LOCUS       V008577                 6290 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008577
VERSION     V008577
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Ellermeier CD, Janakiraman A, Slauch JM.
  TITLE     Construction of targeted single copy lac fusions using lambda Red
            and FLP-mediated site-specific recombination in bacteria
  JOURNAL   Gene 290 (1-2), 153-161 (2002)
   PUBMED   12062810
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   Ellermeier CD, Janakiraman A, Slauch JM.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2001) Microbiology, University of Illinois
            Urbana-Champaign, 601 S. Goodwin Ave. B103 CLSL, Urbana, IL 61801,
            USA
REFERENCE   3  (bases 1 to 6290)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6290)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 290
            (1-2), 153-161 (2002)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-NOV-2001) Microbiology, University of Illinois Urbana-Champaign,
            601 S. Goodwin Ave. B103 CLSL, Urbana, IL 61801, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    122..155
                     /label="FRT (minimal)"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     CDS             167..3238
                     /label="lacZ"
                     /note="beta-galactosidase"
     CDS             3293..4543
                     /gene="lacY"
                     /label="Lactose permease"
                     /note="Lactose permease from Escherichia coli (strain K12).
                     Accession#: P02920"
     regulatory      4752..4781
                     /note="histidine bi-directional rho-independent terminator
                     from Salmonella typhimurium"
                     /regulatory_class="terminator"
     CDS             complement(5032..5844)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      6014..6290
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 gcttttaagg tttaacggtt gtggacaaca agccagggat gtaacgcact gagaagccct
       61 tagagcctct caaagcaatt ttgagtgaca caggaacact taacggctga catggggggg
      121 ggaagttcct attctctaga aagaatagga acttccccaa acagctatga ccatgattac
      181 ggattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
      241 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
      301 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc
      361 ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt
      421 cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtaac
      481 ctatcccatt acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc
      541 gctcacattt aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga
      601 tggcgttaac tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt acggccagga
      661 cagtcgtttg ccgtctgaat ttgacctgag cgcattttta cgcgccggag aaaaccgcct
      721 cgcggtgatg gtgctgcgtt ggagtgacgg cagttatctg gaagatcagg atatgtggcg
      781 gatgagcggc attttccgtg acgtctcgtt gctgcataaa ccgactacac aaatcagcga
      841 tttccatgtt gccactcgct ttaatgatga tttcagccgc gctgtactgg aggctgaagt
      901 tcagatgtgc ggcgagttgc gtgactacct acgggtaaca gtttctttat ggcagggtga
      961 aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg
     1021 ttatgccgat cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga
     1081 aatcccgaat ctctatcgtg cggtggttga actgcacacc gccgacggca cgctgattga
     1141 agcagaagcc tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct
     1201 gaacggcaag ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg
     1261 tcaggtcatg gatgagcaga cgatggtgca ggatatcctg ctgatgaagc agaacaactt
     1321 taacgccgtg cgctgttcgc attatccgaa ccatccgctg tggtacacgc tgtgcgaccg
     1381 ctacggcctg tatgtggtgg atgaagccaa tattgaaacc cacggcatgg tgccaatgaa
     1441 tcgtctgacc gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt
     1501 gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg ctggggaatg aatcaggcca
     1561 cggcgctaat cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt
     1621 gcagtatgaa ggcggcggag ccgacaccac ggccaccgat attatttgcc cgatgtacgc
     1681 gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa tggtccatca aaaaatggct
     1741 ttcgctacct ggagagacgc gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa
     1801 cagtcttggc ggtttcgcta aatactggca ggcgtttcgt cagtatcccc gtttacaggg
     1861 cggcttcgtc tgggactggg tggatcagtc gctgattaaa tatgatgaaa acggcaaccc
     1921 gtggtcggct tacggcggtg attttggcga tacgccgaac gatcgccagt tctgtatgaa
     1981 cggtctggtc tttgccgacc gcacgccgca tccagcgctg acggaagcaa aacaccagca
     2041 gcagtttttc cagttccgtt tatccgggca aaccatcgaa gtgaccagcg aatacctgtt
     2101 ccgtcatagc gataacgagc tcctgcactg gatggtggcg ctggatggta agccgctggc
     2161 aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg aactgcctga
     2221 actaccgcag ccggagagcg ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa
     2281 cgcgaccgca tggtcagaag ccgggcacat cagcgcctgg cagcagtggc gtctggcgga
     2341 aaacctcagt gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga
     2401 aatggatttt tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt
     2461 tctttcacag atgtggattg gcgataaaaa acaactgctg acgccgctgc gcgatcagtt
     2521 cacccgtgca ccgctggata acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa
     2581 cgcctgggtc gaacgctgga aggcggcggg ccattaccag gccgaagcag cgttgttgca
     2641 gtgcacggca gatacacttg ctgatgcggt gctgattacg accgctcacg cgtggcagca
     2701 tcaggggaaa accttattta tcagccggaa aacctaccgg attgatggta gtggtcaaat
     2761 ggcgattacc gttgatgttg aagtggcgag cgatacaccg catccggcgc ggattggcct
     2821 gaactgccag ctggcgcagg tagcagagcg ggtaaactgg ctcggattag ggccgcaaga
     2881 aaactatccc gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga
     2941 catgtatacc ccgtacgtct tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt
     3001 gaattatggc ccacaccagt ggcgcggcga cttccagttc aacatcagcc gctacagtca
     3061 acagcaactg atggaaacca gccatcgcca tctgctgcac gcggaagaag gcacatggct
     3121 gaatatcgac ggtttccata tggggattgg tggcgacgac tcctggagcc cgtcagtatc
     3181 ggcggaattc cagctgagcg ccggtcgcta ccattaccag ttggtctggt gtcaaaaata
     3241 ataataaccg ggcaggccat gtctgcccgt atttcgcgta aggaaatcca ttatgtacta
     3301 tttaaaaaac acaaactttt ggatgttcgg tttattcttt ttcttttact tttttatcat
     3361 gggagcctac ttcccgtttt tcccgatttg gctacatgac atcaaccata tcagcaaaag
     3421 tgatacgggt attatttttg ccgctatttc tctgttctcg ctattattcc aaccgctgtt
     3481 tggtctgctt tctgacaaac tcgggctgcg caaatacctg ctgtggatta ttaccggcat
     3541 gttagtgatg tttgcgccgt tctttatttt tatcttcggg ccactgttac aatacaacat
     3601 tttagtagga tcgattgttg gtggtattta tctaggcttt tgttttaacg ccggtgcgcc
     3661 agcagtagag gcatttattg agaaagtcag ccgtcgcagt aatttcgaat ttggtcgcgc
     3721 gcggatgttt ggctgtgttg gctgggcgct gtgtgcctcg attgtcggca tcatgttcac
     3781 catcaataat cagtttgttt tctggctggg ctctggctgt gcactcatcc tcgccgtttt
     3841 actctttttc gccaaaacgg atgcgccctc ttctgccacg gttgccaatg cggtaggtgc
     3901 caaccattcg gcatttagcc ttaagctggc actggaactg ttcagacagc caaaactgtg
     3961 gtttttgtca ctgtatgtta ttggcgtttc ctgcacctac gatgtttttg accaacagtt
     4021 tgctaatttc tttacttcgt tctttgctac cggtgaacag ggtacgcggg tatttggcta
     4081 cgtaacgaca atgggcgaat tacttaacgc ctcgattatg ttctttgcgc cactgatcat
     4141 taatcgcatc ggtgggaaaa acgccctgct gctggctggc actattatgt ctgtacgtat
     4201 tattggctca tcgttcgcca cctcagcgct ggaagtggtt attctgaaaa cgctgcatat
     4261 gtttgaagta ccgttcctgc tggtgggctg ctttaaatat attaccagcc agtttgaagt
     4321 gcgtttttca gcgacgattt atctggtctg tttctgcttc tttaagcaac tggcgatgat
     4381 ttttatgtct gtactggcgg gcaatatgta tgaaagcatc ggtttccagg gcgcttatct
     4441 ggtgctgggt ctggtggcgc tgggcttcac cttaatttcc gtgttcacgc ttagcggccc
     4501 cggcccgctt tccctgctgc gtcgtcaggt gaatgaagtc gcttaagcaa tcaatgtcgg
     4561 atgcggcgcg acgcttatcc gaccaacata tcataacgga gtgatcgcat tgaacatgcc
     4621 aatgaccgaa agaataagag caggcaagct atttaccgat atgtgcgaag gcttaccgga
     4681 aaaaagactt cgtgggaaaa gcatgcgatt taagcaatca atgtcggatg cggcgcgacg
     4741 cttatccgac caaaaaaacc gggcaatgcc cggttttttt taattattcc tgaatcgccc
     4801 catcatccag ccagaaagtg agggagccac ggttgatgag agctttgttg taggtggacc
     4861 agttggtgat tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg
     4921 tgatctgatc cttcaactca gcaaaagttc gatttattca acaaagccgc cgtcccgtca
     4981 agtcagcgta atgctctgcc agtgttacaa ccaattaacc aattctgatt agaaaaactc
     5041 atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg
     5101 aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag
     5161 atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc
     5221 ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga
     5281 gaatggcaaa agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc
     5341 gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag
     5401 acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg
     5461 caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac
     5521 ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg
     5581 gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat
     5641 ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc
     5701 atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc
     5761 ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc tcgagcaaga
     5821 cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag
     5881 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
     5941 cacaacgtgg ctttcccccc cccccccgag ctcatcgatt tcgaaccccg gccacgatgc
     6001 gtccggcgta gaggatctga agatcagcag ttcaacctgt tgatagtacg tactaagctc
     6061 tcatgtttca cgtactaagc tctcatgttt aacgtactaa gctctcatgt ttaacgaact
     6121 aaaccctcat ggctaacgta ctaagctctc atggctaacg tactaagctc tcatgtttca
     6181 cgtactaagc tctcatgttt gaacaataaa attaatataa atcagcaact taaatagcct
     6241 ctaaggtttt aagttttata agaaaaaaaa gaatatataa ggcttttaaa
//
