LOCUS       V008576                 6274 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008576
VERSION     V008576
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6274)
  AUTHORS   Ellermeier CD, Janakiraman A, Slauch JM.
  TITLE     Construction of targeted single copy lac fusions using lambda Red
            and FLP-mediated site-specific recombination in bacteria
  JOURNAL   Gene 290 (1-2), 153-161 (2002)
   PUBMED   12062810
REFERENCE   2  (bases 1 to 6274)
  AUTHORS   Ellermeier CD, Janakiraman A, Slauch JM.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2001) Microbiology, University of Illinois
            Urbana-Champaign, 601 S. Goodwin Ave. B103 CLSL, Urbana, IL 61801,
            USA
REFERENCE   3  (bases 1 to 6274)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6274)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 290
            (1-2), 153-161 (2002)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-NOV-2001) Microbiology, University of Illinois Urbana-Champaign,
            601 S. Goodwin Ave. B103 CLSL, Urbana, IL 61801, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    122..155
                     /label="FRT (minimal)"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     CDS             160..3222
                     /label="lacZ"
                     /note="beta-galactosidase"
     CDS             3277..4527
                     /gene="lacY"
                     /label="Lactose permease"
                     /note="Lactose permease from Escherichia coli (strain K12).
                     Accession#: P02920"
     regulatory      4736..4765
                     /note="histidine bi-directional rho-independent terminator
                     from Salmonella typhimurium"
                     /regulatory_class="terminator"
     CDS             complement(5016..5828)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      5998..6274
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 gcttttaagg tttaacggtt gtggacaaca agccagggat gtaacgcact gagaagccct
       61 tagagcctct caaagcaatt ttgagtgaca caggaacact taacggctga catggggggg
      121 ggaagttcct attctctaga aagaatagga acttctagca ttacggattc actggccgtc
      181 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      241 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      301 cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg
      361 ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac
      421 tggcagatgc acggttacga tgcgcccatc tacaccaacg taacctatcc cattacggtc
      481 aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac atttaatgtt
      541 gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggcgt taactcggcg
      601 tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc aggacagtcg tttgccgtct
      661 gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg
      721 cgttggagtg acggcagtta tctggaagat caggatatgt ggcggatgag cggcattttc
      781 cgtgacgtct cgttgctgca taaaccgact acacaaatca gcgatttcca tgttgccact
      841 cgctttaatg atgatttcag ccgcgctgta ctggaggctg aagttcagat gtgcggcgag
      901 ttgcgtgact acctacgggt aacagtttct ttatggcagg gtgaaacgca ggtcgccagc
      961 ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc
     1021 acactacgtc tgaacgtcga aaacccgaaa ctgtggagcg ccgaaatccc gaatctctat
     1081 cgtgcggtgg ttgaactgca caccgccgac ggcacgctga ttgaagcaga agcctgcgat
     1141 gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg caagccgttg
     1201 ctgattcgag gcgttaaccg tcacgagcat catcctctgc atggtcaggt catggatgag
     1261 cagacgatgg tgcaggatat cctgctgatg aagcagaaca actttaacgc cgtgcgctgt
     1321 tcgcattatc cgaaccatcc gctgtggtac acgctgtgcg accgctacgg cctgtatgtg
     1381 gtggatgaag ccaatattga aacccacggc atggtgccaa tgaatcgtct gaccgatgat
     1441 ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat
     1501 cacccgagtg tgatcatctg gtcgctgggg aatgaatcag gccacggcgc taatcacgac
     1561 gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc
     1621 ggagccgaca ccacggccac cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac
     1681 cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct acctggagag
     1741 acgcgcccgc tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc
     1801 gctaaatact ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt cgtctgggac
     1861 tgggtggatc agtcgctgat taaatatgat gaaaacggca acccgtggtc ggcttacggc
     1921 ggtgattttg gcgatacgcc gaacgatcgc cagttctgta tgaacggtct ggtctttgcc
     1981 gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc agcagcagtt tttccagttc
     2041 cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac
     2101 gagctcctgc actggatggt ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct
     2161 ctggatgtcg ctccacaagg taaacagttg attgaactgc ctgaactacc gcagccggag
     2221 agcgccgggc aactctggct cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca
     2281 gaagccgggc acatcagcgc ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg
     2341 ctccccgccg cgtcccacgc catcccgcat ctgaccacca gcgaaatgga tttttgcatc
     2401 gagctgggta ataagcgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg
     2461 attggcgata aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg
     2521 gataacgaca ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc
     2581 tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca
     2641 cttgctgatg cggtgctgat tacgaccgct cacgcgtggc agcatcaggg gaaaacctta
     2701 tttatcagcc ggaaaaccta ccggattgat ggtagtggtc aaatggcgat taccgttgat
     2761 gttgaagtgg cgagcgatac accgcatccg gcgcggattg gcctgaactg ccagctggcg
     2821 caggtagcag agcgggtaaa ctggctcgga ttagggccgc aagaaaacta tcccgaccgc
     2881 cttactgccg cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac
     2941 gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac
     3001 cagtggcgcg gcgacttcca gttcaacatc agccgctaca gtcaacagca actgatggaa
     3061 accagccatc gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc
     3121 catatgggga ttggtggcga cgactcctgg agcccgtcag tatcggcgga attccagctg
     3181 agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataataata accgggcagg
     3241 ccatgtctgc ccgtatttcg cgtaaggaaa tccattatgt actatttaaa aaacacaaac
     3301 ttttggatgt tcggtttatt ctttttcttt tactttttta tcatgggagc ctacttcccg
     3361 tttttcccga tttggctaca tgacatcaac catatcagca aaagtgatac gggtattatt
     3421 tttgccgcta tttctctgtt ctcgctatta ttccaaccgc tgtttggtct gctttctgac
     3481 aaactcgggc tgcgcaaata cctgctgtgg attattaccg gcatgttagt gatgtttgcg
     3541 ccgttcttta tttttatctt cgggccactg ttacaataca acattttagt aggatcgatt
     3601 gttggtggta tttatctagg cttttgtttt aacgccggtg cgccagcagt agaggcattt
     3661 attgagaaag tcagccgtcg cagtaatttc gaatttggtc gcgcgcggat gtttggctgt
     3721 gttggctggg cgctgtgtgc ctcgattgtc ggcatcatgt tcaccatcaa taatcagttt
     3781 gttttctggc tgggctctgg ctgtgcactc atcctcgccg ttttactctt tttcgccaaa
     3841 acggatgcgc cctcttctgc cacggttgcc aatgcggtag gtgccaacca ttcggcattt
     3901 agccttaagc tggcactgga actgttcaga cagccaaaac tgtggttttt gtcactgtat
     3961 gttattggcg tttcctgcac ctacgatgtt tttgaccaac agtttgctaa tttctttact
     4021 tcgttctttg ctaccggtga acagggtacg cgggtatttg gctacgtaac gacaatgggc
     4081 gaattactta acgcctcgat tatgttcttt gcgccactga tcattaatcg catcggtggg
     4141 aaaaacgccc tgctgctggc tggcactatt atgtctgtac gtattattgg ctcatcgttc
     4201 gccacctcag cgctggaagt ggttattctg aaaacgctgc atatgtttga agtaccgttc
     4261 ctgctggtgg gctgctttaa atatattacc agccagtttg aagtgcgttt ttcagcgacg
     4321 atttatctgg tctgtttctg cttctttaag caactggcga tgatttttat gtctgtactg
     4381 gcgggcaata tgtatgaaag catcggtttc cagggcgctt atctggtgct gggtctggtg
     4441 gcgctgggct tcaccttaat ttccgtgttc acgcttagcg gccccggccc gctttccctg
     4501 ctgcgtcgtc aggtgaatga agtcgcttaa gcaatcaatg tcggatgcgg cgcgacgctt
     4561 atccgaccaa catatcataa cggagtgatc gcattgaaca tgccaatgac cgaaagaata
     4621 agagcaggca agctatttac cgatatgtgc gaaggcttac cggaaaaaag acttcgtggg
     4681 aaaagcatgc gatttaagca atcaatgtcg gatgcggcgc gacgcttatc cgaccaaaaa
     4741 aaccgggcaa tgcccggttt tttttaatta ttcctgaatc gccccatcat ccagccagaa
     4801 agtgagggag ccacggttga tgagagcttt gttgtaggtg gaccagttgg tgattttgaa
     4861 cttttgcttt gccacggaac ggtctgcgtt gtcgggaaga tgcgtgatct gatccttcaa
     4921 ctcagcaaaa gttcgattta ttcaacaaag ccgccgtccc gtcaagtcag cgtaatgctc
     4981 tgccagtgtt acaaccaatt aaccaattct gattagaaaa actcatcgag catcaaatga
     5041 aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt
     5101 aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct
     5161 gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
     5221 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagctta
     5281 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
     5341 gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg
     5401 ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc
     5461 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc
     5521 ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
     5581 gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca
     5641 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac
     5701 aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
     5761 aaatcagcat ccatgttgga atttaatcgc ggcctcgagc aagacgtttc ccgttgaata
     5821 tggctcataa caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat
     5881 gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacaac gtggctttcc
     5941 cccccccccc cgagctcatc gatttcgaac cccggccacg atgcgtccgg cgtagaggat
     6001 ctgaagatca gcagttcaac ctgttgatag tacgtactaa gctctcatgt ttcacgtact
     6061 aagctctcat gtttaacgta ctaagctctc atgtttaacg aactaaaccc tcatggctaa
     6121 cgtactaagc tctcatggct aacgtactaa gctctcatgt ttcacgtact aagctctcat
     6181 gtttgaacaa taaaattaat ataaatcagc aacttaaata gcctctaagg ttttaagttt
     6241 tataagaaaa aaaagaatat ataaggcttt taaa
//
