact ggggatttat tctttagtgc gggggaatac
     3601 acggctttta atacgattga gggcgtctcc taacaagtta catcactcct gcccttcctc
     3661 accctcatct ccatcacctc cttcatctcc gtcatctccg tcatcaccct ccgcggcagc
     3721 cccttccacc ataggtggaa accagggagg caaatctact ccatcgtcaa agctgcacac
     3781 agtcaccctg atattgcagg taggagcggg ctttgtcata acaaggtcct taatcgcatc
     3841 cttcaaaacc tcagcaaata tatgagtttg taaaaagacc atgaaataac agacaatgga
     3901 ctcccttagc gggccaggtt gtgggccggg tccaggggcc attccaaagg ggagacgact
     3961 caatggtgta agacgacatt gtggaatagc aagggcagtt cctcgcctta ggttgtaaag
     4021 ggaggtctta ctacctccat atacgaacac accggcgacc caagttcctt cgtcggtagt
     4081 cctttctacg tgactcctag ccaggagagc tcttaaacct tctgcaatgt tctcaaattt
     4141 cgggttggaa cctccttgac cacgatgctt tccaaaccac cctccttttt tgcgcctgcc
     4201 tccatcaccc tgaccccggg gtccagtgct tgggccttct cctgggtcat ctgcggggcc
     4261 ctgctctatc gctcccgggg gcacgtcagg ctcaccatct gggccacctt cttggtggta
     4321 ttcaaaataa tcggcttccc ctacagggtg gaaaaatggc cttctacctg gagggggcct
     4381 gcgcggtgga gacccggatg atgatgactg actactggga ctcctgggcc tcttttctcc
     4441 acgtccacga cctctccccc tggctctttc acgacttccc cccctggctc tttcacgtcc
     4501 tctaccccgg cggcctccac tacctcctcg accccggcct ccactacctc ctcgaccccg
     4561 gcctccactg cctcctcgac cccggcctcc acctcctgct cctgcccctc ctgctcctgc
     4621 ccctcctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
     4681 tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc
     4741 tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc
     4801 tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgctcctg cccctcctgc
     4861 tcctgcccct cctgctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc
     4921 tcctgctcct gcccctcctg cccctcctgc ccctcctgct cctgcccctc ctcctgctcc
     4981 tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctcctgc
     5041 tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc
     5101 ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc
     5161 tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc
     5221 tcctgcccct cctcctgctc ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc
     5281 tcccgctcct gctcctgctc ctgttccacc gtgggtccct ttgcagccaa tgcaacttgg
     5341 acgtttttgg ggtctccgga caccatctct atgtcttggc cctgatcctg agccgcccgg
     5401 ggctcctggt cttccgcctc ctcgtcctcg tcctcttccc cgtcctcgtc catggttatc
     5461 accccctctt ctttgaggtc cactgccgcc ggagccttct ggtccagatg tgtctccctt
     5521 ctctcctagg ccatttccag gtcctgtacc tggcccctcg tcagacatga ttcacactaa
     5581 aagagatcaa tagacatctt tattagacga cgctcagtga atacagggag tgcagactcc
     5641 tgccccctcc aacagccccc ccaccctcat ccccttcatg gtcgctgtca gacagatcca
     5701 ggtctgaaaa ttccccatcc tccgaaccat cctcgtcctc atcaccaatt actcgcagcc
     5761 cggaaaactc ccgctgaaca tcctcaagat ttgcgtcctg agcctcaagc caggcctcaa
     5821 attcctcgtc cccctttttg ctggacggta gggatgggga ttctcgggac ccctcctctt
     5881 cctcttcaag gtcaccagac agagatgcta ctggggcaac ggaagaaaag ctgggtgcgg
     5941 cctgtgagga tcagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa
     6001 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     6061 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     6121 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     6181 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     6241 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     6301 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     6361 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     6421 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     6481 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6541 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6601 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6661 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6721 tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6781 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6841 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6901 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6961 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     7021 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     7081 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     7141 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     7201 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     7261 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7321 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     7381 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7441 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7501 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7561 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7621 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7681 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7741 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7801 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7861 ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg
     7921 catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt
     7981 cgcgaacgcc agcaagacgt agcccagcgc gtcggccccg agatgcgccg cgtgcggctg
     8041 ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt
     8101 ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc
     8161 cctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat
     8221 atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga
     8281 attcgaacac gcagatgcag tcggggcggc gcggtccgag gtccacttcg catattaagg
     8341 tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaac agcgtcaaca
     8401 gcgtgccgca gatcccgggg ggcaatgaga tatgaaaaag cctgaactca ccgcgacgtc
     8461 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     8521 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     8581 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     8641 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
     8701 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     8761 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
     8821 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     8881 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     8941 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     9001 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     9061 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     9121 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     9181 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     9241 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     9301 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     9361 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     9421 ccgacgcccc agcactcgtc cggatcggga gatgggggag gctaactgaa acacggaagg
     9481 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     9541 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     9601 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
     9661 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
     9721 atagccactg gccccgtggg ttagggacgg ggtcccccat ggggaatggt ttatggttcg
     9781 tgggggttat tattttgggc gttgcgtggg gtcaggtcca cgactggact gagcagacag
     9841 acccatggtt tttggatggc ctgggcatgg accgcatgta ctggcgcgac acgaacaccg
     9901 ggcgtctgtg gctgccaaac acccccgacc cccaaaaacc accgcgcgga tttctggcgt
     9961 gccaagctag tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt
    10021 tgttgccatt gctgcaggcg cagaactggt aggtatggaa gatctataca ttgaatcaat
    10081 attggcaatt agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc
    10141 cattgcatac gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat
    10201 gaccgccat
//
