LOCUS       V008575                 6920 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008575
VERSION     V008575
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6920)
  AUTHORS   Merighi M, Ellermeier CD, Slauch JM, Gunn JS.
  TITLE     Resolvase-IVET Analysis of the Salmonella enterica sv. typhimurium
            PhoP and PmrA regulons in BALB/c mice
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6920)
  AUTHORS   Smith JN, Ellermeier CD, Dyszel J, Altier C, Young GM, Lawhon SD,
            Adams LG, Konjufca VK, Curtiss R III, Slauch JM, Ahmer BMM.
  TITLE     Recording of SdiA activity during transit of Salmonella through
            animals
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6920)
  AUTHORS   Ellermeier CD, Slauch JM.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2005) Microbiology, University of Illinois
            Urbana-Champaign, 601 S. Goodwin Ave. B103 CLSL, Urbana, IL 61801,
            USA
REFERENCE   4  (bases 1 to 6920)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6920)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (23-MAY-2005) Microbiology, University of Illinois Urbana-Champaign,
            601 S. Goodwin Ave. B103 CLSL, Urbana, IL 61801, USA"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(40..73)
                     /label="FRT (minimal)"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     regulatory      82..89
                     /gene="tnpR"
                     /regulatory_class="ribosome_binding_site"
     CDS             99..647
                     /gene="tnpR"
                     /label="Transposon gamma-delta resolvase"
                     /note="Transposon gamma-delta resolvase from Escherichia
                     coli (strain K12). Accession#: P03012"
     CDS             675..3746
                     /label="lacZ"
                     /note="beta-galactosidase"
     CDS             3801..5051
                     /gene="lacY"
                     /label="Lactose permease"
                     /note="Lactose permease from Escherichia coli (strain K12).
                     Accession#: P02920"
     regulatory      5260..5289
                     /note="histidine bi-directional rho-independent terminator
                     from Salmonella typhimurium"
                     /regulatory_class="terminator"
     CDS             complement(5540..6352)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      6522..6910
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 ccacttgtgt ataagagtca ggaagaagct tgggatcggg aagttcctat actttctaga
       61 gaataggaac ttcccgatct gatttaggat acatttttat gccacttttt ggttacgcac
      121 gggtatcaac cagccagcaa tctctcgata ttcaggttcg ggcactcaaa gacgcaggcg
      181 tgaaagcaaa tcgcatcttt actgacaagg catcgggcag ttcaagcgat ccgaaagggc
      241 tggacttgct gaggatgaag gtggaggaag gtgacgtcat cttggtgaag aaacttgacc
      301 gccttgggcg cgatactgct gacatgatcc agttaataaa agagtttgac gcccaaggtg
      361 tatccattcg gtttattgat gacggaatca gtaccgatgg ggagatgggt aaaatggttg
      421 tcactattct atctgcagtg gcccaggcag aacgacagag aatactagag cgtaccaatg
      481 aaggtcgcca agaggcaatg gcaaaaggag ttgtttttgg tagaaaaaga aaaatagata
      541 gagatgcagt attaaatatg tggcaacagg ggttaggtgc ctcacatata tcaaaaacaa
      601 tgaatattcc tcgttcaaca gtatataaag taataaatga aagcaactaa cataaaagca
      661 ggtaccaaac agctatgacc atgattacgg attcactggc cgtcgtttta caacgtcgtg
      721 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
      781 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
      841 atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa agctggctgg
      901 agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag atgcacggtt
      961 acgatgcgcc catctacacc aacgtaacct atcccattac ggtcaatccg ccgtttgttc
     1021 ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa agctggctac
     1081 aggaaggcca gacgcgaatt atttttgatg gcgttaactc ggcgtttcat ctgtggtgca
     1141 acgggcgctg ggtcggttac ggccaggaca gtcgtttgcc gtctgaattt gacctgagcg
     1201 catttttacg cgccggagaa aaccgcctcg cggtgatggt gctgcgttgg agtgacggca
     1261 gttatctgga agatcaggat atgtggcgga tgagcggcat tttccgtgac gtctcgttgc
     1321 tgcataaacc gactacacaa atcagcgatt tccatgttgc cactcgcttt aatgatgatt
     1381 tcagccgcgc tgtactggag gctgaagttc agatgtgcgg cgagttgcgt gactacctac
     1441 gggtaacagt ttctttatgg cagggtgaaa cgcaggtcgc cagcggcacc gcgcctttcg
     1501 gcggtgaaat tatcgatgag cgtggtggtt atgccgatcg cgtcacacta cgtctgaacg
     1561 tcgaaaaccc gaaactgtgg agcgccgaaa tcccgaatct ctatcgtgcg gtggttgaac
     1621 tgcacaccgc cgacggcacg ctgattgaag cagaagcctg cgatgtcggt ttccgcgagg
     1681 tgcggattga aaatggtctg ctgctgctga acggcaagcc gttgctgatt cgaggcgtta
     1741 accgtcacga gcatcatcct ctgcatggtc aggtcatgga tgagcagacg atggtgcagg
     1801 atatcctgct gatgaagcag aacaacttta acgccgtgcg ctgttcgcat tatccgaacc
     1861 atccgctgtg gtacacgctg tgcgaccgct acggcctgta tgtggtggat gaagccaata
     1921 ttgaaaccca cggcatggtg ccaatgaatc gtctgaccga tgatccgcgc tggctaccgg
     1981 cgatgagcga acgcgtaacg cgaatggtgc agcgcgatcg taatcacccg agtgtgatca
     2041 tctggtcgct ggggaatgaa tcaggccacg gcgctaatca cgacgcgctg tatcgctgga
     2101 tcaaatctgt cgatccttcc cgcccggtgc agtatgaagg cggcggagcc gacaccacgg
     2161 ccaccgatat tatttgcccg atgtacgcgc gcgtggatga agaccagccc ttcccggctg
     2221 tgccgaaatg gtccatcaaa aaatggcttt cgctacctgg agagacgcgc ccgctgatcc
     2281 tttgcgaata cgcccacgcg atgggtaaca gtcttggcgg tttcgctaaa tactggcagg
     2341 cgtttcgtca gtatccccgt ttacagggcg gcttcgtctg ggactgggtg gatcagtcgc
     2401 tgattaaata tgatgaaaac ggcaacccgt ggtcggctta cggcggtgat tttggcgata
     2461 cgccgaacga tcgccagttc tgtatgaacg gtctggtctt tgccgaccgc acgccgcatc
     2521 cagcgctgac ggaagcaaaa caccagcagc agtttttcca gttccgttta tccgggcaaa
     2581 ccatcgaagt gaccagcgaa tacctgttcc gtcatagcga taacgagctc ctgcactgga
     2641 tggtggcgct ggatggtaag ccgctggcaa gcggtgaagt gcctctggat gtcgctccac
     2701 aaggtaaaca gttgattgaa ctgcctgaac taccgcagcc ggagagcgcc gggcaactct
     2761 ggctcacagt acgcgtagtg caaccgaacg cgaccgcatg gtcagaagcc gggcacatca
     2821 gcgcctggca gcagtggcgt ctggcggaaa acctcagtgt gacgctcccc gccgcgtccc
     2881 acgccatccc gcatctgacc accagcgaaa tggatttttg catcgagctg ggtaataagc
     2941 gttggcaatt taaccgccag tcaggctttc tttcacagat gtggattggc gataaaaaac
     3001 aactgctgac gccgctgcgc gatcagttca cccgtgcacc gctggataac gacattggcg
     3061 taagtgaagc gacccgcatt gaccctaacg cctgggtcga acgctggaag gcggcgggcc
     3121 attaccaggc cgaagcagcg ttgttgcagt gcacggcaga tacacttgct gatgcggtgc
     3181 tgattacgac cgctcacgcg tggcagcatc aggggaaaac cttatttatc agccggaaaa
     3241 cctaccggat tgatggtagt ggtcaaatgg cgattaccgt tgatgttgaa gtggcgagcg
     3301 atacaccgca tccggcgcgg attggcctga actgccagct ggcgcaggta gcagagcggg
     3361 taaactggct cggattaggg ccgcaagaaa actatcccga ccgccttact gccgcctgtt
     3421 ttgaccgctg ggatctgcca ttgtcagaca tgtatacccc gtacgtcttc ccgagcgaaa
     3481 acggtctgcg ctgcgggacg cgcgaattga attatggccc acaccagtgg cgcggcgact
     3541 tccagttcaa catcagccgc tacagtcaac agcaactgat ggaaaccagc catcgccatc
     3601 tgctgcacgc ggaagaaggc acatggctga atatcgacgg tttccatatg gggattggtg
     3661 gcgacgactc ctggagcccg tcagtatcgg cggaattcca gctgagcgcc ggtcgctacc
     3721 attaccagtt ggtctggtgt caaaaataat aataaccggg caggccatgt ctgcccgtat
     3781 ttcgcgtaag gaaatccatt atgtactatt taaaaaacac aaacttttgg atgttcggtt
     3841 tattcttttt cttttacttt tttatcatgg gagcctactt cccgtttttc ccgatttggc
     3901 tacatgacat caaccatatc agcaaaagtg atacgggtat tatttttgcc gctatttctc
     3961 tgttctcgct attattccaa ccgctgtttg gtctgctttc tgacaaactc gggctgcgca
     4021 aatacctgct gtggattatt accggcatgt tagtgatgtt tgcgccgttc tttattttta
     4081 tcttcgggcc actgttacaa tacaacattt tagtaggatc gattgttggt ggtatttatc
     4141 taggcttttg ttttaacgcc ggtgcgccag cagtagaggc atttattgag aaagtcagcc
     4201 gtcgcagtaa tttcgaattt ggtcgcgcgc ggatgtttgg ctgtgttggc tgggcgctgt
     4261 gtgcctcgat tgtcggcatc atgttcacca tcaataatca gtttgttttc tggctgggct
     4321 ctggctgtgc actcatcctc gccgttttac tctttttcgc caaaacggat gcgccctctt
     4381 ctgccacggt tgccaatgcg gtaggtgcca accattcggc atttagcctt aagctggcac
     4441 tggaactgtt cagacagcca aaactgtggt ttttgtcact gtatgttatt ggcgtttcct
     4501 gcacctacga tgtttttgac caacagtttg ctaatttctt tacttcgttc tttgctaccg
     4561 gtgaacaggg tacgcgggta tttggctacg taacgacaat gggcgaatta cttaacgcct
     4621 cgattatgtt ctttgcgcca ctgatcatta atcgcatcgg tgggaaaaac gccctgctgc
     4681 tggctggcac tattatgtct gtacgtatta ttggctcatc gttcgccacc tcagcgctgg
     4741 aagtggttat tctgaaaacg ctgcatatgt ttgaagtacc gttcctgctg gtgggctgct
     4801 ttaaatatat taccagccag tttgaagtgc gtttttcagc gacgatttat ctggtctgtt
     4861 tctgcttctt taagcaactg gcgatgattt ttatgtctgt actggcgggc aatatgtatg
     4921 aaagcatcgg tttccagggc gcttatctgg tgctgggtct ggtggcgctg ggcttcacct
     4981 taatttccgt gttcacgctt agcggccccg gcccgctttc cctgctgcgt cgtcaggtga
     5041 atgaagtcgc ttaagcaatc aatgtcggat gcggcgcgac gcttatccga ccaacatatc
     5101 ataacggagt gatcgcattg aacatgccaa tgaccgaaag aataagagca ggcaagctat
     5161 ttaccgatat gtgcgaaggc ttaccggaaa aaagacttcg tgggaaaagc atgcgattta
     5221 agcaatcaat gtcggatgcg gcgcgacgct tatccgacca aaaaaaccgg gcaatgcccg
     5281 gtttttttta attattcctg aatcgcccca tcatccagcc agaaagtgag ggagccacgg
     5341 ttgatgagag ctttgttgta ggtggaccag ttggtgattt tgaacttttg ctttgccacg
     5401 gaacggtctg cgttgtcggg aagatgcgtg atctgatcct tcaactcagc aaaagttcga
     5461 tttattcaac aaagccgccg tcccgtcaag tcagcgtaat gctctgccag tgttacaacc
     5521 aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca
     5581 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
     5641 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc
     5701 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
     5761 caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga
     5821 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt
     5881 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat
     5941 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt
     6001 cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg
     6061 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa
     6121 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt
     6181 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg
     6241 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt
     6301 tggaatttaa tcgcggcctc gagcaagacg tttcccgttg aatatggctc ataacacccc
     6361 ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt
     6421 gtgcaatgta acatcagaga ttttgagaca caacgtggct ttcccccccc cccccgagct
     6481 catcgatttc gaaccccggc cacgatgcgt ccggcgtaga ggatctgaag atcagcagtt
     6541 caacctgttg atagtacgta ctaagctctc atgtttcacg tactaagctc tcatgtttaa
     6601 cgtactaagc tctcatgttt aacgaactaa accctcatgg ctaacgtact aagctctcat
     6661 ggctaacgta ctaagctctc atgtttcacg tactaagctc tcatgtttga acaataaaat
     6721 taatataaat cagcaactta aatagcctct aaggttttaa gttttataag aaaaaaaaga
     6781 atatataagg cttttaaagc ttttaaggtt taacggttgt ggacaacaag ccagggatgt
     6841 aacgcactga gaagccctta gagcctctca aagcaatttt gagtgacaca ggaacactta
     6901 acggctgaca tgggggggta
//
